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O73556

- POLG_HPE2W

UniProt

O73556 - POLG_HPE2W

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Protein
Genome polyprotein
Gene
N/A
Organism
Human parechovirus 2 (strain Williamson) (HPeV-2) (Echovirus 23)
Status
Reviewed - Annotation score: 5 out of 5 - Protein inferred from homologyi

Functioni

Protein 2A: Is not a protease By similarity.
Protein 2B: Affects membrane integrity and cause an increase in membrane permeability By similarity.
Protein 2C: Associates with and induces structural rearrangements of intracellular membranes. It displays RNA-binding, nucleotide binding and NTPase activities By similarity.
Protein 3A, via its hydrophobic domain, serves as membrane anchor By similarity.
Protease 3C: cysteine protease that generates mature viral proteins from the precursor polyprotein. In addition to its proteolytic activity, it binds to viral RNA, and thus influences viral genome replication. RNA and substrate bind cooperatively to the protease By similarity.
RNA-directed RNA polymerase 3D-POL replicates genomic and antigenomic RNA by recognizing replications specific signals By similarity.

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Selective cleavage of Tyr-|-Gly bond in the picornavirus polyprotein.
Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.
NTP + H2O = NDP + phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei775 – 7762Cleavage; by protease 2A Reviewed prediction
Active sitei1549 – 15491For protease 3C activity Reviewed prediction
Active sitei1580 – 15801For protease 3C activity Reviewed prediction
Active sitei1669 – 16691For protease 3C activity Reviewed prediction

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1184 – 11918ATP Reviewed prediction

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. RNA binding Source: UniProtKB-KW
  3. RNA helicase activity Source: InterPro
  4. RNA-directed RNA polymerase activity Source: UniProtKB-KW
  5. cysteine-type endopeptidase activity Source: InterPro
  6. ion channel activity Source: UniProtKB-KW
  7. structural molecule activity Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. RNA-protein covalent cross-linking Source: UniProtKB-KW
  2. induction by virus of host autophagy Source: UniProtKB
  3. pore formation by virus in membrane of host cell Source: UniProtKB-KW
  4. protein oligomerization Source: UniProtKB-KW
  5. suppression by virus of host RIG-I activity by RIG-I proteolysis Source: UniProtKB
  6. suppression by virus of host translation initiation factor activity Source: UniProtKB
  7. transcription, DNA-templated Source: InterPro
  8. viral RNA genome replication Source: InterPro
  9. viral entry into host cell Source: UniProtKB-KW
  10. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Helicase, Hydrolase, Ion channel, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Thiol protease, Transferase, Viral ion channel

Keywords - Biological processi

Host-virus interaction, Ion transport, Transport, Viral attachment to host cell, Viral RNA replication, Virus entry into host cell

Keywords - Ligandi

ATP-binding, Nucleotide-binding, RNA-binding

Protein family/group databases

MEROPSiC03.023.

Names & Taxonomyi

Protein namesi
Recommended name:
Genome polyprotein
Cleaved into the following 10 chains:
Alternative name(s):
P1AB
Virion protein 0
Alternative name(s):
P1C
Virion protein 3
Alternative name(s):
P1D
Virion protein 1
Protein 2A
Short name:
P2A
Protein 2B
Short name:
P2B
Protein 2C (EC:3.6.1.15)
Short name:
P2C
Protein 3A
Short name:
P3A
Protein 3B
Short name:
P3B
Alternative name(s):
VPg
Protease 3C (EC:3.4.22.28)
Short name:
P3C
Alternative name(s):
Picornain 3C
OrganismiHuman parechovirus 2 (strain Williamson) (HPeV-2) (Echovirus 23)
Taxonomic identifieri122962 [NCBI]
Taxonomic lineageiVirusesssRNA positive-strand viruses, no DNA stagePicornaviralesPicornaviridaeParechovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000000670: Genome

Subcellular locationi

Chain Protein VP3 : Virion By similarity. Host cytoplasm Reviewed prediction
Chain Protein VP1 : Virion By similarity. Host cytoplasm Reviewed prediction
Chain Protein 2B : Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Reviewed prediction
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum By similarity.
Chain Protein 2C : Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Reviewed prediction
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum By similarity.
Chain Protein 3A : Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Reviewed prediction
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum By similarity.
Chain Protein 3B : Virion Reviewed prediction
Chain Protease 3C : Host cytoplasm Reviewed prediction
Chain RNA-directed RNA polymerase 3D-POL : Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side Reviewed prediction
Note: Probably localizes to the surface of intracellular membrane vesicles that are induced after virus infection as the site for viral RNA replication. These vesicles are derived from the endoplasmic reticulum By similarity.

GO - Cellular componenti

  1. host cell cytoplasmic vesicle membrane Source: UniProtKB-SubCell
  2. integral to membrane of host cell Source: UniProtKB-KW
  3. membrane Source: UniProtKB-KW
  4. viral capsid Source: UniProtKB-KW
  5. viral genome Source: InterPro
Complete GO annotation...

Keywords - Cellular componenti

Capsid protein, Host cytoplasm, Host cytoplasmic vesicle, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 289289Protein VP0 Reviewed prediction
PRO_0000039750Add
BLAST
Chaini290 – 542253Protein VP3 Reviewed prediction
PRO_0000039751Add
BLAST
Chaini543 – 775233Protein VP1 Reviewed prediction
PRO_0000039752Add
BLAST
Chaini776 – 922147Protein 2A Reviewed prediction
PRO_0000039753Add
BLAST
Chaini923 – 1044122Protein 2B Reviewed prediction
PRO_0000039754Add
BLAST
Chaini1045 – 1373329Protein 2C Reviewed prediction
PRO_0000039755Add
BLAST
Chaini1374 – 1490117Protein 3A Reviewed prediction
PRO_0000039756Add
BLAST
Chaini1491 – 151020Protein 3B Reviewed prediction
PRO_0000039757Add
BLAST
Chaini1511 – 1710200Protease 3C Reviewed prediction
PRO_0000039758Add
BLAST
Chaini1711 – 2179469RNA-directed RNA polymerase 3D-POL Reviewed prediction
PRO_0000039759Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1493 – 14931O-(5'-phospho-RNA)-tyrosine By similarity

Post-translational modificationi

VPg is uridylylated by the polymerase and is covalently linked to the 5'-end of genomic RNA. This uridylylated form acts as a nucleotide-peptide primer for the polymerase By similarity.
Specific enzymatic cleavages yield mature proteins. All cleavages are catalyzed by P3C.

Keywords - PTMi

Covalent protein-RNA linkage, Lipoprotein, Myristate, Phosphoprotein

Structurei

3D structure databases

ProteinModelPortaliO73556.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1156 – 1317162SF3 helicase
Add
BLAST
Domaini1944 – 2058115RdRp catalytic
Add
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi763 – 7653Cell attachment site Reviewed prediction

Sequence similaritiesi

Contains 2 peptidase C3 domains.

Family and domain databases

Gene3Di2.60.120.20. 2 hits.
3.40.50.300. 1 hit.
InterProiIPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
IPR009419. VPP_parechovir_P3A.
IPR009407. VPP_parechovir_P3B.
[Graphical view]
PfamiPF06344. Parecho_VpG. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF06363. Picorna_P3A. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view]
PRINTSiPR00918. CALICVIRUSNS.
SUPFAMiSSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEiPS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O73556-1 [UniParc]FASTAAdd to Basket

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METIKSIADM ATGVTKTIDA TINSVNEIIT NTDNASGGDI LTKVADDASN     50
ILGPNCYATT SEPENKDVVQ ATTTVNTTNL TQHPSAPTLP FTPDFSNVDT 100
FHSMAYDTTT GSKNPNKLVR LTTHAWASTL QRGHQIDHVN LPVDFWDEQR 150
KPAYGHAKYF AAVRCGFHFQ VQVNVNQGTA GSALVVYEPK PVVDYDKDLE 200
FGAFTNLPHV LMNLAETTQA DLCIPYVADT NYVKTDSSDL GQLKVYVWTP 250
LSIPSGSSNQ VDVTILGSLL QLDFQNPRVY GQNVDIYDTA PSKPIPLRKT 300
KYLTMSTKYK WTRNKVDIAE GPGSMNMANV LSTTAAQSVA LVGERAFYDP 350
RTAGSKSRFD DLVKISQLFS VMADSTTPSA NHGIDQKGYF KWSANSDPQA 400
IVHRNLVHLN LFPNLKVFEN SYSYFRGSLI IRLSVYASTF NRGRLNGFFP 450
NSSTDETSEI DNAIYTICDI GSDNSFEITI PYSFSTWMRK THGKPIGLFQ 500
IEVLNRLTYN YSSPNEVYCI VQGKMGQDAK FFCPTGSLVT FQNSWGSQMD 550
LTDPLCIEDS VEDCKQTITP TELGLTSAQD DGPLGNDKPN YFLNFKSMNV 600
DIFTVSHTKV DNIFGRAWFA HVHDFTNDGL WRQGLEFPKE GHGALSLLFA 650
YFTGELNIHV LFLSDRGFLR VGHTYDTETN RTNFLSSSGI ITVPAGEQMT 700
LSVPSYSNKP LRTVRSSNAL GYLLCKPLLT GTSSGRIEIF LSLRCPNFFF 750
PLPAPKPATR KYRGDLATWS DQSPYGRQGK KQLMKLAYLD RGFYKHYGIV 800
VGDDVYQLDS DDIFKTALTG KAKFTKTRLT PDWVVEEECE LDYFRIKYLE 850
SSVNSEHIFS VDNNCETIAK DIFGSHSLSQ HQQIGLIGTI LLTAGLMSTI 900
KTPVNPTTIK EFFNHAIEGD EQGLSLLVQK CTTFFSSAAT ELLDNDLVKF 950
IIKILVRILC YMVLYCHKPN ILTTACLSTL LVMDVTSSSV LSPSCKALMQ 1000
CLMDGDVKKL AEVVAESMSN TDDDEIKEQI CDTVKYTKQI LSNQGPFKGF 1050
NEISTAFRHI DWWIQTLLKI KDMVLSVFKP SVEKRAVEWL ERNKEHVCSI 1100
LDYASDIIVK SKDQTKMKTQ EFYQRYNDCL SKFKPIMAMC FRSCHNSISN 1150
TVYRLFQELA RIPNRMATQN DLIRVEPIGI WIQGEPGQGK SFLTHTLSKQ 1200
LQKTCGLQGI YTNPTASEFM DGYDNQDIHL IDDLGQTRKE RDIEMLCNCI 1250
SSDPDIVPMA HLEEKGKFYT SKLVIATTNK PDFSSTVLLD SGALRRRFPY 1300
IMHIRAAKHY SKSGKLNVSQ AMPHMSTGEC WEVSKNGRDW ETLKLKELID 1350
KITVDYKERI ANYNTWKKQL EDQTLDDLDD AVSYIKHNYP DAIPYIDEYL 1400
NIEMSTLIEQ MEAFIEPKPS VFKCFASRVG DKIKEASREV VKWFSDKLKS 1450
MLNFVERNKA WLTVVSAVTS AIGILLLVTK IFKKEESKDE RAYNPTLPVA 1500
KPKGTFPVSQ REFKNEAPYD GQLEHIISQM AYITGSTTGH ITHCAGYQHD 1550
EIILHGHSIK YLEQEEELTL HYKNKVFPIE QPSVTQVTLG GKPMDLAIVK 1600
CKLPFRFKKN SKYYTNKIGT ESMLIWMTEQ GIITKEVQRV HHSGGIKTRE 1650
GTESTKTISY TVKSCKGMCG GLLISKVEGN FKILGMHIAG NGEMGVAIPF 1700
NFLKNDMSDQ GIVTEVTPIQ PMYINTKSQI HKSPVYGAVE VKMGPAVLSK 1750
SDTRLEEPVD CLVKKSASKY RVNKFQVNNE LWQGVKACVK SKFREIFGVN 1800
GIVDMKTAIL GTSHVNSMDL STSAGYSFVK SGYKKKDLIC LEPFSVSPML 1850
EKLVQEKFHN LLKGNQITTI FNTCLKDELR KLDKIATGKT RCIEACEIDY 1900
CIVYRMIMME IYDKIYQTPC YYSGLAVGIN PYRDWHFMIN ALNDYNYEMD 1950
YSQYDGSLSS MLLWEAVQVL AYCHDSPDLV MQLHKPVIDS DHVVFNERWL 2000
IHGGMPSGSP CTTVLNSLCN LMMCIYTTNL ISPGIDCLPI VYGDDVILSL 2050
DKEIEPERLQ SIMAESFGAE VTGSRKDEPP SLKPRMEVEF LKRKPGYFPE 2100
STFIVGKLDT ENMIQHLMWM KNFSTFKQQL QSYLMELCLH GKDTYQHYVK 2150
ILNPYLKEWN IPVDDYEVVI GKLVPMVFD 2179
Length:2,179
Mass (Da):245,874
Last modified:August 1, 1998 - v1
Checksum:i96803C0BB8856664
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005695 Genomic RNA. Translation: CAA06679.1.
RefSeqiNP_046804.1. NC_001897.1.

Genome annotation databases

GeneIDi1403455.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AJ005695 Genomic RNA. Translation: CAA06679.1 .
RefSeqi NP_046804.1. NC_001897.1.

3D structure databases

ProteinModelPortali O73556.
ModBasei Search...

Protein family/group databases

MEROPSi C03.023.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 1403455.

Family and domain databases

Gene3Di 2.60.120.20. 2 hits.
3.40.50.300. 1 hit.
InterProi IPR004004. Helic/Pol/Pept_Calicivir-typ.
IPR000605. Helicase_SF3_ssDNA/RNA_vir.
IPR014759. Helicase_SF3_ssRNA_vir.
IPR027417. P-loop_NTPase.
IPR000199. Peptidase_C3A/C3B_picornavir.
IPR001676. Picornavirus_capsid.
IPR001205. RNA-dir_pol_C.
IPR007094. RNA-dir_pol_PSvirus.
IPR009003. Trypsin-like_Pept_dom.
IPR029053. Viral_coat.
IPR009419. VPP_parechovir_P3A.
IPR009407. VPP_parechovir_P3B.
[Graphical view ]
Pfami PF06344. Parecho_VpG. 1 hit.
PF00548. Peptidase_C3. 1 hit.
PF06363. Picorna_P3A. 1 hit.
PF00680. RdRP_1. 1 hit.
PF00073. Rhv. 1 hit.
PF00910. RNA_helicase. 1 hit.
[Graphical view ]
PRINTSi PR00918. CALICVIRUSNS.
SUPFAMi SSF50494. SSF50494. 1 hit.
SSF52540. SSF52540. 1 hit.
PROSITEi PS50507. RDRP_SSRNA_POS. 1 hit.
PS51218. SF3_HELICASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular analysis of human parechovirus type 2 (formerly echovirus 23)."
    Ghazi F., Hughes P.J., Hyypiae T., Stanway G.
    J. Gen. Virol. 79:2641-2650(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Entry informationi

Entry nameiPOLG_HPE2W
AccessioniPrimary (citable) accession number: O73556
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi