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O70551

- SRPK1_MOUSE

UniProt

O70551 - SRPK1_MOUSE

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Protein

SRSF protein kinase 1

Gene

Srpk1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/arginine-rich protein-specific kinase which specifically phosphorylates its substrates at serine residues located in regions rich in arginine/serine dipeptides, known as RS domains and is involved in the phosphorylation of SR splicing factors and the regulation of splicing. Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Can influence additional steps of mRNA maturation, as well as other cellular activities, such as chromatin reorganization in somatic and sperm cells and cell cycle progression. Phosphorylates SFRS2, ZRSR2, LBR and PRM1. Phosphorylates SRSF1 using a directional (C-terminal to N-terminal) and a dual-track mechanism incorporating both processive phosphorylation (in which the kinase stays attached to the substrate after each round of phosphorylation) and distributive phosphorylation steps (in which the kinase and substrate dissociate after each phosphorylation event). The RS domain of SRSF1 binds first to a docking groove in the large lobe of the kinase domain of SRPK1. This induces certain structural changes in SRPK1 and/or RRM2 domain of SRSF1, allowing RRM2 to bind the kinase and initiate phosphorylation. The cycles continue for several phosphorylation steps in a processive manner (steps 1-8) until the last few phosphorylation steps (approximately steps 9-12). During that time, a mechanical stress induces the unfolding of the beta-4 motif in RRM2, which then docks at the docking groove of SRPK1. This also signals RRM2 to begin to dissociate, which facilitates SRSF1 dissociation after phosphorylation is completed. Can mediate hepatitis B virus (HBV) core protein phosphorylation. It plays a negative role in the regulation of HBV replication through a mechanism not involving the phosphorylation of the core protein but by reducing the packaging efficiency of the pregenomic RNA (pgRNA) without affecting the formation of the viral core particles. Can induce splicing of exon 10 in MAPT/TAU (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.2 Publications

Cofactori

Mg2+2 Publications

Enzyme regulationi

Activated by phosphorylation on Ser-51 and Ser-548.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei109 – 1091ATPBy similarityPROSITE-ProRule annotation
Active sitei213 – 2131Proton acceptorBy similarityPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi86 – 949ATPBy similarityPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB
  2. magnesium ion binding Source: UniProtKB
  3. poly(A) RNA binding Source: Ensembl
  4. protein serine/threonine kinase activity Source: UniProtKB

GO - Biological processi

  1. cell differentiation Source: UniProtKB-KW
  2. chromosome segregation Source: UniProtKB
  3. intracellular signal transduction Source: UniProtKB
  4. mRNA processing Source: UniProtKB-KW
  5. negative regulation of viral genome replication Source: Ensembl
  6. positive regulation of viral genome replication Source: Ensembl
  7. protein phosphorylation Source: UniProtKB
  8. regulation of mRNA processing Source: UniProtKB
  9. RNA splicing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Chromosome partition, Differentiation, mRNA processing, mRNA splicing

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
SRSF protein kinase 1 (EC:2.7.11.1)
Alternative name(s):
SFRS protein kinase 1
Serine/arginine-rich protein-specific kinase 1
Short name:
SR-protein-specific kinase 1
Gene namesi
Name:Srpk1Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 17

Organism-specific databases

MGIiMGI:106908. Srpk1.

Subcellular locationi

Cytoplasm By similarity. Nucleus By similarity. Nucleus matrix By similarity. Microsome By similarity
Note: Shuttles between the nucleus and the cytoplasm. Inhibition of the Hsp90 ATPase activity, osmotic stress and interaction with HHV-1 ICP27 protein can induce its translocation to the nucleus. KAT5/TIP60 inhibits its nuclear translocation (By similarity).By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. endoplasmic reticulum Source: UniProtKB-KW
  3. nucleus Source: UniProtKB
  4. plasma membrane Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Microsome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 648648SRSF protein kinase 1PRO_0000086675Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei51 – 511Phosphoserine1 Publication
Modified residuei309 – 3091Phosphoserine1 Publication
Modified residuei311 – 3111Phosphoserine1 Publication
Modified residuei448 – 4481Phosphothreonine1 Publication
Modified residuei450 – 4501Phosphoserine1 Publication
Modified residuei548 – 5481Phosphoserine; by CK2By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiO70551.
PaxDbiO70551.
PRIDEiO70551.

PTM databases

PhosphoSiteiO70551.

Expressioni

Tissue specificityi

Predominantly expressed in the testis but is also present at lower levels in heart, spleen, liver, brain, kidney, lung and skeletal muscle. Present in all germinal cells in the seminiferous tubules but not in mature spermatozoa.2 Publications

Gene expression databases

BgeeiO70551.
CleanExiMM_SRPK1.
ExpressionAtlasiO70551. baseline and differential.
GenevestigatoriO70551.

Interactioni

Subunit structurei

Monomer. Found in a multisubunit complex containing seven proteins, named toposome, which separates entangled circular chromatin DNA during chromosome segregation. Interacts with HHV-1 ICP27 protein. Interacts with DNAJB1/HSP40 and AHSA1/AHA1 and this mediates formation of a complex with the Hsp70 /Hsp90 machinery. Binds to IGF2BP1, SYNCRIP, HNRNPA2B1 and HNRNPC. Interacts with SAFB/SAFB1 and SAFB2 which inhibits its activity (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
SRSF1Q079555EBI-593343,EBI-398920From a different organism.

Protein-protein interaction databases

IntActiO70551. 2 interactions.
MINTiMINT-4123549.

Structurei

3D structure databases

ProteinModelPortaliO70551.
SMRiO70551. Positions 63-263, 467-648.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 646567Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000171558.
HOVERGENiHBG108512.
InParanoidiO70551.
KOiK15409.
OMAiQSFSEQH.
OrthoDBiEOG70S74X.
PhylomeDBiO70551.
TreeFamiTF105334.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 2 hits.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 2 hits.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70551-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MERKVLALQA RKKRTKAKKD KAQRKPETQH RGSAPHSESD IPEQEEEILG
60 70 80 90 100
SDDDEQEDPN DYCKGGYHLV KIGDLFNGRY HVIRKLGWGH FSTVWLSWDI
110 120 130 140 150
QGKKFVAMKV VKSAEHYTET ALDEIRLLKS VRNSDPNDPN GEMVVQLLDD
160 170 180 190 200
FKISGVNGTH ICMVFEVLGH HLLKWIIKSN YQGLPLPCVK KIIQQVLQGL
210 220 230 240 250
DYLHTKCRII HTDIKPENIL LSVNEQYIRR LAAEATEWQR SGAPPPSGSA
260 270 280 290 300
VSTAPQPKPA DKMSKNKKKK LKKKQKRQAE LLEKRMQEIE EMEKESGPGQ
310 320 330 340 350
KRPNKQEESE SPVDRPLTEN PPNKMTQEKL EESNSIGQDQ TLTERGGEGG
360 370 380 390 400
APEINCNGVI GVVNYPENSN NETLRHKEDL HNANDCDVHT LKQEPSFLNS
410 420 430 440 450
SNGDSSPSQD TDSCTPTASE TMVCQSSAEQ SLTRQDITQL EESIRADTPS
460 470 480 490 500
GDEQEPNGAL DSKGKFSAGN FLINPLEPKN AEKLQVKIAD LGNACWVHKH
510 520 530 540 550
FTEDIQTRQY RSLEVLIGSG YNTPADIWST ACMAFELATG DYLFEPHSGE
560 570 580 590 600
DYTRDEDHIA LIIELLGKVP RKLIVAGKYS KEFFTKKGDL KHITKLKPWG
610 620 630 640
LLEVLVEKYE WPQEEAAGFT DFLLPMLELM PEKRATAAEC LRHPWLNS
Length:648
Mass (Da):73,088
Last modified:June 21, 2005 - v2
Checksum:iCB779C163A11ECEB
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131S → I in CAA11833. (PubMed:10390541)Curated
Sequence conflicti155 – 1551G → V in CAA11833. (PubMed:10390541)Curated
Sequence conflicti256 – 2561Q → T in CAA11833. (PubMed:10390541)Curated
Sequence conflicti279 – 2791A → P in BAA25299. (PubMed:9446799)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012290 mRNA. Translation: BAA25299.1.
AJ224115 mRNA. Translation: CAA11833.1.
BC005707 mRNA. Translation: AAH05707.1.
BC050761 mRNA. Translation: AAH50761.1.
CCDSiCCDS37531.1.
PIRiJC5930.
RefSeqiNP_058075.2. NM_016795.3.
UniGeneiMm.15252.
Mm.479188.

Genome annotation databases

EnsembliENSMUST00000130643; ENSMUSP00000116259; ENSMUSG00000004865.
GeneIDi20815.
KEGGimmu:20815.
UCSCiuc008bri.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB012290 mRNA. Translation: BAA25299.1 .
AJ224115 mRNA. Translation: CAA11833.1 .
BC005707 mRNA. Translation: AAH05707.1 .
BC050761 mRNA. Translation: AAH50761.1 .
CCDSi CCDS37531.1.
PIRi JC5930.
RefSeqi NP_058075.2. NM_016795.3.
UniGenei Mm.15252.
Mm.479188.

3D structure databases

ProteinModelPortali O70551.
SMRi O70551. Positions 63-263, 467-648.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O70551. 2 interactions.
MINTi MINT-4123549.

PTM databases

PhosphoSitei O70551.

Proteomic databases

MaxQBi O70551.
PaxDbi O70551.
PRIDEi O70551.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000130643 ; ENSMUSP00000116259 ; ENSMUSG00000004865 .
GeneIDi 20815.
KEGGi mmu:20815.
UCSCi uc008bri.1. mouse.

Organism-specific databases

CTDi 6732.
MGIi MGI:106908. Srpk1.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000171558.
HOVERGENi HBG108512.
InParanoidi O70551.
KOi K15409.
OMAi QSFSEQH.
OrthoDBi EOG70S74X.
PhylomeDBi O70551.
TreeFami TF105334.

Miscellaneous databases

NextBioi 299541.
PROi O70551.
SOURCEi Search...

Gene expression databases

Bgeei O70551.
CleanExi MM_SRPK1.
ExpressionAtlasi O70551. baseline and differential.
Genevestigatori O70551.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 2 hits.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 2 hits.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles."
    Kuroyanagi N., Onogi H., Wakabayashi T., Hagiwara M.
    Biochem. Biophys. Res. Commun. 242:357-364(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Brain1 Publication.
  2. "SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1."
    Papoutsopoulou S., Nikolakaki E., Chalepakis G., Kruft V., Chevaillier P., Giannakouros T.
    Nucleic Acids Res. 27:2972-2980(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: EmbryoImported.
  3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/NImported.
    Tissue: Mammary glandImported and TestisImported.
  4. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 573-581, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic brain.
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-51; SER-309; SER-311; THR-448 AND SER-450, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.

Entry informationi

Entry nameiSRPK1_MOUSE
AccessioniPrimary (citable) accession number: O70551
Secondary accession number(s): O70193, Q99JT3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 21, 2005
Last sequence update: June 21, 2005
Last modified: November 26, 2014
This is version 128 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3