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O70628

- PDE9A_MOUSE

UniProt

O70628 - PDE9A_MOUSE

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Protein
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
Gene
Pde9a, Pde8b
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.

Catalytic activityi

Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.

Cofactori

Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.

Enzyme regulationi

Inhibited by SCH 51866 and moderately, by zaprinast.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei251 – 2511Proton donor By similarity
Metal bindingi255 – 2551Divalent metal cation 1 By similarity
Metal bindingi291 – 2911Divalent metal cation 1 By similarity
Metal bindingi292 – 2921Divalent metal cation 1 By similarity
Metal bindingi292 – 2921Divalent metal cation 2 By similarity
Binding sitei292 – 2921cGMP By similarity
Metal bindingi401 – 4011Divalent metal cation 1 By similarity
Binding sitei401 – 4011cGMP By similarity
Binding sitei423 – 4231cGMP By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi251 – 2555cGMP By similarity
Nucleotide bindingi451 – 4522cGMP By similarity

GO - Molecular functioni

  1. 3',5'-cyclic-GMP phosphodiesterase activity Source: MGI
  2. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. cGMP-mediated signaling Source: Ensembl
  2. metabolic process Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

cGMP, Manganese, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.4.35. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A (EC:3.1.4.35)
Gene namesi
Name:Pde9a
Synonyms:Pde8b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:1277179. Pde9a.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. perikaryon Source: Ensembl
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 534534High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
PRO_0000198842Add
BLAST

Proteomic databases

PRIDEiO70628.

PTM databases

PhosphoSiteiO70628.

Expressioni

Tissue specificityi

Highly expressed in kidney. Lower levels in liver, lung and brain.

Gene expression databases

ArrayExpressiO70628.
BgeeiO70628.
CleanExiMM_PDE9A.
GenevestigatoriO70628.

Structurei

3D structure databases

ProteinModelPortaliO70628.
SMRiO70628. Positions 181-504.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni227 – 489263Catalytic By similarity
Add
BLAST

Domaini

Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG268427.
GeneTreeiENSGT00730000110321.
HOGENOMiHOG000008058.
HOVERGENiHBG053545.
InParanoidiO70628.
KOiK13761.
OMAiPRAFKIN.
OrthoDBiEOG7DFXC4.
PhylomeDBiO70628.
TreeFamiTF314638.

Family and domain databases

Gene3Di1.10.1300.10. 1 hit.
InterProiIPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view]
PfamiPF00233. PDEase_I. 1 hit.
[Graphical view]
PRINTSiPR00387. PDIESTERASE1.
SMARTiSM00471. HDc. 1 hit.
[Graphical view]
PROSITEiPS00126. PDEASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

This entry describes 1 isoform i produced by alternative splicing. Align

Note: A number of isoforms are produced.

Isoform 1 (identifier: O70628-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

MGAGSSSYRP KAIYLDIDGR IQKVVFSKYC NSSDIMDLFC IATGLPRNTT    50
ISLLTTDDAM VSIDPTMPAN SERTPYKVRP VAVKQVSERE ELIQGVLAQV 100
AEQFSRAFKI NELKAEVANH LAVLEKRVEL EGLKVVEIEK CKSDIKKMRE 150
ELAARNSRTN CPCKYSFLDN KKLTPRRDVP TYPKYLLSPE TIEALRKPTF 200
DVWLWEPNEM LSCLEHMYHD LGLVRDFSIN PITLRRWLLC VHDNYRNNPF 250
HNFRHCFCVT QMMYSMVWLC GLQEKFSQMD ILVLMTAAIC HDLDHPGYNN 300
TYQINARTEL AVRYNDISPL ENHHCAIAFQ ILARPECNIF ASVPPEGFRQ 350
IRQGMITLIL ATDMARHAEI MDSFKEKMEN FDYSNEEHLT LLKMILIKCC 400
DISNEVRPME VAEPWVDCLL EEYFMQSDRE KSEGLPVAPF MDRDKVTKAT 450
AQIGFIKFVL IPMFETVTKL FPVVEETMLR PLWESREHYE ELKQLDDAMK 500
ELQKKTESLT SGAPENTTEK NRDAKDSEGH SPPN 534
Length:534
Mass (Da):61,636
Last modified:August 1, 1998 - v1
Checksum:i28126C7BB7375241
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031147 mRNA. Translation: AAC24344.1.
AF068247 mRNA. Translation: AAC23996.1.
BC061163 mRNA. Translation: AAH61163.1.
CCDSiCCDS28605.1. [O70628-1]
RefSeqiNP_001157220.1. NM_001163748.1. [O70628-1]
NP_032830.3. NM_008804.4. [O70628-1]
UniGeneiMm.10812.

Genome annotation databases

EnsembliENSMUST00000047168; ENSMUSP00000038005; ENSMUSG00000041119. [O70628-1]
ENSMUST00000127929; ENSMUSP00000117611; ENSMUSG00000041119. [O70628-1]
GeneIDi18585.
KEGGimmu:18585.
UCSCiuc008buz.2. mouse. [O70628-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF031147 mRNA. Translation: AAC24344.1 .
AF068247 mRNA. Translation: AAC23996.1 .
BC061163 mRNA. Translation: AAH61163.1 .
CCDSi CCDS28605.1. [O70628-1 ]
RefSeqi NP_001157220.1. NM_001163748.1. [O70628-1 ]
NP_032830.3. NM_008804.4. [O70628-1 ]
UniGenei Mm.10812.

3D structure databases

ProteinModelPortali O70628.
SMRi O70628. Positions 181-504.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei O70628.

Proteomic databases

PRIDEi O70628.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000047168 ; ENSMUSP00000038005 ; ENSMUSG00000041119 . [O70628-1 ]
ENSMUST00000127929 ; ENSMUSP00000117611 ; ENSMUSG00000041119 . [O70628-1 ]
GeneIDi 18585.
KEGGi mmu:18585.
UCSCi uc008buz.2. mouse. [O70628-1 ]

Organism-specific databases

CTDi 5152.
MGIi MGI:1277179. Pde9a.

Phylogenomic databases

eggNOGi NOG268427.
GeneTreei ENSGT00730000110321.
HOGENOMi HOG000008058.
HOVERGENi HBG053545.
InParanoidi O70628.
KOi K13761.
OMAi PRAFKIN.
OrthoDBi EOG7DFXC4.
PhylomeDBi O70628.
TreeFami TF314638.

Enzyme and pathway databases

BRENDAi 3.1.4.35. 3474.

Miscellaneous databases

ChiTaRSi PDE9A. mouse.
NextBioi 294460.
PROi O70628.
SOURCEi Search...

Gene expression databases

ArrayExpressi O70628.
Bgeei O70628.
CleanExi MM_PDE9A.
Genevestigatori O70628.

Family and domain databases

Gene3Di 1.10.1300.10. 1 hit.
InterProi IPR003607. HD/PDEase_dom.
IPR023088. PDEase.
IPR002073. PDEase_catalytic_dom.
IPR023174. PDEase_CS.
[Graphical view ]
Pfami PF00233. PDEase_I. 1 hit.
[Graphical view ]
PRINTSi PR00387. PDIESTERASE1.
SMARTi SM00471. HDc. 1 hit.
[Graphical view ]
PROSITEi PS00126. PDEASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification and characterization of a novel family of cyclic nucleotide phosphodiesterases."
    Soderling S.H., Bayuga S.J., Beavo J.A.
    J. Biol. Chem. 273:15553-15558(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Identification and characterization of a novel cyclic nucleotide phosphodiesterase gene (PDE9A) that maps to 21q22.3: alternative splicing of mRNA transcripts, genomic structure and sequence."
    Guipponi M., Scott H.S., Kudoh J., Kawasaki K., Shibuya K., Shintani A., Asakawa S., Chen H., Lalioti M.D., Rossier C., Minoshima S., Shimizu N., Antonarakis S.E.
    Hum. Genet. 103:386-392(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Tissue: Brain.

Entry informationi

Entry nameiPDE9A_MOUSE
AccessioniPrimary (citable) accession number: O70628
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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