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Protein

Vascular endothelial growth factor A-A

Gene

vegfaa

Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis, and induces permeabilization of blood vessels. Acts both upstream of kdr and tie1 to stimulate endothelial cell differentiation, and upstream of gata1 to stimulate hematopoietic cell differentiation.By similarity2 Publications

GO - Molecular functioni

  • growth factor activity Source: UniProtKB
  • heparin binding Source: InterPro
  • vascular endothelial growth factor receptor binding Source: UniProtKB

GO - Biological processi

  • activation of phospholipase C activity Source: ZFIN
  • angioblast cell migration from lateral mesoderm to midline Source: ZFIN
  • angiogenesis Source: UniProtKB
  • artery morphogenesis Source: ZFIN
  • blood vessel development Source: ZFIN
  • blood vessel endothelial cell proliferation involved in sprouting angiogenesis Source: ZFIN
  • endocardial progenitor cell migration to the midline involved in heart field formation Source: ZFIN
  • hemopoiesis Source: UniProtKB
  • motor neuron axon guidance Source: ZFIN
  • patterning of blood vessels Source: ZFIN
  • phosphorylation Source: UniProtKB
  • positive regulation of angiogenesis Source: ZFIN
  • positive regulation of cell division Source: UniProtKB-KW
  • positive regulation of endothelial cell proliferation Source: UniProtKB
  • positive regulation of focal adhesion assembly Source: UniProtKB
  • positive regulation of peptidyl-tyrosine phosphorylation Source: UniProtKB
  • positive regulation of protein complex assembly Source: UniProtKB
  • pronephros development Source: ZFIN
  • protein kinase B signaling Source: ZFIN
  • regulation of angioblast cell migration involved in selective angioblast sprouting Source: ZFIN
  • regulation of heart contraction Source: ZFIN
  • regulation of vasculature development Source: ZFIN
  • response to glucose Source: ZFIN
  • sprouting angiogenesis Source: ZFIN
  • thyroid gland development Source: ZFIN
  • vascular endothelial growth factor receptor signaling pathway Source: ZFIN
  • vasculature development Source: ZFIN
  • vasculogenesis Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor, Mitogen

Keywords - Biological processi

Angiogenesis, Differentiation

Enzyme and pathway databases

ReactomeiR-DRE-114608. Platelet degranulation.
R-DRE-194313. VEGF ligand-receptor interactions.
R-DRE-195399. VEGF binds to VEGFR leading to receptor dimerization.
R-DRE-3928663. EPHA-mediated growth cone collapse.
R-DRE-4420097. VEGFA-VEGFR2 Pathway.
R-DRE-5218921. VEGFR2 mediated cell proliferation.

Names & Taxonomyi

Protein namesi
Recommended name:
Vascular endothelial growth factor A-A
Short name:
VEGF-A-A
Gene namesi
Name:vegfaa
Synonyms:vegf, vegfa
OrganismiDanio rerio (Zebrafish) (Brachydanio rerio)Imported
Taxonomic identifieri7955 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiActinopterygiiNeopterygiiTeleosteiOstariophysiCypriniformesCyprinidaeDanio
Proteomesi
  • UP000000437 Componenti: Chromosome 16

Organism-specific databases

ZFINiZDB-GENE-990415-273. vegfaa.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: UniProtKB
  • intracellular Source: GOC
  • membrane Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Sequence analysisAdd
BLAST
Chaini24 – 188165Vascular endothelial growth factor A-APRO_0000023394Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi49 ↔ 91By similarity
Disulfide bondi74 – 74InterchainBy similarity
Disulfide bondi80 ↔ 125By similarity
Disulfide bondi83 – 83InterchainBy similarity
Disulfide bondi84 ↔ 127By similarity
Glycosylationi98 – 981N-linked (GlcNAc...)Curated

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiO73682.

Expressioni

Tissue specificityi

Predominantly expressed in regions associated with active vascularization. From 15-16 hours post-fertilization (hpf), expressed in the anterior forebrain, the mesoderm underlying and lateral to the anterior hindbrain, the mesoderm underlying and lateral to the posterior hindbrain, and in the ventral medial portions of the somites. By 30-36 hpf, expression in the somites is decreased, while strong expression is observed in the region of the developing glomeruli and in the anterior portion of the pronephric ducts, the pharyngeal arches, and the brain. By 72 hpf, expression remains only in the pronephros region.

Developmental stagei

Expressed both maternally and zygotically. Present throughout embryonic development, and in adults.2 Publications

Gene expression databases

BgeeiENSDARG00000045971.

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Isoform VEGF165 binds kdr and kdrl.By similarity

GO - Molecular functioni

  • growth factor activity Source: UniProtKB
  • vascular endothelial growth factor receptor binding Source: UniProtKB

Protein-protein interaction databases

STRINGi7955.ENSDARP00000110575.

Structurei

3D structure databases

ProteinModelPortaliO73682.
SMRiO73682. Positions 35-132, 135-188.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PDGF/VEGF growth factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IGCM. Eukaryota.
ENOG410ZWYU. LUCA.
GeneTreeiENSGT00730000110791.
HOGENOMiHOG000230896.
HOVERGENiHBG000105.
InParanoidiO73682.
KOiK05448.
OMAiERSFCRT.
OrthoDBiEOG091G0G6M.
PhylomeDBiO73682.
TreeFamiTF319554.

Family and domain databases

Gene3Di2.10.160.10. 1 hit.
2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR027928. VEGF_C.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF14554. VEGF_C. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
SSF57593. SSF57593. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform VEGF1651 Publication (identifier: O73682-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNLVVYLIQL FLAALLHLSA VKAAHIPKEG GKSKNDVIPF MDVYKKSACK
60 70 80 90 100
TRELLVDIIQ EYPDEIEHTY IPSCVVLMRC AGCCNDEALE CVPTETRNVT
110 120 130 140 150
MEVLRVKQRV SQHNFQLSFT EHTKCECRPK AEVKAKKENH CEPCSERRKR
160 170 180
LYVQDPLTCK CSCKFTQMQC KSRQLELNER TCRCEKPR
Length:188
Mass (Da):21,756
Last modified:August 1, 1998 - v1
Checksum:iFD203334EF365D1E
GO
Isoform VEGF1211 Publication (identifier: O73682-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     139-182: Missing.

Show »
Length:144
Mass (Da):16,479
Checksum:i303E6A7407AA0832
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei139 – 18244Missing in isoform VEGF121. 1 PublicationVSP_050737Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016244 mRNA. Translation: AAC41274.1.
AF059661 mRNA. Translation: AAC14713.1.
BC162258 mRNA. Translation: AAI62258.1.
BC162588 mRNA. Translation: AAI62588.1.
RefSeqiNP_001103819.2. NM_001110349.2. [O73682-2]
NP_571483.1. NM_131408.3. [O73682-1]
UniGeneiDr.150231.
Dr.597.

Genome annotation databases

EnsembliENSDART00000167719; ENSDARP00000133415; ENSDARG00000103542. [O73682-1]
GeneIDi30682.
KEGGidre:30682.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF016244 mRNA. Translation: AAC41274.1.
AF059661 mRNA. Translation: AAC14713.1.
BC162258 mRNA. Translation: AAI62258.1.
BC162588 mRNA. Translation: AAI62588.1.
RefSeqiNP_001103819.2. NM_001110349.2. [O73682-2]
NP_571483.1. NM_131408.3. [O73682-1]
UniGeneiDr.150231.
Dr.597.

3D structure databases

ProteinModelPortaliO73682.
SMRiO73682. Positions 35-132, 135-188.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi7955.ENSDARP00000110575.

Proteomic databases

PaxDbiO73682.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSDART00000167719; ENSDARP00000133415; ENSDARG00000103542. [O73682-1]
GeneIDi30682.
KEGGidre:30682.

Organism-specific databases

CTDi30682.
ZFINiZDB-GENE-990415-273. vegfaa.

Phylogenomic databases

eggNOGiENOG410IGCM. Eukaryota.
ENOG410ZWYU. LUCA.
GeneTreeiENSGT00730000110791.
HOGENOMiHOG000230896.
HOVERGENiHBG000105.
InParanoidiO73682.
KOiK05448.
OMAiERSFCRT.
OrthoDBiEOG091G0G6M.
PhylomeDBiO73682.
TreeFamiTF319554.

Enzyme and pathway databases

ReactomeiR-DRE-114608. Platelet degranulation.
R-DRE-194313. VEGF ligand-receptor interactions.
R-DRE-195399. VEGF binds to VEGFR leading to receptor dimerization.
R-DRE-3928663. EPHA-mediated growth cone collapse.
R-DRE-4420097. VEGFA-VEGFR2 Pathway.
R-DRE-5218921. VEGFR2 mediated cell proliferation.

Miscellaneous databases

PROiO73682.

Gene expression databases

BgeeiENSDARG00000045971.

Family and domain databases

Gene3Di2.10.160.10. 1 hit.
2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR027928. VEGF_C.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF14554. VEGF_C. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
SSF57593. SSF57593. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVGFAA_DANRE
AccessioniPrimary (citable) accession number: O73682
Secondary accession number(s): B3DG34, O73822
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 7, 2004
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.