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Protein

Vesicle-associated membrane protein 4

Gene

Vamp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • exocytosis Source: GO_Central
  • Golgi ribbon formation Source: MGI
  • Golgi to plasma membrane protein transport Source: MGI
  • microtubule cytoskeleton organization Source: MGI
  • regulation of Golgi to plasma membrane protein transport Source: MGI
  • SNARE complex assembly Source: MGI
  • toxin transport Source: MGI
  • vesicle fusion Source: GO_Central
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 4
Short name:
VAMP-4
Gene namesi
Name:Vamp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1858730. Vamp4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 118118CytoplasmicSequence analysisAdd
BLAST
Transmembranei119 – 13921Helical; Anchor for type IV membrane proteinSequence analysisAdd
BLAST
Topological domaini140 – 1412VesicularSequence analysis

GO - Cellular componenti

  • cell surface Source: MGI
  • Golgi apparatus Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • SNARE complex Source: MGI
  • trans-Golgi network Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 141141Vesicle-associated membrane protein 4PRO_0000206732Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei17 – 171PhosphoserineCombined sources
Modified residuei30 – 301PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO70480.
MaxQBiO70480.
PaxDbiO70480.
PeptideAtlasiO70480.
PRIDEiO70480.

PTM databases

iPTMnetiO70480.
PhosphoSiteiO70480.
SwissPalmiO70480.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026696.
CleanExiMM_VAMP4.
ExpressionAtlasiO70480. baseline and differential.
GenevisibleiO70480. MM.

Interactioni

Subunit structurei

Identified in a complex containing STX6, STX12, VAMP4 and VTI1A.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiO70480. 3 interactions.
MINTiMINT-4139753.
STRINGi10090.ENSMUSP00000051544.

Structurei

Secondary structure

1
141
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi51 – 533Combined sources
Helixi54 – 574Combined sources
Helixi60 – 10950Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2NPSX-ray2.50A47-117[»]
ProteinModelPortaliO70480.
SMRiO70480. Positions 49-111.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO70480.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini52 – 11261v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated
Contains 1 v-SNARE coiled-coil homology domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG061695.
InParanoidiO70480.
OMAiWRGCKMK.
OrthoDBiEOG091G0MPE.
PhylomeDBiO70480.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN
60 70 80 90 100
DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN
110 120 130 140
RSKQLRRQMW WRGCKIKAIM ALAAAILLLM IIILIVVKFR T
Length:141
Mass (Da):16,353
Last modified:August 1, 1998 - v1
Checksum:i556CCCB8213CF91E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti113 – 1131G → V in BAB27774 (PubMed:16141072).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061516 mRNA. Translation: AAC15776.1.
AK011678 mRNA. Translation: BAB27774.1.
AK018344 mRNA. Translation: BAB31171.1.
RefSeqiXP_006496997.1. XM_006496934.1.
XP_006496998.1. XM_006496935.1.
UniGeneiMm.10699.

Genome annotation databases

EnsembliENSMUST00000135241; ENSMUSP00000116376; ENSMUSG00000026696.
ENSMUST00000150040; ENSMUSP00000115133; ENSMUSG00000026696.
ENSMUST00000155003; ENSMUSP00000114172; ENSMUSG00000026696.
GeneIDi53330.
UCSCiuc007dgk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061516 mRNA. Translation: AAC15776.1.
AK011678 mRNA. Translation: BAB27774.1.
AK018344 mRNA. Translation: BAB31171.1.
RefSeqiXP_006496997.1. XM_006496934.1.
XP_006496998.1. XM_006496935.1.
UniGeneiMm.10699.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2NPSX-ray2.50A47-117[»]
ProteinModelPortaliO70480.
SMRiO70480. Positions 49-111.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70480. 3 interactions.
MINTiMINT-4139753.
STRINGi10090.ENSMUSP00000051544.

PTM databases

iPTMnetiO70480.
PhosphoSiteiO70480.
SwissPalmiO70480.

Proteomic databases

EPDiO70480.
MaxQBiO70480.
PaxDbiO70480.
PeptideAtlasiO70480.
PRIDEiO70480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000135241; ENSMUSP00000116376; ENSMUSG00000026696.
ENSMUST00000150040; ENSMUSP00000115133; ENSMUSG00000026696.
ENSMUST00000155003; ENSMUSP00000114172; ENSMUSG00000026696.
GeneIDi53330.
UCSCiuc007dgk.1. mouse.

Organism-specific databases

CTDi8674.
MGIiMGI:1858730. Vamp4.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG061695.
InParanoidiO70480.
OMAiWRGCKMK.
OrthoDBiEOG091G0MPE.
PhylomeDBiO70480.

Enzyme and pathway databases

ReactomeiR-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.

Miscellaneous databases

EvolutionaryTraceiO70480.
PROiO70480.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026696.
CleanExiMM_VAMP4.
ExpressionAtlasiO70480. baseline and differential.
GenevisibleiO70480. MM.

Family and domain databases

Gene3Di1.10.3840.10. 1 hit.
InterProiIPR001388. Synaptobrevin.
[Graphical view]
PfamiPF00957. Synaptobrevin. 1 hit.
[Graphical view]
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiPS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiVAMP4_MOUSE
AccessioniPrimary (citable) accession number: O70480
Secondary accession number(s): Q9D095
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.