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Protein

Acyl-coenzyme A synthetase ACSM2, mitochondrial

Gene

Acsm2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro) (By similarity).By similarity

Catalytic activityi

ATP + a carboxylate + CoA = AMP + diphosphate + an acyl-CoA.

Cofactori

Mg2+By similarity, Mn2+By similarity

Enzyme regulationi

Activated by monovalent cations, such as potassium, rubidium or ammonium.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei139 – 1391Coenzyme ABy similarity
Binding sitei364 – 3641SubstrateBy similarity
Binding sitei446 – 4461ATPBy similarity
Binding sitei461 – 4611ATPBy similarity
Binding sitei472 – 4721SubstrateBy similarity
Binding sitei501 – 5011Coenzyme ABy similarity
Binding sitei532 – 5321Coenzyme ABy similarity
Binding sitei557 – 5571ATPBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi221 – 2299ATPBy similarity
Nucleotide bindingi359 – 3646ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. butyrate-CoA ligase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. fatty acid metabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Fatty acid metabolism, Lipid metabolism

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_275205. Conjugation of salicylate with glycine.
REACT_288339. Conjugation of benzoate with glycine.
REACT_349279. Conjugation of phenylacetate with glutamine.

Names & Taxonomyi

Protein namesi
Recommended name:
Acyl-coenzyme A synthetase ACSM2, mitochondrial (EC:6.2.1.2)
Alternative name(s):
Acyl-CoA synthetase medium-chain family member 2
Butyrate--CoA ligase 2
Butyryl-coenzyme A synthetase 2
Kidney-specific protein KS
Middle-chain acyl-CoA synthetase 2
Gene namesi
Name:Acsm2
Synonyms:Ks
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi708383. Acsm2.

Subcellular locationi

Mitochondrion matrix By similarity

GO - Cellular componenti

  1. mitochondrial matrix Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4646MitochondrionSequence AnalysisAdd
BLAST
Chaini47 – 572526Acyl-coenzyme A synthetase ACSM2, mitochondrialPRO_0000306096Add
BLAST

Proteomic databases

PaxDbiO70490.
PRIDEiO70490.

PTM databases

PhosphoSiteiO70490.

Expressioni

Tissue specificityi

Detected in kidney, in proximal tubules.1 Publication

Developmental stagei

First detected in kidney from 1 week old rats. Not detectable in fetal kidney and in kidney from newborn rats.1 Publication

Inductioni

Down-regulated in kidneys from a strain of spontaneously hypertensive rats (SHR). Down-regulated after unilateral ureteral obstruction or unilateral nephrectomy.1 Publication

Gene expression databases

GenevestigatoriO70490.

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020587.

Structurei

3D structure databases

ProteinModelPortaliO70490.
SMRiO70490. Positions 34-569.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni469 – 4713Coenzyme A bindingBy similarity
Regioni540 – 5423Coenzyme A bindingBy similarity

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229982.
HOVERGENiHBG053031.
InParanoidiO70490.
KOiK01896.
OrthoDBiEOG7D85VZ.
PhylomeDBiO70490.
TreeFamiTF354287.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70490-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHWLWKIPRL CTFWGTEMFH RTFHMNIKKL MPIQWGHQEV PAKFNFASDV
60 70 80 90 100
IDHWASLEKA GKRSPGPALW WMNGSGEELK WNFRELSEIS KQTANVLTGA
110 120 130 140 150
CGLQRGDRVA VVLPRVPEWW LVTLGCMRSG LVFMPGTTQM KSTDILYRLQ
160 170 180 190 200
SSKARAIVAG DEVVQEVDAV APDCSFLKIK LLVSEKNREG WLNFKALLKD
210 220 230 240 250
ASPIHQCVET VSQESAAIYF TSGTSGPPKM AEHSHCSLGL KAKMDAGWTG
260 270 280 290 300
LGPSDTMWTI SDTGWILNIL GSFLEPWVLG TCIFVHLLPK FDPQTVLKVL
310 320 330 340 350
SSYPINTLLG APLIYRMLLQ QDLSSYKFPH LHSCFSGGET LLPETLESWK
360 370 380 390 400
AKTGLEIREI YGQTETGITC RVSRTMKVKP GYLGTAIVPY DVQVIDEQGN
410 420 430 440 450
VLPPGKEGDM ALRVKPIRPI GMFSGYVDNP KKTQANIRGD FWLLGDRGIK
460 470 480 490 500
DTEGYFHFMG RTDDIINSSG YRIGPSEVEN ALMEHPAVVE TAVISSPDPI
510 520 530 540 550
RREVVKAFVV LAPEFLSHDQ DQLTKVLQEH VKSVTAPYKY PRKVEFVLDL
560 570
PKTITGKIER AKLRAKEWKT SG
Length:572
Mass (Da):64,145
Last modified:October 2, 2007 - v2
Checksum:i34BB5FB87A43958C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti22 – 221T → S in AAD05209 (PubMed:9844120).Curated
Sequence conflicti99 – 991G → R in AAH78721 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062389 mRNA. Translation: AAD05209.1.
BC078721 mRNA. Translation: AAH78721.1.
RefSeqiNP_653349.1. NM_144748.1.
XP_008757899.1. XM_008759677.1.
UniGeneiRn.14875.

Genome annotation databases

GeneIDi246263.
KEGGirno:246263.
UCSCiRGD:708383. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF062389 mRNA. Translation: AAD05209.1.
BC078721 mRNA. Translation: AAH78721.1.
RefSeqiNP_653349.1. NM_144748.1.
XP_008757899.1. XM_008759677.1.
UniGeneiRn.14875.

3D structure databases

ProteinModelPortaliO70490.
SMRiO70490. Positions 34-569.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000020587.

PTM databases

PhosphoSiteiO70490.

Proteomic databases

PaxDbiO70490.
PRIDEiO70490.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi246263.
KEGGirno:246263.
UCSCiRGD:708383. rat.

Organism-specific databases

CTDi123876.
RGDi708383. Acsm2.

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229982.
HOVERGENiHBG053031.
InParanoidiO70490.
KOiK01896.
OrthoDBiEOG7D85VZ.
PhylomeDBiO70490.
TreeFamiTF354287.

Enzyme and pathway databases

ReactomeiREACT_275205. Conjugation of salicylate with glycine.
REACT_288339. Conjugation of benzoate with glycine.
REACT_349279. Conjugation of phenylacetate with glutamine.

Miscellaneous databases

NextBioi623619.

Gene expression databases

GenevestigatoriO70490.

Family and domain databases

InterProiIPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning of KS, a novel rat gene expressed exclusively in the kidney."
    Hilgers K.F., Nagaraj S.K., Karginova E.A., Kazakova I.G., Chevalier R.L., Carey R.M., Pentz E.S., Gomez R.A.
    Kidney Int. 54:1444-1454(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, INDUCTION, TISSUE SPECIFICITY.
    Strain: Sprague-Dawley.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Kidney.

Entry informationi

Entry nameiACSM2_RAT
AccessioniPrimary (citable) accession number: O70490
Secondary accession number(s): Q6AZ63
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 2, 2007
Last sequence update: October 2, 2007
Last modified: April 1, 2015
This is version 82 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.