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O70490

- ACSM2_RAT

UniProt

O70490 - ACSM2_RAT

Protein

Acyl-coenzyme A synthetase ACSM2, mitochondrial

Gene

Acsm2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 2 (02 Oct 2007)
      Previous versions | rss
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    Functioni

    Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C4 to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro) By similarity.By similarity

    Catalytic activityi

    ATP + a carboxylate + CoA = AMP + diphosphate + an acyl-CoA.

    Cofactori

    Magnesium or manganese.By similarity

    Enzyme regulationi

    Activated by monovalent cations, such as potassium, rubidium or ammonium.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei139 – 1391Coenzyme ABy similarity
    Binding sitei364 – 3641SubstrateBy similarity
    Binding sitei446 – 4461ATPBy similarity
    Binding sitei461 – 4611ATPBy similarity
    Binding sitei472 – 4721SubstrateBy similarity
    Binding sitei501 – 5011Coenzyme ABy similarity
    Binding sitei532 – 5321Coenzyme ABy similarity
    Binding sitei557 – 5571ATPBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi221 – 2299ATPBy similarity
    Nucleotide bindingi359 – 3646ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. butyrate-CoA ligase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. fatty acid metabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Fatty acid metabolism, Lipid metabolism

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A synthetase ACSM2, mitochondrial (EC:6.2.1.2)
    Alternative name(s):
    Acyl-CoA synthetase medium-chain family member 2
    Butyrate--CoA ligase 2
    Butyryl-coenzyme A synthetase 2
    Kidney-specific protein KS
    Middle-chain acyl-CoA synthetase 2
    Gene namesi
    Name:Acsm2
    Synonyms:Ks
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi708383. Acsm2.

    Subcellular locationi

    Mitochondrion matrix By similarity

    GO - Cellular componenti

    1. mitochondrial matrix Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Mitochondrion

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4646MitochondrionSequence AnalysisAdd
    BLAST
    Chaini47 – 572526Acyl-coenzyme A synthetase ACSM2, mitochondrialPRO_0000306096Add
    BLAST

    Proteomic databases

    PaxDbiO70490.
    PRIDEiO70490.

    PTM databases

    PhosphoSiteiO70490.

    Expressioni

    Tissue specificityi

    Detected in kidney, in proximal tubules.1 Publication

    Developmental stagei

    First detected in kidney from 1 week old rats. Not detectable in fetal kidney and in kidney from newborn rats.1 Publication

    Inductioni

    Down-regulated in kidneys from a strain of spontaneously hypertensive rats (SHR). Down-regulated after unilateral ureteral obstruction or unilateral nephrectomy.1 Publication

    Gene expression databases

    GenevestigatoriO70490.

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000020587.

    Structurei

    3D structure databases

    ProteinModelPortaliO70490.
    SMRiO70490. Positions 34-569.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni469 – 4713Coenzyme A bindingBy similarity
    Regioni540 – 5423Coenzyme A bindingBy similarity

    Sequence similaritiesi

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0365.
    GeneTreeiENSGT00750000117290.
    HOGENOMiHOG000229982.
    HOVERGENiHBG053031.
    InParanoidiO70490.
    KOiK01896.
    OrthoDBiEOG7D85VZ.
    PhylomeDBiO70490.
    TreeFamiTF354287.

    Family and domain databases

    InterProiIPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view]
    PfamiPF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view]
    PROSITEiPS00455. AMP_BINDING. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O70490-1 [UniParc]FASTAAdd to Basket

    « Hide

    MHWLWKIPRL CTFWGTEMFH RTFHMNIKKL MPIQWGHQEV PAKFNFASDV    50
    IDHWASLEKA GKRSPGPALW WMNGSGEELK WNFRELSEIS KQTANVLTGA 100
    CGLQRGDRVA VVLPRVPEWW LVTLGCMRSG LVFMPGTTQM KSTDILYRLQ 150
    SSKARAIVAG DEVVQEVDAV APDCSFLKIK LLVSEKNREG WLNFKALLKD 200
    ASPIHQCVET VSQESAAIYF TSGTSGPPKM AEHSHCSLGL KAKMDAGWTG 250
    LGPSDTMWTI SDTGWILNIL GSFLEPWVLG TCIFVHLLPK FDPQTVLKVL 300
    SSYPINTLLG APLIYRMLLQ QDLSSYKFPH LHSCFSGGET LLPETLESWK 350
    AKTGLEIREI YGQTETGITC RVSRTMKVKP GYLGTAIVPY DVQVIDEQGN 400
    VLPPGKEGDM ALRVKPIRPI GMFSGYVDNP KKTQANIRGD FWLLGDRGIK 450
    DTEGYFHFMG RTDDIINSSG YRIGPSEVEN ALMEHPAVVE TAVISSPDPI 500
    RREVVKAFVV LAPEFLSHDQ DQLTKVLQEH VKSVTAPYKY PRKVEFVLDL 550
    PKTITGKIER AKLRAKEWKT SG 572
    Length:572
    Mass (Da):64,145
    Last modified:October 2, 2007 - v2
    Checksum:i34BB5FB87A43958C
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti22 – 221T → S in AAD05209. (PubMed:9844120)Curated
    Sequence conflicti99 – 991G → R in AAH78721. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF062389 mRNA. Translation: AAD05209.1.
    BC078721 mRNA. Translation: AAH78721.1.
    RefSeqiNP_653349.1. NM_144748.1.
    UniGeneiRn.14875.

    Genome annotation databases

    EnsembliENSRNOT00000020587; ENSRNOP00000020587; ENSRNOG00000014976.
    GeneIDi246263.
    KEGGirno:246263.
    UCSCiRGD:708383. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF062389 mRNA. Translation: AAD05209.1 .
    BC078721 mRNA. Translation: AAH78721.1 .
    RefSeqi NP_653349.1. NM_144748.1.
    UniGenei Rn.14875.

    3D structure databases

    ProteinModelPortali O70490.
    SMRi O70490. Positions 34-569.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000020587.

    PTM databases

    PhosphoSitei O70490.

    Proteomic databases

    PaxDbi O70490.
    PRIDEi O70490.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000020587 ; ENSRNOP00000020587 ; ENSRNOG00000014976 .
    GeneIDi 246263.
    KEGGi rno:246263.
    UCSCi RGD:708383. rat.

    Organism-specific databases

    CTDi 123876.
    RGDi 708383. Acsm2.

    Phylogenomic databases

    eggNOGi COG0365.
    GeneTreei ENSGT00750000117290.
    HOGENOMi HOG000229982.
    HOVERGENi HBG053031.
    InParanoidi O70490.
    KOi K01896.
    OrthoDBi EOG7D85VZ.
    PhylomeDBi O70490.
    TreeFami TF354287.

    Miscellaneous databases

    NextBioi 623619.

    Gene expression databases

    Genevestigatori O70490.

    Family and domain databases

    InterProi IPR025110. AMP-bd_C.
    IPR020845. AMP-binding_CS.
    IPR000873. AMP-dep_Synth/Lig.
    [Graphical view ]
    Pfami PF00501. AMP-binding. 1 hit.
    PF13193. AMP-binding_C. 1 hit.
    [Graphical view ]
    PROSITEi PS00455. AMP_BINDING. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of KS, a novel rat gene expressed exclusively in the kidney."
      Hilgers K.F., Nagaraj S.K., Karginova E.A., Kazakova I.G., Chevalier R.L., Carey R.M., Pentz E.S., Gomez R.A.
      Kidney Int. 54:1444-1454(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], DEVELOPMENTAL STAGE, INDUCTION, TISSUE SPECIFICITY.
      Strain: Sprague-Dawley.
      Tissue: Kidney.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.

    Entry informationi

    Entry nameiACSM2_RAT
    AccessioniPrimary (citable) accession number: O70490
    Secondary accession number(s): Q6AZ63
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 2, 2007
    Last sequence update: October 2, 2007
    Last modified: October 1, 2014
    This is version 77 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3