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Protein

UDP-glucose 6-dehydrogenase

Gene

Ugdh

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.1 Publication

Catalytic activityi

UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH.1 Publication

Enzyme regulationi

UDP-alpha-D-xylose (UDX) acts as a feedback inhibitor by activating an allosteric switch.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei36 – 361NADBy similarity
Binding sitei41 – 411NADBy similarity
Binding sitei165 – 1651NADBy similarity
Active sitei276 – 2761NucleophileBy similarity
Binding sitei346 – 3461NADBy similarity
Binding sitei442 – 4421SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi11 – 166NADBy similarity
Nucleotide bindingi89 – 935NADBy similarity
Nucleotide bindingi130 – 1312NADBy similarity
Nucleotide bindingi276 – 2794NADBy similarity

GO - Molecular functioni

  1. NAD binding Source: InterPro
  2. UDP-glucose 6-dehydrogenase activity Source: CACAO

GO - Biological processi

  1. gastrulation with mouth forming second Source: MGI
  2. UDP-glucuronate biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NAD

Enzyme and pathway databases

ReactomeiREACT_324327. Formation of the active cofactor, UDP-glucuronate.
UniPathwayiUPA00038; UER00491.

Names & Taxonomyi

Protein namesi
Recommended name:
UDP-glucose 6-dehydrogenase (EC:1.1.1.22)
Short name:
UDP-Glc dehydrogenase
Short name:
UDP-GlcDH
Short name:
UDPGDH
Gene namesi
Name:Ugdh
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1306785. Ugdh.

Subcellular locationi

GO - Cellular componenti

  1. extracellular vesicular exosome Source: MGI
  2. nucleoplasm Source: MGI
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 493493UDP-glucose 6-dehydrogenasePRO_0000074061Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei107 – 1071N6-acetyllysine1 Publication
Modified residuei474 – 4741Phosphothreonine2 Publications

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiO70475.
PaxDbiO70475.
PRIDEiO70475.

PTM databases

PhosphoSiteiO70475.

Expressioni

Gene expression databases

BgeeiO70475.
CleanExiMM_UGDH.
ExpressionAtlasiO70475. baseline and differential.
GenevestigatoriO70475.

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

IntActiO70475. 3 interactions.
MINTiMINT-1854432.

Structurei

3D structure databases

ProteinModelPortaliO70475.
SMRiO70475. Positions 1-466.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni161 – 1655Substrate bindingBy similarity
Regioni220 – 2278Substrate bindingBy similarity
Regioni260 – 27314Substrate bindingBy similarityAdd
BLAST
Regioni338 – 3392Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1004.
HOGENOMiHOG000153773.
HOVERGENiHBG003512.
InParanoidiO70475.
KOiK00012.
OMAiAEEGVNH.
PhylomeDBiO70475.
TreeFamiTF105671.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028356. UDPglc_DH_euk.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PTHR11374:SF3. PTHR11374:SF3. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.

Sequencei

Sequence statusi: Complete.

O70475-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVEIKKICCI GAGYVGGPTC SVIAHMCPEI RVTVVDVNEA RINAWNSPTL
60 70 80 90 100
PIYEPGLKEV VESCRGKNLF FSTNIDDAIR EADLVFISVN TPTKTYGMGK
110 120 130 140 150
GRAADLKYIE ACARRIVQNS NGYKIVTEKS TVPVRAAESI RRIFDANTKP
160 170 180 190 200
NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI GGDETPEGQK AVRALCAVYE
210 220 230 240 250
HWVPKEKILT TNTWSSELSK LAANAFLAQR ISSINSISAL CEATGADVEE
260 270 280 290 300
VATAIGMDQR IGNKFLKASV GFGGSCFQKD VLNLVYLCEA LNLPEVARYW
310 320 330 340 350
QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS
360 370 380 390 400
IYISKYLMDE GAHLHIYDPK VPREQIVVDL SHPGVSADDQ VSRLVTISKD
410 420 430 440 450
PYEACDGAHA LVICTEWDMF KELDYERIHK KMLKPAFIFD GRRVLDGLHS
460 470 480 490
ELQTIGFQIE TIGKKVSSKR IPYTPGEIPK FSLQDPPNKK PKV
Length:493
Mass (Da):54,832
Last modified:August 1, 1998 - v1
Checksum:iC6234F3C1D7480C7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061017 mRNA. Translation: AAC36096.1.
BC006749 mRNA. Translation: AAH06749.1.
CCDSiCCDS19308.1.
RefSeqiNP_033492.1. NM_009466.2.
XP_006503925.1. XM_006503862.2.
UniGeneiMm.344831.

Genome annotation databases

EnsembliENSMUST00000031103; ENSMUSP00000031103; ENSMUSG00000029201.
GeneIDi22235.
KEGGimmu:22235.
UCSCiuc008xns.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF061017 mRNA. Translation: AAC36096.1.
BC006749 mRNA. Translation: AAH06749.1.
CCDSiCCDS19308.1.
RefSeqiNP_033492.1. NM_009466.2.
XP_006503925.1. XM_006503862.2.
UniGeneiMm.344831.

3D structure databases

ProteinModelPortaliO70475.
SMRiO70475. Positions 1-466.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70475. 3 interactions.
MINTiMINT-1854432.

PTM databases

PhosphoSiteiO70475.

Proteomic databases

MaxQBiO70475.
PaxDbiO70475.
PRIDEiO70475.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031103; ENSMUSP00000031103; ENSMUSG00000029201.
GeneIDi22235.
KEGGimmu:22235.
UCSCiuc008xns.1. mouse.

Organism-specific databases

CTDi7358.
MGIiMGI:1306785. Ugdh.

Phylogenomic databases

eggNOGiCOG1004.
HOGENOMiHOG000153773.
HOVERGENiHBG003512.
InParanoidiO70475.
KOiK00012.
OMAiAEEGVNH.
PhylomeDBiO70475.
TreeFamiTF105671.

Enzyme and pathway databases

UniPathwayiUPA00038; UER00491.
ReactomeiREACT_324327. Formation of the active cofactor, UDP-glucuronate.

Miscellaneous databases

ChiTaRSiUgdh. mouse.
NextBioi302285.
PROiO70475.
SOURCEiSearch...

Gene expression databases

BgeeiO70475.
CleanExiMM_UGDH.
ExpressionAtlasiO70475. baseline and differential.
GenevestigatoriO70475.

Family and domain databases

Gene3Di3.40.50.720. 2 hits.
InterProiIPR008927. 6-PGluconate_DH_C-like.
IPR016040. NAD(P)-bd_dom.
IPR017476. UDP-Glc/GDP-Man.
IPR014027. UDP-Glc/GDP-Man_DH_C.
IPR014026. UDP-Glc/GDP-Man_DH_dimer.
IPR001732. UDP-Glc/GDP-Man_DH_N.
IPR028356. UDPglc_DH_euk.
[Graphical view]
PANTHERiPTHR11374. PTHR11374. 1 hit.
PTHR11374:SF3. PTHR11374:SF3. 1 hit.
PfamiPF00984. UDPG_MGDP_dh. 1 hit.
PF03720. UDPG_MGDP_dh_C. 1 hit.
PF03721. UDPG_MGDP_dh_N. 1 hit.
[Graphical view]
PIRSFiPIRSF500133. UDPglc_DH_euk. 1 hit.
PIRSF000124. UDPglc_GDPman_dh. 1 hit.
SMARTiSM00984. UDPG_MGDP_dh_C. 1 hit.
[Graphical view]
SUPFAMiSSF48179. SSF48179. 1 hit.
SSF52413. SSF52413. 1 hit.
TIGRFAMsiTIGR03026. NDP-sugDHase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes."
    Spicer A.P., Kaback L.A., Smith T.J., Seldin M.F.
    J. Biol. Chem. 273:25117-25124(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Mammary gland.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-474, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  4. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
    Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
    J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  5. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
    Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
    Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-474, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.
  6. "SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways."
    Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y., Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.
    Mol. Cell 50:919-930(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-107, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Embryonic fibroblast.

Entry informationi

Entry nameiUGDH_MOUSE
AccessioniPrimary (citable) accession number: O70475
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 15, 1998
Last sequence update: August 1, 1998
Last modified: April 29, 2015
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.