Reviewed, UniProtKB/Swiss-Prot O70601 (LAT_RAT)

Names and origin

Protein names

Recommended name:
    Linker for activation of T-cells family member 1
Alternative name(s):
    36 kDa phospho-tyrosine adapter protein
    pp36
    p36-38

Gene names

Name:

Lat

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for TCR (T-cell antigen receptor)- and pre-TCR-mediated signaling, both in mature T-cells and during their development. Involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Couples activation of these receptors and their associated kinases with distal intracellular events such as mobilization of intracellular calcium stores, PKC activation, MAPK activation or cytoskeletal reorganization through the recruitment of PLCG1, GRB2, GRAP2, and other signaling molecules By similarity.
Subunit structure	When phosphorylated, interacts directly with the PIK3R1 subunit of phosphoinositide 3-kinase and the SH2 domains of GRB2, GRAP, GRAP2, PLCG1 and PLCG2. Interacts indirectly with CBL, SOS, VAV, and LCP2. Interacts with SHB, SKAP2 and CLNK. Interacts with FCGR1A.
Subcellular location	Cell membrane; Single-pass type III membrane protein By similarity. Note: Present in lipid rafts By similarity.
Tissue specificity	Expressed in NK cells. Present in lymph node, spleen and thymus (at protein level). Ref.2
Post-translational modification	Phosphorylated on tyrosines by ZAP-70 upon TCR activation, or by SYK upon other immunoreceptor activation; which leads to the recruitment of multiple signaling molecules. Is one of the most prominently tyrosine-phosphorylated proteins detected following TCR engagement By similarity. Palmitoylation of Cys-27 and Cys-30 is required for raft targeting and efficient phosphorylation By similarity.
Miscellaneous	Engagement of killer inhibitory receptors (KIR) disrupts the interaction of PLCG1 with LAT and blocks target cell-induced activation of PLC, maybe by inducing the dephosphorylation of LAT By similarity.

Ontologies

Keywords
Biological process	Immune response Mast cell degranulation
Cellular component	Cell membrane Membrane
Domain	Signal-anchor Transmembrane
PTM	Lipoprotein Palmitate Phosphoprotein
Gene Ontology (GO)
Biological process	mast cell degranulation Inferred from electronic annotation. Source: UniProtKB-KW signal transduction Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 241	241	Linker for activation of T-cells family member 1		PRO_0000083327
Regions
	Topological domain	1 – 4	4	Extracellular Potential
	Transmembrane	5 – 28	24	Signal-anchor for type III membrane protein Potential
	Topological domain	29 – 241	213	Cytoplasmic Potential
	Region	136 – 139	4	Interaction with PLCG1 By similarity
	Region	175 – 178	4	Interaction with GRB2, GRAP2 and PIK3R1 By similarity
	Region	195 – 198	4	Interaction with GRB2, GRAP2 and PIK3R1 By similarity
Amino acid modifications
	Modified residue	36	1	Phosphoserine By similarity
	Modified residue	41	1	Phosphoserine By similarity
	Modified residue	44	1	Phosphoserine By similarity
	Modified residue	87	1	Phosphoserine By similarity
	Modified residue	104	1	Phosphoserine By similarity
	Modified residue	109	1	Phosphoserine By similarity
	Modified residue	112	1	Phosphoserine By similarity
	Modified residue	113	1	Phosphotyrosine By similarity
	Modified residue	132	1	Phosphotyrosine By similarity
	Modified residue	136	1	Phosphotyrosine By similarity
	Modified residue	175	1	Phosphotyrosine By similarity
	Modified residue	195	1	Phosphotyrosine By similarity
	Modified residue	199	1	Phosphoserine By similarity
	Modified residue	215	1	Phosphoserine By similarity
	Modified residue	234	1	Phosphotyrosine By similarity
	Lipidation	27	1	S-palmitoyl cysteine By similarity
	Lipidation	30	1	S-palmitoyl cysteine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O70601-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: FBD5C4D4BBCE4275	FASTA	241	26,193
	10 20 30 40 50 60 MEADALSPVE LGLLLLPFVV MLLAALCVRC RELPASYDSA STESLYPRSI LIKPPQITVP 70 80 90 100 110 120 RTPATSYPLV TSFPPLRQPD LLPIPRSPQP LGGSHRMPSS RQNSDDANSV ASYENQEPAR 130 140 150 160 170 180 KNVDEDEDED DYPEGYLVVL PDSSPAAVPV VSSAPVPSNP DLGDSAFSME SCEDYVNVPE 190 200 210 220 230 240 SEESAEASLD GSREYVNVSQ DAQPVIRAEL ASVTSQEVED EEEEDVDGEE APDYENLQEL N

References

[1]	"Molecular cloning of the cDNA encoding pp36, a tyrosine-phosphorylated adaptor protein selectively expressed by T cells and natural killer cells." Weber J.R., Orstavik S., Torgersen K.M., Danbolt N.C., Berg S.F., Ryan J.C., Tasken K., Imboden J.B., Vaage J.T. J. Exp. Med. 187:1157-1161(1998) [PubMed: 9529333] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: PVG. Tissue: Natural killer cell.
[2]	"Transmembrane adaptor molecules: a new category of lymphoid-cell markers." Tedoldi S., Paterson J.C., Hansmann M.-L., Natkunam Y., Rudiger T., Angelisova P., Du M.Q., Roberton H., Roncador G., Sanchez L., Pozzobon M., Masir N., Barry R., Pileri S., Mason D.Y., Marafioti T., Horejsi V. Blood 107:213-221(2006) [PubMed: 16160011] [Abstract] Cited for: TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AJ001184 mRNA. Translation: CAA04577.1.
IPI	IPI00200381.
RefSeq	NP_110480.1.
UniGene	Rn.9773
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O70601.
Genome annotation databases
Ensembl	ENSRNOT00000023563; ENSRNOP00000023563; ENSRNOG00000017429; Rattus norvegicus. [Genome view]
GeneID	81511.
KEGG	rno:81511.
UCSC	BC088130. rat.
Organism-specific databases
CTD	81511.
RGD	620802. Lat.
Phylogenomic databases
eggNOG	maNOG21725.
HOVERGEN	O70601.
InParanoid	O70601.
OMA	EAPDYEN.
OrthoDB	EOG9PK4VG.
PhylomeDB	O70601.
Gene expression databases
ArrayExpress	O70601.
Genevestigator	O70601.
GermOnline	ENSRNOG00000017429. Rattus norvegicus.
Family and domain databases
InterPro	IPR008359. Linker_for_activat_Tcells_prot. [Graphical view]
PRINTS	PR01781. LATPROTEIN.
ProtoNet	Search...
Other Resources
NextBio	614989.

Entry information

Entry name

LAT_RAT

Accession

Primary (citable) accession number: O70601

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 1, 2001
Last sequence update:	August 1, 1998
Last modified:	January 19, 2010
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)