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O70577

- S22A2_MOUSE

UniProt

O70577 - S22A2_MOUSE

Protein

Solute carrier family 22 member 2

Gene

Slc22a2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Mediates tubular uptake of organic compounds from circulation. Mediates the influx of agmatine, dopamine, noradrenaline (norepinephrine), serotonin, choline, famotidine, ranitidine, histamin, creatinine, amantadine, memantine, acriflavine, 4-[4-(dimethylamino)-styryl]-N-methylpyridinium ASP, amiloride, metformin, N-1-methylnicotinamide (NMN), tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, cisplatin and oxaliplatin. Cisplatin may develop a nephrotoxic action. Transport of creatinine is inhibited by fluoroquinolones such as DX-619 and LVFX. This transporter is a major determinant of the anticancer activity of oxaliplatin and may contribute to antitumor specificity By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei451 – 4511Involved in recognition of organic cations and participates in structural changes that occur during translocation of organic cationsBy similarity

    GO - Molecular functioni

    1. choline transmembrane transporter activity Source: Ensembl
    2. organic cation transmembrane transporter activity Source: MGI
    3. quaternary ammonium group transmembrane transporter activity Source: Ensembl
    4. steroid binding Source: Ensembl

    GO - Biological processi

    1. cation transport Source: MGI
    2. histamine transport Source: Ensembl
    3. organic cation transport Source: MGI

    Keywords - Biological processi

    Ion transport, Transport

    Enzyme and pathway databases

    ReactomeiREACT_196555. Abacavir transmembrane transport.
    REACT_198610. Organic cation transport.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Solute carrier family 22 member 2
    Alternative name(s):
    Organic cation transporter 2
    Gene namesi
    Name:Slc22a2
    Synonyms:Oct2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 17

    Organism-specific databases

    MGIiMGI:1335072. Slc22a2.

    Subcellular locationi

    GO - Cellular componenti

    1. basolateral plasma membrane Source: Ensembl
    2. integral component of plasma membrane Source: MGI

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 553553Solute carrier family 22 member 2PRO_0000320958Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi71 – 711N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein

    Proteomic databases

    MaxQBiO70577.
    PaxDbiO70577.
    PRIDEiO70577.

    PTM databases

    PhosphoSiteiO70577.

    Expressioni

    Tissue specificityi

    Expressed in kidney and ureter. To a lower extent, also expressed in brain and embryo.1 Publication

    Gene expression databases

    BgeeiO70577.
    GenevestigatoriO70577.

    Structurei

    3D structure databases

    ProteinModelPortaliO70577.
    SMRiO70577. Positions 158-519.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 2121CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini43 – 150108ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini172 – 1776CytoplasmicSequence Analysis
    Topological domaini199 – 21012ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini232 – 2387CytoplasmicSequence Analysis
    Topological domaini260 – 2634ExtracellularSequence Analysis
    Topological domaini285 – 34864CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini370 – 3756ExtracellularSequence Analysis
    Topological domaini397 – 4048CytoplasmicSequence Analysis
    Topological domaini426 – 4327ExtracellularSequence Analysis
    Topological domaini454 – 46411CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini486 – 4949ExtracellularSequence Analysis
    Topological domaini516 – 55338CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei22 – 4221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei151 – 17121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei178 – 19821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei211 – 23121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei239 – 25921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei264 – 28421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei349 – 36921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei376 – 39621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei405 – 42521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei433 – 45321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei465 – 48521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei495 – 51521HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0477.
    GeneTreeiENSGT00730000110561.
    HOGENOMiHOG000234568.
    HOVERGENiHBG061545.
    InParanoidiO70577.
    KOiK08199.
    OMAiHMGLAGG.
    OrthoDBiEOG78PV8N.
    PhylomeDBiO70577.
    TreeFamiTF315847.

    Family and domain databases

    InterProiIPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    IPR005829. Sugar_transporter_CS.
    [Graphical view]
    PfamiPF00083. Sugar_tr. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    TIGRFAMsiTIGR00898. 2A0119. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 2 hits.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O70577-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MPTVDDILEH IGEFHLFQKQ TFFLLALLSG AFTPIYVGIV FLGFTPNHHC    50
    RSPGVAELSQ RCGWSPAEEL NYTVPGLGSA GEVSFLSQCM RYEVDWNQST 100
    LDCVDPLSSL AANRSHLPLS PCEHGWVYDT PGSSIVTEFN LVCAHSWMLD 150
    LFQSLVNVGF FIGAVGIGYL ADRFGRKFCL LVTILINAIS GVLMAISPNY 200
    AWMLVFRFLQ GLVSKAGWLI GYILITEFVG LGYRRTVGIC YQIAFTVGLL 250
    ILAGVAYALP NWRWLQFAVT LPNFCFLLYF WCIPESPRWL ISQNKNAKAM 300
    KIIKHIAKKN GKSVPVSLQS LTADEDTGMK LNPSFLDLVR TPQIRKHTLI 350
    LMYNWFTSSV LYQGLIMHMG LAGDNIYLDF FYSALVEFPA AFIIILTIDR 400
    IGRRYPWAVS NMVAGAACLA SVFIPDDLQW LKITVACLGR MGITIAYEMV 450
    CLVNAELYPT YIRNLAVLVC SSMCDIGGIV TPFLVYRLTD IWLEFPLVVF 500
    AVVGLVAGGL VLLLPETKGK ALPETIEDAE KMQRPRKKKE KRIYLQVKKA 550
    ELS 553
    Length:553
    Mass (Da):61,831
    Last modified:August 1, 1998 - v1
    Checksum:i1E9744F0D5415483
    GO
    Isoform 2 (identifier: O70577-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         534-544: RPRKKKEKRIY → SLGRLVQTVCH
         545-553: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:544
    Mass (Da):60,544
    Checksum:i3A50BB93491211AE
    GO

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei534 – 54411RPRKKKEKRIY → SLGRLVQTVCH in isoform 2. 1 PublicationVSP_031775Add
    BLAST
    Alternative sequencei545 – 5539Missing in isoform 2. 1 PublicationVSP_031776

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ006036 mRNA. Translation: CAA06827.1.
    AK052658 mRNA. Translation: BAC35086.1.
    BC015250 mRNA. Translation: AAH15250.1.
    BC069911 mRNA. Translation: AAH69911.1.
    AF481054 Genomic DNA. Translation: AAM22158.1.
    CCDSiCCDS28392.1. [O70577-1]
    RefSeqiNP_038695.1. NM_013667.2. [O70577-1]
    UniGeneiMm.17322.

    Genome annotation databases

    EnsembliENSMUST00000046959; ENSMUSP00000041186; ENSMUSG00000040966. [O70577-1]
    GeneIDi20518.
    KEGGimmu:20518.
    UCSCiuc008akw.1. mouse. [O70577-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AJ006036 mRNA. Translation: CAA06827.1 .
    AK052658 mRNA. Translation: BAC35086.1 .
    BC015250 mRNA. Translation: AAH15250.1 .
    BC069911 mRNA. Translation: AAH69911.1 .
    AF481054 Genomic DNA. Translation: AAM22158.1 .
    CCDSi CCDS28392.1. [O70577-1 ]
    RefSeqi NP_038695.1. NM_013667.2. [O70577-1 ]
    UniGenei Mm.17322.

    3D structure databases

    ProteinModelPortali O70577.
    SMRi O70577. Positions 158-519.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    ChEMBLi CHEMBL2073662.

    PTM databases

    PhosphoSitei O70577.

    Proteomic databases

    MaxQBi O70577.
    PaxDbi O70577.
    PRIDEi O70577.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000046959 ; ENSMUSP00000041186 ; ENSMUSG00000040966 . [O70577-1 ]
    GeneIDi 20518.
    KEGGi mmu:20518.
    UCSCi uc008akw.1. mouse. [O70577-1 ]

    Organism-specific databases

    CTDi 6582.
    MGIi MGI:1335072. Slc22a2.

    Phylogenomic databases

    eggNOGi COG0477.
    GeneTreei ENSGT00730000110561.
    HOGENOMi HOG000234568.
    HOVERGENi HBG061545.
    InParanoidi O70577.
    KOi K08199.
    OMAi HMGLAGG.
    OrthoDBi EOG78PV8N.
    PhylomeDBi O70577.
    TreeFami TF315847.

    Enzyme and pathway databases

    Reactomei REACT_196555. Abacavir transmembrane transport.
    REACT_198610. Organic cation transport.

    Miscellaneous databases

    NextBioi 298735.
    PROi O70577.
    SOURCEi Search...

    Gene expression databases

    Bgeei O70577.
    Genevestigatori O70577.

    Family and domain databases

    InterProi IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR004749. Orgcat_transp.
    IPR005828. Sub_transporter.
    IPR005829. Sugar_transporter_CS.
    [Graphical view ]
    Pfami PF00083. Sugar_tr. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    TIGRFAMsi TIGR00898. 2A0119. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    PS00216. SUGAR_TRANSPORT_1. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of the mouse organic cation transporter 2 gene, Slc22a2, from an enhancer-trap transgene integration locus."
      Mooslehner K.A., Allen N.D.
      Mamm. Genome 10:218-224(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY.
      Strain: 129 and BALB/c.
      Tissue: Kidney and Liver.
    2. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Kidney.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: FVB/N.
      Tissue: Kidney.
    4. "Genomic sequence analysis in the mouse T-complex region."
      Brathwaite M., Waeltz P., Qian Y., Dudekula D., Schlessinger D., Nagaraja R.
      Submitted (FEB-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA] OF 226-553.
      Strain: 129S6/SvEvTac.

    Entry informationi

    Entry nameiS22A2_MOUSE
    AccessioniPrimary (citable) accession number: O70577
    Secondary accession number(s): Q8BWF6, Q8K4X8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 26, 2008
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3