O71028 (DUT_SIRV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable deoxyuridine 5'-triphosphate nucleotidohydrolase
Short name=dUTPase
EC=3.6.1.23

Alternative name(s):
dUTP pyrophosphatase

Gene names

ORF Names:

158a

Organism

Sulfolobus islandicus rod-shaped virus 1 (SIRV-1) (Sulfolobus virus SIRV-1) [Reference proteome]

Taxonomic identifier

157898 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Rudiviridae › Rudivirus

Virus host

Sulfolobus islandicus [TaxID: 43080]

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This enzyme is involved in nucleotide metabolism: it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA. It does probably not deaminate dCTP. Ref.1
Catalytic activity	dUTP + H2O = dUMP + diphosphate. Ref.1
Cofactor	Magnesium. Ref.1
Pathway	Pyrimidine metabolism; dUMP biosynthesis; dUMP from dCTP (dUTP route): step 1/2.
Sequence similarities	Belongs to the dUTPase family.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	dUMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway dUTP biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	dCTP deaminase activity Inferred from electronic annotation. Source: InterPro dUTP diphosphatase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 158	158	Probable deoxyuridine 5'-triphosphate nucleotidohydrolase		PRO_0000342288
Natural variations
	Natural variant	10	1	T → R in strain: Isolate variant XX.
	Natural variant	15	1	K → N in strain: Isolate variant XX.
	Natural variant	20	1	N → D in strain: Isolate variant XX.
	Natural variant	133	1	R → H in strain: Isolate variant XX.
	Natural variant	148	1	K → N in strain: Isolate variant XX.

Sequences

	Sequence		Length	Mass (Da)
	O71028 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 5BA47765E0889190	FASTA	158	17,906
	10 20 30 40 50 60 MILSDRDIKT YINSKKLVIN PLSEDTIREN GVDLKIGNEI VRIKENMEKE VGDEFIIYPN 70 80 90 100 110 120 EHVLLTTKEY IKLSNDIIAF CNLRSTFARK GLLIPPTIVD AGFEGQLTIE LVGSSIPVKL 130 140 150 KSGERFLHLI FARTLTPVEK PYNGKYQKQK GVTLAKED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Biochemical and phylogenetic characterization of the dUTPase from the archaeal virus SIRV." Prangishvili D., Klenk H.-P., Jakobs G., Schmiechen A., Hanselmann C., Holz I., Zillig W. J. Biol. Chem. 273:6024-6029(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, COFACTOR, ENZYME ACTIVITY.
[2]	"Sequences and replication of genomes of the archaeal rudiviruses SIRV1 and SIRV2: relationships to the archaeal lipothrixvirus SIFV and some eukaryal viruses." Peng X., Blum H., She Q., Mallok S., Bruegger K., Garrett R.A., Zillig W., Prangishvili D. Virology 291:226-234(2001) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate variant VIII.
[3]	"Multiple variants of the archaeal DNA rudivirus SIRV1 in a single host and a novel mechanism of genomic variation." Peng X., Kessler A., Phan H., Garrett R.A., Prangishvili D. Mol. Microbiol. 54:366-375(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Isolate variant XX.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF022221 Genomic DNA. Translation: AAC15873.1. AJ414696 Genomic DNA. Translation: CAC93972.1. AJ748296 Genomic DNA. Translation: CAG38836.1.
RefSeq	NP_666605.1. NC_004087.1.
3D structure databases
HSSP	HSSP built from PDB template 1PKK based on UniProtKB Q57872.
ProteinModelPortal	O71028.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	951366.
Phylogenomic databases
ProtClustDB	PHA1707.
Enzyme and pathway databases
UniPathway	UPA00610; UER00665.
Family and domain databases
InterPro	IPR011962. dCTP_deam. IPR008180. dUTP_pyroPase. [Graphical view]
Pfam	PF00692. dUTPase. 1 hit. [Graphical view]
TIGRFAMs	TIGR02274. dCTP_deam. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DUT_SIRV1

Accession

Primary (citable) accession number: O71028
Secondary accession number(s): Q5TJA2, Q777W3

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 2008
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program