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O70566

- DIAP2_MOUSE

UniProt

O70566 - DIAP2_MOUSE

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Protein

Protein diaphanous homolog 2

Gene

Diaph2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli

Functioni

May be involved in oogenesis.

GO - Molecular functioni

  1. actin binding Source: MGI

GO - Biological processi

  1. actin filament polymerization Source: MGI
  2. multicellular organismal development Source: UniProtKB-KW
  3. oogenesis Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Differentiation, Oogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Protein diaphanous homolog 2
Alternative name(s):
Diaphanous-related formin-2
Short name:
DRF2
Short name:
mDia3
Gene namesi
Name:Diaph2
Synonyms:Diap2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome X

Organism-specific databases

MGIiMGI:1858500. Diap2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10981098Protein diaphanous homolog 2PRO_0000194896Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiO70566.
PaxDbiO70566.
PRIDEiO70566.

PTM databases

PhosphoSiteiO70566.

Expressioni

Developmental stagei

Expressed in liver, heart, kidney, ovary and testis, at E16, P6 and P16.

Gene expression databases

BgeeiO70566.
CleanExiMM_DIAP2.
ExpressionAtlasiO70566. baseline and differential.
GenevestigatoriO70566.

Interactioni

Subunit structurei

Interacts with MAPRE1 and APC.1 Publication

Protein-protein interaction databases

MINTiMINT-5023280.

Structurei

3D structure databases

ProteinModelPortaliO70566.
SMRiO70566. Positions 627-1067.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 463374GBD/FH3PROSITE-ProRule annotationAdd
BLAST
Domaini544 – 62077FH1Add
BLAST
Domaini625 – 1025401FH2PROSITE-ProRule annotationAdd
BLAST
Domaini1048 – 107831DADPROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili375 – 41642Sequence AnalysisAdd
BLAST
Coiled coili490 – 53950Sequence AnalysisAdd
BLAST
Coiled coili999 – 105052Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi1035 – 10384Poly-Lys
Compositional biasi1069 – 10724Arg/Lys-rich (basic)

Domaini

The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).By similarity

Sequence similaritiesi

Contains 1 DAD (diaphanous autoregulatory) domain.PROSITE-ProRule annotation
Contains 1 FH2 (formin homology 2) domain.PROSITE-ProRule annotation
Contains 1 GBD/FH3 (Rho GTPase-binding/formin homology 3) domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Phylogenomic databases

eggNOGiNOG149898.
GeneTreeiENSGT00760000118986.
HOGENOMiHOG000293231.
HOVERGENiHBG051357.
InParanoidiO70566.
KOiK05741.
PhylomeDBiO70566.

Family and domain databases

InterProiIPR016024. ARM-type_fold.
IPR027644. DIAPH2.
IPR014767. Diaphanous_autoregulatory.
IPR010465. Drf_DAD.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
[Graphical view]
PANTHERiPTHR23213:SF174. PTHR23213:SF174. 1 hit.
PfamiPF06345. Drf_DAD. 1 hit.
PF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view]
SMARTiSM00498. FH2. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70566-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MEELGAAASG AGGGGGGGEE HGGGRSNKRG AGNRAANEEE TRNKPKLRDR
60 70 80 90 100
ITSFRKSATK REKPVIQHSI DYQTAVVEIP PALIVHDDRS LILSEKEVLD
110 120 130 140 150
LFEKMMEDMN LNEEKKAPLR KKDFSIKREM VVQYISATSK SIVGSKVLGG
160 170 180 190 200
LKNSKHEFTL SSQEYVHELR SGISDEKLLN CLESLRVSLT SHPVSWVNNF
210 220 230 240 250
GYEGLGVLLD VLEKLLDKKQ QENIDKKNQY KVIQCLKAFM NNKFGLQRIL
260 270 280 290 300
GDERSLLLLA RAIDPKQQNM MTEIVKILSA ICIVGEENIL DKLLGGITAA
310 320 330 340 350
AELNNRERFS PIVEGLENNE ALHLQVACMQ FINALVTSPY DLDFRIHLRN
360 370 380 390 400
EFLRCGLKAM LPTLKEIENE GLDIQLRVFE ENKEDDLSEL SHRLNDIRAE
410 420 430 440 450
MDDINEVYHL LYNMLKDTAA EPYLLSILQH FLLIRNDYYI RPQYYKIIEE
460 470 480 490 500
CVSQIVLHCS GMDPDFKYRQ RIDFDFTHLL DACVNKAKVE ENEQKAMEFS
510 520 530 540 550
KKFDEEFTAR QEAQAELQKR DEKIKELETE IQQLRGQGVP SAIPGPPPPP
560 570 580 590 600
PLPGAGPCPP PPPPPPPPPP LPGVVPPPPP PLPGMPGIPP PPPPPLSGVP
610 620 630 640 650
PPPPPPGGVF PLLSGPIELP YGMKQKKLYK PDIPMKRINW SKIEPKELSE
660 670 680 690 700
NCVWLKLKEE KYENADLFAK LALTFPSQMK GQRNTEAAEE NRSGPPKKKV
710 720 730 740 750
KELRILDTKT AQNLSIFLGS YRMPYEEIKN IILEVNEEML SEALIQNLVK
760 770 780 790 800
YLPDQNALRE LAQLKSEYDD LCEPEQFGVV MSTVKMLRPR LTSILFKLTF
810 820 830 840 850
EEHVNNIKPS IIAVTLACEE LKKSESFKRL LELILLVGNY MNSGSRNAQS
860 870 880 890 900
LGFKINFLCK IKDTKSADQK STLLHFLAEI CDEKYRDILK FPDELEHVES
910 920 930 940 950
AGKVSAQILK SNLVAMEQSI LHLEKNIKNF PPAESHHDKF VEKMMSFTQN
960 970 980 990 1000
AREQYDKLST MHSNMLKLYE SLGEYFIFDP NTVNMEEFFG DLNTFRTLFL
1010 1020 1030 1040 1050
EALKENHKRK EMEEKSRRAK LAKEKAEQEK LERQKKKKQL IDINKEGDET
1060 1070 1080 1090
GVMDNLLEAL QSGAAFRDRR KRIPRNPDNR RPPLERSRSR HNGAMSSK
Length:1,098
Mass (Da):124,871
Last modified:February 16, 2004 - v2
Checksum:iBB19D49E049EA8CA
GO

Sequence cautioni

The sequence BAC40476.1 differs from that shown. Reason: Probable cloning artifact.

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti750 – 7512KY → RD in CAA75871. (PubMed:9497258)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK088648 mRNA. Translation: BAC40476.1. Sequence problems.
Y15910 mRNA. Translation: CAA75871.1.
RefSeqiXP_006528645.1. XM_006528582.1.
UniGeneiMm.10763.

Genome annotation databases

EnsembliENSMUST00000113320; ENSMUSP00000108946; ENSMUSG00000034480.
GeneIDi54004.
KEGGimmu:54004.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AK088648 mRNA. Translation: BAC40476.1 . Sequence problems.
Y15910 mRNA. Translation: CAA75871.1 .
RefSeqi XP_006528645.1. XM_006528582.1.
UniGenei Mm.10763.

3D structure databases

ProteinModelPortali O70566.
SMRi O70566. Positions 627-1067.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-5023280.

PTM databases

PhosphoSitei O70566.

Proteomic databases

MaxQBi O70566.
PaxDbi O70566.
PRIDEi O70566.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000113320 ; ENSMUSP00000108946 ; ENSMUSG00000034480 .
GeneIDi 54004.
KEGGi mmu:54004.

Organism-specific databases

CTDi 54004.
MGIi MGI:1858500. Diap2.

Phylogenomic databases

eggNOGi NOG149898.
GeneTreei ENSGT00760000118986.
HOGENOMi HOG000293231.
HOVERGENi HBG051357.
InParanoidi O70566.
KOi K05741.
PhylomeDBi O70566.

Miscellaneous databases

NextBioi 13841926.
PROi O70566.
SOURCEi Search...

Gene expression databases

Bgeei O70566.
CleanExi MM_DIAP2.
ExpressionAtlasi O70566. baseline and differential.
Genevestigatori O70566.

Family and domain databases

InterProi IPR016024. ARM-type_fold.
IPR027644. DIAPH2.
IPR014767. Diaphanous_autoregulatory.
IPR010465. Drf_DAD.
IPR015425. FH2_Formin.
IPR010472. FH3_dom.
IPR010473. GTPase-bd.
IPR014768. GTPase-bd/formin_homology_3.
[Graphical view ]
PANTHERi PTHR23213:SF174. PTHR23213:SF174. 1 hit.
Pfami PF06345. Drf_DAD. 1 hit.
PF06367. Drf_FH3. 1 hit.
PF06371. Drf_GBD. 1 hit.
PF02181. FH2. 1 hit.
[Graphical view ]
SMARTi SM00498. FH2. 1 hit.
[Graphical view ]
SUPFAMi SSF48371. SSF48371. 1 hit.
PROSITEi PS51231. DAD. 1 hit.
PS51444. FH2. 1 hit.
PS51232. GBD_FH3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: NOD.
    Tissue: Thymus.
  2. "A human homologue of the Drosophila melanogaster diaphanous gene is disrupted in a patient with premature ovarian failure: evidence for conserved function in oogenesis and implications for human sterility."
    Bione S., Sala C., Manzini C., Arrigo G., Zuffardi O., Banfi S., Borsani G., Jonveaux P., Philippe C., Zuccotti M., Ballabio A., Toniolo D.
    Am. J. Hum. Genet. 62:533-541(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 750-1098.
    Strain: BALB/c.
  3. "EB1 and APC bind to mDia to stabilize microtubules downstream of Rho and promote cell migration."
    Wen Y., Eng C.H., Schmoranzer J., Cabrera-Poch N., Morris E.J.S., Chen M., Wallar B.J., Alberts A.S., Gundersen G.G.
    Nat. Cell Biol. 6:820-830(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH APC AND MAPRE1.

Entry informationi

Entry nameiDIAP2_MOUSE
AccessioniPrimary (citable) accession number: O70566
Secondary accession number(s): Q8C2G8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: February 16, 2004
Last modified: October 29, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3