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O70566

- DIAP2_MOUSE

UniProt

O70566 - DIAP2_MOUSE

Protein

Protein diaphanous homolog 2

Gene

Diaph2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 113 (01 Oct 2014)
      Sequence version 2 (16 Feb 2004)
      Previous versions | rss
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    Functioni

    May be involved in oogenesis.

    GO - Molecular functioni

    1. actin binding Source: MGI

    GO - Biological processi

    1. actin filament polymerization Source: MGI
    2. multicellular organismal development Source: UniProtKB-KW
    3. oogenesis Source: UniProtKB-KW

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Differentiation, Oogenesis

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Protein diaphanous homolog 2
    Alternative name(s):
    Diaphanous-related formin-2
    Short name:
    DRF2
    Short name:
    mDia3
    Gene namesi
    Name:Diaph2
    Synonyms:Diap2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome X

    Organism-specific databases

    MGIiMGI:1858500. Diap2.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 10981098Protein diaphanous homolog 2PRO_0000194896Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiO70566.
    PaxDbiO70566.
    PRIDEiO70566.

    PTM databases

    PhosphoSiteiO70566.

    Expressioni

    Developmental stagei

    Expressed in liver, heart, kidney, ovary and testis, at E16, P6 and P16.

    Gene expression databases

    BgeeiO70566.
    CleanExiMM_DIAP2.
    GenevestigatoriO70566.

    Interactioni

    Subunit structurei

    Interacts with MAPRE1 and APC.1 Publication

    Protein-protein interaction databases

    MINTiMINT-5023280.

    Structurei

    3D structure databases

    ProteinModelPortaliO70566.
    SMRiO70566. Positions 627-1067.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini90 – 463374GBD/FH3PROSITE-ProRule annotationAdd
    BLAST
    Domaini544 – 62077FH1Add
    BLAST
    Domaini625 – 1025401FH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini1048 – 107831DADPROSITE-ProRule annotationAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili375 – 41642Sequence AnalysisAdd
    BLAST
    Coiled coili490 – 53950Sequence AnalysisAdd
    BLAST
    Coiled coili999 – 105052Sequence AnalysisAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi1035 – 10384Poly-Lys
    Compositional biasi1069 – 10724Arg/Lys-rich (basic)

    Domaini

    The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments By similarity.By similarity

    Sequence similaritiesi

    Contains 1 DAD (diaphanous autoregulatory) domain.PROSITE-ProRule annotation
    Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
    Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil, Repeat

    Phylogenomic databases

    eggNOGiNOG149898.
    GeneTreeiENSGT00730000110702.
    HOGENOMiHOG000293231.
    HOVERGENiHBG051357.
    KOiK05741.
    PhylomeDBiO70566.

    Family and domain databases

    InterProiIPR016024. ARM-type_fold.
    IPR027644. DIAPH2.
    IPR014767. Diaphanous_autoregulatory.
    IPR010465. Drf_DAD.
    IPR015425. FH2_Formin.
    IPR010472. FH3_dom.
    IPR010473. GTPase-bd.
    IPR014768. GTPase-bd/formin_homology_3.
    [Graphical view]
    PANTHERiPTHR23213:SF174. PTHR23213:SF174. 1 hit.
    PfamiPF06345. Drf_DAD. 1 hit.
    PF06367. Drf_FH3. 1 hit.
    PF06371. Drf_GBD. 1 hit.
    PF02181. FH2. 1 hit.
    [Graphical view]
    SMARTiSM00498. FH2. 1 hit.
    [Graphical view]
    SUPFAMiSSF48371. SSF48371. 1 hit.
    PROSITEiPS51231. DAD. 1 hit.
    PS51444. FH2. 1 hit.
    PS51232. GBD_FH3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O70566-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEELGAAASG AGGGGGGGEE HGGGRSNKRG AGNRAANEEE TRNKPKLRDR     50
    ITSFRKSATK REKPVIQHSI DYQTAVVEIP PALIVHDDRS LILSEKEVLD 100
    LFEKMMEDMN LNEEKKAPLR KKDFSIKREM VVQYISATSK SIVGSKVLGG 150
    LKNSKHEFTL SSQEYVHELR SGISDEKLLN CLESLRVSLT SHPVSWVNNF 200
    GYEGLGVLLD VLEKLLDKKQ QENIDKKNQY KVIQCLKAFM NNKFGLQRIL 250
    GDERSLLLLA RAIDPKQQNM MTEIVKILSA ICIVGEENIL DKLLGGITAA 300
    AELNNRERFS PIVEGLENNE ALHLQVACMQ FINALVTSPY DLDFRIHLRN 350
    EFLRCGLKAM LPTLKEIENE GLDIQLRVFE ENKEDDLSEL SHRLNDIRAE 400
    MDDINEVYHL LYNMLKDTAA EPYLLSILQH FLLIRNDYYI RPQYYKIIEE 450
    CVSQIVLHCS GMDPDFKYRQ RIDFDFTHLL DACVNKAKVE ENEQKAMEFS 500
    KKFDEEFTAR QEAQAELQKR DEKIKELETE IQQLRGQGVP SAIPGPPPPP 550
    PLPGAGPCPP PPPPPPPPPP LPGVVPPPPP PLPGMPGIPP PPPPPLSGVP 600
    PPPPPPGGVF PLLSGPIELP YGMKQKKLYK PDIPMKRINW SKIEPKELSE 650
    NCVWLKLKEE KYENADLFAK LALTFPSQMK GQRNTEAAEE NRSGPPKKKV 700
    KELRILDTKT AQNLSIFLGS YRMPYEEIKN IILEVNEEML SEALIQNLVK 750
    YLPDQNALRE LAQLKSEYDD LCEPEQFGVV MSTVKMLRPR LTSILFKLTF 800
    EEHVNNIKPS IIAVTLACEE LKKSESFKRL LELILLVGNY MNSGSRNAQS 850
    LGFKINFLCK IKDTKSADQK STLLHFLAEI CDEKYRDILK FPDELEHVES 900
    AGKVSAQILK SNLVAMEQSI LHLEKNIKNF PPAESHHDKF VEKMMSFTQN 950
    AREQYDKLST MHSNMLKLYE SLGEYFIFDP NTVNMEEFFG DLNTFRTLFL 1000
    EALKENHKRK EMEEKSRRAK LAKEKAEQEK LERQKKKKQL IDINKEGDET 1050
    GVMDNLLEAL QSGAAFRDRR KRIPRNPDNR RPPLERSRSR HNGAMSSK 1098
    Length:1,098
    Mass (Da):124,871
    Last modified:February 16, 2004 - v2
    Checksum:iBB19D49E049EA8CA
    GO

    Sequence cautioni

    The sequence BAC40476.1 differs from that shown. Reason: Probable cloning artifact.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti750 – 7512KY → RD in CAA75871. (PubMed:9497258)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK088648 mRNA. Translation: BAC40476.1. Sequence problems.
    Y15910 mRNA. Translation: CAA75871.1.
    RefSeqiXP_006528645.1. XM_006528582.1.
    UniGeneiMm.10763.

    Genome annotation databases

    EnsembliENSMUST00000113320; ENSMUSP00000108946; ENSMUSG00000034480.
    GeneIDi54004.
    KEGGimmu:54004.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK088648 mRNA. Translation: BAC40476.1 . Sequence problems.
    Y15910 mRNA. Translation: CAA75871.1 .
    RefSeqi XP_006528645.1. XM_006528582.1.
    UniGenei Mm.10763.

    3D structure databases

    ProteinModelPortali O70566.
    SMRi O70566. Positions 627-1067.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-5023280.

    PTM databases

    PhosphoSitei O70566.

    Proteomic databases

    MaxQBi O70566.
    PaxDbi O70566.
    PRIDEi O70566.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000113320 ; ENSMUSP00000108946 ; ENSMUSG00000034480 .
    GeneIDi 54004.
    KEGGi mmu:54004.

    Organism-specific databases

    CTDi 54004.
    MGIi MGI:1858500. Diap2.

    Phylogenomic databases

    eggNOGi NOG149898.
    GeneTreei ENSGT00730000110702.
    HOGENOMi HOG000293231.
    HOVERGENi HBG051357.
    KOi K05741.
    PhylomeDBi O70566.

    Miscellaneous databases

    NextBioi 13841926.
    PROi O70566.
    SOURCEi Search...

    Gene expression databases

    Bgeei O70566.
    CleanExi MM_DIAP2.
    Genevestigatori O70566.

    Family and domain databases

    InterProi IPR016024. ARM-type_fold.
    IPR027644. DIAPH2.
    IPR014767. Diaphanous_autoregulatory.
    IPR010465. Drf_DAD.
    IPR015425. FH2_Formin.
    IPR010472. FH3_dom.
    IPR010473. GTPase-bd.
    IPR014768. GTPase-bd/formin_homology_3.
    [Graphical view ]
    PANTHERi PTHR23213:SF174. PTHR23213:SF174. 1 hit.
    Pfami PF06345. Drf_DAD. 1 hit.
    PF06367. Drf_FH3. 1 hit.
    PF06371. Drf_GBD. 1 hit.
    PF02181. FH2. 1 hit.
    [Graphical view ]
    SMARTi SM00498. FH2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48371. SSF48371. 1 hit.
    PROSITEi PS51231. DAD. 1 hit.
    PS51444. FH2. 1 hit.
    PS51232. GBD_FH3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: NOD.
      Tissue: Thymus.
    2. "A human homologue of the Drosophila melanogaster diaphanous gene is disrupted in a patient with premature ovarian failure: evidence for conserved function in oogenesis and implications for human sterility."
      Bione S., Sala C., Manzini C., Arrigo G., Zuffardi O., Banfi S., Borsani G., Jonveaux P., Philippe C., Zuccotti M., Ballabio A., Toniolo D.
      Am. J. Hum. Genet. 62:533-541(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 750-1098.
      Strain: BALB/c.
    3. "EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration."
      Wen Y., Eng C.H., Schmoranzer J., Cabrera-Poch N., Morris E.J.S., Chen M., Wallar B.J., Alberts A.S., Gundersen G.G.
      Nat. Cell Biol. 6:820-830(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH APC AND MAPRE1.

    Entry informationi

    Entry nameiDIAP2_MOUSE
    AccessioniPrimary (citable) accession number: O70566
    Secondary accession number(s): Q8C2G8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: February 16, 2004
    Last modified: October 1, 2014
    This is version 113 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3