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Protein

Replicase polyprotein P2AB

Gene

ORF2A-2B

Organism
Southern bean mosaic virus (isolate Bean/United States/Arkansas) (SBMV)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease: responsible for cleavages of polyprotein P2A and replicase polyprotein P2AB.
VPg: covalently attached to the 5' extremity of the genomic and subgenomic RNAs. It may serve as a primer for the replicase.
RNA-directed RNA polymerase: replicates the viral genome.Curated

Catalytic activityi

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei181For protease activitySequence analysis1
Active sitei216For protease activitySequence analysis1
Active sitei284For protease activitySequence analysis1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Nucleotidyltransferase, Protease, RNA-directed RNA polymerase, Serine protease, Transferase

Keywords - Biological processi

Viral RNA replication

Keywords - Ligandi

Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Replicase polyprotein P2AB
Cleaved into the following 4 chains:
Gene namesi
ORF Names:ORF2A-2B
OrganismiSouthern bean mosaic virus (isolate Bean/United States/Arkansas) (SBMV)
Taxonomic identifieri652938 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA positive-strand viruses, no DNA stageSobemovirus
Virus hostiGlycine max (Soybean) (Glycine hispida) [TaxID: 3847]
Phaseolus vulgaris (Kidney bean) (French bean) [TaxID: 3885]
Vigna mungo (Black gram) (Phaseolus mungo) [TaxID: 3915]
Vigna unguiculata (Cowpea) [TaxID: 3917]
Proteomesi
  • UP000001671 Componenti: Genome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei10 – 30HelicalSequence analysisAdd BLAST21
Transmembranei36 – 56HelicalSequence analysisAdd BLAST21
Transmembranei74 – 96HelicalSequence analysisAdd BLAST23

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004024701 – 962Replicase polyprotein P2ABAdd BLAST962
ChainiPRO_00004098471 – 132N-terminal proteinSequence analysisAdd BLAST132
ChainiPRO_0000409848133 – 325Serine proteaseSequence analysisAdd BLAST193
ChainiPRO_0000409849326 – 402VPgSequence analysisAdd BLAST77
ChainiPRO_0000409850403 – 962RNA-directed RNA polymeraseSequence analysisAdd BLAST560

Post-translational modificationi

The polyprotein is proteolytically cleaved into several chains by the viral protease.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei132 – 133Cleavage; by viral serine proteaseSequence analysis2
Sitei325 – 326Cleavage; by viral serine proteaseSequence analysis2
Sitei402 – 403Cleavage; by viral serine proteaseSequence analysis2

Keywords - PTMi

Covalent protein-RNA linkage

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini166 – 301Peptidase S39AAdd BLAST136
Domaini692 – 806RdRp catalyticAdd BLAST115

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi378 – 382Poly-Asp5
Compositional biasi385 – 388Poly-Pro4

Sequence similaritiesi

Contains 1 peptidase S39A domain.Curated
Contains 1 RdRp catalytic domain.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR000382. Peptidase_S39B_luteovirus.
IPR001795. RNA-dir_pol_luteovirus.
[Graphical view]
PfamiPF02122. Peptidase_S39. 1 hit.
PF02123. RdRP_4. 1 hit.
[Graphical view]
PRINTSiPR00914. LVIRUSRNAPOL.
SUPFAMiSSF50494. SSF50494. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by ribosomal frameshifting. AlignAdd to basket

Isoform Replicase polyprotein P2AB (identifier: O72157-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MYHPGRSPSF LITLANVICA AILFDIHTGG YQPGSLIPIV AWMTPFVTLL
60 70 80 90 100
WLSASFATYL YKYVRTRLLP EEKVARVYYT AQSAPYFDPA LGVMMQFAPS
110 120 130 140 150
HGGASIEVQV NPSWISLLGG SLKINGDDAS NESAVLGSFY SSVKPGDEPA
160 170 180 190 200
SLVAIKSGPQ TIGFGCRTKI DGDDCLFTAN HVWNNSMRPT ALAKRGKQVA
210 220 230 240 250
IEDWETPLSC DHKMLDFVVV RVPKHVWSKL GVKATQLVCP SDKDAVTCYG
260 270 280 290 300
GSSSDNLLSG TGVCSKVDFS WKLTHSCPTA AGWSGTPIYS SRGVVGMHVG
310 320 330 340 350
FEDIGKLNRG VNAFYVSNYL LRSQETLPPE LSVIEIPFED VETRSYEFIE
360 370 380 390 400
VEIKGRGKAK LGKREFAWIP ESGKYWADDD DDSLPPPPKV VDGKMVWSSA
410 420 430 440 450
QETVAEPLNL PAGGRVKALA ALSQLAGYDF KEGEAASTRG MPLRFVGQSA
460 470 480 490 500
CKFRELCRKD TPDEVLRATR VFPELSDFSW PERGSKAELH SLLLQAGKFN
510 520 530 540 550
PTGIPRNLEG ACQNLLERYP ASKSCYCLRG EAWSFDAVYE EVCKKAQSAE
560 570 580 590 600
INEKASPGVP LSRLASTNKD LLKRHLELVA LCVTERLFLL SEAEDLLDES
610 620 630 640 650
PVDLVRRGLC DPVRLFVKQE PHASRKVREG RFRLISSVSL VDQLVERMLF
660 670 680 690 700
GPQNQLEIAE WEHIPSKPGM GLSLRQQAKS LFDDLRVKHS RCPAAEADIS
710 720 730 740 750
GFDWSVQDWE LWADVEMRIV LGGFGHKLAK AAQNRFSCFM NSVFQLSDGT
760 770 780 790 800
LIEQQLPGIM KSGSYCTSST NSRIRCLMAE LIGSPWCIAM GDDSVEGWVD
810 820 830 840 850
GAKDKYMRLG HTCKDYKPCA TTISGRLYEV EFCSHVIRED RCWLASWPKT
860 870 880 890 900
LFKYLSEGKW FFEDLERDVS SSPHWPRIRH YVVGNTPSPH KTNLQNQSPR
910 920 930 940 950
YGEEVDKTTV NQGYSEHSGS PGHSIEEAQE PEAAPFCCEA ASVYPGWGVH
960
GPYCSGDYGS LT
Note: Produced by -1 ribosomal frameshifting at the 2A-2B genes boundary.
Length:962
Mass (Da):106,566
Last modified:June 28, 2011 - v2
Checksum:iBF840BC106F9CF82
GO
Isoform Polyprotein P2A (identifier: O73564-1) [UniParc]FASTAAdd to basket
The sequence of this isoform can be found in the external entry O73564.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.
Note: Produced by conventional translation.
Length:575
Mass (Da):62,722
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055887 Genomic RNA. Translation: AAC15983.1.

Keywords - Coding sequence diversityi

Ribosomal frameshifting

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055887 Genomic RNA. Translation: AAC15983.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR009003. Peptidase_S1_PA.
IPR000382. Peptidase_S39B_luteovirus.
IPR001795. RNA-dir_pol_luteovirus.
[Graphical view]
PfamiPF02122. Peptidase_S39. 1 hit.
PF02123. RdRP_4. 1 hit.
[Graphical view]
PRINTSiPR00914. LVIRUSRNAPOL.
SUPFAMiSSF50494. SSF50494. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRDRP_SBMVA
AccessioniPrimary (citable) accession number: O72157
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 30, 2010
Last sequence update: June 28, 2011
Last modified: September 16, 2015
This is version 80 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.