O70628 (PDE9A_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
EC=3.1.4.35

Gene names

Name:	Pde9a
Synonyms:	Pde8b

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	534 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
Catalytic activity	Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
Cofactor	Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions By similarity.
Enzyme regulation	Inhibited by SCH 51866 and moderately, by zaprinast.
Tissue specificity	Highly expressed in kidney. Lower levels in liver, lung and brain.
Domain	Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
Sequence similarities	Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	Manganese Metal-binding cGMP
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	cytosol Inferred from electronic annotation. Source: Compara
Molecular_function	3',5'-cyclic-GMP phosphodiesterase activity Inferred from direct assay Ref.1. Source: MGI metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 1 isoform produced by alternative splicing. [Select]

Note: A number of isoforms are produced.

Isoform 1 (identifier: O70628-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 534	534	High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A		PRO_0000198842
Regions
	Nucleotide binding	251 – 255	5	cGMP By similarity
	Nucleotide binding	451 – 452	2	cGMP By similarity
	Region	227 – 489	263	Catalytic By similarity
Sites
	Active site	251	1	Proton donor By similarity
	Metal binding	255	1	Divalent metal cation 1 By similarity
	Metal binding	291	1	Divalent metal cation 1 By similarity
	Metal binding	292	1	Divalent metal cation 1 By similarity
	Metal binding	292	1	Divalent metal cation 2 By similarity
	Metal binding	401	1	Divalent metal cation 1 By similarity
	Binding site	292	1	cGMP By similarity
	Binding site	401	1	cGMP By similarity
	Binding site	423	1	cGMP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 28126C7BB7375241	FASTA	534	61,636
	10 20 30 40 50 60 MGAGSSSYRP KAIYLDIDGR IQKVVFSKYC NSSDIMDLFC IATGLPRNTT ISLLTTDDAM 70 80 90 100 110 120 VSIDPTMPAN SERTPYKVRP VAVKQVSERE ELIQGVLAQV AEQFSRAFKI NELKAEVANH 130 140 150 160 170 180 LAVLEKRVEL EGLKVVEIEK CKSDIKKMRE ELAARNSRTN CPCKYSFLDN KKLTPRRDVP 190 200 210 220 230 240 TYPKYLLSPE TIEALRKPTF DVWLWEPNEM LSCLEHMYHD LGLVRDFSIN PITLRRWLLC 250 260 270 280 290 300 VHDNYRNNPF HNFRHCFCVT QMMYSMVWLC GLQEKFSQMD ILVLMTAAIC HDLDHPGYNN 310 320 330 340 350 360 TYQINARTEL AVRYNDISPL ENHHCAIAFQ ILARPECNIF ASVPPEGFRQ IRQGMITLIL 370 380 390 400 410 420 ATDMARHAEI MDSFKEKMEN FDYSNEEHLT LLKMILIKCC DISNEVRPME VAEPWVDCLL 430 440 450 460 470 480 EEYFMQSDRE KSEGLPVAPF MDRDKVTKAT AQIGFIKFVL IPMFETVTKL FPVVEETMLR 490 500 510 520 530 PLWESREHYE ELKQLDDAMK ELQKKTESLT SGAPENTTEK NRDAKDSEGH SPPN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification and characterization of a novel family of cyclic nucleotide phosphodiesterases." Soderling S.H., Bayuga S.J., Beavo J.A. J. Biol. Chem. 273:15553-15558(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Identification and characterization of a novel cyclic nucleotide phosphodiesterase gene (PDE9A) that maps to 21q22.3: alternative splicing of mRNA transcripts, genomic structure and sequence." Guipponi M., Scott H.S., Kudoh J., Kawasaki K., Shibuya K., Shintani A., Asakawa S., Chen H., Lalioti M.D., Rossier C., Minoshima S., Shimizu N., Antonarakis S.E. Hum. Genet. 103:386-392(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF031147 mRNA. Translation: AAC24344.1. AF068247 mRNA. Translation: AAC23996.1. BC061163 mRNA. Translation: AAH61163.1.
IPI	IPI00120438.
RefSeq	NP_001157220.1. NM_001163748.1. NP_032830.3. NM_008804.4.
UniGene	Mm.10812.
3D structure databases
ProteinModelPortal	O70628.
SMR	O70628. Positions 181-504.
ModBase	Search...
PTM databases
PhosphoSite	O70628.
Proteomic databases
PRIDE	O70628.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000047168; ENSMUSP00000038005; ENSMUSG00000041119. ENSMUST00000127929; ENSMUSP00000117611; ENSMUSG00000041119.
GeneID	18585.
KEGG	mmu:18585.
UCSC	uc008buz.2. mouse.
Organism-specific databases
CTD	5152.
MGI	MGI:1277179. Pde9a.
Phylogenomic databases
eggNOG	NOG268427.
GeneTree	ENSGT00690000101749.
HOGENOM	HOG000008058.
HOVERGEN	HBG053545.
InParanoid	O70628.
KO	K13761.
OMA	YPKYMLS.
OrthoDB	EOG4G4GQ5.
Enzyme and pathway databases
BRENDA	3.1.4.35. 3474.
Gene expression databases
ArrayExpress	O70628.
Bgee	O70628.
CleanEx	MM_PDE9A.
Genevestigator	O70628.
GermOnline	ENSMUSG00000041119. Mus musculus.
Family and domain databases
Gene3D	1.10.1300.10. 1 hit.
InterPro	IPR003607. HD/PDEase_dom. IPR023088. PDEase. IPR002073. PDEase_catalytic_dom. IPR023174. PDEase_CS. [Graphical view]
Pfam	PF00233. PDEase_I. 1 hit. [Graphical view]
PRINTS	PR00387. PDIESTERASE1.
SMART	SM00471. HDc. 1 hit. [Graphical view]
PROSITE	PS00126. PDEASE_I. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	PDE9A. mouse.
NextBio	294460.
SOURCE	Search...

Entry information

Entry name

PDE9A_MOUSE

Accession

Primary (citable) accession number: O70628

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 15, 1999
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program