O70582 (LX12B_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Arachidonate 12-lipoxygenase, 12R-type
Short name=12R-LOX
Short name=12R-lipoxygenase
EC=1.13.11.-

Alternative name(s):
Epidermis-type lipoxygenase 12
Epidermis-type lipoxygenase 2
Short name=e-LOX 2

Gene names

Name:	Alox12b
Synonyms:	Aloxe2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	701 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Converts arachidonic acid to 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE).
Cofactor	Binds 1 iron ion per subunit By similarity.
Pathway	Lipid metabolism; leukotriene D4 biosynthesis.
Tissue specificity	Expressed in skin epidermis and other stratified epithelia including tongue and forestomach. Low levels of expression are found in trachea, brain and lung. Not expressed in intestine, liver, kidney, adipose tissue, muscle or hematopoietic cells. Ref.1
Developmental stage	In the embryo, expression begins at day 15.5. Ref.2
Sequence similarities	Belongs to the lipoxygenase family. Contains 1 lipoxygenase domain. Contains 1 PLAT domain.

Ontologies

Keywords
Biological process	Leukotriene biosynthesis
Coding sequence diversity	Polymorphism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	leukotriene biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	arachidonate 12-lipoxygenase activity Inferred from direct assay. Source: MGI iron ion binding Inferred from electronic annotation. Source: InterPro lipoxygenase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 701	701	Arachidonate 12-lipoxygenase, 12R-type		PRO_0000220690
Regions
	Domain	2 – 119	118	PLAT
	Domain	120 – 701	582	Lipoxygenase
Sites
	Metal binding	398	1	Iron; catalytic By similarity
	Metal binding	403	1	Iron; catalytic By similarity
	Metal binding	578	1	Iron; catalytic By similarity
	Metal binding	582	1	Iron; catalytic By similarity
	Metal binding	701	1	Iron; via carboxylate; catalytic By similarity
Natural variations
	Natural variant	9	1	A → V in strain: C57BL/6 X SJL.
	Natural variant	351	1	M → V in strain: C57BL/6 X SJL.
	Natural variant	361	1	T → I in strain: C57BL/6 X SJL.

Sequences

	Sequence		Length	Mass (Da)
	O70582 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: FAE3A3B8D2AA142E	FASTA	701	80,578
	10 20 30 40 50 60 MATYKVKVAT GTDFFSGTLD SISLTIVGTQ GESHKQRLNH FGRDFATGAV DDYTVQCQQD 70 80 90 100 110 120 LGELIIIRLH KEPHSFLAKD PWYCNYVQIC APDCRVYHFP AYQWMDGYET LALREATGKI 130 140 150 160 170 180 TADDTLPILL EHRQEEIRAK KDFYHWRVFV PGLPNYVDIP SYHPPPRRCR NPNRPEWDGY 190 200 210 220 230 240 IPGFPILINI KATRFLNSNL RFSFVKTASF FYRLGPMALA FKLRGLVDRK RSWKRLKDIK 250 260 270 280 290 300 NIFPATKSVV SEYVAEHWTE DSFFGYQYLN GINPGLIRRC TQIPDKFPVT DEMVAPFLGE 310 320 330 340 350 360 GTCLQAELER GNIYLADYRI LDGIPTVELN GQQQHHCAPM CLLHFGPDGN MMPIAIQLSQ 370 380 390 400 410 420 TPGPDCPIFL PNDSEWDWLL AKTWVRYAEF YSHEAVAHLL ESHLIGEAFC LALLRNLPMC 430 440 450 460 470 480 HPLYKLLIPH TRYNVQINSI GRALLLNKGG LSARAMSLGL EGFAQVMVRG LSELTYKSLC 490 500 510 520 530 540 IPNDFVERGV QDLPGYYFRD DSLAVWYAME RYVTEIITYY YPNDAAVEGD PELQCWVQEI 550 560 570 580 590 600 FKECLLGRES SGFPTCLRTI PELIEYVTMV MYTCSARHAA VNSGQLEYTS WMPNFPSSMR 610 620 630 640 650 660 NPPMQTKGLT TLQTYMDTLP DVKTTCIVLL VLWTLCREPD DRRPLGHFPD IHFVEEGPRR 670 680 690 700 SIEAFRQNLN QISHNIRQRN KCLTLPYYYL DPVLIENSIS I

References

[1]	"cDNA cloning of a 8-lipoxygenase and a novel epidermis-type lipoxygenase from phorbol ester-treated mouse skin." Krieg P., Kinzig A., Heidt M., Marks F., Fuerstenberger G. Biochim. Biophys. Acta 1391:7-12(1998) [PubMed: 9518531] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY. Strain: NMRI. Tissue: Epidermis.
[2]	"Human 12(R)-lipoxygenase and the mouse ortholog. Molecular cloning, expression, and gene chromosomal assignment." Sun D., McDonnell M., Chen X.-S., Lakkis M.M., Li H., Isaacs S.N., Elsea S.H., Patel P.I., Funk C.D. J. Biol. Chem. 273:33540-33547(1998) [PubMed: 9837935] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE. Strain: C57BL/6 X SJL.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y14334 mRNA. Translation: CAA74714.1. AF059251 mRNA. Translation: AAC79681.1.
IPI	IPI00119488.
RefSeq	NP_033789.1. NM_009659.2.
UniGene	Mm.340329.
3D structure databases
ProteinModelPortal	O70582.
SMR	O70582. Positions 2-701.
ModBase	Search...
Protein-protein interaction databases
STRING	O70582.
PTM databases
PhosphoSite	O70582.
Proteomic databases
PRIDE	O70582.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000036424; ENSMUSP00000035250; ENSMUSG00000032807.
GeneID	11686.
KEGG	mmu:11686.
Organism-specific databases
CTD	242.
MGI	MGI:1274782. Alox12b.
Phylogenomic databases
eggNOG	roNOG13351.
HOGENOM	HBG443995.
HOVERGEN	HBG005150.
InParanoid	O70582.
OMA	YVHIPSY.
OrthoDB	EOG48SGSH.
PhylomeDB	O70582.
Gene expression databases
ArrayExpress	O70582.
Bgee	O70582.
CleanEx	MM_ALOX12B.
Genevestigator	O70582.
GermOnline	ENSMUSG00000032807. Mus musculus.
Family and domain databases
InterPro	IPR008976. Lipase_LipOase. IPR000907. LipOase. IPR013819. LipOase_C. IPR020834. LipOase_CS. IPR020833. LipOase_Fe_BS. IPR001024. LipOase_LH2. IPR001885. LipOase_mml. [Graphical view]
Gene3D	G3DSA:2.60.60.20. Lipase_LipOase. 1 hit.
KO	K08021.
PANTHER	PTHR11771. LipOase. 1 hit.
Pfam	PF00305. Lipoxygenase. 1 hit. PF01477. PLAT. 1 hit. [Graphical view]
PRINTS	PR00087. LIPOXYGENASE. PR00467. MAMLPOXGNASE.
SMART	SM00308. LH2. 1 hit. [Graphical view]
SUPFAM	SSF49723. Lipase_LipOase. 1 hit. SSF48484. Lipoxygenase. 1 hit.
PROSITE	PS00711. LIPOXYGENASE_1. 1 hit. PS00081. LIPOXYGENASE_2. 1 hit. PS51393. LIPOXYGENASE_3. 1 hit. PS50095. PLAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	279327.
SOURCE	Search...

Entry information

Entry name

LX12B_MOUSE

Accession

Primary (citable) accession number: O70582

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 11, 2001
Last sequence update:	August 1, 1998
Last modified:	November 16, 2011
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program