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O70617 (IRK13_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Inward rectifier potassium channel 13
Alternative name(s):
Inward rectifier K(+) channel Kir7.1
Potassium channel, inwardly rectifying subfamily J member 13
Gene names
Name:Kcnj13
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length360 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ13 has a very low single channel conductance, low sensitivity to block by external barium and cesium, and no dependence of its inward rectification properties on the internal blocking particle magnesium.

Subcellular location

Membrane; Multi-pass membrane protein.

Post-translational modification

Phosphorylation at Ser-201 by PKC strongly inhibits ionic currents, while phosphorylation at Ser-287 by PKA increases them By similarity.

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ13 subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Potassium transport
Transport
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   LigandPotassium
   Molecular functionIon channel
Voltage-gated channel
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functioninward rectifier potassium channel activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 360360Inward rectifier potassium channel 13
PRO_0000154967

Regions

Topological domain1 – 5353Cytoplasmic By similarity
Transmembrane54 – 7825Helical; Name=M1; By similarity
Topological domain79 – 10527Extracellular By similarity
Intramembrane106 – 11712Helical; Pore-forming; Name=H5; By similarity
Intramembrane118 – 1247Pore-forming; By similarity
Topological domain125 – 1339Extracellular By similarity
Transmembrane134 – 15522Helical; Name=M2; By similarity
Topological domain156 – 360205Cytoplasmic By similarity
Motif119 – 1246Selectivity filter By similarity

Sites

Site1491Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity

Amino acid modifications

Modified residue2011Phosphoserine; by PKC By similarity
Modified residue2871Phosphoserine; by PKA By similarity

Sequences

Sequence LengthMass (Da)Tools
O70617 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: B3D2F6CB8F99FD16

FASTA36040,637
        10         20         30         40         50         60 
MDSRNCKVNA PLLSQRYRRM VTKDGHSTLQ MDGAQRGLVY LRDAWGILMD MRWRWMMLVF 

        70         80         90        100        110        120 
SASFVVHWLV FAVLWYAVAE MNGDLEIDHD VPPENHTICV KHITSFTAAF SFSLETQLTI 

       130        140        150        160        170        180 
GYGTMFPSGD CPSAIALLAI QMLLGLMLEA FITGAFVAKI ARPKNRAFSI RFTDLAVVAH 

       190        200        210        220        230        240 
KDGKPNLIFQ VANTRPSPLT SVRVSAVLYQ ERENGELYQT SVDFHLDGIS SEECPFFIFP 

       250        260        270        280        290        300 
LTYYHTITPS SPLATLLQHE TPSHFELVVF LSAMQEGTGE ICQRRTSYLP SEIMLHHRFA 

       310        320        330        340        350        360 
ALMTRGSKGE YQVKMENFDK TVPEHPTPVV SKSPHRTDLD IHINGQSIDN FQIAETGLTE 

« Hide

References

[1]"Unique epithelial Kir7.1 subunit defines a new subfamily of inwardly rectifying potassium channels."
Doering F., Derst C., Wischmeyer E., Karschin C., Daut J., Karschin A.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Tissue: Brain.
[2]Hirose S., Suzuki Y., Nakamura N.
Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Kidney.
[3]"Complex structure and regulation of expression of the rat gene for inward rectifier potassium channel Kir7.1."
Nakamura N., Suzuki Y., Ikeda Y., Notoya M., Hirose S.
J. Biol. Chem. 275:28276-28284(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Liver.
[4]"Genomic structure and promoter analysis of the rat Kir7.1 potassium channel gene (Kcnj13)."
Doering F., Karschin A.
FEBS Lett. 483:93-98(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Tissue: Liver.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ006129 mRNA. Translation: CAA06879.1.
AB013890 mRNA. Translation: BAA28272.1.
AB034241 Genomic DNA. Translation: BAA97255.1.
AJ292748 Genomic DNA. Translation: CAC17469.1.
RefSeqNP_446060.1. NM_053608.2.
XP_006245544.1. XM_006245482.1.
XP_006245545.1. XM_006245483.1.
XP_006245546.1. XM_006245484.1.
XP_006245547.1. XM_006245485.1.
XP_006245548.1. XM_006245486.1.
XP_006245549.1. XM_006245487.1.
UniGeneRn.14516.

3D structure databases

ProteinModelPortalO70617.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000021507.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000021507; ENSRNOP00000021507; ENSRNOG00000016057.
GeneID94341.
KEGGrno:94341.
UCSCRGD:621661. rat.

Organism-specific databases

CTD3769.
RGD621661. Kcnj13.

Phylogenomic databases

eggNOGNOG280854.
GeneTreeENSGT00750000117737.
HOGENOMHOG000237326.
HOVERGENHBG006178.
InParanoidO70617.
KOK05006.
OMAASFVVHW.
OrthoDBEOG7NW69K.
PhylomeDBO70617.
TreeFamTF313676.

Gene expression databases

GenevestigatorO70617.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR013518. K_chnl_inward-rec_Kir_cyto.
IPR008062. KCNJ13.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PTHR11767:SF3. PTHR11767:SF3. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01679. KIR7CHANNEL.
PR01320. KIRCHANNEL.
SUPFAMSSF81296. SSF81296. 1 hit.
ProtoNetSearch...

Other

NextBio617933.
PROO70617.

Entry information

Entry nameIRK13_RAT
AccessionPrimary (citable) accession number: O70617
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families