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Protein

Large envelope protein

Gene

S

Organism
Woolly monkey hepatitis B virus (isolate Louisville) (WMHBV)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Protein inferred from homologyi

Functioni

The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assures fusion between virion membrane and endosomal membrane. In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.UniRule annotation
The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid.UniRule annotation

GO - Biological processi

Keywordsi

Biological processCaveolin-mediated endocytosis of virus by host, Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus endocytosis by host, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Large envelope proteinUniRule annotation
Alternative name(s):
L glycoproteinUniRule annotation
L-HBsAgUniRule annotation
Short name:
LHBUniRule annotation
Large S proteinUniRule annotation
Large surface proteinUniRule annotation
Major surface antigenUniRule annotation
Gene namesi
Name:SUniRule annotation
OrganismiWoolly monkey hepatitis B virus (isolate Louisville) (WMHBV)
Taxonomic identifieri490134 [NCBI]
Taxonomic lineageiVirusesRetro-transcribing virusesHepadnaviridaeOrthohepadnavirus
Virus hostiLagothrix lagotricha (Brown woolly monkey) (Humboldt's woolly monkey) [TaxID: 9519]
Proteomesi
  • UP000008599 Componenti: Genome
  • UP000163759 Componenti: Genome

Subcellular locationi

O71305:
  • Virion membrane UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 242Intravirion; in internal conformationUniRule annotationAdd BLAST241
Topological domaini2 – 170Virion surface; in external conformationUniRule annotationAdd BLAST169
Transmembranei171 – 191Helical; Name=TM1; Note=In external conformationUniRule annotationAdd BLAST21
Topological domaini192 – 242Intravirion; in external conformationUniRule annotationAdd BLAST51
Transmembranei243 – 263Helical; Name=TM2UniRule annotationAdd BLAST21
Topological domaini264 – 339Virion surfaceUniRule annotationAdd BLAST76
Transmembranei340 – 360HelicalUniRule annotationAdd BLAST21
Topological domaini361 – 366IntravirionUniRule annotation6
Transmembranei367 – 389Helical; Name=TM3UniRule annotationAdd BLAST23
Topological domaini390 – 391Virion surfaceUniRule annotation2

GO - Cellular componenti

Keywords - Cellular componenti

Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved; by hostUniRule annotation
ChainiPRO_00003192942 – 391Large envelope proteinUniRule annotationAdd BLAST390

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi2N-myristoyl glycine; by hostUniRule annotation1
Glycosylationi311N-linked (GlcNAc...) asparagine; by hostUniRule annotation1
Isoform M (identifier: O71305-2)
Modified residuei1N-acetylmethionineBy similarity1

Post-translational modificationi

Isoform M is N-terminally acetylated by host at a ratio of 90%, and N-glycosylated by host at the pre-S2 region.UniRule annotationBy similarity
Myristoylated.UniRule annotation

Keywords - PTMi

Acetylation, Glycoprotein, Lipoprotein, Myristate

Interactioni

Subunit structurei

Li-HBsAg interacts with capsid protein and with HDV Large delta antigen. Isoform M associates with host chaperone CANX through its pre-S2 N glycan. This association may be essential for M proper secretion.UniRule annotation

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 163Pre-SUniRule annotationAdd BLAST162
Regioni2 – 108Pre-S1UniRule annotationAdd BLAST107
Regioni109 – 163Pre-S2UniRule annotationAdd BLAST55

Domaini

The large envelope protein is synthesized with the pre-S region at the cytosolic side of the endoplasmic reticulum and, hence will be within the virion after budding. Therefore the pre-S region is not N-glycosylated. Later a post-translational translocation of N-terminal pre-S and TM1 domains occur in about 50% of proteins at the virion surface. These molecules change their topology by an unknown mechanism, resulting in exposure of pre-S region at virion surface. For isoform M in contrast, the pre-S2 region is translocated cotranslationally to the endoplasmic reticulum lumen and is N-glycosylated.UniRule annotation

Sequence similaritiesi

Belongs to the orthohepadnavirus major surface antigen family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

OrthoDBiVOG090000AH.

Family and domain databases

HAMAPiMF_04075. HBV_HBSAG. 1 hit.
InterProiView protein in InterPro
IPR000349. Hepvir_surfAg.
PfamiView protein in Pfam
PF00695. vMSA. 1 hit.

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing and alternative initiation. AlignAdd to basket

Isoform L (identifier: O71305-1) [UniParc]FASTAAdd to basket
Also known as: Large envelope protein, LHB, L-HBsAg

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGLNQSTFNP LGFFPSHQLD PLFKANAGSA DWDKNPNKDP WPQAHDTAVG
60 70 80 90 100
AFGPGLVPPH GGLLGWSSQA QGLSVTVPDT PPPPSTNRDK GRKPTPATPP
110 120 130 140 150
LRDTHPQAMT WNTSSFQSYL QNPKVRGLYF PAGGSTSSIV NPVPTTASTT
160 170 180 190 200
SSSFSTTGVP VSTMDITSSG FLGPLLALQA VFFLLTKILT MPQSLDSLWT
210 220 230 240 250
SLNFLGGTPA CPGLNSQSPT SSHSPTCCPP TCPGYRWMCL RRSIIFLFIL
260 270 280 290 300
LLCLIFLLVL LDYQGMLPVC PLLPTVTGTT TTTGPCRTCT PIVPGISSYP
310 320 330 340 350
SCCCTKPTDG NCTCIPIPSS WAFAKFLWDW ALARFSWLNS LLPFVQWFAG
360 370 380 390
LSPTVWLLVI WMMWFWGPSL FSILSPFLPL LPLFFWLWAY I
Length:391
Mass (Da):42,557
Last modified:August 1, 1998 - v1
Checksum:iBB69E0686FBA1E9D
GO
Isoform M (identifier: O71305-2) [UniParc]FASTAAdd to basket
Also known as: Middle envelope protein, MHB, M-HBsAg

The sequence of this isoform differs from the canonical sequence as follows:
     1-108: Missing.

Show »
Length:283
Mass (Da):31,154
Checksum:i599C28D8E866454F
GO
Isoform S (identifier: O71305-3) [UniParc]FASTAAdd to basket
Also known as: Small envelope protein, SHB, S-HBsAg

The sequence of this isoform differs from the canonical sequence as follows:
     1-163: Missing.

Show »
Length:228
Mass (Da):25,438
Checksum:iFF8FF77A89A0C487
GO

Sequence cautioni

O71305: The sequence AAC16906 differs from that shown. Reason: Erroneous initiation.Curated
O71305: The sequence AAO74857 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0314701 – 163Missing in isoform S. CuratedAdd BLAST163
Alternative sequenceiVSP_0314711 – 108Missing in isoform M. CuratedAdd BLAST108

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF046996 Genomic DNA. Translation: AAC16905.1.
AF046996 Genomic DNA. Translation: AAC16906.1. Different initiation.
AY226578 Genomic DNA. Translation: AAO74856.1.
AY226578 Genomic DNA. Translation: AAO74857.1. Different initiation.
RefSeqiYP_009175035.1. NC_028129.1.
YP_009175036.1. NC_028129.1.

Genome annotation databases

GeneIDi26101581.
26101584.
KEGGivg:26101581.
vg:26101584.

Keywords - Coding sequence diversityi

Alternative initiation, Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHBSAG_WMHBV
AccessioniPrimary (citable) accession number: O71305
Secondary accession number(s): O92934
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 1, 1998
Last modified: October 25, 2017
This is version 64 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families