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O70596 (IRK14_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
ATP-sensitive inward rectifier potassium channel 14
Alternative name(s):
Inward rectifier K(+) channel Kir2.4
Short name=IRK-4
Potassium channel, inwardly rectifying subfamily J member 14
Gene names
Name:Kcnj14
Synonyms:Irk4
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length434 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ14 gives rise to low-conductance channels with a low affinity to the channel blockers Barium and Cesium.

Subcellular location

Membrane; Multi-pass membrane protein.

Tissue specificity

Expressed predominantly in motoneurons of cranial nerve motor nuclei within the general somatic and special visceral motor cell column. Ref.1

Sequence similarities

Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ14 subfamily. [View classification]

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 434434ATP-sensitive inward rectifier potassium channel 14
PRO_0000154970

Regions

Topological domain1 – 8484Cytoplasmic By similarity
Transmembrane85 – 10925Helical; Name=M1; By similarity
Topological domain110 – 13122Extracellular By similarity
Intramembrane132 – 14312Helical; Pore-forming; Name=H5; By similarity
Intramembrane144 – 1507Pore-forming; By similarity
Topological domain151 – 1599Extracellular By similarity
Transmembrane160 – 18122Helical; Name=M2; By similarity
Topological domain182 – 434253Cytoplasmic By similarity
Motif145 – 1506Selectivity filter By similarity

Sequences

Sequence LengthMass (Da)Tools
O70596 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: AB586F4628AB42EA

FASTA43447,609
        10         20         30         40         50         60 
MGLARALRRL SGALEPGNSR AGDEEEAGAG LCRNGWAPGP VAGNRRRGRF VKKDGHCNVR 

        70         80         90        100        110        120 
FVNLGGQGAR YLSDLFTTCV DVRWRWMCLL FSCSFLASWL LFGLTFWLIA SLHGDLAAPP 

       130        140        150        160        170        180 
PPAPCFSQVA SFLAAFLFAL ETQTSIGYGV RSVTEECPAA VAAVVLQCIA GCVLDAFVVG 

       190        200        210        220        230        240 
AVMAKMAKPK KRNETLVFSE NAVVALRDRR LCLMWRVGNL RRSHLVEAHV RAQLLQPRVT 

       250        260        270        280        290        300 
PEGEYIPLDH QDVDVGFDGG TDRIFLVSPI TIVHEIDSAS PLYELGRAEL ARADFELVVI 

       310        320        330        340        350        360 
LEGMVEATAM TTQCRSSYLP GELLWGHRFE PVLFQRGSQY EVDYRHFHRT YEVPGTPVCS 

       370        380        390        400        410        420 
AKELDERAEQ ASHSPKSSFP GSLAAFCYEN ELALSCCQEE DEEEDTKEGT SAETPDRAAS 

       430 
PQALTPTLAL TLPP

« Hide

References

[1]"Kir2.4: a novel K+ inward rectifier channel associated with motoneurons of cranial nerve nuclei."
Toepert C., Doering F., Wischmeyer E., Karschin C., Brockhaus J., Ballanyi K., Derst C., Karschin A.
J. Neurosci. 18:4096-4105(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION, TISSUE SPECIFICITY.
Strain: Sprague-Dawley.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AJ003065 mRNA. Translation: CAA05839.1.
IPIIPI00199587.
RefSeqNP_733836.1. NM_170718.1.
UniGeneRn.154434.

3D structure databases

ProteinModelPortalO70596.
SMRO70596. Positions 190-367.
ModBaseSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000028591.

Protein family/group databases

TCDB1.A.2.1.5. inward rectifier K+ channel (IRK-C) family.

Proteomic databases

PaxDbO70596.
PRIDEO70596.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028591; ENSRNOP00000028591; ENSRNOG00000021056.
GeneID276720.
KEGGrno:276720.

Organism-specific databases

CTD3770.
RGD628872. Kcnj14.

Phylogenomic databases

eggNOGNOG251076.
GeneTreeENSGT00650000093228.
HOVERGENHBG006178.
KOK05007.

Gene expression databases

GenevestigatorO70596.

Family and domain databases

Gene3D2.60.40.1400. 1 hit.
InterProIPR014756. Ig_E-set.
IPR016449. K_chnl_inward-rec_Kir.
IPR013518. K_chnl_inward-rec_Kir_cyto.
[Graphical view]
PANTHERPTHR11767. PTHR11767. 1 hit.
PfamPF01007. IRK. 1 hit.
[Graphical view]
PIRSFPIRSF005465. GIRK_kir. 1 hit.
PRINTSPR01320. KIRCHANNEL.
SUPFAMSSF81296. Ig_E-set. 1 hit.
ProtoNetSearch...

Other

NextBio624761.

Entry information

Entry nameIRK14_RAT
AccessionPrimary (citable) accession number: O70596
Entry history
Integrated into UniProtKB/Swiss-Prot: October 11, 2004
Last sequence update: August 1, 1998
Last modified: April 3, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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