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O70496 (CLCN7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
H(+)/Cl(-) exchange transporter 7
Alternative name(s):
Chloride channel 7 alpha subunit
Chloride channel protein 7
Short name=ClC-7
Gene names
Name:Clcn7
Synonyms:Clc7
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length803 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Slowly voltage-gated channel mediating the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the lysosome lumen. Ref.3 Ref.4

Subunit structure

Chloride channel 7 are heteromers of alpha (CLCN7) and beta (OSTM1) subunits. Ref.3

Subcellular location

Lysosome membrane; Multi-pass membrane protein Ref.3.

Disruption phenotype

Accumulates undegraded endocyted material in neurons or renal proximal tubular cells. Almost all ClC-7-deficient neurons die. Ref.4

Miscellaneous

The CLC channel family contains both chloride channels and proton-coupled anion transporters that exchange chloride or another anion for protons. The presence of conserved gating glutamate residues is typical for family members that function as antiporters By similarity.

Sequence similarities

Belongs to the chloride channel (TC 2.A.49) family. ClC-7/CLCN7 subfamily. [View classification]

Contains 2 CBS domains.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Ostm1Q8BGT07EBI-987482,EBI-987431

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 803803H(+)/Cl(-) exchange transporter 7
PRO_0000094453

Regions

Topological domain1 – 124124Cytoplasmic By similarity
Transmembrane125 – 15733Helical; By similarity
Transmembrane172 – 19524Helical; By similarity
Intramembrane204 – 2118Helical; By similarity
Transmembrane221 – 23919Helical; By similarity
Transmembrane245 – 26218Helical; By similarity
Intramembrane286 – 29813Helical; By similarity
Intramembrane302 – 3109Helical; By similarity
Transmembrane320 – 33920Helical; By similarity
Transmembrane373 – 40331Helical; By similarity
Transmembrane408 – 43023Helical; By similarity
Transmembrane485 – 50521Helical; By similarity
Transmembrane510 – 53324Helical; By similarity
Intramembrane543 – 55715Helical; By similarity
Intramembrane558 – 5603Note=Loop between two helices; By similarity
Intramembrane561 – 57212Helical; By similarity
Intramembrane573 – 5764Note=Loop between two helices; By similarity
Transmembrane577 – 59519Helical; By similarity
Topological domain596 – 803208Cytoplasmic By similarity
Domain629 – 69365CBS 1
Domain739 – 79759CBS 2
Nucleotide binding656 – 6583ATP By similarity
Nucleotide binding781 – 7844ATP By similarity
Motif201 – 2055Selectivity filter part_1 By similarity
Motif243 – 2475Selectivity filter part_2 By similarity
Motif510 – 5145Selectivity filter part_3 By similarity

Sites

Binding site2021Chloride By similarity
Binding site5121Chloride; via amide nitrogen By similarity
Binding site6001Chloride By similarity
Site2451Mediates proton transfer from the outer aqueous phase to the interior of the protein; involved in linking H(+) and Cl(-) transport By similarity
Site3121Mediates proton transfer from the protein to the inner aqueous phase By similarity

Amino acid modifications

Modified residue91Phosphoserine Ref.5

Sequences

Sequence LengthMass (Da)Tools
O70496 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: A7D6DA5791DAA48C

FASTA80388,713
        10         20         30         40         50         60 
MANVSKKVSW SGRDRDDEEG APLLRRTGQP DEETPLLNGA GPGARQSHSA LFRIGQMNNV 

        70         80         90        100        110        120 
ELDDELLDPE VDPPHTFPKE IPHNEKLLSL KYESLDYDNS ENQLFLEEER RINHTAFRTV 

       130        140        150        160        170        180 
EIKRWVICAL IGILTGLVAC FIDIVVENLA GLKYRVIKDN IDKFTEKGGL SFSLLLWATL 

       190        200        210        220        230        240 
NSAFVLVGSV IVAFIEPVAA GSGIPQIKCF LNGVKIPHVV RLKTLVIKVS GVILSVVGGL 

       250        260        270        280        290        300 
AVGKEGPMIH SGSVIAAGIS QGRSTSLKRD FKIFEYFRRD TEKRDFVSAG AAAGVSAAFG 

       310        320        330        340        350        360 
APVGGVLFSL EEGASFWNQF LTWRIFFASM ISTFTLNFVL SIYHGNMWDL SSPGLINFGR 

       370        380        390        400        410        420 
FDSEKMAYTI HEIPVFIAMG VVGGILGAVF NALNYWLTMF RIRYIHRPCL QVIEAMLVAA 

       430        440        450        460        470        480 
VTATVAFVLI YSSRDCQPLQ GSSMSYPLQL FCADGEYNSM AAAFFNTPEK SVVSLFHDPP 

       490        500        510        520        530        540 
GSYNPMTLGL FTLVYFFLAC WTYGLTVSAG VFIPSLLIGA AWGRLFGISL SYLTGAAIWA 

       550        560        570        580        590        600 
DPGKYALMGA AAQLGGIVRM TLSLTVIMME ATSNVTYGFP IMLVLMTAKI VGDVFIEGLY 

       610        620        630        640        650        660 
DMHIQLQSVP FLHWEAPVTS HSLTAREVMS TPVTCLRRRE KVGIIVDVLS DTASNHNGFP 

       670        680        690        700        710        720 
VVEDVGDTQP ARLQGLILRS QLIVLLKHKV FVERSNMGLV QRRLRLKDFR DAYPRFPPIQ 

       730        740        750        760        770        780 
SIHVSQDERE CTMDLSEFMN PSPYTVPQEA SLPRVFKLFR ALGLRHLVVV DNHNQVVGLV 

       790        800 
TRKDLARYRL GKGGLEELSL AQT 

« Hide

References

« Hide 'large scale' references
[1]"Loss of the ClC-7 chloride channel leads to osteopetrosis in mice and man."
Kornak U., Kasper D., Boesl M.R., Kaiser E., Schweizer M., Schulz A., Friedrich W., Delling G., Jentsch T.J.
Cell 104:205-215(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Brain.
[3]"ClC-7 requires Ostm1 as a beta-subunit to support bone resorption and lysosomal function."
Lange P.F., Wartosch L., Jentsch T.J., Fuhrmann J.C.
Nature 440:220-223(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, INTERACTION WITH OSTM1, SUBCELLULAR LOCATION.
[4]"Lysosomal degradation of endocytosed proteins depends on the chloride transport protein ClC-7."
Wartosch L., Fuhrmann J.C., Schweizer M., Stauber T., Jentsch T.J.
FASEB J. 23:4056-4068(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DISRUPTION PHENOTYPE.
[5]"The phagosomal proteome in interferon-gamma-activated macrophages."
Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-9, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF063101 expand/collapse EMBL AC list , AF063098, AF063099, AF063100 Genomic DNA. Translation: AAC18832.1.
BC050907 mRNA. Translation: AAH50907.1.
BC053049 mRNA. Translation: AAH53049.1.
BC054799 mRNA. Translation: AAH54799.1.
RefSeqNP_036060.1. NM_011930.3.
UniGeneMm.270587.

3D structure databases

ProteinModelPortalO70496.
SMRO70496. Positions 119-790.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204933. 1 interaction.
IntActO70496. 2 interactions.
MINTMINT-4090953.

PTM databases

PhosphoSiteO70496.

Proteomic databases

PaxDbO70496.
PRIDEO70496.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000040729; ENSMUSP00000035964; ENSMUSG00000036636.
GeneID26373.
KEGGmmu:26373.
UCSCuc008azv.1. mouse.

Organism-specific databases

CTD1186.
MGIMGI:1347048. Clcn7.

Phylogenomic databases

eggNOGCOG0038.
HOGENOMHOG000231081.
HOVERGENHBG050985.
InParanoidO70496.
KOK05016.
OMAHEIPIFI.
OrthoDBEOG77Q4W6.
TreeFamTF313867.

Gene expression databases

ArrayExpressO70496.
BgeeO70496.
CleanExMM_CLCN7.
GenevestigatorO70496.

Family and domain databases

Gene3D1.10.3080.10. 2 hits.
InterProIPR000644. CBS_dom.
IPR014743. Cl-channel_core.
IPR001807. Cl-channel_volt-gated.
IPR002249. Cl_channel-7.
[Graphical view]
PfamPF00571. CBS. 2 hits.
PF00654. Voltage_CLC. 1 hit.
[Graphical view]
PRINTSPR00762. CLCHANNEL.
PR01118. CLCHANNEL7.
SMARTSM00116. CBS. 2 hits.
[Graphical view]
SUPFAMSSF81340. SSF81340. 2 hits.
PROSITEPS51371. CBS. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio304273.
PROO70496.
SOURCESearch...

Entry information

Entry nameCLCN7_MOUSE
AccessionPrimary (citable) accession number: O70496
Entry history
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 1, 1998
Last modified: March 19, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot