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Protein

Solute carrier family 22 member 5

Gene

Slc22a5

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sodium-ion dependent, high affinity carnitine transporter. Involved in the active cellular uptake of carnitine. Transports one sodium ion with one molecule of carnitine. Also transports organic cations such as tetraethylammonium (TEA) without the involvement of sodium. Also relative uptake activity ratio of carnitine to TEA is 11.3.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi218 – 2258ATPSequence analysis

GO - Molecular functioni

  • antibiotic transporter activity Source: RGD
  • ATP binding Source: UniProtKB-KW
  • carnitine transmembrane transporter activity Source: RGD
  • cation:cation antiporter activity Source: GO_Central
  • quaternary ammonium group transmembrane transporter activity Source: RGD
  • symporter activity Source: UniProtKB-KW

GO - Biological processi

  • antibiotic transport Source: RGD
  • carnitine metabolic process Source: GO_Central
  • carnitine transport Source: RGD
  • quaternary ammonium group transport Source: RGD
  • sodium ion transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Ion transport, Sodium transport, Symport, Transport

Keywords - Ligandi

ATP-binding, Nucleotide-binding, Sodium

Enzyme and pathway databases

ReactomeiR-RNO-549127. Organic cation transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 5
Alternative name(s):
CT1
High-affinity sodium-dependent carnitine cotransporter
Organic cation/carnitine transporter 2
UST2r
Gene namesi
Name:Slc22a5
Synonyms:Octn2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 10

Organism-specific databases

RGDi3702. Slc22a5.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 2020CytoplasmicSequence analysisAdd
BLAST
Transmembranei21 – 4121Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini42 – 142101ExtracellularSequence analysisAdd
BLAST
Transmembranei143 – 16321Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini164 – 1729CytoplasmicSequence analysis
Transmembranei173 – 19321Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini194 – 1974ExtracellularSequence analysis
Transmembranei198 – 21821Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini219 – 23214CytoplasmicSequence analysisAdd
BLAST
Transmembranei233 – 25321Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini254 – 2574ExtracellularSequence analysis
Transmembranei258 – 27821Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini279 – 34163CytoplasmicSequence analysisAdd
BLAST
Transmembranei342 – 36221Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini363 – 37311ExtracellularSequence analysisAdd
BLAST
Transmembranei374 – 39421Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini395 – 40612CytoplasmicSequence analysisAdd
BLAST
Transmembranei407 – 42721Helical; Name=9Sequence analysisAdd
BLAST
Topological domaini428 – 4303ExtracellularSequence analysis
Transmembranei431 – 45121Helical; Name=10Sequence analysisAdd
BLAST
Topological domaini452 – 46211CytoplasmicSequence analysisAdd
BLAST
Transmembranei463 – 48321Helical; Name=11Sequence analysisAdd
BLAST
Topological domaini484 – 4885ExtracellularSequence analysis
Transmembranei489 – 50921Helical; Name=12Sequence analysisAdd
BLAST
Topological domaini510 – 55748CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • basolateral plasma membrane Source: RGD
  • integral component of membrane Source: RGD
  • integral component of plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1075238.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 557557Solute carrier family 22 member 5PRO_0000220502Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...)Sequence analysis
Glycosylationi64 – 641N-linked (GlcNAc...)Sequence analysis
Glycosylationi91 – 911N-linked (GlcNAc...)Sequence analysis
Modified residuei486 – 4861PhosphotyrosineBy similarity
Modified residuei548 – 5481PhosphoserineCombined sources
Modified residuei550 – 5501PhosphothreonineBy similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiO70594.
PRIDEiO70594.

PTM databases

iPTMnetiO70594.

Expressioni

Tissue specificityi

Expressed in the proximal and distal tubules and in the glomeruli in the kidney, in the myocardium, valves, and arterioles in the heart, in the labyrinthine layer of the placenta, and in the cortex, hippocampus, and cerebellum in the brain.

Gene expression databases

ExpressionAtlasiO70594. baseline and differential.
GenevisibleiO70594. RN.

Interactioni

Subunit structurei

Interacts with PDZK1.By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Cav1P413502EBI-9846085,EBI-1161014

Protein-protein interaction databases

IntActiO70594. 4 interactions.
STRINGi10116.ENSRNOP00000011340.

Chemistry

BindingDBiO70594.

Structurei

3D structure databases

ProteinModelPortaliO70594.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IRIE. Eukaryota.
ENOG410XSRI. LUCA.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234570.
HOVERGENiHBG061545.
InParanoidiO70594.
KOiK08202.
OMAiTRNIRMI.
OrthoDBiEOG7C8GH9.
PhylomeDBiO70594.
TreeFamiTF315847.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR004749. Orgcat_transp/SVOP.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70594-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDYDEVTAF LGEWGPFQRL IFFLLSASII PNGFNGMSIV FLAGTPEHRC
60 70 80 90 100
LVPHTVNLSS AWRNHSIPLE TKDGRQVPQS CRRYRLATIA NFSALGLEPG
110 120 130 140 150
RDVDLEQLEQ ENCLDGWEYN KDVFLSTIVT EWDLVCKDDW KAPLTTSLFF
160 170 180 190 200
VGVLMGSFIS GQLSDRFGRK NVLFLTMGMQ TGFSFLQLFS VNFEMFTVLF
210 220 230 240 250
VLVGMGQISN YVAAFVLGTE ILSKSIRIIF ATLGVCIFYA FGFMVLPLFA
260 270 280 290 300
YFIRDWRMLL LALTVPGVLC GALWWFIPES PRWLISQGRV KEAEVIIRKA
310 320 330 340 350
AKFNGIVAPS TIFDPSELQD LNSKKPQSHH IYDLVRTRNI RIITIMSIIL
360 370 380 390 400
WLTISVGYFG LSLDTPNLHG DIYVNCFLLA AVEVPAYVLA WLLLQHLPRR
410 420 430 440 450
YSISAALFLG GSVLLFIQLV PSELFYLSTA LVMVGKFGIT SAYSMVYVYT
460 470 480 490 500
AELYPTVVRN MGVGVSSTAS RLGSILSPYF VYLGAYDRFL PYILMGSLTI
510 520 530 540 550
LTAILTLFFP ESFGAPLPDT IDQMLRVKGI KQWQIQSQTR TQKDGGESPT

VLKSTAF
Length:557
Mass (Da):62,567
Last modified:August 1, 1998 - v1
Checksum:i172472E7B0A5F043
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti275 – 2751W → G in BAA34399 (PubMed:9792817).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001933 mRNA. Translation: CAA05106.1.
AB017260 mRNA. Translation: BAA34399.1.
AF110416 mRNA. Translation: AAD54059.1.
PIRiJE0346.
RefSeqiNP_062142.1. NM_019269.1.
UniGeneiRn.8844.

Genome annotation databases

EnsembliENSRNOT00000011340; ENSRNOP00000011340; ENSRNOG00000008432.
GeneIDi29726.
KEGGirno:29726.
UCSCiRGD:3702. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ001933 mRNA. Translation: CAA05106.1.
AB017260 mRNA. Translation: BAA34399.1.
AF110416 mRNA. Translation: AAD54059.1.
PIRiJE0346.
RefSeqiNP_062142.1. NM_019269.1.
UniGeneiRn.8844.

3D structure databases

ProteinModelPortaliO70594.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70594. 4 interactions.
STRINGi10116.ENSRNOP00000011340.

Chemistry

BindingDBiO70594.
ChEMBLiCHEMBL1075238.

PTM databases

iPTMnetiO70594.

Proteomic databases

PaxDbiO70594.
PRIDEiO70594.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000011340; ENSRNOP00000011340; ENSRNOG00000008432.
GeneIDi29726.
KEGGirno:29726.
UCSCiRGD:3702. rat.

Organism-specific databases

CTDi6584.
RGDi3702. Slc22a5.

Phylogenomic databases

eggNOGiENOG410IRIE. Eukaryota.
ENOG410XSRI. LUCA.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234570.
HOVERGENiHBG061545.
InParanoidiO70594.
KOiK08202.
OMAiTRNIRMI.
OrthoDBiEOG7C8GH9.
PhylomeDBiO70594.
TreeFamiTF315847.

Enzyme and pathway databases

ReactomeiR-RNO-549127. Organic cation transport.

Miscellaneous databases

PROiO70594.

Gene expression databases

ExpressionAtlasiO70594. baseline and differential.
GenevisibleiO70594. RN.

Family and domain databases

InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport_like.
IPR004749. Orgcat_transp/SVOP.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of two novel transport proteins from rat kidney."
    Schoemig E., Spitzenberger F., Engelhardt M., Martel F., Oerding N., Gruendemann D.
    FEBS Lett. 425:79-86(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Kidney.
  2. "Molecular cloning and characterization of high-affinity carnitine transporter from rat intestine."
    Sekine T., Kusuhara H., Utsunomiya-Tate N., Tsuda M., Sugiyama Y., Kanai Y., Endou H.
    Biochem. Biophys. Res. Commun. 251:586-591(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Intestine.
  3. "Functional characteristics and tissue distribution pattern of organic cation transporter 2 (OCTN2), an organic cation/carnitine transporter."
    Wu X., Huang W., Prasad P.D., Seth P., Rajan D.P., Leibach F.H., Chen J., Conway S.J., Ganapathy V.
    J. Pharmacol. Exp. Ther. 290:1482-1492(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
  4. "Quantitative maps of protein phosphorylation sites across 14 different rat organs and tissues."
    Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C., Olsen J.V.
    Nat. Commun. 3:876-876(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-548, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiS22A5_RAT
AccessioniPrimary (citable) accession number: O70594
Secondary accession number(s): Q9QWL0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: August 1, 1998
Last modified: July 6, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.