O72904 (NPH2_FOWPN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA helicase NPH-II
EC=3.6.4.13

Alternative name(s):
Nucleoside triphosphatase II
Short name=NTPase II
Nucleoside triphosphate phosphohydrolase II
Short name=NPH II

Gene names

Name:	NPH2
Ordered Locus Names:	FPV082
ORF Names:	FPI8R

Organism

Fowlpox virus (strain NVSL) (FPV)

Taxonomic identifier

928301 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Poxviridae › Chordopoxvirinae › Avipoxvirus

Virus host

Vertebrata [TaxID: 7742]

Protein attributes

Sequence length	682 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	NTP-dependent helicase that catalyzes unidirectional unwinding of 3'tailed duplex RNAs and plays an important role during transcription of early mRNAs, presumably by preventing R-loop formation behind the elongating RNA polymerase. Might also play a role in the export of newly synthesized mRNA chains out of the core into the cytoplasm. Required for replication and propagation of viral particles By similarity.
Catalytic activity	ATP + H2O = ADP + phosphate.
Subunit structure	Monomer.
Subcellular location	Virion. Note: Localizes to the virion core By similarity.
Sequence similarities	Belongs to the DEAD box helicase family. DEAH subfamily. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Biological process	Transcription
Cellular component	Virion
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ATP-dependent helicase activity Inferred from electronic annotation. Source: InterPro nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 682	682	RNA helicase NPH-II		PRO_0000055187
Regions
	Domain	181 – 354	174	Helicase ATP-binding
	Domain	386 – 551	166	Helicase C-terminal
	Nucleotide binding	194 – 201	8	ATP By similarity
	Motif	303 – 306	4	DEXH box

Sequences

	Sequence		Length	Mass (Da)
	O72904 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: F394E69FD9745652	FASTA	682	79,808
	10 20 30 40 50 60 MTTNDLFSIY AFSNMYDIFP RKYSQKELEE YHRKNPLFFS YTIFPVIKHR WSKAYICFQN 70 80 90 100 110 120 NVYMLNIELD TSKPYDRVPI QHLIDIRPIS TDIKKEIYKI SDKTFITFEC YSYLKCKGYN 130 140 150 160 170 180 SIYDITLDDK RGLLSAGNIL SIFSSNKQMP EKSSIGILKN PKPFTVIKFK SLSLITQLQI 190 200 210 220 230 240 FELLRKRKQI VVTGSTGIGK TSQLPKVIMW YNYLFGGWDN LDRVRFDYIS RPIVLSLPRV 250 260 270 280 290 300 ALVKSNGINF LQSLGFSDFE GSPVELRYGG KTEHTTRQHD GIVLSTNKLT SYSLSNYNII 310 320 330 340 350 360 IVDEIHEHDR IADIIISVLR KNIDTIHSLV LMSATLEDDR DRLQEFLPDV EFYHIEGPVL 370 380 390 400 410 420 YSIKEIYVKN KYSYDSKAYT EEEKKNISTT LNWCRPRNGM CGILFLASVS QCISYKKYLE 430 440 450 460 470 480 KSNSDMDFII IHGKIPDITE VLNAVQRPGR ERPCILVSTP YLESSITIRT ATHVYDTGRV 490 500 510 520 530 540 YVPKPFGGDQ LFISKSMMTQ RKGRVGRVSK GIYVYFYDMC LLKPIKSIDH EFLYEYIVYA 550 560 570 580 590 600 KKFKLSLPND LLVIPSDKDM LKKSEEYIKS FNISFDRLFE IYVNYFVNMV EYVKIYNKGG 610 620 630 640 650 660 KKAENLDMFE RNDILTGETL KDIKNLQLLV KINTTTRRKK MYCYKGEILF GPYMNTVIRL 670 680 SSKQLYRNYV YMLTERSFTL YR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Gene translocations in poxviruses: the fowlpox virus thymidine kinase gene is flanked by 15 bp direct repeats and occupies the locus which in vaccinia virus is occupied by the ribonucleotide reductase large subunit gene." Binns M.M., Boursnell M.E.G., Skinner M.A. Virus Res. 24:161-172(1992) [PubMed: 1326827] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: FP-9 / Isolate HP-440.
[2]	"The genome of fowlpox virus." Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L. J. Virol. 74:3815-3831(2000) [PubMed: 10729156] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Web resources

The DExH/D protein family database

Poxviridae DExH proteins

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ223385 Genomic DNA. Translation: CAA11299.1. AF198100 Genomic DNA. Translation: AAF44426.1.
PIR	G48563.
RefSeq	NP_039045.1. NC_002188.1.
3D structure databases
ProteinModelPortal	O72904.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1486630.
Phylogenomic databases
ProtClustDB	PHA2653.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR011545. DNA/RNA_helicase_DEAD/DEAH_N. IPR001650. Helicase_C. IPR021892. NPH-II. [Graphical view]
Pfam	PF00270. DEAD. 1 hit. PF12011. DUF3503. 1 hit. PF00271. Helicase_C. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS00690. DEAH_ATP_HELICASE. False negative. PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NPH2_FOWPN

Accession

Primary (citable) accession number: O72904

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program