Reviewed, UniProtKB/Swiss-Prot O70475 (UGDH_MOUSE)

Names and origin

Protein names

Recommended name:
    UDP-glucose 6-dehydrogenase
      Short name=UDP-Glc dehydrogenase
      Short name=UDP-GlcDH
      Short name=UDPGDH
    EC=1.1.1.22

Gene names

Name:

Ugdh

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	493 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate. Ref.1
Catalytic activity	UDP-glucose + 2 NAD+ + H2O = UDP-glucuronate + 2 NADH. Ref.1
Pathway	Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.
Subunit structure	Homohexamer By similarity.
Sequence similarities	Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Biological process	gastrulation with mouth forming second Inferred from mutant phenotype. Source: MGI oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro UDP-glucose 6-dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 493	493	UDP-glucose 6-dehydrogenase		PRO_0000074061
Regions
	Nucleotide binding	11 – 16	6	NAD By similarity
	Nucleotide binding	89 – 93	5	NAD By similarity
	Nucleotide binding	130 – 131	2	NAD By similarity
	Nucleotide binding	276 – 279	4	NAD By similarity
	Region	161 – 165	5	Substrate binding By similarity
	Region	220 – 227	8	Substrate binding By similarity
	Region	260 – 273	14	Substrate binding By similarity
	Region	338 – 339	2	Substrate binding By similarity
Sites
	Active site	276	1	Nucleophile By similarity
	Binding site	36	1	NAD By similarity
	Binding site	41	1	NAD By similarity
	Binding site	165	1	NAD By similarity
	Binding site	346	1	NAD By similarity
	Binding site	442	1	Substrate By similarity
Amino acid modifications
	Modified residue	107	1	N6-acetyllysine By similarity
	Modified residue	173	1	N6-acetyllysine By similarity
	Modified residue	434	1	N6-acetyllysine By similarity
	Modified residue	473	1	Phosphotyrosine Ref.3
	Modified residue	474	1	Phosphothreonine Ref.3 Ref.4 Ref.5

Sequences

	Sequence		Length	Mass (Da)
	O70475-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: C6234F3C1D7480C7	FASTA	493	54,832
	10 20 30 40 50 60 MVEIKKICCI GAGYVGGPTC SVIAHMCPEI RVTVVDVNEA RINAWNSPTL PIYEPGLKEV 70 80 90 100 110 120 VESCRGKNLF FSTNIDDAIR EADLVFISVN TPTKTYGMGK GRAADLKYIE ACARRIVQNS 130 140 150 160 170 180 NGYKIVTEKS TVPVRAAESI RRIFDANTKP NLNLQVLSNP EFLAEGTAIK DLKNPDRVLI 190 200 210 220 230 240 GGDETPEGQK AVRALCAVYE HWVPKEKILT TNTWSSELSK LAANAFLAQR ISSINSISAL 250 260 270 280 290 300 CEATGADVEE VATAIGMDQR IGNKFLKASV GFGGSCFQKD VLNLVYLCEA LNLPEVARYW 310 320 330 340 350 360 QQVIDMNDYQ RRRFASRIID SLFNTVTDKK IAILGFAFKK DTGDTRESSS IYISKYLMDE 370 380 390 400 410 420 GAHLHIYDPK VPREQIVVDL SHPGVSADDQ VSRLVTISKD PYEACDGAHA LVICTEWDMF 430 440 450 460 470 480 KELDYERIHK KMLKPAFIFD GRRVLDGLHS ELQTIGFQIE TIGKKVSSKR IPYTPGEIPK 490 FSLQDPPNKK PKV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and characterization of the human and mouse UDP-glucose dehydrogenase genes." Spicer A.P., Kaback L.A., Smith T.J., Seldin M.F. J. Biol. Chem. 273:25117-25124(1998) [PubMed: 9737970] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, FUNCTION.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: FVB/N. Tissue: Mammary gland.
[3]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-473 AND THR-474, MASS SPECTROMETRY. Tissue: Liver.
[4]	"Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis." Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H. J. Proteome Res. 7:3957-3967(2008) [PubMed: 18630941] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-474, MASS SPECTROMETRY. Tissue: Liver.
[5]	"Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry." Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J. Mol. Cell. Proteomics 8:904-912(2009) [PubMed: 19131326] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-474, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF061017 mRNA. Translation: AAC36096.1. BC006749 mRNA. Translation: AAH06749.1.
IPI	IPI00118344.
RefSeq	NP_033492.1.
UniGene	Mm.344831
3D structure databases
SMR	O70475. Positions 1-466.
ModBase	Search...
Protein-protein interaction databases
STRING	O70475.
PTM databases
PhosphoSite	O70475.
Proteomic databases
PRIDE	O70475.
Genome annotation databases
Ensembl	ENSMUST00000031103; ENSMUSP00000031103; ENSMUSG00000029201; Mus musculus. [Genome view]
GeneID	22235.
KEGG	mmu:22235.
UCSC	uc008xns.1. mouse.
Organism-specific databases
CTD	22235.
MGI	MGI:1306785. Ugdh.
Phylogenomic databases
eggNOG	roNOG08820.
HOGENOM	HBG400967.
HOVERGEN	O70475.
InParanoid	O70475.
OMA	ARINAWN.
PhylomeDB	O70475.
Enzyme and pathway databases
BRENDA	1.1.1.22. 244.
Gene expression databases
ArrayExpress	O70475.
Bgee	O70475.
CleanEx	MM_UGDH.
Genevestigator	O70475.
GermOnline	ENSMUSG00000029201. Mus musculus.
Family and domain databases
InterPro	IPR008927. 6-PGluconate_DH_C-like. IPR016040. NAD(P)-bd_dom. IPR017476. Nucleotide_sugar_DH. IPR014027. UDP-Glc/GDP-Man_DH_C. IPR014026. UDP-Glc/GDP-Man_DH_dimer. IPR014028. UDP-Glc/GDP-Man_DH_dimer-bd. IPR001732. UDP-Glc/GDP-Man_DH_N. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:3.40.50.1870. UDP-Glc/GDP-Man_DH_C. 1 hit.
PANTHER	PTHR11374. UDPG_MGDP_DH_Creg. 1 hit.
Pfam	PF00984. UDPG_MGDP_dh. 1 hit. PF03720. UDPG_MGDP_dh_C. 1 hit. PF03721. UDPG_MGDP_dh_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR03026. NDP-sugDHase. 1 hit.
ProtoNet	Search...
Other Resources
NextBio	302285.
SOURCE	Search...

Entry information

Entry name

UGDH_MOUSE

Accession

Primary (citable) accession number: O70475

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)