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Protein

Protein diaphanous homolog 2

Gene

Diaph2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in oogenesis.

GO - Molecular functioni

  • actin binding Source: MGI
  • Rho GTPase binding Source: InterPro

GO - Biological processi

  • actin filament polymerization Source: MGI
  • multicellular organism development Source: UniProtKB-KW
  • oogenesis Source: UniProtKB-KW

Keywordsi

Molecular functionDevelopmental protein
Biological processDifferentiation, Oogenesis

Enzyme and pathway databases

ReactomeiR-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein diaphanous homolog 2
Alternative name(s):
Diaphanous-related formin-2
Short name:
DRF2
Short name:
mDia3
Gene namesi
Name:Diaph2
Synonyms:Diap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1858500 Diaph2

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001948961 – 1098Protein diaphanous homolog 2Add BLAST1098

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiO70566
MaxQBiO70566
PaxDbiO70566
PRIDEiO70566

PTM databases

iPTMnetiO70566
PhosphoSitePlusiO70566

Expressioni

Developmental stagei

Expressed in liver, heart, kidney, ovary and testis, at E16, P6 and P16.

Gene expression databases

BgeeiENSMUSG00000034480
CleanExiMM_DIAP2
ExpressionAtlasiO70566 baseline and differential
GenevisibleiO70566 MM

Interactioni

Subunit structurei

Interacts with MAPRE1 and APC.1 Publication

GO - Molecular functioni

  • actin binding Source: MGI
  • Rho GTPase binding Source: InterPro

Protein-protein interaction databases

BioGridi207557, 2 interactors
IntActiO70566, 2 interactors
STRINGi10090.ENSMUSP00000039334

Structurei

3D structure databases

ProteinModelPortaliO70566
SMRiO70566
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini90 – 463GBD/FH3PROSITE-ProRule annotationAdd BLAST374
Domaini544 – 620FH1Add BLAST77
Domaini625 – 1025FH2PROSITE-ProRule annotationAdd BLAST401
Domaini1048 – 1078DADPROSITE-ProRule annotationAdd BLAST31

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili375 – 416Sequence analysisAdd BLAST42
Coiled coili490 – 539Sequence analysisAdd BLAST50
Coiled coili999 – 1050Sequence analysisAdd BLAST52

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi1035 – 1038Poly-Lys4
Compositional biasi1069 – 1072Arg/Lys-rich (basic)4

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiKOG1924 Eukaryota
ENOG410Y29H LUCA
GeneTreeiENSGT00760000118986
HOGENOMiHOG000293231
HOVERGENiHBG051357
InParanoidiO70566
PhylomeDBiO70566

Family and domain databases

Gene3Di1.25.10.10, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR014767 DAD_dom
IPR027644 DIAPH2
IPR010465 Drf_DAD
IPR015425 FH2_Formin
IPR010472 FH3_dom
IPR014768 GBD/FH3_dom
IPR010473 GTPase-bd
PANTHERiPTHR23213:SF174 PTHR23213:SF174, 1 hit
PfamiView protein in Pfam
PF06345 Drf_DAD, 1 hit
PF06367 Drf_FH3, 1 hit
PF06371 Drf_GBD, 1 hit
PF02181 FH2, 1 hit
SMARTiView protein in SMART
SM01139 Drf_FH3, 1 hit
SM01140 Drf_GBD, 1 hit
SM00498 FH2, 1 hit
SUPFAMiSSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS51231 DAD, 1 hit
PS51444 FH2, 1 hit
PS51232 GBD_FH3, 1 hit

Sequencei

Sequence statusi: Complete.

O70566-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEELGAAASG AGGGGGGGEE HGGGRSNKRG AGNRAANEEE TRNKPKLRDR
60 70 80 90 100
ITSFRKSATK REKPVIQHSI DYQTAVVEIP PALIVHDDRS LILSEKEVLD
110 120 130 140 150
LFEKMMEDMN LNEEKKAPLR KKDFSIKREM VVQYISATSK SIVGSKVLGG
160 170 180 190 200
LKNSKHEFTL SSQEYVHELR SGISDEKLLN CLESLRVSLT SHPVSWVNNF
210 220 230 240 250
GYEGLGVLLD VLEKLLDKKQ QENIDKKNQY KVIQCLKAFM NNKFGLQRIL
260 270 280 290 300
GDERSLLLLA RAIDPKQQNM MTEIVKILSA ICIVGEENIL DKLLGGITAA
310 320 330 340 350
AELNNRERFS PIVEGLENNE ALHLQVACMQ FINALVTSPY DLDFRIHLRN
360 370 380 390 400
EFLRCGLKAM LPTLKEIENE GLDIQLRVFE ENKEDDLSEL SHRLNDIRAE
410 420 430 440 450
MDDINEVYHL LYNMLKDTAA EPYLLSILQH FLLIRNDYYI RPQYYKIIEE
460 470 480 490 500
CVSQIVLHCS GMDPDFKYRQ RIDFDFTHLL DACVNKAKVE ENEQKAMEFS
510 520 530 540 550
KKFDEEFTAR QEAQAELQKR DEKIKELETE IQQLRGQGVP SAIPGPPPPP
560 570 580 590 600
PLPGAGPCPP PPPPPPPPPP LPGVVPPPPP PLPGMPGIPP PPPPPLSGVP
610 620 630 640 650
PPPPPPGGVF PLLSGPIELP YGMKQKKLYK PDIPMKRINW SKIEPKELSE
660 670 680 690 700
NCVWLKLKEE KYENADLFAK LALTFPSQMK GQRNTEAAEE NRSGPPKKKV
710 720 730 740 750
KELRILDTKT AQNLSIFLGS YRMPYEEIKN IILEVNEEML SEALIQNLVK
760 770 780 790 800
YLPDQNALRE LAQLKSEYDD LCEPEQFGVV MSTVKMLRPR LTSILFKLTF
810 820 830 840 850
EEHVNNIKPS IIAVTLACEE LKKSESFKRL LELILLVGNY MNSGSRNAQS
860 870 880 890 900
LGFKINFLCK IKDTKSADQK STLLHFLAEI CDEKYRDILK FPDELEHVES
910 920 930 940 950
AGKVSAQILK SNLVAMEQSI LHLEKNIKNF PPAESHHDKF VEKMMSFTQN
960 970 980 990 1000
AREQYDKLST MHSNMLKLYE SLGEYFIFDP NTVNMEEFFG DLNTFRTLFL
1010 1020 1030 1040 1050
EALKENHKRK EMEEKSRRAK LAKEKAEQEK LERQKKKKQL IDINKEGDET
1060 1070 1080 1090
GVMDNLLEAL QSGAAFRDRR KRIPRNPDNR RPPLERSRSR HNGAMSSK
Length:1,098
Mass (Da):124,871
Last modified:February 16, 2004 - v2
Checksum:iBB19D49E049EA8CA
GO

Sequence cautioni

The sequence BAC40476 differs from that shown. Probable cloning artifact.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti750 – 751KY → RD in CAA75871 (PubMed:9497258).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK088648 mRNA Translation: BAC40476.1 Sequence problems.
Y15910 mRNA Translation: CAA75871.1
RefSeqiXP_006528645.1, XM_006528582.3
UniGeneiMm.10763

Genome annotation databases

EnsembliENSMUST00000113320; ENSMUSP00000108946; ENSMUSG00000034480
GeneIDi54004

Similar proteinsi

Entry informationi

Entry nameiDIAP2_MOUSE
AccessioniPrimary (citable) accession number: O70566
Secondary accession number(s): Q8C2G8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 16, 2004
Last modified: March 28, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health