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Protein

Linker for activation of T-cells family member 1

Gene

Lat

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules (By similarity).By similarity

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Adaptive immunity, Immunity, Mast cell degranulation

Enzyme and pathway databases

ReactomeiR-RNO-114604. GPVI-mediated activation cascade.
R-RNO-202433. Generation of second messenger molecules.
R-RNO-2424491. DAP12 signaling.
R-RNO-2871796. FCERI mediated MAPK activation.
R-RNO-2871809. FCERI mediated Ca+2 mobilization.
R-RNO-5673001. RAF/MAP kinase cascade.

Names & Taxonomyi

Protein namesi
Recommended name:
Linker for activation of T-cells family member 1
Alternative name(s):
36 kDa phospho-tyrosine adapter protein
Short name:
pp36
p36-38
Gene namesi
Name:Lat
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620802. Lat.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 4ExtracellularSequence analysis4
Transmembranei5 – 28Helical; Signal-anchor for type III membrane proteinSequence analysisAdd BLAST24
Topological domaini29 – 241CytoplasmicSequence analysisAdd BLAST213

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000833271 – 241Linker for activation of T-cells family member 1Add BLAST241

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi27S-palmitoyl cysteineBy similarity1
Lipidationi30S-palmitoyl cysteineBy similarity1
Modified residuei41PhosphoserineCombined sources1
Modified residuei44PhosphoserineBy similarity1
Modified residuei87PhosphoserineBy similarity1
Modified residuei104PhosphoserineCombined sources1
Modified residuei109PhosphoserineCombined sources1
Modified residuei112PhosphoserineCombined sources1
Modified residuei175PhosphotyrosineBy similarity1
Modified residuei199PhosphoserineCombined sources1
Modified residuei212PhosphoserineBy similarity1
Modified residuei215PhosphoserineBy similarity1
Modified residuei234PhosphotyrosineBy similarity1

Post-translational modificationi

Phosphorylated on tyrosines by ZAP70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement. May be dephosphorylated by PTPRJ (By similarity).By similarity
Palmitoylation of Cys-27 and Cys-30 is required for raft targeting and efficient phosphorylation.By similarity

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiO70601.

PTM databases

iPTMnetiO70601.
PhosphoSitePlusiO70601.

Expressioni

Tissue specificityi

Expressed in NK cells. Present in lymph node, spleen and thymus (at protein level).1 Publication

Gene expression databases

BgeeiENSRNOG00000017429.
GenevisibleiO70601. RN.

Interactioni

Subunit structurei

When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK. Interacts with FCGR1A. Interacts with GRB2, PLCG1 and THEMIS upon TCR activation in thymocytes (By similarity).By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023563.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 139Interaction with PLCG1By similarity4
Regioni175 – 178Interaction with GRB2, GRAP2 and PIK3R1By similarity4
Regioni195 – 198Interaction with GRB2, GRAP2 and PIK3R1By similarity4

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IW3X. Eukaryota.
ENOG4111EFT. LUCA.
GeneTreeiENSGT00390000014223.
HOGENOMiHOG000081810.
HOVERGENiHBG018198.
InParanoidiO70601.
KOiK07362.
OMAiGSHRMPS.
OrthoDBiEOG091G0QKO.
PhylomeDBiO70601.
TreeFamiTF337741.

Family and domain databases

InterProiIPR008359. Linker_for_activat_Tcells_prot.
[Graphical view]
PANTHERiPTHR15586. PTHR15586. 1 hit.
PfamiPF15234. LAT. 1 hit.
[Graphical view]
PRINTSiPR01781. LATPROTEIN.

Sequencei

Sequence statusi: Complete.

O70601-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEADALSPVE LGLLLLPFVV MLLAALCVRC RELPASYDSA STESLYPRSI
60 70 80 90 100
LIKPPQITVP RTPATSYPLV TSFPPLRQPD LLPIPRSPQP LGGSHRMPSS
110 120 130 140 150
RQNSDDANSV ASYENQEPAR KNVDEDEDED DYPEGYLVVL PDSSPAAVPV
160 170 180 190 200
VSSAPVPSNP DLGDSAFSME SCEDYVNVPE SEESAEASLD GSREYVNVSQ
210 220 230 240
DAQPVIRAEL ASVTSQEVED EEEEDVDGEE APDYENLQEL N
Length:241
Mass (Da):26,193
Last modified:August 1, 1998 - v1
Checksum:iFBD5C4D4BBCE4275
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001184 mRNA. Translation: CAA04577.1.
RefSeqiNP_110480.1. NM_030853.1.
UniGeneiRn.9773.

Genome annotation databases

EnsembliENSRNOT00000023563; ENSRNOP00000023563; ENSRNOG00000017429.
GeneIDi81511.
KEGGirno:81511.
UCSCiRGD:620802. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001184 mRNA. Translation: CAA04577.1.
RefSeqiNP_110480.1. NM_030853.1.
UniGeneiRn.9773.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000023563.

PTM databases

iPTMnetiO70601.
PhosphoSitePlusiO70601.

Proteomic databases

PaxDbiO70601.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000023563; ENSRNOP00000023563; ENSRNOG00000017429.
GeneIDi81511.
KEGGirno:81511.
UCSCiRGD:620802. rat.

Organism-specific databases

CTDi27040.
RGDi620802. Lat.

Phylogenomic databases

eggNOGiENOG410IW3X. Eukaryota.
ENOG4111EFT. LUCA.
GeneTreeiENSGT00390000014223.
HOGENOMiHOG000081810.
HOVERGENiHBG018198.
InParanoidiO70601.
KOiK07362.
OMAiGSHRMPS.
OrthoDBiEOG091G0QKO.
PhylomeDBiO70601.
TreeFamiTF337741.

Enzyme and pathway databases

ReactomeiR-RNO-114604. GPVI-mediated activation cascade.
R-RNO-202433. Generation of second messenger molecules.
R-RNO-2424491. DAP12 signaling.
R-RNO-2871796. FCERI mediated MAPK activation.
R-RNO-2871809. FCERI mediated Ca+2 mobilization.
R-RNO-5673001. RAF/MAP kinase cascade.

Miscellaneous databases

PROiO70601.

Gene expression databases

BgeeiENSRNOG00000017429.
GenevisibleiO70601. RN.

Family and domain databases

InterProiIPR008359. Linker_for_activat_Tcells_prot.
[Graphical view]
PANTHERiPTHR15586. PTHR15586. 1 hit.
PfamiPF15234. LAT. 1 hit.
[Graphical view]
PRINTSiPR01781. LATPROTEIN.
ProtoNetiSearch...

Entry informationi

Entry nameiLAT_RAT
AccessioniPrimary (citable) accession number: O70601
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell-induced activation of PLC, maybe by inducing the dephosphorylation of LAT.By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.