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O73556

- POLG_HPE2W

UniProt

O73556 - POLG_HPE2W

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Protein

Genome polyprotein

Gene
N/A
Organism
Human parechovirus 2 (strain Williamson) (HPeV-2) (Echovirus 23)
Status
Reviewed - Annotation score: 5 out of 5- Protein inferred from homologyi

Functioni

Protein 2A: Is not a protease.By similarity
Protein 2B: Affects membrane integrity and cause an increase in membrane permeability.By similarity
Protein 2C: Associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities (By similarity).By similarity
Protein 3A, via its hydrophobic domain, serves as membrane anchor.By similarity
Protease 3C: cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind cooperatively to the protease (By similarity).By similarity
RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals.PROSITE-ProRule annotation

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).PROSITE-ProRule annotation
Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein.
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
NTP + H2O = NDP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei775 – 7762Cleavage; by protease 2ASequence Analysis
Active sitei1549 – 15491For protease 3C activitySequence Analysis
Active sitei1580 – 15801For protease 3C activitySequence Analysis
Active sitei1669 – 16691For protease 3C activitySequence Analysis

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1184 – 11918ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. cysteine-type endopeptidase activity Source: InterPro
  3. ion channel activity Source: UniProtKB-KW
  4. RNA binding Source: UniProtKB-KW
  5. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  6. RNA helicase activity Source: InterPro
  7. structural molecule activity Source: InterPro

GO - Biological processi

  1. induction by virus of host autophagy Source: UniProtKB
  2. pore formation by virus in membrane of host cell Source: UniProtKB-KW
  3. protein oligomerization Source: UniProtKB-KW
  4. RNA-protein covalent cross-linking Source: UniProtKB-KW
  5. suppression by virus of host RIG-I activity by RIG-I proteolysis Source: UniProtKB
  6. suppression by virus of host translation initiation factor activity Source: UniProtKB
  7. transcription, DNA-templated Source: InterPro
  8. viral entry into host cell Source: UniProtKB-KW
  9. viral RNA genome replication Source: InterPro
  10. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase, Viral ion channel

Keywords - Biological processi

Host-virus interaction, Ion transport, Transport, Viral attachment to host cell, Viral RNA replication, Virus entry into host cell

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Protein family/group databases

MEROPSiC03.023.

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 10 chains:
Alternative name(s):
P1AB
Virion protein 0
Alternative name(s):
P1C
Virion protein 3
Alternative name(s):
P1D
Virion protein 1
Protein 2A
Short name:
P2A
Protein 2B
Short name:
P2B
Protein 2C (EC:3.6.1.15)
Short name:
P2C
Protein 3A
Short name:
P3A
Protein 3B
Short name:
P3B
Alternative name(s):
VPg
Protease 3C (EC:3.4.22.28)
Short name:
P3C
Alternative name(s):
Picornain 3C
OrganismiHuman parechovirus 2 (strain Williamson) (HPeV-2) (Echovirus 23)
Taxonomic identifieri122962 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeParechovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000000670: Genome

Subcellular locationi

Chain Protein VP3 : Virion By similarity. Host cytoplasm Curated
Chain Protein VP1 : Virion By similarity. Host cytoplasm Curated
Chain Protein 2B : Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity).By similarity
Chain Protein 2C : Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity).By similarity
Chain Protein 3A : Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity).By similarity
Chain RNA-directed RNA polymerase 3D-POL : Host cytoplasmic vesicle membrane Curated; Peripheral membrane protein Curated; Cytoplasmic side Curated
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum (By similarity).By similarity

GO - Cellular componenti

  1. host cell cytoplasmic vesicle Source: UniProtKB-KW
  2. integral to membrane of host cell Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
  4. viral capsid Source: UniProtKB-KW
  5. viral genome Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Host cytoplasmic vesicle, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Protein VP0Sequence AnalysisPRO_0000039750Add
BLAST
Chaini290 – 542253Protein VP3Sequence AnalysisPRO_0000039751Add
BLAST
Chaini543 – 775233Protein VP1Sequence AnalysisPRO_0000039752Add
BLAST
Chaini776 – 922147Protein 2ASequence AnalysisPRO_0000039753Add
BLAST
Chaini923 – 1044122Protein 2BSequence AnalysisPRO_0000039754Add
BLAST
Chaini1045 – 1373329Protein 2CSequence AnalysisPRO_0000039755Add
BLAST
Chaini1374 – 1490117Protein 3ASequence AnalysisPRO_0000039756Add
BLAST
Chaini1491 – 151020Protein 3BSequence AnalysisPRO_0000039757Add
BLAST
Chaini1511 – 1710200Protease 3CSequence AnalysisPRO_0000039758Add
BLAST
Chaini1711 – 2179469RNA-directed RNA polymerase 3D-POLSequence AnalysisPRO_0000039759Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1493 – 14931O-(5'-phospho-RNA)-tyrosineBy similarity

Post-translational modificationi

VPg is uridylylated by the polymerase and is covalently linked to the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase (By similarity).By similarity
Specific enzymatic cleavages yield mature proteins. All cleavages are catalyzed by P3C.

Keywords - PTMi

Covalent protein-RNA linkage, Lipoprotein, Myristate, Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliO73556.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1156 – 1317162SF3 helicasePROSITE-ProRule annotationAdd
BLAST
Domaini1944 – 2058115RdRp catalyticPROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi763 – 7653Cell attachment siteSequence Analysis

Sequence similaritiesi

Belongs to the picornaviruses polyprotein family.Curated
Contains 2 peptidase C3 domains.Curated
Contains 1 RdRp catalytic domain.PROSITE-ProRule annotation
Contains 1 SF3 helicase domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di2.60.120.20. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
IPR009419. VPP_parechovir_P3A.
IPR009407. VPP_parechovir_P3B.
[Graphical view]
PfamiPF06344. Parecho_VpG. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF06363. Picorna_P3A. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00918. CALICVIRUSNS.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O73556-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
METIKSIADM ATGVTKTIDA TINSVNEIIT NTDNASGGDI LTKVADDASN
60 70 80 90 100
ILGPNCYATT SEPENKDVVQ ATTTVNTTNL TQHPSAPTLP FTPDFSNVDT
110 120 130 140 150
FHSMAYDTTT GSKNPNKLVR LTTHAWASTL QRGHQIDHVN LPVDFWDEQR
160 170 180 190 200
KPAYGHAKYF AAVRCGFHFQ VQVNVNQGTA GSALVVYEPK PVVDYDKDLE
210 220 230 240 250
FGAFTNLPHV LMNLAETTQA DLCIPYVADT NYVKTDSSDL GQLKVYVWTP
260 270 280 290 300
LSIPSGSSNQ VDVTILGSLL QLDFQNPRVY GQNVDIYDTA PSKPIPLRKT
310 320 330 340 350
KYLTMSTKYK WTRNKVDIAE GPGSMNMANV LSTTAAQSVA LVGERAFYDP
360 370 380 390 400
RTAGSKSRFD DLVKISQLFS VMADSTTPSA NHGIDQKGYF KWSANSDPQA
410 420 430 440 450
IVHRNLVHLN LFPNLKVFEN SYSYFRGSLI IRLSVYASTF NRGRLNGFFP
460 470 480 490 500
NSSTDETSEI DNAIYTICDI GSDNSFEITI PYSFSTWMRK THGKPIGLFQ
510 520 530 540 550
IEVLNRLTYN YSSPNEVYCI VQGKMGQDAK FFCPTGSLVT FQNSWGSQMD
560 570 580 590 600
LTDPLCIEDS VEDCKQTITP TELGLTSAQD DGPLGNDKPN YFLNFKSMNV
610 620 630 640 650
DIFTVSHTKV DNIFGRAWFA HVHDFTNDGL WRQGLEFPKE GHGALSLLFA
660 670 680 690 700
YFTGELNIHV LFLSDRGFLR VGHTYDTETN RTNFLSSSGI ITVPAGEQMT
710 720 730 740 750
LSVPSYSNKP LRTVRSSNAL GYLLCKPLLT GTSSGRIEIF LSLRCPNFFF
760 770 780 790 800
PLPAPKPATR KYRGDLATWS DQSPYGRQGK KQLMKLAYLD RGFYKHYGIV
810 820 830 840 850
VGDDVYQLDS DDIFKTALTG KAKFTKTRLT PDWVVEEECE LDYFRIKYLE
860 870 880 890 900
SSVNSEHIFS VDNNCETIAK DIFGSHSLSQ HQQIGLIGTI LLTAGLMSTI
910 920 930 940 950
KTPVNPTTIK EFFNHAIEGD EQGLSLLVQK CTTFFSSAAT ELLDNDLVKF
960 970 980 990 1000
IIKILVRILC YMVLYCHKPN ILTTACLSTL LVMDVTSSSV LSPSCKALMQ
1010 1020 1030 1040 1050
CLMDGDVKKL AEVVAESMSN TDDDEIKEQI CDTVKYTKQI LSNQGPFKGF
1060 1070 1080 1090 1100
NEISTAFRHI DWWIQTLLKI KDMVLSVFKP SVEKRAVEWL ERNKEHVCSI
1110 1120 1130 1140 1150
LDYASDIIVK SKDQTKMKTQ EFYQRYNDCL SKFKPIMAMC FRSCHNSISN
1160 1170 1180 1190 1200
TVYRLFQELA RIPNRMATQN DLIRVEPIGI WIQGEPGQGK SFLTHTLSKQ
1210 1220 1230 1240 1250
LQKTCGLQGI YTNPTASEFM DGYDNQDIHL IDDLGQTRKE RDIEMLCNCI
1260 1270 1280 1290 1300
SSDPDIVPMA HLEEKGKFYT SKLVIATTNK PDFSSTVLLD SGALRRRFPY
1310 1320 1330 1340 1350
IMHIRAAKHY SKSGKLNVSQ AMPHMSTGEC WEVSKNGRDW ETLKLKELID
1360 1370 1380 1390 1400
KITVDYKERI ANYNTWKKQL EDQTLDDLDD AVSYIKHNYP DAIPYIDEYL
1410 1420 1430 1440 1450
NIEMSTLIEQ MEAFIEPKPS VFKCFASRVG DKIKEASREV VKWFSDKLKS
1460 1470 1480 1490 1500
MLNFVERNKA WLTVVSAVTS AIGILLLVTK IFKKEESKDE RAYNPTLPVA
1510 1520 1530 1540 1550
KPKGTFPVSQ REFKNEAPYD GQLEHIISQM AYITGSTTGH ITHCAGYQHD
1560 1570 1580 1590 1600
EIILHGHSIK YLEQEEELTL HYKNKVFPIE QPSVTQVTLG GKPMDLAIVK
1610 1620 1630 1640 1650
CKLPFRFKKN SKYYTNKIGT ESMLIWMTEQ GIITKEVQRV HHSGGIKTRE
1660 1670 1680 1690 1700
GTESTKTISY TVKSCKGMCG GLLISKVEGN FKILGMHIAG NGEMGVAIPF
1710 1720 1730 1740 1750
NFLKNDMSDQ GIVTEVTPIQ PMYINTKSQI HKSPVYGAVE VKMGPAVLSK
1760 1770 1780 1790 1800
SDTRLEEPVD CLVKKSASKY RVNKFQVNNE LWQGVKACVK SKFREIFGVN
1810 1820 1830 1840 1850
GIVDMKTAIL GTSHVNSMDL STSAGYSFVK SGYKKKDLIC LEPFSVSPML
1860 1870 1880 1890 1900
EKLVQEKFHN LLKGNQITTI FNTCLKDELR KLDKIATGKT RCIEACEIDY
1910 1920 1930 1940 1950
CIVYRMIMME IYDKIYQTPC YYSGLAVGIN PYRDWHFMIN ALNDYNYEMD
1960 1970 1980 1990 2000
YSQYDGSLSS MLLWEAVQVL AYCHDSPDLV MQLHKPVIDS DHVVFNERWL
2010 2020 2030 2040 2050
IHGGMPSGSP CTTVLNSLCN LMMCIYTTNL ISPGIDCLPI VYGDDVILSL
2060 2070 2080 2090 2100
DKEIEPERLQ SIMAESFGAE VTGSRKDEPP SLKPRMEVEF LKRKPGYFPE
2110 2120 2130 2140 2150
STFIVGKLDT ENMIQHLMWM KNFSTFKQQL QSYLMELCLH GKDTYQHYVK
2160 2170
ILNPYLKEWN IPVDDYEVVI GKLVPMVFD
Length:2,179
Mass (Da):245,874
Last modified:August 1, 1998 - v1
Checksum:i96803C0BB8856664
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005695 Genomic RNA. Translation: CAA06679.1.
RefSeqiNP_046804.1. NC_001897.1.

Genome annotation databases

GeneIDi1403455.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005695 Genomic RNA. Translation: CAA06679.1 .
RefSeqi NP_046804.1. NC_001897.1.

3D structure databases

ProteinModelPortali O73556.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C03.023.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1403455.

Family and domain databases

Gene3Di 2.60.120.20. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
IPR009419. VPP_parechovir_P3A.
IPR009407. VPP_parechovir_P3B.
[Graphical view ]
Pfami PF06344. Parecho_VpG. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF06363. Picorna_P3A. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00918. CALICVIRUSNS.
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular analysis of human parechovirus type 2 (formerly echovirus 23)."
    Ghazi F., Hughes P.J., Hyypiae T., Stanway G.
    J. Gen. Virol. 79:2641-2650(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOLG_HPE2W
AccessioniPrimary (citable) accession number: O73556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3