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O70577 (S22A2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 110. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Solute carrier family 22 member 2
Alternative name(s):
Organic cation transporter 2
Gene names
Name:Slc22a2
Synonyms:Oct2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length553 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Mediates tubular uptake of organic compounds from circulation. Mediates the influx of agmatine, dopamine, noradrenaline (norepinephrine), serotonin, choline, famotidine, ranitidine, histamin, creatinine, amantadine, memantine, acriflavine, 4-[4-(dimethylamino)-styryl]-N-methylpyridinium ASP, amiloride, metformin, N-1-methylnicotinamide (NMN), tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, cisplatin and oxaliplatin. Cisplatin may develop a nephrotoxic action. Transport of creatinine is inhibited by fluoroquinolones such as DX-619 and LVFX. This transporter is a major determinant of the anticancer activity of oxaliplatin and may contribute to antitumor specificity By similarity.

Subcellular location

Membrane; Multi-pass membrane protein Potential.

Tissue specificity

Expressed in kidney and ureter. To a lower extent, also expressed in brain and embryo. Ref.1

Sequence similarities

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O70577-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O70577-2)

The sequence of this isoform differs from the canonical sequence as follows:
     534-544: RPRKKKEKRIY → SLGRLVQTVCH
     545-553: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 553553Solute carrier family 22 member 2
PRO_0000320958

Regions

Topological domain1 – 2121Cytoplasmic Potential
Transmembrane22 – 4221Helical; Potential
Topological domain43 – 150108Extracellular Potential
Transmembrane151 – 17121Helical; Potential
Topological domain172 – 1776Cytoplasmic Potential
Transmembrane178 – 19821Helical; Potential
Topological domain199 – 21012Extracellular Potential
Transmembrane211 – 23121Helical; Potential
Topological domain232 – 2387Cytoplasmic Potential
Transmembrane239 – 25921Helical; Potential
Topological domain260 – 2634Extracellular Potential
Transmembrane264 – 28421Helical; Potential
Topological domain285 – 34864Cytoplasmic Potential
Transmembrane349 – 36921Helical; Potential
Topological domain370 – 3756Extracellular Potential
Transmembrane376 – 39621Helical; Potential
Topological domain397 – 4048Cytoplasmic Potential
Transmembrane405 – 42521Helical; Potential
Topological domain426 – 4327Extracellular Potential
Transmembrane433 – 45321Helical; Potential
Topological domain454 – 46411Cytoplasmic Potential
Transmembrane465 – 48521Helical; Potential
Topological domain486 – 4949Extracellular Potential
Transmembrane495 – 51521Helical; Potential
Topological domain516 – 55338Cytoplasmic Potential

Sites

Site4511Involved in recognition of organic cations and participates in structural changes that occur during translocation of organic cations By similarity

Amino acid modifications

Glycosylation711N-linked (GlcNAc...) Potential

Natural variations

Alternative sequence534 – 54411RPRKKKEKRIY → SLGRLVQTVCH in isoform 2.
VSP_031775
Alternative sequence545 – 5539Missing in isoform 2.
VSP_031776

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 1E9744F0D5415483

FASTA55361,831
        10         20         30         40         50         60 
MPTVDDILEH IGEFHLFQKQ TFFLLALLSG AFTPIYVGIV FLGFTPNHHC RSPGVAELSQ 

        70         80         90        100        110        120 
RCGWSPAEEL NYTVPGLGSA GEVSFLSQCM RYEVDWNQST LDCVDPLSSL AANRSHLPLS 

       130        140        150        160        170        180 
PCEHGWVYDT PGSSIVTEFN LVCAHSWMLD LFQSLVNVGF FIGAVGIGYL ADRFGRKFCL 

       190        200        210        220        230        240 
LVTILINAIS GVLMAISPNY AWMLVFRFLQ GLVSKAGWLI GYILITEFVG LGYRRTVGIC 

       250        260        270        280        290        300 
YQIAFTVGLL ILAGVAYALP NWRWLQFAVT LPNFCFLLYF WCIPESPRWL ISQNKNAKAM 

       310        320        330        340        350        360 
KIIKHIAKKN GKSVPVSLQS LTADEDTGMK LNPSFLDLVR TPQIRKHTLI LMYNWFTSSV 

       370        380        390        400        410        420 
LYQGLIMHMG LAGDNIYLDF FYSALVEFPA AFIIILTIDR IGRRYPWAVS NMVAGAACLA 

       430        440        450        460        470        480 
SVFIPDDLQW LKITVACLGR MGITIAYEMV CLVNAELYPT YIRNLAVLVC SSMCDIGGIV 

       490        500        510        520        530        540 
TPFLVYRLTD IWLEFPLVVF AVVGLVAGGL VLLLPETKGK ALPETIEDAE KMQRPRKKKE 

       550 
KRIYLQVKKA ELS 

« Hide

Isoform 2 [UniParc].

Checksum: 3A50BB93491211AE
Show »

FASTA54460,544

References

« Hide 'large scale' references
[1]"Cloning of the mouse organic cation transporter 2 gene, Slc22a2, from an enhancer-trap transgene integration locus."
Mooslehner K.A., Allen N.D.
Mamm. Genome 10:218-224(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
Strain: 129 and BALB/c.
Tissue: Kidney and Liver.
[2]"The transcriptional landscape of the mammalian genome."
Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. expand/collapse author list , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6J.
Tissue: Kidney.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
Strain: FVB/N.
Tissue: Kidney.
[4]"Genomic sequence analysis in the mouse T-complex region."
Brathwaite M., Waeltz P., Qian Y., Dudekula D., Schlessinger D., Nagaraja R.
Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 226-553.
Strain: 129S6/SvEvTac.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AJ006036 mRNA. Translation: CAA06827.1.
AK052658 mRNA. Translation: BAC35086.1.
BC015250 mRNA. Translation: AAH15250.1.
BC069911 mRNA. Translation: AAH69911.1.
AF481054 Genomic DNA. Translation: AAM22158.1.
RefSeqNP_038695.1. NM_013667.2.
UniGeneMm.17322.

3D structure databases

ProteinModelPortalO70577.
SMRO70577. Positions 158-519.
ModBaseSearch...
MobiDBSearch...

Chemistry

ChEMBLCHEMBL2073662.

PTM databases

PhosphoSiteO70577.

Proteomic databases

PaxDbO70577.
PRIDEO70577.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000046959; ENSMUSP00000041186; ENSMUSG00000040966. [O70577-1]
GeneID20518.
KEGGmmu:20518.
UCSCuc008akw.1. mouse. [O70577-1]

Organism-specific databases

CTD6582.
MGIMGI:1335072. Slc22a2.

Phylogenomic databases

eggNOGCOG0477.
GeneTreeENSGT00730000110561.
HOGENOMHOG000234568.
HOVERGENHBG061545.
InParanoidO70577.
KOK08199.
OMARYPWAGS.
OrthoDBEOG78PV8N.
PhylomeDBO70577.
TreeFamTF315847.

Gene expression databases

BgeeO70577.
GenevestigatorO70577.

Family and domain databases

InterProIPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR004749. Orgcat_transp.
IPR005828. Sub_transporter.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
TIGRFAMsTIGR00898. 2A0119. 1 hit.
PROSITEPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio298735.
PROO70577.
SOURCESearch...

Entry information

Entry nameS22A2_MOUSE
AccessionPrimary (citable) accession number: O70577
Secondary accession number(s): Q8BWF6, Q8K4X8
Entry history
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 110 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot