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O70628 (PDE9A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A

EC=3.1.4.35
Gene names
Name:Pde9a
Synonyms:Pde8b
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length534 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.

Catalytic activity

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactor

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulation

Inhibited by SCH 51866 and moderately, by zaprinast.

Tissue specificity

Highly expressed in kidney. Lower levels in liver, lung and brain.

Domain

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similarities

Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.

Ontologies

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced.
Isoform 1 (identifier: O70628-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 534534High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
PRO_0000198842

Regions

Nucleotide binding251 – 2555cGMP By similarity
Nucleotide binding451 – 4522cGMP By similarity
Region227 – 489263Catalytic By similarity

Sites

Active site2511Proton donor By similarity
Metal binding2551Divalent metal cation 1 By similarity
Metal binding2911Divalent metal cation 1 By similarity
Metal binding2921Divalent metal cation 1 By similarity
Metal binding2921Divalent metal cation 2 By similarity
Metal binding4011Divalent metal cation 1 By similarity
Binding site2921cGMP By similarity
Binding site4011cGMP By similarity
Binding site4231cGMP By similarity

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 28126C7BB7375241

FASTA53461,636
        10         20         30         40         50         60 
MGAGSSSYRP KAIYLDIDGR IQKVVFSKYC NSSDIMDLFC IATGLPRNTT ISLLTTDDAM 

        70         80         90        100        110        120 
VSIDPTMPAN SERTPYKVRP VAVKQVSERE ELIQGVLAQV AEQFSRAFKI NELKAEVANH 

       130        140        150        160        170        180 
LAVLEKRVEL EGLKVVEIEK CKSDIKKMRE ELAARNSRTN CPCKYSFLDN KKLTPRRDVP 

       190        200        210        220        230        240 
TYPKYLLSPE TIEALRKPTF DVWLWEPNEM LSCLEHMYHD LGLVRDFSIN PITLRRWLLC 

       250        260        270        280        290        300 
VHDNYRNNPF HNFRHCFCVT QMMYSMVWLC GLQEKFSQMD ILVLMTAAIC HDLDHPGYNN 

       310        320        330        340        350        360 
TYQINARTEL AVRYNDISPL ENHHCAIAFQ ILARPECNIF ASVPPEGFRQ IRQGMITLIL 

       370        380        390        400        410        420 
ATDMARHAEI MDSFKEKMEN FDYSNEEHLT LLKMILIKCC DISNEVRPME VAEPWVDCLL 

       430        440        450        460        470        480 
EEYFMQSDRE KSEGLPVAPF MDRDKVTKAT AQIGFIKFVL IPMFETVTKL FPVVEETMLR 

       490        500        510        520        530 
PLWESREHYE ELKQLDDAMK ELQKKTESLT SGAPENTTEK NRDAKDSEGH SPPN 

« Hide

References

« Hide 'large scale' references
[1]"Identification and characterization of a novel family of cyclic nucleotide phosphodiesterases."
Soderling S.H., Bayuga S.J., Beavo J.A.
J. Biol. Chem. 273:15553-15558(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Identification and characterization of a novel cyclic nucleotide phosphodiesterase gene (PDE9A) that maps to 21q22.3: alternative splicing of mRNA transcripts, genomic structure and sequence."
Guipponi M., Scott H.S., Kudoh J., Kawasaki K., Shibuya K., Shintani A., Asakawa S., Chen H., Lalioti M.D., Rossier C., Minoshima S., Shimizu N., Antonarakis S.E.
Hum. Genet. 103:386-392(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF031147 mRNA. Translation: AAC24344.1.
AF068247 mRNA. Translation: AAC23996.1.
BC061163 mRNA. Translation: AAH61163.1.
CCDSCCDS28605.1. [O70628-1]
RefSeqNP_001157220.1. NM_001163748.1. [O70628-1]
NP_032830.3. NM_008804.4. [O70628-1]
UniGeneMm.10812.

3D structure databases

ProteinModelPortalO70628.
SMRO70628. Positions 181-504.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteO70628.

Proteomic databases

PRIDEO70628.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000047168; ENSMUSP00000038005; ENSMUSG00000041119. [O70628-1]
ENSMUST00000127929; ENSMUSP00000117611; ENSMUSG00000041119. [O70628-1]
GeneID18585.
KEGGmmu:18585.
UCSCuc008buz.2. mouse. [O70628-1]

Organism-specific databases

CTD5152.
MGIMGI:1277179. Pde9a.

Phylogenomic databases

eggNOGNOG268427.
GeneTreeENSGT00730000110321.
HOGENOMHOG000008058.
HOVERGENHBG053545.
InParanoidO70628.
KOK13761.
OMAPRAFKIN.
OrthoDBEOG7DFXC4.
PhylomeDBO70628.
TreeFamTF314638.

Enzyme and pathway databases

BRENDA3.1.4.35. 3474.

Gene expression databases

ArrayExpressO70628.
BgeeO70628.
CleanExMM_PDE9A.
GenevestigatorO70628.

Family and domain databases

Gene3D1.10.1300.10. 1 hit.
InterProIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSPR00387. PDIESTERASE1.
SMARTSM00471. HDc. 1 hit.
[Graphical view]
PROSITEPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSPDE9A. mouse.
NextBio294460.
PROO70628.
SOURCESearch...

Entry information

Entry namePDE9A_MOUSE
AccessionPrimary (citable) accession number: O70628
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot