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Protein

E3 ubiquitin-protein ligase Midline-1

Gene

Mid1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Has E3 ubiquitin ligase activity towards IGBP1, promoting its monoubiquitination, which results in deprotection of the catalytic subunit of protein phosphatase PP2A, and its subsequent degradation by polyubiquitination.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi119 – 1191Zinc 1By similarity
Metal bindingi122 – 1221Zinc 1By similarity
Metal bindingi134 – 1341Zinc 2By similarity
Metal bindingi137 – 1371Zinc 2By similarity
Metal bindingi142 – 1421Zinc 1By similarity
Metal bindingi145 – 1451Zinc 1By similarity
Metal bindingi150 – 1501Zinc 2By similarity
Metal bindingi159 – 1591Zinc 2By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri10 – 6051RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri116 – 16550B box-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri172 – 21241B box-type 2PROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase Midline-1 (EC:6.3.2.-)
Alternative name(s):
Midin
RING finger protein Midline-1
Tripartite motif-containing protein 18
Gene namesi
Name:Mid1
Synonyms:Fxy, Trim18
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1100537. Mid1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • cytoplasmic microtubule Source: Ensembl
  • microtubule cytoskeleton Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 680680E3 ubiquitin-protein ligase Midline-1PRO_0000056228Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei92 – 921PhosphoserineBy similarity

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO70583.
PaxDbiO70583.
PRIDEiO70583.

PTM databases

iPTMnetiO70583.
PhosphoSiteiO70583.

Expressioni

Tissue specificityi

Ubiquitously expressed in fetus and adult. At E9-E10.5, highest expression found in frontonasal processes, branchial arches and CNS. From E12.5 to E16.5, high levels found in rostral part of CNS. At E14.5, begins to be highly expressed in kidney and lung. At E16.5, highly expressed in the mucosa of the hindgut and cutaneous region of the stomach.1 Publication

Developmental stagei

Expressed throughout embryonic development with highest levels from E7-E11. Also expressed in the adult.1 Publication

Gene expression databases

BgeeiO70583.
CleanExiMM_MID1.
ExpressionAtlasiO70583. baseline and differential.
GenevisibleiO70583. MM.

Interactioni

Subunit structurei

Homodimer or heterodimer with MID2. Interacts with IGBP1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Mid1ip1Q9CQ208EBI-472994,EBI-473024

Protein-protein interaction databases

BioGridi201417. 2 interactions.
IntActiO70583. 2 interactions.
MINTiMINT-8293222.
STRINGi10090.ENSMUSP00000038765.

Structurei

3D structure databases

ProteinModelPortaliO70583.
SMRiO70583. Positions 1-343, 376-668.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini320 – 37960COSPROSITE-ProRule annotationAdd
BLAST
Domaini384 – 494111Fibronectin type-IIIPROSITE-ProRule annotationAdd
BLAST
Domaini495 – 672178B30.2/SPRYPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili205 – 26460Sequence analysisAdd
BLAST

Sequence similaritiesi

Belongs to the TRIM/RBCC family.Curated
Contains 2 B box-type zinc fingers.PROSITE-ProRule annotation
Contains 1 B30.2/SPRY domain.PROSITE-ProRule annotation
Contains 1 COS domain.PROSITE-ProRule annotation
Contains 1 fibronectin type-III domain.PROSITE-ProRule annotation
Contains 1 RING-type zinc finger.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri10 – 6051RING-typePROSITE-ProRule annotationAdd
BLAST
Zinc fingeri116 – 16550B box-type 1PROSITE-ProRule annotationAdd
BLAST
Zinc fingeri172 – 21241B box-type 2PROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Coiled coil, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
GeneTreeiENSGT00760000118878.
HOGENOMiHOG000049193.
HOVERGENiHBG056432.
InParanoidiO70583.
KOiK08285.
OMAiRERAFDS.
OrthoDBiEOG7MH0XH.
TreeFamiTF333654.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003649. Bbox_C.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR017903. COS_domain.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR027727. MID1.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR24103:SF26. PTHR24103:SF26. 3 hits.
PfamiPF00041. fn3. 1 hit.
PF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00060. FN3. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS51262. COS. 1 hit.
PS50853. FN3. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O70583-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
METLESELTC PICLELFEDP LLLPCAHSLC FNCAHRILVS HCATNEPVES
60 70 80 90 100
INAFQCPTCR HVITLSQRGL DGLKRNVTLQ NIIDRFQKAS VSGPNSPSET
110 120 130 140 150
RRERAFDANT MSSAEKVLCQ FCDQDPAQDA VKTCVTCEVS YCDECLKATH
160 170 180 190 200
PNKKPFTGHR LIEPIPDSHI RGLMCLEHED EKVNMYCVTD DQLICALCKL
210 220 230 240 250
VGRHRDHQVA ALSERYDKLK QNLESNLTNL IKRNTELETL LAKLIQTCQH
260 270 280 290 300
VEVNASRQEA KLTEECDLLI EIIQQRRQII GTKIKEGKVI RLRKLAQQIA
310 320 330 340 350
NCKQCLERSA SLISQAEHSL KENDHARFLQ TAKNITERVS MATASSQVLI
360 370 380 390 400
PEINLNDTFD TFALDFSREK KLLECLDYLT APNPPAIREE LCTASYDTIT
410 420 430 440 450
VHWTSEDEFS VVSYELQYTI FTGQANVVNV ACDGTCLLGS AGLCNSADSW
460 470 480 490 500
MIVPNIKQNH YTVHGLQSGT KYIFTVKAIN QAGSRSSEPG KLKTNSQPFR
510 520 530 540 550
LDPKSAHRKL KVSHDNLTVE RDESSSKKSH APERFAGQGS YGVAGNVFID
560 570 580 590 600
SGRHYWEVVT SGSTWYAIGL AYRSAPKHEW IGKNAASWAL CRCHNHWAVR
610 620 630 640 650
HDGKETPIAP APHLRRVGVL LDYDNGSIAF YDALSSVHLH TFHAALAQPV
660 670 680
CPTFTVWNKC LTIVTGLPIP DHLDCTEQRP
Length:680
Mass (Da):76,136
Last modified:July 27, 2011 - v2
Checksum:i47D8551531157308
GO
Isoform 2 (identifier: O70583-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     429-442: NVACDGTCLLGSAG → S

Show »
Length:667
Mass (Da):74,960
Checksum:i0844933089B553D3
GO
Isoform 3 (identifier: O70583-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     183-220: Missing.
     429-442: NVACDGTCLLGSAG → S

Note: No experimental confirmation available.
Show »
Length:629
Mass (Da):70,572
Checksum:i1C4C2BD6B092B25E
GO

Sequence cautioni

The sequence AAH53704.1 differs from that shown. Reason: Erroneous termination at position 497. Translated as Gln.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti65 – 651L → P in AAH53704 (PubMed:15489334).Curated
Sequence conflicti109 – 1091N → D in AAH53704 (PubMed:15489334).Curated
Sequence conflicti174 – 1741M → T in AAB83986 (PubMed:9342357).Curated
Sequence conflicti464 – 4641H → Y in AAH53704 (PubMed:15489334).Curated
Sequence conflicti523 – 5231E → D in CAA75113 (PubMed:9467009).Curated
Sequence conflicti633 – 6331A → T in AAB83986 (PubMed:9342357).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei183 – 22038Missing in isoform 3. 1 PublicationVSP_010811Add
BLAST
Alternative sequencei429 – 44214NVACD…LGSAG → S in isoform 2 and isoform 3. 3 PublicationsVSP_005736Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14848 mRNA. Translation: CAA75113.1.
AF026565 mRNA. Translation: AAB83986.1.
AL672015, CR478112 Genomic DNA. Translation: CAM15638.1.
CR478112, AL672015 Genomic DNA. Translation: CAM28195.1.
BC053704 mRNA. Translation: AAH53704.1. Sequence problems.
CCDSiCCDS41215.1. [O70583-1]
PIRiT09013.
RefSeqiNP_001277433.1. NM_001290504.1. [O70583-2]
NP_001277434.1. NM_001290505.1. [O70583-2]
NP_001277435.1. NM_001290506.1. [O70583-3]
NP_034927.2. NM_010797.3. [O70583-1]
XP_011246091.1. XM_011247789.1. [O70583-2]
UniGeneiMm.34441.

Genome annotation databases

EnsembliENSMUST00000036753; ENSMUSP00000038765; ENSMUSG00000035299. [O70583-1]
ENSMUST00000112104; ENSMUSP00000107732; ENSMUSG00000035299. [O70583-1]
ENSMUST00000112105; ENSMUSP00000107733; ENSMUSG00000035299. [O70583-1]
ENSMUST00000163810; ENSMUSP00000128176; ENSMUSG00000035299. [O70583-1]
ENSMUST00000171433; ENSMUSP00000126746; ENSMUSG00000035299. [O70583-1]
GeneIDi17318.
KEGGimmu:17318.
UCSCiuc009uya.2. mouse. [O70583-1]
uc009uyc.2. mouse. [O70583-2]
uc057ats.1. mouse. [O70583-3]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y14848 mRNA. Translation: CAA75113.1.
AF026565 mRNA. Translation: AAB83986.1.
AL672015, CR478112 Genomic DNA. Translation: CAM15638.1.
CR478112, AL672015 Genomic DNA. Translation: CAM28195.1.
BC053704 mRNA. Translation: AAH53704.1. Sequence problems.
CCDSiCCDS41215.1. [O70583-1]
PIRiT09013.
RefSeqiNP_001277433.1. NM_001290504.1. [O70583-2]
NP_001277434.1. NM_001290505.1. [O70583-2]
NP_001277435.1. NM_001290506.1. [O70583-3]
NP_034927.2. NM_010797.3. [O70583-1]
XP_011246091.1. XM_011247789.1. [O70583-2]
UniGeneiMm.34441.

3D structure databases

ProteinModelPortaliO70583.
SMRiO70583. Positions 1-343, 376-668.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi201417. 2 interactions.
IntActiO70583. 2 interactions.
MINTiMINT-8293222.
STRINGi10090.ENSMUSP00000038765.

PTM databases

iPTMnetiO70583.
PhosphoSiteiO70583.

Proteomic databases

MaxQBiO70583.
PaxDbiO70583.
PRIDEiO70583.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000036753; ENSMUSP00000038765; ENSMUSG00000035299. [O70583-1]
ENSMUST00000112104; ENSMUSP00000107732; ENSMUSG00000035299. [O70583-1]
ENSMUST00000112105; ENSMUSP00000107733; ENSMUSG00000035299. [O70583-1]
ENSMUST00000163810; ENSMUSP00000128176; ENSMUSG00000035299. [O70583-1]
ENSMUST00000171433; ENSMUSP00000126746; ENSMUSG00000035299. [O70583-1]
GeneIDi17318.
KEGGimmu:17318.
UCSCiuc009uya.2. mouse. [O70583-1]
uc009uyc.2. mouse. [O70583-2]
uc057ats.1. mouse. [O70583-3]

Organism-specific databases

CTDi4281.
MGIiMGI:1100537. Mid1.

Phylogenomic databases

eggNOGiKOG2177. Eukaryota.
ENOG4111G04. LUCA.
GeneTreeiENSGT00760000118878.
HOGENOMiHOG000049193.
HOVERGENiHBG056432.
InParanoidiO70583.
KOiK08285.
OMAiRERAFDS.
OrthoDBiEOG7MH0XH.
TreeFamiTF333654.

Miscellaneous databases

ChiTaRSiMid1. mouse.
PROiO70583.
SOURCEiSearch...

Gene expression databases

BgeeiO70583.
CleanExiMM_MID1.
ExpressionAtlasiO70583. baseline and differential.
GenevisibleiO70583. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
3.30.40.10. 1 hit.
4.10.45.10. 1 hit.
InterProiIPR001870. B30.2/SPRY.
IPR003649. Bbox_C.
IPR003879. Butyrophylin.
IPR013320. ConA-like_dom.
IPR017903. COS_domain.
IPR003961. FN3_dom.
IPR013783. Ig-like_fold.
IPR027727. MID1.
IPR006574. PRY.
IPR003877. SPRY_dom.
IPR027370. Znf-RING_LisH.
IPR000315. Znf_B-box.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
[Graphical view]
PANTHERiPTHR24103:SF26. PTHR24103:SF26. 3 hits.
PfamiPF00041. fn3. 1 hit.
PF13765. PRY. 1 hit.
PF00622. SPRY. 1 hit.
PF00643. zf-B_box. 1 hit.
PF13445. zf-RING_UBOX. 1 hit.
[Graphical view]
PRINTSiPR01407. BUTYPHLNCDUF.
SMARTiSM00502. BBC. 1 hit.
SM00336. BBOX. 2 hits.
SM00060. FN3. 1 hit.
SM00184. RING. 1 hit.
SM00449. SPRY. 1 hit.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 1 hit.
SSF49899. SSF49899. 1 hit.
PROSITEiPS50188. B302_SPRY. 1 hit.
PS51262. COS. 1 hit.
PS50853. FN3. 1 hit.
PS50119. ZF_BBOX. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The mouse Mid1 gene: implications for the pathogenesis of Opitz syndrome and the evolution of the mammalian pseudoautosomal region."
    Zotto L.D., Quaderi N.A., Elliott R., Lingerfelter P.A., Carrel L., Valsecchi V., Montini E., Yen C.-H., Chapman V., Kalcheva I., Arrigo G., Zuffardi O., Thomas S., Willard H.F., Ballabio A., Disteche C.M., Rugarli E.I.
    Hum. Mol. Genet. 7:489-499(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Tissue: Embryo.
  2. Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2).
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
    Strain: C3H/He.
    Tissue: Osteoblast.
  5. "Characterization and physical mapping in human and mouse of a novel RING finger gene in Xp22."
    Van den Veyver I.B., Cormier T.A., Jurecic V., Baldini A., Zoghbi H.Y.
    Genomics 51:251-261(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  6. "Phosphorylation and microtubule association of the Opitz syndrome protein mid-1 is regulated by protein phosphatase 2A via binding to the regulatory subunit alpha 4."
    Liu J., Prickett T.D., Elliott E., Meroni G., Brautigan D.L.
    Proc. Natl. Acad. Sci. U.S.A. 98:6650-6655(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION.

Entry informationi

Entry nameiTRI18_MOUSE
AccessioniPrimary (citable) accession number: O70583
Secondary accession number(s): B1AV00, O35418, Q7TPT6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: August 30, 2002
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 155 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.