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O70475

- UGDH_MOUSE

UniProt

O70475 - UGDH_MOUSE

Protein

UDP-glucose 6-dehydrogenase

Gene

Ugdh

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.1 Publication

    Catalytic activityi

    UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.1 Publication

    Enzyme regulationi

    UDP-alpha-D-xylose (UDX) acts as a feedback inhibitor by activating an allosteric switch.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei36 – 361NADBy similarity
    Binding sitei41 – 411NADBy similarity
    Binding sitei165 – 1651NADBy similarity
    Active sitei276 – 2761NucleophileBy similarity
    Binding sitei346 – 3461NADBy similarity
    Binding sitei442 – 4421SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi11 – 166NADBy similarity
    Nucleotide bindingi89 – 935NADBy similarity
    Nucleotide bindingi130 – 1312NADBy similarity
    Nucleotide bindingi276 – 2794NADBy similarity

    GO - Molecular functioni

    1. NAD binding Source: InterPro
    2. UDP-glucose 6-dehydrogenase activity Source: UniProtKB-EC

    GO - Biological processi

    1. gastrulation with mouth forming second Source: MGI
    2. UDP-glucuronate biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00038; UER00491.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    UDP-glucose 6-dehydrogenase (EC:1.1.1.22)
    Short name:
    UDP-Glc dehydrogenase
    Short name:
    UDP-GlcDH
    Short name:
    UDPGDH
    Gene namesi
    Name:Ugdh
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 5

    Organism-specific databases

    MGIiMGI:1306785. Ugdh.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 493493UDP-glucose 6-dehydrogenasePRO_0000074061Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei107 – 1071N6-acetyllysine1 Publication
    Modified residuei474 – 4741Phosphothreonine2 Publications

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiO70475.
    PaxDbiO70475.
    PRIDEiO70475.

    PTM databases

    PhosphoSiteiO70475.

    Expressioni

    Gene expression databases

    ArrayExpressiO70475.
    BgeeiO70475.
    CleanExiMM_UGDH.
    GenevestigatoriO70475.

    Interactioni

    Subunit structurei

    Homohexamer.By similarity

    Protein-protein interaction databases

    IntActiO70475. 3 interactions.
    MINTiMINT-1854432.

    Structurei

    3D structure databases

    ProteinModelPortaliO70475.
    SMRiO70475. Positions 1-466.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni161 – 1655Substrate bindingBy similarity
    Regioni220 – 2278Substrate bindingBy similarity
    Regioni260 – 27314Substrate bindingBy similarityAdd
    BLAST
    Regioni338 – 3392Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1004.
    HOGENOMiHOG000153773.
    HOVERGENiHBG003512.
    InParanoidiO70475.
    KOiK00012.
    OMAiRETNPTA.
    PhylomeDBiO70475.
    TreeFamiTF105671.

    Family and domain databases

    Gene3Di3.40.50.720. 2 hits.
    InterProiIPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028356. UDPglc_DH_euk.
    [Graphical view]
    PANTHERiPTHR11374. PTHR11374. 1 hit.
    PTHR11374:SF3. PTHR11374:SF3. 1 hit.
    PfamiPF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    O70475-1 [UniParc]FASTAAdd to Basket

    « Hide

    MVEIKKICCI GAGYVGGPTC SVIAHMCPEI RVTVVDVNEA RINAWNSPTL    50
    PIYEPGLKEV VESCRGKNLF FSTNIDDAIR EADLVFISVN TPTKTYGMGK 100
    GRAADLKYIE ACARRIVQNS NGYKIVTEKS TVPVRAAESI RRIFDANTKP 150
    NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI GGDETPEGQK AVRALCAVYE 200
    HWVPKEKILT TNTWSSELSK LAANAFLAQR ISSINSISAL CEATGADVEE 250
    VATAIGMDQR IGNKFLKASV GFGGSCFQKD VLNLVYLCEA LNLPEVARYW 300
    QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS 350
    IYISKYLMDE GAHLHIYDPK VPREQIVVDL SHPGVSADDQ VSRLVTISKD 400
    PYEACDGAHA LVICTEWDMF KELDYERIHK KMLKPAFIFD GRRVLDGLHS 450
    ELQTIGFQIE TIGKKVSSKR IPYTPGEIPK FSLQDPPNKK PKV 493
    Length:493
    Mass (Da):54,832
    Last modified:August 1, 1998 - v1
    Checksum:iC6234F3C1D7480C7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061017 mRNA. Translation: AAC36096.1.
    BC006749 mRNA. Translation: AAH06749.1.
    CCDSiCCDS19308.1.
    RefSeqiNP_033492.1. NM_009466.2.
    XP_006503925.1. XM_006503862.1.
    UniGeneiMm.344831.

    Genome annotation databases

    EnsembliENSMUST00000031103; ENSMUSP00000031103; ENSMUSG00000029201.
    GeneIDi22235.
    KEGGimmu:22235.
    UCSCiuc008xns.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF061017 mRNA. Translation: AAC36096.1 .
    BC006749 mRNA. Translation: AAH06749.1 .
    CCDSi CCDS19308.1.
    RefSeqi NP_033492.1. NM_009466.2.
    XP_006503925.1. XM_006503862.1.
    UniGenei Mm.344831.

    3D structure databases

    ProteinModelPortali O70475.
    SMRi O70475. Positions 1-466.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi O70475. 3 interactions.
    MINTi MINT-1854432.

    PTM databases

    PhosphoSitei O70475.

    Proteomic databases

    MaxQBi O70475.
    PaxDbi O70475.
    PRIDEi O70475.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000031103 ; ENSMUSP00000031103 ; ENSMUSG00000029201 .
    GeneIDi 22235.
    KEGGi mmu:22235.
    UCSCi uc008xns.1. mouse.

    Organism-specific databases

    CTDi 7358.
    MGIi MGI:1306785. Ugdh.

    Phylogenomic databases

    eggNOGi COG1004.
    HOGENOMi HOG000153773.
    HOVERGENi HBG003512.
    InParanoidi O70475.
    KOi K00012.
    OMAi RETNPTA.
    PhylomeDBi O70475.
    TreeFami TF105671.

    Enzyme and pathway databases

    UniPathwayi UPA00038 ; UER00491 .

    Miscellaneous databases

    NextBioi 302285.
    PROi O70475.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O70475.
    Bgeei O70475.
    CleanExi MM_UGDH.
    Genevestigatori O70475.

    Family and domain databases

    Gene3Di 3.40.50.720. 2 hits.
    InterProi IPR008927. 6-PGluconate_DH_C-like.
    IPR016040. NAD(P)-bd_dom.
    IPR017476. UDP-Glc/GDP-Man.
    IPR014027. UDP-Glc/GDP-Man_DH_C.
    IPR014026. UDP-Glc/GDP-Man_DH_dimer.
    IPR001732. UDP-Glc/GDP-Man_DH_N.
    IPR028356. UDPglc_DH_euk.
    [Graphical view ]
    PANTHERi PTHR11374. PTHR11374. 1 hit.
    PTHR11374:SF3. PTHR11374:SF3. 1 hit.
    Pfami PF00984. UDPG_MGDP_dh. 1 hit.
    PF03720. UDPG_MGDP_dh_C. 1 hit.
    PF03721. UDPG_MGDP_dh_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF500133. UDPglc_DH_euk. 1 hit.
    PIRSF000124. UDPglc_GDPman_dh. 1 hit.
    SMARTi SM00984. UDPG_MGDP_dh_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48179. SSF48179. 1 hit.
    SSF52413. SSF52413. 1 hit.
    TIGRFAMsi TIGR03026. NDP-sugDHase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes."
      Spicer A.P., Kaback L.A., Smith T.J., Seldin M.F.
      J. Biol. Chem. 273:25117-25124(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Mammary gland.
    3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-474, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    4. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    5. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-474, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.
    6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
      Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
      Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
      Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiUGDH_MOUSE
    AccessioniPrimary (citable) accession number: O70475
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: December 15, 1998
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Allosteric enzyme, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3