Serine/threonine-protein kinase SRPK1

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Reviewed, UniProtKB/Swiss-Prot O70551 (SRPK1_MOUSE)

Last modified June 16, 2009. Version 79. History...

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Serine/threonine-protein kinase SRPK1
    EC=2.7.11.1
Alternative name(s):
    Serine/arginine-rich protein-specific kinase 1
      Short name=SR-protein-specific kinase 1
    SFRS protein kinase 1

Gene names

Name:

Srpk1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

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Protein attributes

Sequence length	648 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

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General annotation (Comments)

Function	Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Hyperphosphorylates RS domain-containing proteins such as SFRS1 and SFRS2 on serine residues during metaphase but at lower levels during interphase. Locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. Ref.1 Ref.2 UniProtKB Q96SB4
Catalytic activity	ATP + a protein = ADP + a phosphoprotein. Ref.1 Ref.2
Cofactor	Magnesium. Ref.1 Ref.2
Enzyme regulation	Activated by phosphorylation on Ser-51 and Ser-548 By similarity. UniProtKB Q96SB4
Subunit structure	Present in a seven component complex, the toposome, which separates entangled circular chromatin DNA during chromosome segregation By similarity. UniProtKB Q96SB4
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Predominantly expressed in the testis but is also present at lower levels in heart, spleen, liver, brain, kidney, lung and skeletal muscle. Present in all germinal cells in the seminiferous tubules but not in mature spermatozoa. Ref.1 Ref.2
Sequence similarities	Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. Contains 1 protein kinase domain.

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Ontologies

Keywords
Biological process	Chromosome partition Differentiation mRNA processing mRNA splicing
Cellular component	Cytoplasm Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	RNA splicing Inferred from electronic annotation. Source: UniProtKB-KW cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW chromosome segregation Inferred from sequence or structural similarity. Source: UniProtKB mRNA processing Inferred from electronic annotation. Source: UniProtKB-KW protein amino acid phosphorylation Ref.1 Inferred from direct assay. Source: UniProtKB protein kinase cascade Ref.1 Inferred from direct assay. Source: UniProtKB regulation of mRNA processing Ref.1 Inferred from direct assay. Source: UniProtKB
Cellular component	cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Ref.1 Inferred from direct assay. Source: UniProtKB magnesium ion binding Ref.1 Inferred from direct assay. Source: UniProtKB protein binding Inferred from physical interaction. Source: IntAct protein serine/threonine kinase activity Ref.1 Inferred from direct assay. Source: UniProtKB
Complete GO annotation...

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Binary interactions

With	Entry	#Exp.	IntAct	Notes
SFRS1	Q07955	4	EBI-593343,EBI-398920	From a different organism.

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 648

648

Serine/threonine-protein kinase SRPK1

PRO_0000086675

Regions

Domain

80 – 646

567

Protein kinase

Nucleotide binding

86 – 94

ATP By similarity UniProtKB Q9UPE1

Sites

Active site

213

Proton acceptor By similarity UniProtKB Q9UPE1

Binding site

109

ATP By similarity UniProtKB Q9UPE1

Amino acid modifications

Modified residue

Phosphoserine; by CK2 By similarity UniProtKB Q96SB4

Modified residue

309

Phosphoserine Ref.7

Modified residue

311

Phosphoserine Ref.7

Modified residue

448

Phosphothreonine Ref.7 Ref.5 Ref.6

Modified residue

450

Phosphoserine Ref.7 Ref.5 Ref.6

Modified residue

548

Phosphoserine; by CK2 By similarity UniProtKB Q96SB4

Experimental info

Sequence conflict

113

S → I in CAA11833. Ref.2

Sequence conflict

155

G → V in CAA11833. Ref.2

Sequence conflict

256

Q → T in CAA11833. Ref.2

Sequence conflict

279

A → P in BAA25299. Ref.1

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Sequences

Sequence

Length

Mass (Da)

Tools

O70551-1 [UniParc].

Last modified June 21, 2005. Version 2.
Checksum: CB779C163A11ECEB
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FASTA

648

73,088

        10         20         30         40         50         60 
MERKVLALQA RKKRTKAKKD KAQRKPETQH RGSAPHSESD IPEQEEEILG SDDDEQEDPN 

        70         80         90        100        110        120 
DYCKGGYHLV KIGDLFNGRY HVIRKLGWGH FSTVWLSWDI QGKKFVAMKV VKSAEHYTET 

       130        140        150        160        170        180 
ALDEIRLLKS VRNSDPNDPN GEMVVQLLDD FKISGVNGTH ICMVFEVLGH HLLKWIIKSN 

       190        200        210        220        230        240 
YQGLPLPCVK KIIQQVLQGL DYLHTKCRII HTDIKPENIL LSVNEQYIRR LAAEATEWQR 

       250        260        270        280        290        300 
SGAPPPSGSA VSTAPQPKPA DKMSKNKKKK LKKKQKRQAE LLEKRMQEIE EMEKESGPGQ 

       310        320        330        340        350        360 
KRPNKQEESE SPVDRPLTEN PPNKMTQEKL EESNSIGQDQ TLTERGGEGG APEINCNGVI 

       370        380        390        400        410        420 
GVVNYPENSN NETLRHKEDL HNANDCDVHT LKQEPSFLNS SNGDSSPSQD TDSCTPTASE 

       430        440        450        460        470        480 
TMVCQSSAEQ SLTRQDITQL EESIRADTPS GDEQEPNGAL DSKGKFSAGN FLINPLEPKN 

       490        500        510        520        530        540 
AEKLQVKIAD LGNACWVHKH FTEDIQTRQY RSLEVLIGSG YNTPADIWST ACMAFELATG 

       550        560        570        580        590        600 
DYLFEPHSGE DYTRDEDHIA LIIELLGKVP RKLIVAGKYS KEFFTKKGDL KHITKLKPWG 

       610        620        630        640 
LLEVLVEKYE WPQEEAAGFT DFLLPMLELM PEKRATAAEC LRHPWLNS

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Novel SR-protein-specific kinase, SRPK2, disassembles nuclear speckles." Kuroyanagi N., Onogi H., Wakabayashi T., Hagiwara M. Biochem. Biophys. Res. Commun. 242:357-364(1998) [PubMed: 9446799] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Brain.
[2]	"SR protein-specific kinase 1 is highly expressed in testis and phosphorylates protamine 1." Papoutsopoulou S., Nikolakaki E., Chalepakis G., Kruft V., Chevaillier P., Giannakouros T. Nucleic Acids Res. 27:2972-2980(1999) [PubMed: 10390541] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY. Tissue: Embryo.
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland and Testis.
[4]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 573-581, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[5]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-448 AND SER-450, MASS SPECTROMETRY. Tissue: Brain.
[6]	"Protein phosphorylation and expression profiling by Yin-yang multidimensional liquid chromatography (Yin-yang MDLC) mass spectrometry." Dai J., Jin W.-H., Sheng Q.-H., Shieh C.-H., Wu J.-R., Zeng R. J. Proteome Res. 6:250-262(2007) [PubMed: 17203969] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-448 AND SER-450, MASS SPECTROMETRY. Tissue: Liver.
[7]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-309; SER-311; THR-448 AND SER-450, MASS SPECTROMETRY. Tissue: Liver.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	AB012290 mRNA. Translation: BAA25299.1. AJ224115 mRNA. Translation: CAA11833.1. BC005707 mRNA. Translation: AAH05707.1. BC050761 mRNA. Translation: AAH50761.1.
IPI	IPI00387234.
PIR	JC5930.
RefSeq	NP_058075.2.
UniGene	Mm.15252 Mm.462657
3D structure databases
HSSP	HSSP built from PDB template 1HOW based on UniProtKB Q03656.
SMR	O70551. Positions 65-263, 467-648.
ModBase	Search...
Protein-protein interaction databases
IntAct	O70551. 1 interaction.
PTM databases
PhosphoSite	O70551.
Proteomic databases
PRIDE	O70551.
Genome annotation databases
Ensembl	ENSMUSG00000004865. Mus musculus. [Contig view]
GeneID	20815.
KEGG	mmu:20815.
Organism-specific databases
MGI	MGI:106908. Srpk1.
Phylogenomic databases
HOGENOM	O70551.
HOVERGEN	O70551.
OMA	O70551. EQHISQL.
Enzyme and pathway databases
BRENDA	2.7.11.1. 244.
Gene expression databases
ArrayExpress	O70551.
Bgee	O70551.
CleanEx	MM_SRPK1.
GermOnline	ENSMUSG00000004865. Mus musculus.
Family and domain databases
InterPro	IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. [Graphical view]
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
ProDom	PD000001. Prot_kinase. 3 hits. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	299541.
SOURCE	Search...

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Entry information

Entry name

SRPK1_MOUSE

Accession

Primary (citable) accession number: O70551
Secondary accession number(s): O70193, Q99JT3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 21, 2005
Last sequence update:	June 21, 2005
Last modified:	June 16, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other Resources

Entry information

Relevant documents