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Protein

Replication-associated protein

Gene

AC1

Organism
Cotton leaf curl Multan virus-[62]
Status
Unreviewed-Annotation score: -Protein inferred from homologyi

Functioni

Essential for the replication of viral ssDNA. The closed circular ssDNA genome is first converted to a superhelical dsDNA. Rep binds a specific region at the genome origin of replication. It introduces an endonucleolytic nick within the conserved sequence 5'-TAATATTAC-3' in the intergenic region of the genome present in all geminiviruses, thereby initiating the rolling circle replication (RCR). Following cleavage, binds covalently to the 5'-phosphate of DNA as a tyrosyl ester. The cleavage gives rise to a free 3'-OH that serves as a primer for the cellular DNA polymerase. The polymerase synthesizes the (+) strand DNA by rolling circle mechanism. After one round of replication, a Rep-catalyzed nucleotidyl transfer reaction releases a circular single-stranded virus genome, thereby terminating the replication. Displays origin-specific DNA cleavage, nucleotidyl transferase, ATPase and helicase activities.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • Mg2+UniRule annotation
  • Mn2+UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDNA-bindingUniRule annotation, EndonucleaseUniRule annotation, HelicaseUniRule annotation, Hydrolase, Multifunctional enzymeUniRule annotation, Nuclease, NucleotidyltransferaseUniRule annotation, Transferase
LigandATP-binding, Metal-bindingUniRule annotation, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replication-associated proteinUniRule annotation (EC:3.1.21.-UniRule annotation)
Short name:
RepUniRule annotation
Gene namesi
Name:AC1Imported
OrganismiCotton leaf curl Multan virus-[62]Imported
Taxonomic identifieri223254 [NCBI]
Taxonomic lineageiVirusesssDNA virusesGeminiviridaeBegomovirus
Proteomesi
  • UP000202798 Componenti: Genome

Subcellular locationi

  • Host nucleus UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Host nucleusUniRule annotation

PTM / Processingi

Keywords - PTMi

Covalent protein-DNA linkageUniRule annotation

Interactioni

Subunit structurei

Homooligomer.UniRule annotation

Structurei

3D structure databases

ProteinModelPortaliO72705
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 119Gemini_AL1InterPro annotationAdd BLAST113
Domaini126 – 230Gemini_AL1_MInterPro annotationAdd BLAST105

Domaini

There are 3 rolling circle replication (RCR) motifs. RCR-2 is probably involved in metal coordination. RCR-3 is required for phosphodiester bond cleavage for initiation of RCR.UniRule annotation

Sequence similaritiesi

Belongs to the geminiviridae Rep protein family.UniRule annotation

Family and domain databases

InterProiView protein in InterPro
IPR001301 Gemini_AL1_CLV
IPR001191 Gemini_AL1_REP
IPR022690 Gemini_AL1_REP_cat-dom
IPR022692 Gemini_AL1_REP_central
PfamiView protein in Pfam
PF00799 Gemini_AL1, 1 hit
PF08283 Gemini_AL1_M, 1 hit
PRINTSiPR00227 GEMCOATAL1
PR00228 GEMCOATCLVL1

Sequencei

Sequence statusi: Complete.

O72705-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPPKRFKVQ AKNYFITYPQ CSLTKEEALS QIQAINTPSN KKYIKICREL
60 70 80 90 100
HEDGSPHLHV LIQFEGKFVC TNNRFFDLVS PTRSAHFHPN IQGAKSSSDV
110 120 130 140 150
KAYIDKDGDT LEWGEFQIDG RSARGGQQTA NDAYAEALNA GSKSEALRVI
160 170 180 190 200
KELAPKDFVL QFHNLNANLD RIFQEPPAPY ISPFSPSSFD QVPEELEVWA
210 220 230 240 250
IDNVVDPAAR PLRPRSIVIE GDSRTGKTMW ARSLGPHNYL CGHLDLSPKV
260 270 280 290 300
YSNDAWYNVI DDVDPHFLKH FKEFMGAQRD WQSNTKYGKP VQIKGGIPTI
310 320 330 340 350
FLCNPGPNSS YKEFLDEEKN TALKNWAVKN AIFITLEGPL YSGTNQSTAQ
360
GSEETQQEEE SRS
Length:363
Mass (Da):40,886
Last modified:August 1, 1998 - v1
Checksum:i51C17693B47E2E20
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ002447 Genomic DNA Translation: CAA05421.1
RefSeqiNP_803141.1, NC_004607.1

Genome annotation databases

GeneIDi1483026
KEGGivg:1483026

Similar proteinsi

Entry informationi

Entry nameiO72705_9GEMI
AccessioniPrimary (citable) accession number: O72705
Entry historyiIntegrated into UniProtKB/TrEMBL: August 1, 1998
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 58 of the entry and version 1 of the sequence. See complete history.
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported
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Main funding by: National Institutes of Health