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Protein

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

Gene

Hcn4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) that regulate the rhythm of heart beat. May contribute to the native pacemaker currents in neurons (Ih) (By similarity). May mediate responses to sour stimuli.By similarity1 Publication

Enzyme regulationi

Activated by cAMP. cAMP binding causes a conformation change that leads to the assembly of an active tetramer and channel opening (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi659 – 662cAMPBy similarity4
Nucleotide bindingi669 – 670cAMPBy similarity2

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Potassium channel, Sodium channel, Voltage-gated channel
Biological processIon transport, Potassium transport, Sodium transport, Transport
LigandcAMP, cAMP-binding, Nucleotide-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4
Alternative name(s):
Brain cyclic nucleotide-gated channel 3
Short name:
BCNG-3
Gene namesi
Name:Hcn4
Synonyms:Bcng3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1298209 Hcn4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 266CytoplasmicSequence analysisAdd BLAST266
Transmembranei267 – 287Helical; Name=Segment S1Sequence analysisAdd BLAST21
Topological domaini288 – 293ExtracellularSequence analysis6
Transmembranei294 – 314Helical; Name=Segment S2Sequence analysisAdd BLAST21
Topological domaini315 – 340CytoplasmicSequence analysisAdd BLAST26
Transmembranei341 – 361Helical; Name=Segment S3Sequence analysisAdd BLAST21
Topological domaini362 – 368ExtracellularSequence analysis7
Transmembranei369 – 389Helical; Voltage-sensor; Name=Segment S4Sequence analysisAdd BLAST21
Topological domaini390 – 420CytoplasmicSequence analysisAdd BLAST31
Transmembranei421 – 441Helical; Name=Segment S5Sequence analysisAdd BLAST21
Topological domaini442 – 464ExtracellularSequence analysisAdd BLAST23
Intramembranei465 – 486Pore-forming; Name=Segment H5Sequence analysisAdd BLAST22
Topological domaini487 – 496ExtracellularSequence analysis10
Transmembranei497 – 517Helical; Name=Segment S6Sequence analysisAdd BLAST21
Topological domaini518 – 1186CytoplasmicSequence analysisAdd BLAST669

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

GuidetoPHARMACOLOGYi403

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000541181 – 1186Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4Add BLAST1186

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139PhosphoserineBy similarity1
Glycosylationi458N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1090PhosphoserineCombined sources1
Modified residuei1093PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiO70507
PaxDbiO70507
PeptideAtlasiO70507
PRIDEiO70507

PTM databases

iPTMnetiO70507
PhosphoSitePlusiO70507
SwissPalmiO70507

Expressioni

Tissue specificityi

Detected in a subset of elongated cells in taste buds.1 Publication

Interactioni

Subunit structurei

Homotetramer. The potassium channel is composed of a homo- or heterotetrameric complex of pore-forming subunits (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiO70507, 2 interactors
STRINGi10090.ENSMUSP00000034889

Structurei

3D structure databases

ProteinModelPortaliO70507
SMRiO70507
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni209 – 260Involved in subunit assemblyBy similarityAdd BLAST52

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi784 – 1061Pro/Ser-richAdd BLAST278

Domaini

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Sequence similaritiesi

Belongs to the potassium channel HCN family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0498 Eukaryota
ENOG410XPSE LUCA
HOVERGENiHBG039490
InParanoidiO70507
PhylomeDBiO70507

Family and domain databases

CDDicd00038 CAP_ED, 1 hit
Gene3Di2.60.120.10, 1 hit
InterProiView protein in InterPro
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR030173 HCN4
IPR005821 Ion_trans_dom
IPR013621 Ion_trans_N
IPR003938 K_chnl_volt-dep_EAG/ELK/ERG
IPR014710 RmlC-like_jellyroll
PANTHERiPTHR10217:SF375 PTHR10217:SF375, 1 hit
PfamiView protein in Pfam
PF00027 cNMP_binding, 1 hit
PF00520 Ion_trans, 1 hit
PF08412 Ion_trans_N, 1 hit
PRINTSiPR01463 EAGCHANLFMLY
SMARTiView protein in SMART
SM00100 cNMP, 1 hit
SUPFAMiSSF51206 SSF51206, 1 hit
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 1 hit
PS50042 CNMP_BINDING_3, 1 hit

Sequencei

Sequence statusi: Complete.

O70507-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDKLPPSMRK RLYSLPQQVG AKAWIMDEEE DGEEEGAGGR QDPSRRSIRL
60 70 80 90 100
RPLPSPSPSV AAGCSESRGA ALGATESEGP GRSAGKSSTN GDCRRFRGSL
110 120 130 140 150
ASLGSRGGGS GGAGGGSSLG HLHDSAEERR LIAAEGDASP GEDRTPPGLA
160 170 180 190 200
TEPERPATAA QPAASPPPQQ PPQPASASCE QPSADTAIKV EGGAAAIDHI
210 220 230 240 250
LPEAEVRLGQ SGFMQRQFGA MLQPGVNKFS LRMFGSQKAV EREQERVKSA
260 270 280 290 300
GFWIIHPYSD FRFYWDLTML LLMVGNLIII PVGITFFKDE NTTPWIVFNV
310 320 330 340 350
VSDTFFLIDL VLNFRTGIVV EDNTEIILDP QRIKMKYLKS WFVVDFISSI
360 370 380 390 400
PVEYIFLIVE TRIDSEVYKT ARAVRIVRFT KILSLLRLLR LSRLIRYIHQ
410 420 430 440 450
WEEIFHMTYD LASAVVRIVN LIGMMLLLCH WDGCLQFLVP MLQDFPHDCW
460 470 480 490 500
VSINGMVNNS WGKQYSYALF KAMSHMLCIG YGRQAPVGMS DVWLTMLSMI
510 520 530 540 550
VGATCYAMFI GHATALIQSL DSSRRQYQEK YKQVEQYMSF HKLPPDTRQR
560 570 580 590 600
IHDYYEHRYQ GKMFDEESIL GELSEPLREE IINFNCRKLV ASMPLFANAD
610 620 630 640 650
PNFVTSMLTK LRFEVFQPGD YIIREGTIGK KMYFIQHGVV SVLTKGNKET
660 670 680 690 700
RLADGSYFGE ICLLTRGRRT ASVRADTYCR LYSLSVDNFN EVLEEYPMMR
710 720 730 740 750
KKNSILLHKV QHDLNSGVFN YQENEIIQQI VRHDREMAHC AHRVQAAASA
760 770 780 790 800
TPTPTPVIWT PLIQAPLQAA AATTSVAIAL THHPRLPAAI FRPPPGPGLG
810 820 830 840 850
NLGAGQTPRH PRRLQSLIPS ALGSASPASS PSQVDTPSSS SFHIQQLAGF
860 870 880 890 900
SAPPGLSPLL PSSSSSPPPG ACGSPPAPTP STSTAAAAST TGFGHFHKAL
910 920 930 940 950
GGSLSSSDSP LLTPLQPGAR SPQAAQPPPP LPGARGGLGL LEHFLPPPPS
960 970 980 990 1000
SRSPSSSPGQ LGQPPGELSL GLAAGPSSTP ETPPRPERPS FMAGASGGAS
1010 1020 1030 1040 1050
PVAFTPRGGL SPPGHSPGPP RTFPSAPPRA SGSHGSLLLP PASSPPPPQV
1060 1070 1080 1090 1100
PQRRGTPPLT PGRLTQDLKL ISASQPALPQ DGAQTLRRAS PHSSGESVAA
1110 1120 1130 1140 1150
FSLYPRAGGG SGSSGGLGPP GRPYGAIPGQ HVTLPRKTSS GSLPPPLSLF
1160 1170 1180
GARAASSGGP PLTTAAPQRE PGARSEPVRS KLPSNL
Length:1,186
Mass (Da):127,414
Last modified:February 28, 2003 - v2
Checksum:iB3DC16818E8697DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC110530 Genomic DNA No translation available.
AF064874 mRNA Translation: AAC40126.1
UniGeneiMm.151967

Similar proteinsi

Entry informationi

Entry nameiHCN4_MOUSE
AccessioniPrimary (citable) accession number: O70507
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 28, 2003
Last sequence update: February 28, 2003
Last modified: March 28, 2018
This is version 138 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health