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Protein

Cohesin subunit SA-3

Gene

Stag3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Meiosis specific component of cohesin complex. The cohesin complex is required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The meiosis-specific cohesin complex probably replaces mitosis specific cohesin complex when it dissociates from chromatin during prophase I.2 Publications

GO - Biological processi

  • female meiosis sister chromatid cohesion Source: MGI
  • male meiosis sister chromatid cohesion Source: MGI
  • protein localization to chromosome Source: MGI
  • synapsis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Chromosome partition, Meiosis

Enzyme and pathway databases

ReactomeiR-MMU-1221632. Meiotic synapsis.
R-MMU-1221633. Meiotic Synapsis.

Names & Taxonomyi

Protein namesi
Recommended name:
Cohesin subunit SA-3
Alternative name(s):
SCC3 homolog 3
Stromal antigen 3
Stromalin-3
Gene namesi
Name:Stag3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1355311. Stag3.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication
  • Chromosome 1 Publication
  • Chromosomecentromere 1 Publication

  • Note: Associates with chromatin. In prophase I stage of meiosis, it is found along the axial elements of synaptonemal complexes. In late-pachytene-diplotene, the bulk of protein dissociates from the chromosome arms probably because of phosphorylation by PLK1, except at centromeres, where cohesin complexes remain. It however remains chromatin associated at the centromeres up to metaphase I. During anaphase I, it probably dissociates from centromeres, allowing chromosomes segregation.

GO - Cellular componenti

  • chromosome, centromeric region Source: UniProtKB-SubCell
  • condensed nuclear chromosome Source: MGI
  • extracellular space Source: MGI
  • lateral element Source: MGI
  • male germ cell nucleus Source: MGI
  • meiotic cohesin complex Source: UniProtKB
  • nuclear meiotic cohesin complex Source: MGI
  • nucleoplasm Source: Reactome
  • synaptonemal complex Source: MGI
  • transverse filament Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Deficient mice are sterile and their fetal oocytes are arrested at early prophase I leading to oocyte depletion at 1 week of age.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 12401240Cohesin subunit SA-3PRO_0000120189Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1218 – 12181PhosphoserineCombined sources

Post-translational modificationi

Phosphorylated.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO70576.
PaxDbiO70576.
PRIDEiO70576.

PTM databases

iPTMnetiO70576.
PhosphoSiteiO70576.

Expressioni

Tissue specificityi

Testis specific.

Gene expression databases

BgeeiO70576.
CleanExiMM_STAG3.
ExpressionAtlasiO70576. baseline and differential.
GenevisibleiO70576. MM.

Interactioni

Subunit structurei

Component of the meiosis-specific cohesin complex, which also contains the SMC1 (SMC1A or SMC1B) and SMC3 heterodimer. Such complex likely contains RAD21, or the meiosis-specific related protein REC8 (By similarity). Interacts with CCDC79/TERB1; recruiting cohesin to telomeres to develop structural rigidity.By similarity2 Publications

Protein-protein interaction databases

BioGridi206141. 1 interaction.
DIPiDIP-60731N.
IntActiO70576. 4 interactions.
STRINGi10090.ENSMUSP00000040945.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini324 – 40986SCDPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1195 – 12017Poly-Glu

Sequence similaritiesi

Belongs to the SCC3 family.Curated
Contains 1 SCD (stromalin conservative) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2011. Eukaryota.
COG5537. LUCA.
GeneTreeiENSGT00390000014094.
HOGENOMiHOG000231375.
HOVERGENiHBG057636.
InParanoidiO70576.
KOiK13055.
OMAiCQYSLLY.
OrthoDBiEOG7NGQ9M.
TreeFamiTF314604.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR020839. SCD.
IPR013721. STAG.
[Graphical view]
PfamiPF08514. STAG. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51425. SCD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70576-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTLWSPSTQ HHGSSSGSES SPLQKSVRRA QMALSPCSSS ILPCDDRDSQ
60 70 80 90 100
GTAEWDSPST NEDSDFEDSL RRNVKKRAAK QPPKAVPAAK HRKKQSRIVS
110 120 130 140 150
SGNGKNESVP STNYLFDAVK AARSCMQSLV DEWLDNYKQD ENAGFLELIN
160 170 180 190 200
FFIRACGCKS TVTPEMFKTM SNSEIIQHLT EEFNEDSGDY PLTAPGPSWK
210 220 230 240 250
KFQGSFCEFV KTLVYQCQYS LLYDGFPMDD LISLLIGLSD SQVRAFRHTS
260 270 280 290 300
TLAAMKLMTS LVKVALQLSL HKDNNQRQYE AERNKGPEQR APERLESLLE
310 320 330 340 350
KRKEFQENQE DIEGMMNAIF RGVFVHRYRD ILPEIRAICI EEIGYWMQSY
360 370 380 390 400
STSFLNDSYL KYIGWTLHDK HKEVRLKCVK ALAGLYSNQE LSLRMELFTN
410 420 430 440 450
RFKDRMVSMV MDRECEVAVE AIRLLTLILK NMEGVLTSAD CEKIYSIVYI
460 470 480 490 500
SNRAMASSAG EFVYWKIFHP ECGAKAVSDR ERRRSPQAQK TFIYLLLAFF
510 520 530 540 550
MESEHHNHAA YLVDSLWDCA GSYLKDWESL TNLLLQKDQN LGDMQERMLI
560 570 580 590 600
EILVSSARQA AEGHPPVGRI TGKKSLTAKE RKLQAYDKMK LAEHLIPLLP
610 620 630 640 650
QLLAKFSADA ENVAPLLQLL SYFDLSIYCT QRLEKHLELL LQQLQEVVVK
660 670 680 690 700
HVEPEVLEAA AHALYLLCKP EFTFFSRVDF ARSQLVDFLT DRFQQELDDL
710 720 730 740 750
MQSSFLDEDE VYSLTATLKR LSAFYNAHDL TRWEISEPCS RLLRKAVDTG
760 770 780 790 800
EVPHQVILPA LTLVYFSILW TVTHISESTS HKQLMSLKKR MVAFCELCQS
810 820 830 840 850
CLSDVDPEIQ EQAFVLLSDL LLIFSPQMIV GGRDFLRPLV FFPEATLQSE
860 870 880 890 900
LASFLMDHVF LQPGELGNGQ SQEDHVQIEL LHQRRRLLAG FCKLLLYGVL
910 920 930 940 950
ELDAASDVFK HYNKFYEDYG DIIKETLTRA RQIDRCQCSR ILLLSLKQLY
960 970 980 990 1000
TELIQEQGPQ GLTELPAFIE MRDLARRFAL SFGPQQLHNR DLVVMLHKEG
1010 1020 1030 1040 1050
IKFSLSELPP AGSSHEPPNL AFLELLSEFS PRLFHQDKRL LLSYLEKCLQ
1060 1070 1080 1090 1100
RVSKAPNHPW GPVTTYCHSL HPLEITAEAS PRGPPHSKKR CVEGPCRPQE
1110 1120 1130 1140 1150
EESSSQEESL QLNSGPTTPT LTSTAVKRKQ SLRTVGKKQK GRPGPGPGPG
1160 1170 1180 1190 1200
PELICSQQLL GTQRLKMSSA PCFQIRCDPS GSGLGKQLTR LSLMEEDEEE
1210 1220 1230 1240
ELRLLDEEWQ RGDKMLHSPS SPSEHGLDLL DTTELNMEDF
Length:1,240
Mass (Da):141,167
Last modified:July 27, 2011 - v2
Checksum:iB3856012BFE76C1C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti553 – 5531L → P in CAA06669 (PubMed:10698974).Curated
Sequence conflicti604 – 6041A → D in CAA06669 (PubMed:10698974).Curated
Sequence conflicti1079 – 10791A → G in CAA06669 (PubMed:10698974).Curated
Sequence conflicti1090 – 10901R → K in CAA06669 (PubMed:10698974).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005678 mRNA. Translation: CAA06669.1.
BC139010 mRNA. Translation: AAI39011.1.
BC139011 mRNA. Translation: AAI39012.1.
CCDSiCCDS39344.1.
PIRiT30834.
RefSeqiNP_058660.2. NM_016964.2.
XP_006504659.1. XM_006504596.1.
XP_011239250.1. XM_011240948.1.
UniGeneiMm.285609.

Genome annotation databases

EnsembliENSMUST00000048028; ENSMUSP00000040945; ENSMUSG00000036928.
ENSMUST00000162245; ENSMUSP00000125523; ENSMUSG00000036928.
GeneIDi50878.
KEGGimmu:50878.
UCSCiuc009afn.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ005678 mRNA. Translation: CAA06669.1.
BC139010 mRNA. Translation: AAI39011.1.
BC139011 mRNA. Translation: AAI39012.1.
CCDSiCCDS39344.1.
PIRiT30834.
RefSeqiNP_058660.2. NM_016964.2.
XP_006504659.1. XM_006504596.1.
XP_011239250.1. XM_011240948.1.
UniGeneiMm.285609.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi206141. 1 interaction.
DIPiDIP-60731N.
IntActiO70576. 4 interactions.
STRINGi10090.ENSMUSP00000040945.

PTM databases

iPTMnetiO70576.
PhosphoSiteiO70576.

Proteomic databases

MaxQBiO70576.
PaxDbiO70576.
PRIDEiO70576.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048028; ENSMUSP00000040945; ENSMUSG00000036928.
ENSMUST00000162245; ENSMUSP00000125523; ENSMUSG00000036928.
GeneIDi50878.
KEGGimmu:50878.
UCSCiuc009afn.2. mouse.

Organism-specific databases

CTDi10734.
MGIiMGI:1355311. Stag3.

Phylogenomic databases

eggNOGiKOG2011. Eukaryota.
COG5537. LUCA.
GeneTreeiENSGT00390000014094.
HOGENOMiHOG000231375.
HOVERGENiHBG057636.
InParanoidiO70576.
KOiK13055.
OMAiCQYSLLY.
OrthoDBiEOG7NGQ9M.
TreeFamiTF314604.

Enzyme and pathway databases

ReactomeiR-MMU-1221632. Meiotic synapsis.
R-MMU-1221633. Meiotic Synapsis.

Miscellaneous databases

PROiO70576.
SOURCEiSearch...

Gene expression databases

BgeeiO70576.
CleanExiMM_STAG3.
ExpressionAtlasiO70576. baseline and differential.
GenevisibleiO70576. MM.

Family and domain databases

Gene3Di1.25.10.10. 2 hits.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR020839. SCD.
IPR013721. STAG.
[Graphical view]
PfamiPF08514. STAG. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 2 hits.
PROSITEiPS51425. SCD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "STAG3, a novel gene encoding a protein involved in meiotic chromosome pairing and location of STAG3-related genes flanking the Williams-Beuren syndrome deletion."
    Pezzi N., Prieto I., Kremer L., Perez Jurado L.A., Valero C., Del Mazo J., Martinez-A C., Barbero J.L.
    FASEB J. 14:581-592(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CHARACTERIZATION.
    Tissue: Testis.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Mammalian STAG3 is a cohesin specific to sister chromatid arms in meiosis I."
    Prieto I., Suja J.A., Pezzi N., Kremer L., Martinez-A C., Rufas J.S., Barbero J.L.
    Nat. Cell Biol. 3:761-766(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MEIOSIS, INTERACTION WITH SMC1A AND SMC3.
  4. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1218, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Testis.
  5. "Phosphorylation of chromosome core components may serve as axis marks for the status of chromosomal events during mammalian meiosis."
    Fukuda T., Pratto F., Schimenti J.C., Turner J.M., Camerini-Otero R.D., Hoeoeg C.
    PLoS Genet. 8:E1002485-E1002485(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, PHOSPHORYLATION.
  6. "The TRF1-binding protein TERB1 promotes chromosome movement and telomere rigidity in meiosis."
    Shibuya H., Ishiguro K.I., Watanabe Y.
    Nat. Cell Biol. 16:145-156(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCDC79.
  7. Cited for: DISRUPTION PHENOTYPE, FUNCTION.

Entry informationi

Entry nameiSTAG3_MOUSE
AccessioniPrimary (citable) accession number: O70576
Secondary accession number(s): B2RSV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 25, 2003
Last sequence update: July 27, 2011
Last modified: June 8, 2016
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.