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Protein

Solute carrier family 22 member 2

Gene

Slc22a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Mediates tubular uptake of organic compounds from circulation. Mediates the influx of agmatine, dopamine, noradrenaline (norepinephrine), serotonin, choline, famotidine, ranitidine, histamin, creatinine, amantadine, memantine, acriflavine, 4-[4-(dimethylamino)-styryl]-N-methylpyridinium ASP, amiloride, metformin, N-1-methylnicotinamide (NMN), tetraethylammonium (TEA), 1-methyl-4-phenylpyridinium (MPP), cimetidine, cisplatin and oxaliplatin. Cisplatin may develop a nephrotoxic action. Transport of creatinine is inhibited by fluoroquinolones such as DX-619 and LVFX. This transporter is a major determinant of the anticancer activity of oxaliplatin and may contribute to antitumor specificity (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei451Involved in recognition of organic cations and participates in structural changes that occur during translocation of organic cationsBy similarity1

GO - Molecular functioni

GO - Biological processi

  • cation transport Source: MGI
  • choline transport Source: GO_Central
  • dopamine transport Source: GO_Central
  • organic cation transport Source: MGI
Complete GO annotation...

Keywords - Biological processi

Ion transport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-112311. Neurotransmitter Clearance In The Synaptic Cleft.
R-MMU-181430. Norepinephrine Neurotransmitter Release Cycle.
R-MMU-2161517. Abacavir transmembrane transport.
R-MMU-442660. Na+/Cl- dependent neurotransmitter transporters.
R-MMU-549127. Organic cation transport.

Names & Taxonomyi

Protein namesi
Recommended name:
Solute carrier family 22 member 2
Alternative name(s):
Organic cation transporter 2
Gene namesi
Name:Slc22a2
Synonyms:Oct2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1335072. Slc22a2.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
Transmembranei22 – 42HelicalSequence analysisAdd BLAST21
Topological domaini43 – 150ExtracellularSequence analysisAdd BLAST108
Transmembranei151 – 171HelicalSequence analysisAdd BLAST21
Topological domaini172 – 177CytoplasmicSequence analysis6
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 210ExtracellularSequence analysisAdd BLAST12
Transmembranei211 – 231HelicalSequence analysisAdd BLAST21
Topological domaini232 – 238CytoplasmicSequence analysis7
Transmembranei239 – 259HelicalSequence analysisAdd BLAST21
Topological domaini260 – 263ExtracellularSequence analysis4
Transmembranei264 – 284HelicalSequence analysisAdd BLAST21
Topological domaini285 – 348CytoplasmicSequence analysisAdd BLAST64
Transmembranei349 – 369HelicalSequence analysisAdd BLAST21
Topological domaini370 – 375ExtracellularSequence analysis6
Transmembranei376 – 396HelicalSequence analysisAdd BLAST21
Topological domaini397 – 404CytoplasmicSequence analysis8
Transmembranei405 – 425HelicalSequence analysisAdd BLAST21
Topological domaini426 – 432ExtracellularSequence analysis7
Transmembranei433 – 453HelicalSequence analysisAdd BLAST21
Topological domaini454 – 464CytoplasmicSequence analysisAdd BLAST11
Transmembranei465 – 485HelicalSequence analysisAdd BLAST21
Topological domaini486 – 494ExtracellularSequence analysis9
Transmembranei495 – 515HelicalSequence analysisAdd BLAST21
Topological domaini516 – 553CytoplasmicSequence analysisAdd BLAST38

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2073662.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003209581 – 553Solute carrier family 22 member 2Add BLAST553

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi71N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiO70577.
PaxDbiO70577.
PRIDEiO70577.

PTM databases

iPTMnetiO70577.
PhosphoSitePlusiO70577.

Expressioni

Tissue specificityi

Expressed in kidney and ureter. To a lower extent, also expressed in brain and embryo.1 Publication

Gene expression databases

BgeeiENSMUSG00000040966.
GenevisibleiO70577. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041186.

Chemistry databases

BindingDBiO70577.

Structurei

3D structure databases

ProteinModelPortaliO70577.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234568.
HOVERGENiHBG061545.
InParanoidiO70577.
KOiK08199.
OMAiPWAASNM.
OrthoDBiEOG091G05AC.
PhylomeDBiO70577.
TreeFamiTF315847.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004749. Orgcat_transp/SVOP.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 2 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O70577-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MPTVDDILEH IGEFHLFQKQ TFFLLALLSG AFTPIYVGIV FLGFTPNHHC
60 70 80 90 100
RSPGVAELSQ RCGWSPAEEL NYTVPGLGSA GEVSFLSQCM RYEVDWNQST
110 120 130 140 150
LDCVDPLSSL AANRSHLPLS PCEHGWVYDT PGSSIVTEFN LVCAHSWMLD
160 170 180 190 200
LFQSLVNVGF FIGAVGIGYL ADRFGRKFCL LVTILINAIS GVLMAISPNY
210 220 230 240 250
AWMLVFRFLQ GLVSKAGWLI GYILITEFVG LGYRRTVGIC YQIAFTVGLL
260 270 280 290 300
ILAGVAYALP NWRWLQFAVT LPNFCFLLYF WCIPESPRWL ISQNKNAKAM
310 320 330 340 350
KIIKHIAKKN GKSVPVSLQS LTADEDTGMK LNPSFLDLVR TPQIRKHTLI
360 370 380 390 400
LMYNWFTSSV LYQGLIMHMG LAGDNIYLDF FYSALVEFPA AFIIILTIDR
410 420 430 440 450
IGRRYPWAVS NMVAGAACLA SVFIPDDLQW LKITVACLGR MGITIAYEMV
460 470 480 490 500
CLVNAELYPT YIRNLAVLVC SSMCDIGGIV TPFLVYRLTD IWLEFPLVVF
510 520 530 540 550
AVVGLVAGGL VLLLPETKGK ALPETIEDAE KMQRPRKKKE KRIYLQVKKA

ELS
Length:553
Mass (Da):61,831
Last modified:August 1, 1998 - v1
Checksum:i1E9744F0D5415483
GO
Isoform 2 (identifier: O70577-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     534-544: RPRKKKEKRIY → SLGRLVQTVCH
     545-553: Missing.

Note: No experimental confirmation available.
Show »
Length:544
Mass (Da):60,544
Checksum:i3A50BB93491211AE
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_031775534 – 544RPRKKKEKRIY → SLGRLVQTVCH in isoform 2. 1 PublicationAdd BLAST11
Alternative sequenceiVSP_031776545 – 553Missing in isoform 2. 1 Publication9

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006036 mRNA. Translation: CAA06827.1.
AK052658 mRNA. Translation: BAC35086.1.
BC015250 mRNA. Translation: AAH15250.1.
BC069911 mRNA. Translation: AAH69911.1.
AF481054 Genomic DNA. Translation: AAM22158.1.
CCDSiCCDS28392.1. [O70577-1]
RefSeqiNP_038695.1. NM_013667.2. [O70577-1]
XP_011244498.1. XM_011246196.1. [O70577-2]
UniGeneiMm.17322.

Genome annotation databases

EnsembliENSMUST00000046959; ENSMUSP00000041186; ENSMUSG00000040966. [O70577-1]
GeneIDi20518.
KEGGimmu:20518.
UCSCiuc008akw.1. mouse. [O70577-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ006036 mRNA. Translation: CAA06827.1.
AK052658 mRNA. Translation: BAC35086.1.
BC015250 mRNA. Translation: AAH15250.1.
BC069911 mRNA. Translation: AAH69911.1.
AF481054 Genomic DNA. Translation: AAM22158.1.
CCDSiCCDS28392.1. [O70577-1]
RefSeqiNP_038695.1. NM_013667.2. [O70577-1]
XP_011244498.1. XM_011246196.1. [O70577-2]
UniGeneiMm.17322.

3D structure databases

ProteinModelPortaliO70577.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000041186.

Chemistry databases

BindingDBiO70577.
ChEMBLiCHEMBL2073662.

PTM databases

iPTMnetiO70577.
PhosphoSitePlusiO70577.

Proteomic databases

MaxQBiO70577.
PaxDbiO70577.
PRIDEiO70577.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046959; ENSMUSP00000041186; ENSMUSG00000040966. [O70577-1]
GeneIDi20518.
KEGGimmu:20518.
UCSCiuc008akw.1. mouse. [O70577-1]

Organism-specific databases

CTDi6582.
MGIiMGI:1335072. Slc22a2.

Phylogenomic databases

eggNOGiKOG0255. Eukaryota.
COG0477. LUCA.
GeneTreeiENSGT00760000118852.
HOGENOMiHOG000234568.
HOVERGENiHBG061545.
InParanoidiO70577.
KOiK08199.
OMAiPWAASNM.
OrthoDBiEOG091G05AC.
PhylomeDBiO70577.
TreeFamiTF315847.

Enzyme and pathway databases

ReactomeiR-MMU-112311. Neurotransmitter Clearance In The Synaptic Cleft.
R-MMU-181430. Norepinephrine Neurotransmitter Release Cycle.
R-MMU-2161517. Abacavir transmembrane transport.
R-MMU-442660. Na+/Cl- dependent neurotransmitter transporters.
R-MMU-549127. Organic cation transport.

Miscellaneous databases

PROiO70577.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040966.
GenevisibleiO70577. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR020846. MFS_dom.
IPR005828. MFS_sugar_transport-like.
IPR004749. Orgcat_transp/SVOP.
IPR005829. Sugar_transporter_CS.
[Graphical view]
PfamiPF00083. Sugar_tr. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
TIGRFAMsiTIGR00898. 2A0119. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
PS00216. SUGAR_TRANSPORT_1. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiS22A2_MOUSE
AccessioniPrimary (citable) accession number: O70577
Secondary accession number(s): Q8BWF6, Q8K4X8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 26, 2008
Last sequence update: August 1, 1998
Last modified: November 30, 2016
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.