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Protein

Vesicle-associated membrane protein 4

Gene

Vamp4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • exocytosis Source: GO_Central
  • Golgi ribbon formation Source: MGI
  • Golgi to plasma membrane protein transport Source: MGI
  • microtubule cytoskeleton organization Source: MGI
  • regulation of Golgi to plasma membrane protein transport Source: MGI
  • SNARE complex assembly Source: MGI
  • toxin transport Source: MGI
  • vesicle fusion Source: GO_Central

Enzyme and pathway databases

ReactomeiR-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Vesicle-associated membrane protein 4
Short name:
VAMP-4
Gene namesi
Name:Vamp4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1858730. Vamp4.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 118CytoplasmicSequence analysisAdd BLAST118
Transmembranei119 – 139Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST21
Topological domaini140 – 141VesicularSequence analysis2

GO - Cellular componenti

  • cell surface Source: MGI
  • Golgi apparatus Source: MGI
  • integral component of membrane Source: UniProtKB-KW
  • SNARE complex Source: MGI
  • trans-Golgi network Source: MGI

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002067321 – 141Vesicle-associated membrane protein 4Add BLAST141

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei17PhosphoserineCombined sources1
Modified residuei30PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO70480.
MaxQBiO70480.
PaxDbiO70480.
PeptideAtlasiO70480.
PRIDEiO70480.

PTM databases

iPTMnetiO70480.
PhosphoSitePlusiO70480.
SwissPalmiO70480.

Expressioni

Gene expression databases

BgeeiENSMUSG00000026696.
CleanExiMM_VAMP4.
ExpressionAtlasiO70480. baseline and differential.
GenevisibleiO70480. MM.

Interactioni

Subunit structurei

Identified in a complex containing STX6, STX12, VAMP4 and VTI1A.1 Publication

GO - Molecular functioni

Protein-protein interaction databases

IntActiO70480. 3 interactors.
MINTiMINT-4139753.
STRINGi10090.ENSMUSP00000051544.

Structurei

Secondary structure

1141
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi51 – 53Combined sources3
Helixi54 – 57Combined sources4
Helixi60 – 109Combined sources50

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NPSX-ray2.50A47-117[»]
ProteinModelPortaliO70480.
SMRiO70480.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO70480.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini52 – 112v-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST61

Sequence similaritiesi

Belongs to the synaptobrevin family.Curated

Keywords - Domaini

Coiled coil, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG061695.
InParanoidiO70480.
OMAiWRGCKMK.
OrthoDBiEOG091G0MPE.
PhylomeDBiO70480.

Family and domain databases

InterProiView protein in InterPro
IPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
PfamiView protein in Pfam
PF00957. Synaptobrevin. 1 hit.
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiView protein in PROSITE
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.

Sequencei

Sequence statusi: Complete.

O70480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN
60 70 80 90 100
DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN
110 120 130 140
RSKQLRRQMW WRGCKIKAIM ALAAAILLLM IIILIVVKFR T
Length:141
Mass (Da):16,353
Last modified:August 1, 1998 - v1
Checksum:i556CCCB8213CF91E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113G → V in BAB27774 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061516 mRNA. Translation: AAC15776.1.
AK011678 mRNA. Translation: BAB27774.1.
AK018344 mRNA. Translation: BAB31171.1.
CCDSiCCDS83616.1.
RefSeqiNP_001334054.1. NM_001347125.1.
XP_006496997.1. XM_006496934.2.
UniGeneiMm.10699.

Genome annotation databases

EnsembliENSMUST00000135241; ENSMUSP00000116376; ENSMUSG00000026696.
ENSMUST00000150040; ENSMUSP00000115133; ENSMUSG00000026696.
ENSMUST00000155003; ENSMUSP00000114172; ENSMUSG00000026696.
GeneIDi53330.
UCSCiuc007dgk.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061516 mRNA. Translation: AAC15776.1.
AK011678 mRNA. Translation: BAB27774.1.
AK018344 mRNA. Translation: BAB31171.1.
CCDSiCCDS83616.1.
RefSeqiNP_001334054.1. NM_001347125.1.
XP_006496997.1. XM_006496934.2.
UniGeneiMm.10699.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2NPSX-ray2.50A47-117[»]
ProteinModelPortaliO70480.
SMRiO70480.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70480. 3 interactors.
MINTiMINT-4139753.
STRINGi10090.ENSMUSP00000051544.

PTM databases

iPTMnetiO70480.
PhosphoSitePlusiO70480.
SwissPalmiO70480.

Proteomic databases

EPDiO70480.
MaxQBiO70480.
PaxDbiO70480.
PeptideAtlasiO70480.
PRIDEiO70480.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000135241; ENSMUSP00000116376; ENSMUSG00000026696.
ENSMUST00000150040; ENSMUSP00000115133; ENSMUSG00000026696.
ENSMUST00000155003; ENSMUSP00000114172; ENSMUSG00000026696.
GeneIDi53330.
UCSCiuc007dgk.1. mouse.

Organism-specific databases

CTDi8674.
MGIiMGI:1858730. Vamp4.

Phylogenomic databases

eggNOGiKOG0860. Eukaryota.
COG5143. LUCA.
GeneTreeiENSGT00550000074449.
HOGENOMiHOG000042711.
HOVERGENiHBG061695.
InParanoidiO70480.
OMAiWRGCKMK.
OrthoDBiEOG091G0MPE.
PhylomeDBiO70480.

Enzyme and pathway databases

ReactomeiR-MMU-6811440. Retrograde transport at the Trans-Golgi-Network.
R-MMU-8856825. Cargo recognition for clathrin-mediated endocytosis.
R-MMU-8856828. Clathrin-mediated endocytosis.

Miscellaneous databases

EvolutionaryTraceiO70480.
PROiPR:O70480.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000026696.
CleanExiMM_VAMP4.
ExpressionAtlasiO70480. baseline and differential.
GenevisibleiO70480. MM.

Family and domain databases

InterProiView protein in InterPro
IPR001388. Synaptobrevin.
IPR016444. Synaptobrevin/VAMP.
PfamiView protein in Pfam
PF00957. Synaptobrevin. 1 hit.
PIRSFiPIRSF005409. Synaptobrevin_euk. 1 hit.
PRINTSiPR00219. SYNAPTOBREVN.
PROSITEiView protein in PROSITE
PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiVAMP4_MOUSE
AccessioniPrimary (citable) accession number: O70480
Secondary accession number(s): Q9D095
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 21, 2001
Last sequence update: August 1, 1998
Last modified: June 7, 2017
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.