Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

O70628

- PDE9A_MOUSE

UniProt

O70628 - PDE9A_MOUSE

Protein

High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A

Gene

Pde9a

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.

    Catalytic activityi

    Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

    Cofactori

    Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.By similarity

    Enzyme regulationi

    Inhibited by SCH 51866 and moderately, by zaprinast.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei251 – 2511Proton donorBy similarity
    Metal bindingi255 – 2551Divalent metal cation 1By similarity
    Metal bindingi291 – 2911Divalent metal cation 1By similarity
    Metal bindingi292 – 2921Divalent metal cation 1By similarity
    Metal bindingi292 – 2921Divalent metal cation 2By similarity
    Binding sitei292 – 2921cGMPBy similarity
    Metal bindingi401 – 4011Divalent metal cation 1By similarity
    Binding sitei401 – 4011cGMPBy similarity
    Binding sitei423 – 4231cGMPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi251 – 2555cGMPBy similarity
    Nucleotide bindingi451 – 4522cGMPBy similarity

    GO - Molecular functioni

    1. 3',5'-cyclic-GMP phosphodiesterase activity Source: MGI
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. cGMP-mediated signaling Source: Ensembl
    2. metabolic process Source: GOC

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    cGMP, Manganese, Metal-binding

    Enzyme and pathway databases

    BRENDAi3.1.4.35. 3474.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A (EC:3.1.4.35)
    Gene namesi
    Name:Pde9a
    Synonyms:Pde8b
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1277179. Pde9a.

    Subcellular locationi

    GO - Cellular componenti

    1. cytosol Source: Ensembl
    2. perikaryon Source: Ensembl

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 534534High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9APRO_0000198842Add
    BLAST

    Proteomic databases

    PRIDEiO70628.

    PTM databases

    PhosphoSiteiO70628.

    Expressioni

    Tissue specificityi

    Highly expressed in kidney. Lower levels in liver, lung and brain.

    Gene expression databases

    ArrayExpressiO70628.
    BgeeiO70628.
    CleanExiMM_PDE9A.
    GenevestigatoriO70628.

    Structurei

    3D structure databases

    ProteinModelPortaliO70628.
    SMRiO70628. Positions 181-504.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni227 – 489263CatalyticBy similarityAdd
    BLAST

    Domaini

    Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiNOG268427.
    GeneTreeiENSGT00730000110321.
    HOGENOMiHOG000008058.
    HOVERGENiHBG053545.
    InParanoidiO70628.
    KOiK13761.
    OMAiPRAFKIN.
    OrthoDBiEOG7DFXC4.
    PhylomeDBiO70628.
    TreeFamiTF314638.

    Family and domain databases

    Gene3Di1.10.1300.10. 1 hit.
    InterProiIPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view]
    PfamiPF00233. PDEase_I. 1 hit.
    [Graphical view]
    PRINTSiPR00387. PDIESTERASE1.
    SMARTiSM00471. HDc. 1 hit.
    [Graphical view]
    PROSITEiPS00126. PDEASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    This entry describes 1 isoform i produced by alternative splicing. Align

    Note: A number of isoforms are produced.

    Isoform 1 (identifier: O70628-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MGAGSSSYRP KAIYLDIDGR IQKVVFSKYC NSSDIMDLFC IATGLPRNTT    50
    ISLLTTDDAM VSIDPTMPAN SERTPYKVRP VAVKQVSERE ELIQGVLAQV 100
    AEQFSRAFKI NELKAEVANH LAVLEKRVEL EGLKVVEIEK CKSDIKKMRE 150
    ELAARNSRTN CPCKYSFLDN KKLTPRRDVP TYPKYLLSPE TIEALRKPTF 200
    DVWLWEPNEM LSCLEHMYHD LGLVRDFSIN PITLRRWLLC VHDNYRNNPF 250
    HNFRHCFCVT QMMYSMVWLC GLQEKFSQMD ILVLMTAAIC HDLDHPGYNN 300
    TYQINARTEL AVRYNDISPL ENHHCAIAFQ ILARPECNIF ASVPPEGFRQ 350
    IRQGMITLIL ATDMARHAEI MDSFKEKMEN FDYSNEEHLT LLKMILIKCC 400
    DISNEVRPME VAEPWVDCLL EEYFMQSDRE KSEGLPVAPF MDRDKVTKAT 450
    AQIGFIKFVL IPMFETVTKL FPVVEETMLR PLWESREHYE ELKQLDDAMK 500
    ELQKKTESLT SGAPENTTEK NRDAKDSEGH SPPN 534
    Length:534
    Mass (Da):61,636
    Last modified:August 1, 1998 - v1
    Checksum:i28126C7BB7375241
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031147 mRNA. Translation: AAC24344.1.
    AF068247 mRNA. Translation: AAC23996.1.
    BC061163 mRNA. Translation: AAH61163.1.
    CCDSiCCDS28605.1. [O70628-1]
    RefSeqiNP_001157220.1. NM_001163748.1. [O70628-1]
    NP_032830.3. NM_008804.4. [O70628-1]
    UniGeneiMm.10812.

    Genome annotation databases

    EnsembliENSMUST00000047168; ENSMUSP00000038005; ENSMUSG00000041119. [O70628-1]
    ENSMUST00000127929; ENSMUSP00000117611; ENSMUSG00000041119. [O70628-1]
    GeneIDi18585.
    KEGGimmu:18585.
    UCSCiuc008buz.2. mouse. [O70628-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF031147 mRNA. Translation: AAC24344.1 .
    AF068247 mRNA. Translation: AAC23996.1 .
    BC061163 mRNA. Translation: AAH61163.1 .
    CCDSi CCDS28605.1. [O70628-1 ]
    RefSeqi NP_001157220.1. NM_001163748.1. [O70628-1 ]
    NP_032830.3. NM_008804.4. [O70628-1 ]
    UniGenei Mm.10812.

    3D structure databases

    ProteinModelPortali O70628.
    SMRi O70628. Positions 181-504.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei O70628.

    Proteomic databases

    PRIDEi O70628.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000047168 ; ENSMUSP00000038005 ; ENSMUSG00000041119 . [O70628-1 ]
    ENSMUST00000127929 ; ENSMUSP00000117611 ; ENSMUSG00000041119 . [O70628-1 ]
    GeneIDi 18585.
    KEGGi mmu:18585.
    UCSCi uc008buz.2. mouse. [O70628-1 ]

    Organism-specific databases

    CTDi 5152.
    MGIi MGI:1277179. Pde9a.

    Phylogenomic databases

    eggNOGi NOG268427.
    GeneTreei ENSGT00730000110321.
    HOGENOMi HOG000008058.
    HOVERGENi HBG053545.
    InParanoidi O70628.
    KOi K13761.
    OMAi PRAFKIN.
    OrthoDBi EOG7DFXC4.
    PhylomeDBi O70628.
    TreeFami TF314638.

    Enzyme and pathway databases

    BRENDAi 3.1.4.35. 3474.

    Miscellaneous databases

    ChiTaRSi PDE9A. mouse.
    NextBioi 294460.
    PROi O70628.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O70628.
    Bgeei O70628.
    CleanExi MM_PDE9A.
    Genevestigatori O70628.

    Family and domain databases

    Gene3Di 1.10.1300.10. 1 hit.
    InterProi IPR003607. HD/PDEase_dom.
    IPR023088. PDEase.
    IPR002073. PDEase_catalytic_dom.
    IPR023174. PDEase_CS.
    [Graphical view ]
    Pfami PF00233. PDEase_I. 1 hit.
    [Graphical view ]
    PRINTSi PR00387. PDIESTERASE1.
    SMARTi SM00471. HDc. 1 hit.
    [Graphical view ]
    PROSITEi PS00126. PDEASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification and characterization of a novel family of cyclic nucleotide phosphodiesterases."
      Soderling S.H., Bayuga S.J., Beavo J.A.
      J. Biol. Chem. 273:15553-15558(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Identification and characterization of a novel cyclic nucleotide phosphodiesterase gene (PDE9A) that maps to 21q22.3: alternative splicing of mRNA transcripts, genomic structure and sequence."
      Guipponi M., Scott H.S., Kudoh J., Kawasaki K., Shibuya K., Shintani A., Asakawa S., Chen H., Lalioti M.D., Rossier C., Minoshima S., Shimizu N., Antonarakis S.E.
      Hum. Genet. 103:386-392(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Tissue: Brain.

    Entry informationi

    Entry nameiPDE9A_MOUSE
    AccessioniPrimary (citable) accession number: O70628
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 113 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3