Reviewed, UniProtKB/Swiss-Prot O70536 (SOAT1_RAT)

Names and origin

Protein names

Recommended name:
    Sterol O-acyltransferase 1
    EC=2.3.1.26
Alternative name(s):
    Cholesterol acyltransferase 1
    Acyl coenzyme A:cholesterol acyltransferase 1
      Short name=ACAT-1

Gene names

Name:	Soat1
Synonyms:	Acact, Acat

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	545 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the formation of fatty acid-cholesterol esters. Plays a role in lipoprotein assembly and dietary cholesterol absorption.
Catalytic activity	Acyl-CoA + cholesterol = CoA + cholesterol ester.
Subcellular location	Endoplasmic reticulum membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the membrane-bound acyltransferase family. Sterol o-acyltransferase subfamily.

Ontologies

Keywords
Biological process	Cholesterol metabolism Lipid metabolism Steroid metabolism
Cellular component	Endoplasmic reticulum Membrane
Domain	Transmembrane
Molecular function	Acyltransferase Transferase
Gene Ontology (GO)
Biological process	cholesterol metabolic process Ref.1 Traceable author statement. Source: RGD
Cellular component	endoplasmic reticulum Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Traceable author statement. Source: RGD
Molecular function	cholesterol O-acyltransferase activity Inferred from electronic annotation. Source: EC ergosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC lanosterol O-acyltransferase activity Inferred from electronic annotation. Source: EC phosphatidylcholine-sterol O-acyltransferase activity Inferred from expression pattern. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 545	545	Sterol O-acyltransferase 1		PRO_0000207643
Regions
	Transmembrane	136 – 156	21	Potential
	Transmembrane	316 – 336	21	Potential
	Transmembrane	357 – 377	21	Potential
	Transmembrane	465 – 485	21	Potential
	Transmembrane	493 – 513	21	Potential
Sites
	Active site	455	1	Potential

Sequences

	Sequence		Length	Mass (Da)
	O70536-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 40129EF21257BEBF	FASTA	545	64,146
	10 20 30 40 50 60 MVGEETSLRN RLSRSAENPE QDEAQKNLLD THRNGHITMK QLIAKKRQLA AEAEELKPLF 70 80 90 100 110 120 LKEVGCHFDD FVTNLIDKSA SLDNGGCALT TFSILEEMKN NHRAKDLRAP PEQGKIFISR 130 140 150 160 170 180 RSLLDELFEV DHIRTIYHMF IALLIIFILS TLVVDYIDEG RLVLEFSLLA YAFGQFPIVI 190 200 210 220 230 240 WTWWAMFLST LAIPYFLFQR WAHGYSKSSH PLIYSLIHGA FFLVFQLGIL GFIPTYVVLA 250 260 270 280 290 300 YTLPPASRFI LILEQIRLVM KAHSYVRENV PRVLSAAKEK SSTVPVPTVN QYLYFLFAPT 310 320 330 340 350 360 LIYRDSYPRT PTVRWGYVAM QFLQVFGCLF YVYYIFERLC APLFRNIKQE PFSARVLVLC 370 380 390 400 410 420 VFNSILPGVL MLFLSFFAFL HCWLNAFAEM LRFGDRMFYK DWWNSTSYSN YYRTWNVVVH 430 440 450 460 470 480 DWLYYYVYKD LLWFFSKRFR PAAMLAVFAL SAVVHEYALA VCLSYFYPVL FVLFMFFGMA 490 500 510 520 530 540 FNFIVNDSRK RPVWNIMVRA SLFLGHGVIL CFYSQEWYAR QRCPLKNPTF LDYVRPRTWT CRYVF

References

[1]	"Molecular cloning, functional expression and tissue distribution of rat acyl-coenzyme A:cholesterol acyltransferase." Matsuda H., Hakamata H., Kawasaki T., Sakashita N., Miyazaki A., Takahashi K., Shichiri M., Horiuchi S. Biochim. Biophys. Acta 1391:193-203(1998) [PubMed: 9555010] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Wistar. Tissue: Adrenal gland.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D86373 mRNA. Translation: BAA25372.1.
IPI	IPI00199499.
RefSeq	NP_112380.1.
UniGene	Rn.59
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	O70536.
Genome annotation databases
Ensembl	ENSRNOT00000005677; ENSRNOP00000005677; ENSRNOG00000004111; Rattus norvegicus. [Genome view]
GeneID	81782.
KEGG	rno:81782.
UCSC	NM_031118. rat.
Organism-specific databases
CTD	81782.
RGD	621641. Soat1.
Phylogenomic databases
HOVERGEN	O70536.
Enzyme and pathway databases
BRENDA	2.3.1.26. 248.
Gene expression databases
ArrayExpress	O70536.
Genevestigator	O70536.
GermOnline	ENSRNOG00000004111. Rattus norvegicus.
Family and domain databases
InterPro	IPR004299. MBOAT_fam. [Graphical view]
Pfam	PF03062. MBOAT. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	615631.

Entry information

Entry name

SOAT1_RAT

Accession

Primary (citable) accession number: O70536

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 18, 2001
Last sequence update:	August 1, 1998
Last modified:	October 13, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)