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Reviewed, UniProtKB/Swiss-Prot O70601 (LAT_RAT)

Last modified January 19, 2010. Version 63. Feed History...

Clusters with 100%, 90%, 50% identity | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information

Names and origin

Protein namesRecommended name:
    Linker for activation of T-cells family member 1
Alternative name(s):
    36 kDa phospho-tyrosine adapter protein
    pp36
    p36-38
Gene names
Name: Lat
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules By similarity.

Subunit structure

When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK. Interacts with FCGR1A.

Subcellular location

Cell membrane; Single-pass type III membrane protein By similarity. Note: Present in lipid rafts By similarity.

Tissue specificity

Expressed in NK cells. Present in lymph node, spleen and thymus (at protein level). Ref.2

Post-translational modification

Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement By similarity.

Palmitoylation of Cys-27 and Cys-30 is required for raft targeting and efficient phosphorylation By similarity.

Miscellaneous

Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell-induced activation of PLC, maybe by inducing the dephosphorylation of LAT By similarity.

Ontologies

Keywords
   Biological processImmune response
Mast cell degranulation
   Cellular componentCell membrane
Membrane
   DomainSignal-anchor
Transmembrane
   PTMLipoprotein
Palmitate
Phosphoprotein
Gene Ontology (GO)
   Biological processmast cell degranulation

Inferred from electronic annotation. Source: UniProtKB-KW

signal transduction

Inferred from electronic annotation. Source: InterPro

   Cellular componentintegral to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 241241Linker for activation of T-cells family member 1
PRO_0000083327

Regions

Topological domain1 – 44Extracellular Potential
Transmembrane5 – 2824Signal-anchor for type III membrane protein Potential
Topological domain29 – 241213Cytoplasmic Potential
Region136 – 1394Interaction with PLCG1 By similarity
Region175 – 1784Interaction with GRB2, GRAP2 and PIK3R1 By similarity
Region195 – 1984Interaction with GRB2, GRAP2 and PIK3R1 By similarity

Amino acid modifications

Modified residue361Phosphoserine By similarity
Modified residue411Phosphoserine By similarity
Modified residue441Phosphoserine By similarity
Modified residue871Phosphoserine By similarity
Modified residue1041Phosphoserine By similarity
Modified residue1091Phosphoserine By similarity
Modified residue1121Phosphoserine By similarity
Modified residue1131Phosphotyrosine By similarity
Modified residue1321Phosphotyrosine By similarity
Modified residue1361Phosphotyrosine By similarity
Modified residue1751Phosphotyrosine By similarity
Modified residue1951Phosphotyrosine By similarity
Modified residue1991Phosphoserine By similarity
Modified residue2151Phosphoserine By similarity
Modified residue2341Phosphotyrosine By similarity
Lipidation271S-palmitoyl cysteine By similarity
Lipidation301S-palmitoyl cysteine By similarity

Sequences

Sequence LengthMass (Da)Tools
O70601-1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: FBD5C4D4BBCE4275

FASTA24126,193
        10         20         30         40         50         60 
MEADALSPVE LGLLLLPFVV MLLAALCVRC RELPASYDSA STESLYPRSI LIKPPQITVP 

        70         80         90        100        110        120 
RTPATSYPLV TSFPPLRQPD LLPIPRSPQP LGGSHRMPSS RQNSDDANSV ASYENQEPAR 

       130        140        150        160        170        180 
KNVDEDEDED DYPEGYLVVL PDSSPAAVPV VSSAPVPSNP DLGDSAFSME SCEDYVNVPE 

       190        200        210        220        230        240 
SEESAEASLD GSREYVNVSQ DAQPVIRAEL ASVTSQEVED EEEEDVDGEE APDYENLQEL 


N 

« Hide

References

[1]"Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells."
Weber J.R., Orstavik S., Torgersen K.M., Danbolt N.C., Berg S.F., Ryan J.C., Tasken K., Imboden J.B., Vaage J.T.
J. Exp. Med. 187:1157-1161(1998) [PubMed: 9529333] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: PVG.
Tissue: Natural killer cell.
[2]"Transmembrane adaptor molecules: a new category of lymphoid-cell markers."
Tedoldi S., Paterson J.C., Hansmann M.-L., Natkunam Y., Rudiger T., Angelisova P., Du M.Q., Roberton H., Roncador G., Sanchez L., Pozzobon M., Masir N., Barry R., Pileri S., Mason D.Y., Marafioti T., Horejsi V.
Blood 107:213-221(2006) [PubMed: 16160011] [Abstract]
Cited for: TISSUE SPECIFICITY.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ001184 mRNA. Translation: CAA04577.1.
IPIIPI00200381.
RefSeqNP_110480.1.
UniGeneRn.9773

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

STRINGO70601.

Genome annotation databases

EnsemblENSRNOT00000023563; ENSRNOP00000023563; ENSRNOG00000017429; Rattus norvegicus. [Genome view]
GeneID81511.
KEGGrno:81511.
UCSCBC088130. rat.

Organism-specific databases

CTD81511.
RGD620802. Lat.

Phylogenomic databases

eggNOGmaNOG21725.
HOVERGENO70601.
InParanoidO70601.
OMAEAPDYEN.
OrthoDBEOG9PK4VG.
PhylomeDBO70601.

Gene expression databases

ArrayExpressO70601.
GenevestigatorO70601.
GermOnlineENSRNOG00000017429. Rattus norvegicus.

Family and domain databases

InterProIPR008359. Linker_for_activat_Tcells_prot.
[Graphical view]
PRINTSPR01781. LATPROTEIN.
ProtoNetSearch...

Other Resources

NextBio614989.

Entry information

Entry nameLAT_RAT
AccessionPrimary (citable) accession number: O70601
Entry history
Integrated into UniProtKB/Swiss-Prot: June 1, 2001
Last sequence update: August 1, 1998
Last modified: January 19, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information