O70617 (IRK13_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inward rectifier potassium channel 13

Alternative name(s):
Inward rectifier K(+) channel Kir7.1
Potassium channel, inwardly rectifying subfamily J member 13

Gene names

Name:

Kcnj13

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ13 has a very low single channel conductance, low sensitivity to block by external barium and cesium, and no dependence of its inward rectification properties on the internal blocking particle magnesium.
Subcellular location	Membrane; Multi-pass membrane protein.
Post-translational modification	Phosphorylation at Ser-201 by PKC strongly inhibits ionic currents, while phosphorylation at Ser-287 by PKA increases them By similarity.
Sequence similarities	Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ13 subfamily. [View classification]

Ontologies

Keywords
Biological process	Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
Ligand	Potassium
Molecular function	Ion channel Voltage-gated channel
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	inward rectifier potassium channel activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 360	360	Inward rectifier potassium channel 13		PRO_0000154967
Regions
	Topological domain	1 – 53	53	Cytoplasmic By similarity
	Transmembrane	54 – 78	25	Helical; Name=M1; By similarity
	Topological domain	79 – 105	27	Extracellular By similarity
	Intramembrane	106 – 117	12	Helical; Pore-forming; Name=H5; By similarity
	Intramembrane	118 – 124	7	Pore-forming; By similarity
	Topological domain	125 – 133	9	Extracellular By similarity
	Transmembrane	134 – 155	22	Helical; Name=M2; By similarity
	Topological domain	156 – 360	205	Cytoplasmic By similarity
	Motif	119 – 124	6	Selectivity filter By similarity
Sites
	Site	149	1	Role in the control of polyamine-mediated channel gating and in the blocking by intracellular magnesium By similarity
Amino acid modifications
	Modified residue	201	1	Phosphoserine; by PKC By similarity
	Modified residue	287	1	Phosphoserine; by PKA By similarity
	Modified residue	327	1	Phosphothreonine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O70617 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: B3D2F6CB8F99FD16	FASTA	360	40,637
	10 20 30 40 50 60 MDSRNCKVNA PLLSQRYRRM VTKDGHSTLQ MDGAQRGLVY LRDAWGILMD MRWRWMMLVF 70 80 90 100 110 120 SASFVVHWLV FAVLWYAVAE MNGDLEIDHD VPPENHTICV KHITSFTAAF SFSLETQLTI 130 140 150 160 170 180 GYGTMFPSGD CPSAIALLAI QMLLGLMLEA FITGAFVAKI ARPKNRAFSI RFTDLAVVAH 190 200 210 220 230 240 KDGKPNLIFQ VANTRPSPLT SVRVSAVLYQ ERENGELYQT SVDFHLDGIS SEECPFFIFP 250 260 270 280 290 300 LTYYHTITPS SPLATLLQHE TPSHFELVVF LSAMQEGTGE ICQRRTSYLP SEIMLHHRFA 310 320 330 340 350 360 ALMTRGSKGE YQVKMENFDK TVPEHPTPVV SKSPHRTDLD IHINGQSIDN FQIAETGLTE

References

[1]	"Unique epithelial Kir7.1 subunit defines a new subfamily of inwardly rectifying potassium channels." Doering F., Derst C., Wischmeyer E., Karschin C., Daut J., Karschin A. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Tissue: Brain.
[2]	Hirose S., Suzuki Y., Nakamura N. Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Kidney.
[3]	"Complex structure and regulation of expression of the rat gene for inward rectifier potassium channel Kir7.1." Nakamura N., Suzuki Y., Ikeda Y., Notoya M., Hirose S. J. Biol. Chem. 275:28276-28284(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
[4]	"Genomic structure and promoter analysis of the rat Kir7.1 potassium channel gene (Kcnj13)." Doering F., Karschin A. FEBS Lett. 483:93-98(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ006129 mRNA. Translation: CAA06879.1. AB013890 mRNA. Translation: BAA28272.1. AB034241 Genomic DNA. Translation: BAA97255.1. AJ292748 Genomic DNA. Translation: CAC17469.1.
IPI	IPI00200406.
RefSeq	NP_446060.1. NM_053608.2.
UniGene	Rn.14516.
3D structure databases
ProteinModelPortal	O70617.
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000021507.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000021507; ENSRNOP00000021507; ENSRNOG00000016057.
GeneID	94341.
KEGG	rno:94341.
UCSC	RGD:621661. rat.
Organism-specific databases
CTD	3769.
RGD	621661. Kcnj13.
Phylogenomic databases
eggNOG	NOG280854.
GeneTree	ENSGT00690000102210.
HOGENOM	HOG000237326.
HOVERGEN	HBG006178.
InParanoid	O70617.
KO	K05006.
OMA	YYHSITP.
OrthoDB	EOG44J2JS.
Gene expression databases
Genevestigator	O70617.
Family and domain databases
Gene3D	2.60.40.1400. 1 hit.
InterPro	IPR014756. Ig_E-set. IPR016449. K_chnl_inward-rec_Kir. IPR008062. K_chnl_inward-rec_Kir7. IPR013518. K_chnl_inward-rec_Kir_cyto. [Graphical view]
PANTHER	PTHR11767. PTHR11767. 1 hit. PTHR11767:SF3. PTHR11767:SF3. 1 hit.
Pfam	PF01007. IRK. 1 hit. [Graphical view]
PIRSF	PIRSF005465. GIRK_kir. 1 hit.
PRINTS	PR01679. KIR7CHANNEL. PR01320. KIRCHANNEL.
SUPFAM	SSF81296. Ig_E-set. 1 hit.
ProtoNet	Search...
Other
NextBio	617933.

Entry information

Entry name

IRK13_RAT

Accession

Primary (citable) accession number: O70617

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 27, 2001
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program