O72120 (CAPSD_CACV4) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Capsid protein

Alternative name(s):
Coat protein
Short name=CP
VP1

Gene names

ORF Names:

ORF2

Organism

Canine calicivirus (strain 48) (CaCV)

Taxonomic identifier

292348 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Caliciviridae › unclassified Caliciviridae › Canine calicivirus

Virus host

Canis familiaris (Dog) (Canis lupus familiaris) [TaxID: 9615]

Protein attributes

Sequence length	691 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Capsid protein self assembles to form an icosahedral capsid with a T=3 symmetry, about 38 nm in diameter, and consisting of 180 capsid proteins. A smaller form of capsid with a diameter of 23 nm might be capsid proteins assembled as icosahedron with T=1 symmetry. The capsid encapsulate the genomic RNA and VP2 proteins. Attaches virion to target cells by binding to specific cellular receptor. Once attached, the virion is endocytosed. Acidification of the endosome induces conformational change of capsid protein thereby injecting virus genomic RNA into host cytoplasm By similarity.
Subunit structure	Homodimerizes, then multimerizes. May bind to VP3 and Vpg proteins By similarity.
Subcellular location	Virion. Host cytoplasm By similarity.
Post-translational modification	Cleaved by virus calcivirin to produce mature capsid protein By similarity.
Sequence similarities	Belongs to the caliciviridae capsid protein family.

Ontologies

Keywords
Cellular component	Host cytoplasm Virion
Molecular function	Capsid protein T=3 icosahedral capsid protein
Gene Ontology (GO)
Cellular_component	T=3 icosahedral viral capsid Inferred from electronic annotation. Source: UniProtKB-KW host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Propeptide	1 – 157	157			PRO_0000036872
	Chain	158 – 691	534	Capsid protein		PRO_0000036873
Sites
	Site	157 – 158	2	Cleavage; by 3C-like protease
Experimental info
	Mutagenesis	157	1	E → K: Complete loss of processing by the viral protease. Ref.3
	Mutagenesis	158	1	S → P: Complete loss of processing by the viral protease. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	O72120 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: F1C9774C9217AEF4	FASTA	691	76,181
	10 20 30 40 50 60 MARYLELNPQ NYSDEEYDYD SYNPFPNFEK NLASHYGTDF VPRINLDDFF LDDEDFEFCD 70 80 90 100 110 120 DPLNCCFPDY LASLGEEEFI YEGDEPYIVL KHQLVSSTMW DDGTFTYPIL PPFKTSSISY 130 140 150 160 170 180 FLPKPGEVLH RCLMAVAKGM DPDLQVAVGT EFQFRAESDS SHPPDITTED QGTVVATGPQ 190 200 210 220 230 240 PSAPAMATLA TAATGTMPEE WKNFFSYYTT INWATTDETG KVLFVQNLAP RMNPFLDHIA 250 260 270 280 290 300 KMYTGWSGSM EVRFTISGSG VFGGKVAAVL VPPGISTEGG TNLLQFPHVL VDARQTEPVI 310 320 330 340 350 360 FTIPDIRTQL WHDMHDTSTS HLVILVYNDL VNPFQGGENG TSCTITVETR GGTDFEFHLL 370 380 390 400 410 420 KPPTRKMIFG ADPSRLIPRR SQFWEGNRLP GVITSFVCLP RMFQANRHFD CKRQTFGWSR 430 440 450 460 470 480 PVHKGIEVRV DATNKDAANT TDIGIHVVTA RNAIKSDIPD GWPDYYRTGE QVYNNTTQTF 490 500 510 520 530 540 QEVKESVMGS AVPDSTATAM TWHHLPTVVF GHGTAVGSKT TNSKVLSGNF YAIGNFDQSG 550 560 570 580 590 600 NIKLYPSYWI AKEQSAGGAP IGAYEDMVKR IDVLPTAQTT GGNFPVAFVS KFASSHNGNG 610 620 630 640 650 660 VSVYNSQILT TSALLAQDVY DIGPNALAVF KIKGSGGYWF DLGISADGFS YVGGGNLNFS 670 680 690 SLQFPLEATY VGMASLHNKL QYNLGGSATT L

References

[1]	"Organization of the canine calicivirus genome from the RNA polymerase gene to the poly(A) tail." Roerink F., Hashimoto M., Tohya Y., Mochizuki M. J. Gen. Virol. 80:929-935(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Complete nucleotide sequence, genome organization and phylogenic analysis of the canine calicivirus." Matsuura Y., Tohya Y., Nakamura K., Shimojima M., Roerink F., Mochizuki M., Takase K., Akashi H., Sugimura T. Virus Genes 25:67-73(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[3]	"Expression and processing of the canine calicivirus capsid precursor." Matsuura Y., Tohya Y., Onuma M., Roerink F., Mochizuki M., Sugimura T. J. Gen. Virol. 81:195-199(2000) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEOLYTIC PROCESSING, MUTAGENESIS OF GLU-157 AND SER-158.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF053720 Genomic RNA. Translation: AAC16446.1. AB070225 Genomic RNA. Translation: BAB83602.1.
RefSeq	NP_777374.1. NC_004542.1.
3D structure databases
ProteinModelPortal	O72120.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR004005. Calicivirus_coat. [Graphical view]
Pfam	PF00915. Calici_coat. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CAPSD_CACV4

Accession

Primary (citable) accession number: O72120

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 9, 2004
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program