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Protein

High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A

Gene

Pde9a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

a divalent metal cationBy similarityNote: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.By similarity

Enzyme regulationi

Inhibited by SCH 51866 and moderately, by zaprinast.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei251 – 2511Proton donorBy similarity
Metal bindingi255 – 2551Divalent metal cation 1By similarity
Metal bindingi291 – 2911Divalent metal cation 1By similarity
Metal bindingi292 – 2921Divalent metal cation 1By similarity
Metal bindingi292 – 2921Divalent metal cation 2By similarity
Binding sitei292 – 2921cGMPBy similarity
Metal bindingi401 – 4011Divalent metal cation 1By similarity
Binding sitei401 – 4011cGMPBy similarity
Binding sitei423 – 4231cGMPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi251 – 2555cGMPBy similarity
Nucleotide bindingi451 – 4522cGMPBy similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cGMP-mediated signaling Source: Ensembl
  2. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.4.35. 3474.
ReactomeiREACT_311531. cGMP effects.

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A (EC:3.1.4.35)
Gene namesi
Name:Pde9a
Synonyms:Pde8b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1277179. Pde9a.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: MGI
  2. perikaryon Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9APRO_0000198842Add
BLAST

Proteomic databases

MaxQBiO70628.
PRIDEiO70628.

PTM databases

PhosphoSiteiO70628.

Expressioni

Tissue specificityi

Highly expressed in kidney. Lower levels in liver, lung and brain.

Gene expression databases

BgeeiO70628.
CleanExiMM_PDE9A.
ExpressionAtlasiO70628. baseline and differential.
GenevestigatoriO70628.

Structurei

3D structure databases

ProteinModelPortaliO70628.
SMRiO70628. Positions 181-504.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni227 – 489263CatalyticBy similarityAdd
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG268427.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000008058.
HOVERGENiHBG053545.
InParanoidiO70628.
KOiK13761.
OMAiPRAFKIN.
OrthoDBiEOG7DFXC4.
PhylomeDBiO70628.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. AlignAdd to basket

Note: A number of isoforms are produced.

Isoform 1 (identifier: O70628-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAGSSSYRP KAIYLDIDGR IQKVVFSKYC NSSDIMDLFC IATGLPRNTT
60 70 80 90 100
ISLLTTDDAM VSIDPTMPAN SERTPYKVRP VAVKQVSERE ELIQGVLAQV
110 120 130 140 150
AEQFSRAFKI NELKAEVANH LAVLEKRVEL EGLKVVEIEK CKSDIKKMRE
160 170 180 190 200
ELAARNSRTN CPCKYSFLDN KKLTPRRDVP TYPKYLLSPE TIEALRKPTF
210 220 230 240 250
DVWLWEPNEM LSCLEHMYHD LGLVRDFSIN PITLRRWLLC VHDNYRNNPF
260 270 280 290 300
HNFRHCFCVT QMMYSMVWLC GLQEKFSQMD ILVLMTAAIC HDLDHPGYNN
310 320 330 340 350
TYQINARTEL AVRYNDISPL ENHHCAIAFQ ILARPECNIF ASVPPEGFRQ
360 370 380 390 400
IRQGMITLIL ATDMARHAEI MDSFKEKMEN FDYSNEEHLT LLKMILIKCC
410 420 430 440 450
DISNEVRPME VAEPWVDCLL EEYFMQSDRE KSEGLPVAPF MDRDKVTKAT
460 470 480 490 500
AQIGFIKFVL IPMFETVTKL FPVVEETMLR PLWESREHYE ELKQLDDAMK
510 520 530
ELQKKTESLT SGAPENTTEK NRDAKDSEGH SPPN
Length:534
Mass (Da):61,636
Last modified:July 31, 1998 - v1
Checksum:i28126C7BB7375241
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031147 mRNA. Translation: AAC24344.1.
AF068247 mRNA. Translation: AAC23996.1.
BC061163 mRNA. Translation: AAH61163.1.
CCDSiCCDS28605.1. [O70628-1]
RefSeqiNP_001157220.1. NM_001163748.1. [O70628-1]
NP_032830.3. NM_008804.4. [O70628-1]
UniGeneiMm.10812.

Genome annotation databases

EnsembliENSMUST00000047168; ENSMUSP00000038005; ENSMUSG00000041119. [O70628-1]
ENSMUST00000127929; ENSMUSP00000117611; ENSMUSG00000041119. [O70628-1]
GeneIDi18585.
KEGGimmu:18585.
UCSCiuc008buz.2. mouse. [O70628-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF031147 mRNA. Translation: AAC24344.1.
AF068247 mRNA. Translation: AAC23996.1.
BC061163 mRNA. Translation: AAH61163.1.
CCDSiCCDS28605.1. [O70628-1]
RefSeqiNP_001157220.1. NM_001163748.1. [O70628-1]
NP_032830.3. NM_008804.4. [O70628-1]
UniGeneiMm.10812.

3D structure databases

ProteinModelPortaliO70628.
SMRiO70628. Positions 181-504.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiO70628.

Proteomic databases

MaxQBiO70628.
PRIDEiO70628.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000047168; ENSMUSP00000038005; ENSMUSG00000041119. [O70628-1]
ENSMUST00000127929; ENSMUSP00000117611; ENSMUSG00000041119. [O70628-1]
GeneIDi18585.
KEGGimmu:18585.
UCSCiuc008buz.2. mouse. [O70628-1]

Organism-specific databases

CTDi5152.
MGIiMGI:1277179. Pde9a.

Phylogenomic databases

eggNOGiNOG268427.
GeneTreeiENSGT00760000118889.
HOGENOMiHOG000008058.
HOVERGENiHBG053545.
InParanoidiO70628.
KOiK13761.
OMAiPRAFKIN.
OrthoDBiEOG7DFXC4.
PhylomeDBiO70628.
TreeFamiTF314638.

Enzyme and pathway databases

BRENDAi3.1.4.35. 3474.
ReactomeiREACT_311531. cGMP effects.

Miscellaneous databases

NextBioi294460.
PROiO70628.
SOURCEiSearch...

Gene expression databases

BgeeiO70628.
CleanExiMM_PDE9A.
ExpressionAtlasiO70628. baseline and differential.
GenevestigatoriO70628.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel family of cyclic nucleotide phosphodiesterases."
    Soderling S.H., Bayuga S.J., Beavo J.A.
    J. Biol. Chem. 273:15553-15558(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification and characterization of a novel cyclic nucleotide phosphodiesterase gene (PDE9A) that maps to 21q22.3: alternative splicing of mRNA transcripts, genomic structure and sequence."
    Guipponi M., Scott H.S., Kudoh J., Kawasaki K., Shibuya K., Shintani A., Asakawa S., Chen H., Lalioti M.D., Rossier C., Minoshima S., Shimizu N., Antonarakis S.E.
    Hum. Genet. 103:386-392(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.

Entry informationi

Entry nameiPDE9A_MOUSE
AccessioniPrimary (citable) accession number: O70628
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 14, 1999
Last sequence update: July 31, 1998
Last modified: March 31, 2015
This is version 118 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.