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O70521 (RGS19_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Regulator of G-protein signaling 19

Short name=RGS19
Alternative name(s):
G-alpha-interacting protein
Short name=GAIP
Gene names
Name:Rgs19
Synonyms:Gaip
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length216 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, predominantly to G(i)-alpha-3. Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein By similarity.

Subunit structure

Interacts with GIPC PDZ domain.

Subcellular location

Membrane; Lipid-anchor By similarity.

Post-translational modification

Fatty acylated. Heavily palmitoylated in the cysteine string motif By similarity.

Phosphorylated, mainly on serine residues. Ref.3

Sequence similarities

Contains 1 RGS domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 216216Regulator of G-protein signaling 19
PRO_0000204231

Regions

Domain90 – 206117RGS
Region207 – 21610Interaction with GIPC
Compositional bias39 – 4911Poly-Cys

Amino acid modifications

Modified residue241Phosphoserine; by CK2 Ref.3
Modified residue971Phosphoserine By similarity
Modified residue1511Phosphoserine; by MAPK1 and MAPK3 By similarity

Experimental info

Mutagenesis241S → A: 50% reduction of phosphorylation. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O70521 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 554C479D4DD89238

FASTA21624,738
        10         20         30         40         50         60 
MPTPHEAEKQ HTGPEEADRP PSMSSHDAAP SGPPSRNPCC LCWCCCCSCS WNQERQRAWQ 

        70         80         90        100        110        120 
VSRESKLQPL PSCEVCTPPS PEEVQSWAQS FDKLMHSPTG RSVFRAFLRT EYSEENMLFW 

       130        140        150        160        170        180 
LACEELKTEA DRHVVDEKAR LIYEDYVSIL SPKEVSLDSR VREGINRKMQ EPSPHTFDDA 

       190        200        210 
QLQIYTLMHR DSYPRFLTSP TYRSLLLQGA PQSSEA 

« Hide

References

« Hide 'large scale' references
[1]"RGS-GAIP, a GTPase-activating protein for Galphai heterotrimeric G proteins, is located on clathrin-coated vesicles."
de Vries L., Elenko E., McCaffery J.M., Fischer T., Hubler L., McQuistan T., Watson N., Farquhar M.G.
Mol. Biol. Cell 9:1123-1134(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Pituitary.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Spleen.
[3]"Membrane-associated GAIP is a phosphoprotein and can be phosphorylated by clathrin-coated vesicles."
Fischer T., Elenko E., Wan L., Thomas G., Farquhar M.G.
Proc. Natl. Acad. Sci. U.S.A. 97:4040-4045(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-24, MUTAGENESIS OF SER-24.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF068136 mRNA. Translation: AAC19130.1.
BC088141 mRNA. Translation: AAH88141.1.
RefSeqNP_067693.1. NM_021661.2.
UniGeneRn.88166.

3D structure databases

ProteinModelPortalO70521.
SMRO70521. Positions 79-206.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid248744. 1 interaction.
MINTMINT-191738.
STRING10116.ENSRNOP00000022451.

PTM databases

PhosphoSiteO70521.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000022451; ENSRNOP00000022451; ENSRNOG00000016547.
GeneID59293.
KEGGrno:59293.
UCSCRGD:629471. rat.

Organism-specific databases

CTD10287.
RGD629471. Rgs19.

Phylogenomic databases

eggNOGNOG258376.
GeneTreeENSGT00750000117371.
HOGENOMHOG000233513.
HOVERGENHBG013233.
InParanoidO70521.
KOK16449.
OMAFDKLMHS.
OrthoDBEOG7SN8DQ.
PhylomeDBO70521.

Gene expression databases

GenevestigatorO70521.

Family and domain databases

Gene3D1.10.196.10. 1 hit.
InterProIPR024066. Regulat_G_prot_signal_dom1.
IPR016137. Regulat_G_prot_signal_superfam.
IPR000342. RGS_dom.
[Graphical view]
PfamPF00615. RGS. 1 hit.
[Graphical view]
PRINTSPR01301. RGSPROTEIN.
SMARTSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMSSF48097. SSF48097. 1 hit.
PROSITEPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio611813.
PROO70521.

Entry information

Entry nameRGS19_RAT
AccessionPrimary (citable) accession number: O70521
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: April 16, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families