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Protein

Sorting nexin-3

Gene

Snx3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol 3-phosphate (PtdIns(P3)). Also can bind phosphatidylinositol 4-phosphate (PtdIns(P4)), phosphatidylinositol 5-phosphate (PtdIns(P5)) and phosphatidylinositol 3,5-biphosphate (PtdIns(3,5)P2) (PubMed:19576982). Plays a role in protein transport between cellular compartments. Together with RAB7A facilitates endosome membrane association of the retromer cargo-selective subcomplex (CSC). May act in part as component of the SNX3-retromer complex which mediates the retrograde endosome-to-TGN transport of WLS distinct from the SNX-BAR retromer pathway (By similarity). Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G (PubMed:18632802). Not involved in EGFR degradation. Involved in the regulation of phagocytosis in dendritic cells possibly by regulating EEA1 recruitment to the nascent phagosomes (By similarity). Involved in iron homeostasis through regulation of endocytic recycling of the transferrin receptor Tfrc presuambly by delivering the tranferrin:transferrin receptor complex to recycling endosomes; the function may involve the CSC retromer subcomplex (PubMed:23416069). Involved in regulation of neurite outgrowth in primary neurons (PubMed:19576982).By similarity2 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei70 – 701Phosphatidylinositol 3-phosphateBy similarity
Binding sitei72 – 721Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity
Binding sitei95 – 951Phosphatidylinositol 3-phosphateBy similarity
Binding sitei118 – 1181Phosphatidylinositol 3-phosphateBy similarity

GO - Molecular functioni

  • phosphatidylinositol-3,5-bisphosphate binding Source: UniProtKB
  • phosphatidylinositol-3-phosphate binding Source: UniProtKB
  • phosphatidylinositol-4-phosphate binding Source: UniProtKB
  • phosphatidylinositol-5-phosphate binding Source: UniProtKB
  • protein phosphatase binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-3
Alternative name(s):
SDP3 protein
Gene namesi
Name:Snx3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1860188. Snx3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi152 – 1521K → A: Abolishes phosphoinositide-binding of C-terminus; when associated with A-158, A-160 and A-161. 1 Publication
Mutagenesisi158 – 1581K → A: Abolishes phosphoinositide-binding of C-terminus; when associated with A-152, A-160 and A-161. 1 Publication
Mutagenesisi160 – 1601R → A: Abolishes phosphoinositide-binding of C-terminus; when associated with A-152, A-160 and A-161. 1 Publication
Mutagenesisi161 – 1611H → A: Abolishes phosphoinositide-binding of C-terminus; when associated with A-152, A-158 and A-160. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 162161Sorting nexin-3PRO_0000213841Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity
Modified residuei72 – 721PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP10.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO70492.
MaxQBiO70492.
PaxDbiO70492.
PeptideAtlasiO70492.
PRIDEiO70492.

2D gel databases

REPRODUCTION-2DPAGEO70492.

PTM databases

iPTMnetiO70492.
PhosphoSiteiO70492.

Expressioni

Tissue specificityi

Highly expressed in developing red cells and hematopoietic tissues (PubMed:23416069).1 Publication

Gene expression databases

CleanExiMM_SNX3.

Interactioni

Subunit structurei

Interacts with VPS26A, VPS29 (By similarity). Interacts with VPS35; the interaction with VPS35 is direct (PubMed:23416069). The association with the retromer CSC subcomplex subunits is proposed to represent a functional distinct retromer variant described as SNX3-retromer complex (By similarity). Interacts with USP10 and SCNN1A (PubMed:18632802). Interacts with TRFC (PubMed:23416069). Interacts with SNX8; 2 molecules of SNX8 seems to associate with one molecule of SNX3. Interacts with PTPRU (By similarity).By similarity2 Publications

GO - Molecular functioni

Protein-protein interaction databases

IntActiO70492. 2 interactions.
MINTiMINT-4135125.
STRINGi10090.ENSMUSP00000019939.

Structurei

3D structure databases

ProteinModelPortaliO70492.
SMRiO70492. Positions 24-148.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini27 – 151125PXPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni147 – 16216Binds predominantly to PtdIns(P5) and weaker to PtdIns(P3) abd PtdIns(P4); involved in neurite outgrowth regulation1 PublicationAdd
BLAST

Domaini

The PX domain mediates specific binding to phosphatidylinositol 3-phosphate (PtdIns(P3)).

Sequence similaritiesi

Belongs to the sorting nexin family.Curated
Contains 1 PX (phox homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2527. Eukaryota.
COG5391. LUCA.
HOGENOMiHOG000234882.
HOVERGENiHBG055338.
InParanoidiO70492.
PhylomeDBiO70492.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
[Graphical view]
PfamiPF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70492-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAETVADTRR LITKPQNLND AYGPPSNFLE IDVSNPQTVG VGRGRFTTYE
60 70 80 90 100
IRVKTNLPIF KLKESTVRRR YSDFEWLRSE LERESKVVVP PLPGKAFLRH
110 120 130 140 150
VPFRGDDGIF DDNFIEERKQ GLEQFINKVA GHPLAQNERC LHMFLQDEII
160
DKSYTPSKIR HA
Length:162
Mass (Da):18,757
Last modified:January 23, 2007 - v3
Checksum:i8DDF851FCA7404A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062482 mRNA. Translation: AAC16017.1.
UniGeneiMm.277870.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062482 mRNA. Translation: AAC16017.1.
UniGeneiMm.277870.

3D structure databases

ProteinModelPortaliO70492.
SMRiO70492. Positions 24-148.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70492. 2 interactions.
MINTiMINT-4135125.
STRINGi10090.ENSMUSP00000019939.

PTM databases

iPTMnetiO70492.
PhosphoSiteiO70492.

2D gel databases

REPRODUCTION-2DPAGEO70492.

Proteomic databases

EPDiO70492.
MaxQBiO70492.
PaxDbiO70492.
PeptideAtlasiO70492.
PRIDEiO70492.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1860188. Snx3.

Phylogenomic databases

eggNOGiKOG2527. Eukaryota.
COG5391. LUCA.
HOGENOMiHOG000234882.
HOVERGENiHBG055338.
InParanoidiO70492.
PhylomeDBiO70492.

Miscellaneous databases

ChiTaRSiSnx3. mouse.
PROiO70492.
SOURCEiSearch...

Gene expression databases

CleanExiMM_SNX3.

Family and domain databases

Gene3Di3.30.1520.10. 1 hit.
InterProiIPR001683. Phox.
[Graphical view]
PfamiPF00787. PX. 1 hit.
[Graphical view]
SMARTiSM00312. PX. 1 hit.
[Graphical view]
SUPFAMiSSF64268. SSF64268. 1 hit.
PROSITEiPS50195. PX. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiSNX3_MOUSE
AccessioniPrimary (citable) accession number: O70492
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: July 6, 2016
This is version 102 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.