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Protein

Regulator of G-protein signaling 19

Gene

Rgs19

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, predominantly to G(i)-alpha-3. Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein (By similarity).By similarity

GO - Molecular functioni

  • G-protein alpha-subunit binding Source: RGD
  • GTPase activator activity Source: RGD

GO - Biological processi

  • positive regulation of GTPase activity Source: GOC
  • regulation of G-protein coupled receptor protein signaling pathway Source: RGD
  • termination of G-protein coupled receptor signaling pathway Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Signal transduction inhibitor

Enzyme and pathway databases

ReactomeiREACT_353304. G alpha (q) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Regulator of G-protein signaling 19
Short name:
RGS19
Alternative name(s):
G-alpha-interacting protein
Short name:
GAIP
Gene namesi
Name:Rgs19
Synonyms:Gaip
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 3

Organism-specific databases

RGDi629471. Rgs19.

Subcellular locationi

GO - Cellular componenti

  • brush border Source: RGD
  • clathrin-coated vesicle Source: RGD
  • cytoplasm Source: RGD
  • Golgi membrane Source: RGD
  • membrane Source: RGD
  • membrane raft Source: RGD
  • plasma membrane Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi24 – 241S → A: 50% reduction of phosphorylation. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 216216Regulator of G-protein signaling 19PRO_0000204231Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei24 – 241Phosphoserine; by CK21 Publication
Modified residuei97 – 971PhosphoserineBy similarity
Modified residuei151 – 1511Phosphoserine; by MAPK1 and MAPK3By similarity

Post-translational modificationi

Fatty acylated. Heavily palmitoylated in the cysteine string motif (By similarity).By similarity
Phosphorylated, mainly on serine residues.1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

PTM databases

PhosphoSiteiO70521.

Expressioni

Gene expression databases

GenevisibleiO70521. RN.

Interactioni

Subunit structurei

Interacts with GIPC PDZ domain.

Protein-protein interaction databases

BioGridi248744. 1 interaction.
MINTiMINT-191738.
STRINGi10116.ENSRNOP00000022451.

Structurei

3D structure databases

ProteinModelPortaliO70521.
SMRiO70521. Positions 79-206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini90 – 206117RGSPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni207 – 21610Interaction with GIPC

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi39 – 4911Poly-CysAdd
BLAST

Sequence similaritiesi

Contains 1 RGS domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiNOG258376.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233513.
HOVERGENiHBG013233.
InParanoidiO70521.
KOiK16449.
OMAiLMHSPAG.
OrthoDBiEOG7SN8DQ.
PhylomeDBiO70521.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70521-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPTPHEAEKQ HTGPEEADRP PSMSSHDAAP SGPPSRNPCC LCWCCCCSCS
60 70 80 90 100
WNQERQRAWQ VSRESKLQPL PSCEVCTPPS PEEVQSWAQS FDKLMHSPTG
110 120 130 140 150
RSVFRAFLRT EYSEENMLFW LACEELKTEA DRHVVDEKAR LIYEDYVSIL
160 170 180 190 200
SPKEVSLDSR VREGINRKMQ EPSPHTFDDA QLQIYTLMHR DSYPRFLTSP
210
TYRSLLLQGA PQSSEA
Length:216
Mass (Da):24,738
Last modified:August 1, 1998 - v1
Checksum:i554C479D4DD89238
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068136 mRNA. Translation: AAC19130.1.
BC088141 mRNA. Translation: AAH88141.1.
RefSeqiNP_067693.1. NM_021661.2.
UniGeneiRn.88166.

Genome annotation databases

EnsembliENSRNOT00000022451; ENSRNOP00000022451; ENSRNOG00000016547.
GeneIDi59293.
KEGGirno:59293.
UCSCiRGD:629471. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF068136 mRNA. Translation: AAC19130.1.
BC088141 mRNA. Translation: AAH88141.1.
RefSeqiNP_067693.1. NM_021661.2.
UniGeneiRn.88166.

3D structure databases

ProteinModelPortaliO70521.
SMRiO70521. Positions 79-206.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248744. 1 interaction.
MINTiMINT-191738.
STRINGi10116.ENSRNOP00000022451.

PTM databases

PhosphoSiteiO70521.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000022451; ENSRNOP00000022451; ENSRNOG00000016547.
GeneIDi59293.
KEGGirno:59293.
UCSCiRGD:629471. rat.

Organism-specific databases

CTDi10287.
RGDi629471. Rgs19.

Phylogenomic databases

eggNOGiNOG258376.
GeneTreeiENSGT00760000118903.
HOGENOMiHOG000233513.
HOVERGENiHBG013233.
InParanoidiO70521.
KOiK16449.
OMAiLMHSPAG.
OrthoDBiEOG7SN8DQ.
PhylomeDBiO70521.

Enzyme and pathway databases

ReactomeiREACT_353304. G alpha (q) signalling events.

Miscellaneous databases

NextBioi611813.
PROiO70521.

Gene expression databases

GenevisibleiO70521. RN.

Family and domain databases

Gene3Di1.10.196.10. 1 hit.
InterProiIPR016137. RGS.
IPR024066. RGS_subdom1.
[Graphical view]
PfamiPF00615. RGS. 1 hit.
[Graphical view]
PRINTSiPR01301. RGSPROTEIN.
SMARTiSM00315. RGS. 1 hit.
[Graphical view]
SUPFAMiSSF48097. SSF48097. 1 hit.
PROSITEiPS50132. RGS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "RGS-GAIP, a GTPase-activating protein for Galphai heterotrimeric G proteins, is located on clathrin-coated vesicles."
    de Vries L., Elenko E., McCaffery J.M., Fischer T., Hubler L., McQuistan T., Watson N., Farquhar M.G.
    Mol. Biol. Cell 9:1123-1134(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pituitary.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Spleen.
  3. "Membrane-associated GAIP is a phosphoprotein and can be phosphorylated by clathrin-coated vesicles."
    Fischer T., Elenko E., Wan L., Thomas G., Farquhar M.G.
    Proc. Natl. Acad. Sci. U.S.A. 97:4040-4045(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION AT SER-24, MUTAGENESIS OF SER-24.

Entry informationi

Entry nameiRGS19_RAT
AccessioniPrimary (citable) accession number: O70521
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: June 24, 2015
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.