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Protein

Sphingomyelin phosphodiesterase 2

Gene

Smpd2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts sphingomyelin to ceramide. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor). The physiological substrate seems to be Lyso-PAF (By similarity).By similarity

Catalytic activityi

Sphingomyelin + H2O = N-acylsphingosine + phosphocholine.

Cofactori

pH dependencei

Optimum pH is 6.5-7.5.

Pathwayi: sphingolipid metabolism

This protein is involved in the pathway sphingolipid metabolism, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway sphingolipid metabolism and in Lipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi49MagnesiumBy similarity1
Sitei180Important for substrate recognitionBy similarity1
Active sitei272Proton acceptorBy similarity1

GO - Molecular functioni

  • metal ion binding Source: UniProtKB-KW
  • sphingomyelin phosphodiesterase activity Source: MGI

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid metabolism, Sphingolipid metabolism
LigandMagnesium, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-1660662 Glycosphingolipid metabolism
R-MMU-5626978 TNFR1-mediated ceramide production
UniPathwayiUPA00222

Names & Taxonomyi

Protein namesi
Recommended name:
Sphingomyelin phosphodiesterase 2 (EC:3.1.4.12)
Alternative name(s):
Lyso-platelet-activating factor-phospholipase C
Short name:
Lyso-PAF-PLC
Neutral sphingomyelinase
Short name:
N-SMase
Short name:
nSMase
Gene namesi
Name:Smpd2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1278330 Smpd2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei326 – 346HelicalSequence analysisAdd BLAST21
Transmembranei354 – 374HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Mice lacking Smpd2 and Smpd3 are completely devoid of neutral SMase activity but do not developed sphingomyelin storage abnormalities.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000756871 – 419Sphingomyelin phosphodiesterase 2Add BLAST419

Proteomic databases

MaxQBiO70572
PaxDbiO70572
PeptideAtlasiO70572
PRIDEiO70572

PTM databases

iPTMnetiO70572
PhosphoSitePlusiO70572

Expressioni

Tissue specificityi

Although widely expressed in all tissues examined, except the spleen, high enzymatic activity occurs only in the brain.1 Publication

Gene expression databases

BgeeiENSMUSG00000019822
CleanExiMM_SMPD2
ExpressionAtlasiO70572 baseline and differential
GenevisibleiO70572 MM

Interactioni

Protein-protein interaction databases

BioGridi203346, 1 interactor
STRINGi10090.ENSMUSP00000019965

Structurei

3D structure databases

ProteinModelPortaliO70572
SMRiO70572
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the neutral sphingomyelinase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3873 Eukaryota
ENOG410ZFPY LUCA
GeneTreeiENSGT00390000009166
HOGENOMiHOG000044683
HOVERGENiHBG019089
InParanoidiO70572
KOiK12351
OMAiRIQHADW
OrthoDBiEOG091G0EZ8
PhylomeDBiO70572
TreeFamiTF313899

Family and domain databases

Gene3Di3.60.10.10, 1 hit
InterProiView protein in InterPro
IPR036691 Endo/exonu/phosph_ase_sf
IPR005135 Endo/exonuclease/phosphatase
PfamiView protein in Pfam
PF03372 Exo_endo_phos, 1 hit
SUPFAMiSSF56219 SSF56219, 1 hit

Sequencei

Sequence statusi: Complete.

O70572-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLNFSLRLR VFNLNCWDIP YLSKHRADRM KRLGDFLNLE NFDLALLEEV
60 70 80 90 100
WSEQDFQYLR QRLSLTYPDA HYFRSGMIGS GLCVFSKHPI QEIFQHVYSL
110 120 130 140 150
NGYPYMFHHG DWFCGKSVGL LVLRLSGLVL NAYVTHLHAE YSRQKDIYFA
160 170 180 190 200
HRVAQAWELA QFIHHTSKNA DVVLLCGDLN MHPKDLGCCL LKEWTGLHDA
210 220 230 240 250
FVETEDFKGS DDGCTMVPKN CYVSQQDLGP FPSGIRIDYV LYKAVSEFHV
260 270 280 290 300
CCETLKTTTG CDPHSDKPFS DHEALMATLY VKHSPPQEDP CTACGPLERS
310 320 330 340 350
DLISVLREAR TELGLGIAKA RWWAAFSGYV IVWGLSLLVL LCVLAAGEEA
360 370 380 390 400
REVAIILCIP SVGLVLVAGA VYLFHKQEAK GLCRAQAEML HVLTRETETQ
410
DRGSEPHLAY CLQQEGDRA
Length:419
Mass (Da):47,467
Last modified:August 1, 1998 - v1
Checksum:i5812FC630A69C356
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ222800 mRNA Translation: CAA10994.1
BC010978 mRNA Translation: AAH10978.1
CCDSiCCDS23805.1
RefSeqiNP_033239.1, NM_009213.2
UniGeneiMm.953

Genome annotation databases

EnsembliENSMUST00000019965; ENSMUSP00000019965; ENSMUSG00000019822
GeneIDi20598
KEGGimmu:20598
UCSCiuc007exs.1 mouse

Similar proteinsi

Entry informationi

Entry nameiNSMA_MOUSE
AccessioniPrimary (citable) accession number: O70572
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 11, 2002
Last sequence update: August 1, 1998
Last modified: April 25, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health