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O71028 (DUT_SIRV1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 68. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable deoxyuridine 5'-triphosphate nucleotidohydrolase

Short name=dUTPase
EC=3.6.1.23
Alternative name(s):
dUTP pyrophosphatase
Gene names
ORF Names:158a
OrganismSulfolobus islandicus rod-shaped virus 1 (SIRV-1) (Sulfolobus virus SIRV-1) [Reference proteome]
Taxonomic identifier157898 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageRudiviridaeRudivirus
Virus hostSulfolobus islandicus [TaxID: 43080]

Protein attributes

Sequence length158 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. It does probably not deaminate dCTP. Ref.1

Catalytic activity

dUTP + H2O = dUMP + diphosphate. Ref.1

Cofactor

Magnesium. Ref.1

Pathway

Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2. HAMAP-Rule MF_00146

Sequence similarities

Belongs to the dUTPase family.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 158158Probable deoxyuridine 5'-triphosphate nucleotidohydrolase HAMAP-Rule MF_00146
PRO_0000342288

Natural variations

Natural variant101T → R in strain: Isolate variant XX.
Natural variant151K → N in strain: Isolate variant XX.
Natural variant201N → D in strain: Isolate variant XX.
Natural variant1331R → H in strain: Isolate variant XX.
Natural variant1481K → N in strain: Isolate variant XX.

Sequences

Sequence LengthMass (Da)Tools
O71028 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 5BA47765E0889190

FASTA15817,906
        10         20         30         40         50         60 
MILSDRDIKT YINSKKLVIN PLSEDTIREN GVDLKIGNEI VRIKENMEKE VGDEFIIYPN 

        70         80         90        100        110        120 
EHVLLTTKEY IKLSNDIIAF CNLRSTFARK GLLIPPTIVD AGFEGQLTIE LVGSSIPVKL 

       130        140        150 
KSGERFLHLI FARTLTPVEK PYNGKYQKQK GVTLAKED 

« Hide

References

« Hide 'large scale' references
[1]"Biochemical and phylogenetic characterization of the dUTPase from the archaeal virus SIRV."
Prangishvili D., Klenk H.-P., Jakobs G., Schmiechen A., Hanselmann C., Holz I., Zillig W.
J. Biol. Chem. 273:6024-6029(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, COFACTOR, ENZYME ACTIVITY.
[2]"Sequences and replication of genomes of the archaeal rudiviruses SIRV1 and SIRV2: relationships to the archaeal lipothrixvirus SIFV and some eukaryal viruses."
Peng X., Blum H., She Q., Mallok S., Bruegger K., Garrett R.A., Zillig W., Prangishvili D.
Virology 291:226-234(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate variant VIII.
[3]"Multiple variants of the archaeal DNA rudivirus SIRV1 in a single host and a novel mechanism of genomic variation."
Peng X., Kessler A., Phan H., Garrett R.A., Prangishvili D.
Mol. Microbiol. 54:366-375(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Isolate variant XX.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF022221 Genomic DNA. Translation: AAC15873.1.
AJ414696 Genomic DNA. Translation: CAC93972.1.
AJ748296 Genomic DNA. Translation: CAG38836.1.
RefSeqNP_666605.1. NC_004087.1.

3D structure databases

ProteinModelPortalO71028.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID951366.

Enzyme and pathway databases

UniPathwayUPA00610; UER00665.

Family and domain databases

Gene3D2.70.40.10. 1 hit.
HAMAPMF_00146. dCTP_deaminase.
InterProIPR011962. dCTP_deam.
IPR029054. dUTPase-like.
IPR008180. dUTPase/dCTP_deaminase.
[Graphical view]
PfamPF00692. dUTPase. 1 hit.
[Graphical view]
SUPFAMSSF51283. SSF51283. 1 hit.
TIGRFAMsTIGR02274. dCTP_deam. 1 hit.
ProtoNetSearch...

Entry information

Entry nameDUT_SIRV1
AccessionPrimary (citable) accession number: O71028
Secondary accession number(s): Q5TJA2, Q777W3
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: August 1, 1998
Last modified: June 11, 2014
This is version 68 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways