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Protein

Docking protein 2

Gene

Dok2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK2 may modulate the cellular proliferation induced by IL-4, as well as IL-2 and IL-3. May be involved in modulating Bcr-Abl signaling. Attenuates EGF-stimulated MAP kinase activation.

GO - Molecular functioni

  • insulin receptor binding Source: InterPro
  • signal transducer activity, downstream of receptor Source: MGI
  • transmembrane receptor protein tyrosine kinase adaptor activity Source: MGI

GO - Biological processi

  • MAPK cascade Source: MGI
  • Ras protein signal transduction Source: MGI
  • transmembrane receptor protein tyrosine kinase signaling pathway Source: MGI

Enzyme and pathway databases

ReactomeiR-MMU-210993. Tie2 Signaling.
R-MMU-8853659. RET signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Docking protein 2
Alternative name(s):
Dok-related protein
Short name:
Dok-R
Downstream of tyrosine kinase 2
IL-four receptor-interacting protein
Short name:
FRIP
p56(dok-2)
Gene namesi
Name:Dok2
Synonyms:Frip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1332623. Dok2.

Subcellular locationi

GO - Cellular componenti

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi276Y → F: No loss of binding to SH2 domain of RASGAP or NCK. Complete loss of binding to SH2 domain of RASGAP; when associated with F-304. 1 Publication1
Mutagenesisi304Y → F: No loss of binding to SH2 domain of RASGAP. Complete loss of binding to SH2 domain of RASGAP; when associated with F-276. 1 Publication1
Mutagenesisi351Y → F: No loss of binding to SH2 domain of RASGAP. Complete loss of binding to SH2 domain of NCK. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001872711 – 412Docking protein 2Add BLAST412

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei276Phosphotyrosine1 Publication1
Modified residuei304Phosphotyrosine1 Publication1
Modified residuei351Phosphotyrosine1 Publication1

Post-translational modificationi

On immunoreceptor stimulation, phosphorylated on C-terminal tyrosine residues. Phosphorylation on Tyr-351 is required for binding to the SH2 domain of NCK. Phosphorylation on both Tyr-276 and Tyr-304 is required for interaction with RASGAP. Phosphorylated on tyrosine residues by TEK/TIE2.4 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiO70469.
PaxDbiO70469.
PeptideAtlasiO70469.
PRIDEiO70469.

PTM databases

iPTMnetiO70469.
PhosphoSitePlusiO70469.

Expressioni

Tissue specificityi

Highly expressed in spleen and lung.2 Publications

Developmental stagei

During embryonic liver development, expressed in the islands of cells, consistent with an expression in hematopoietic precursors.1 Publication

Gene expression databases

BgeeiENSMUSG00000022102.
CleanExiMM_DOK2.
ExpressionAtlasiO70469. baseline and differential.
GenevisibleiO70469. MM.

Interactioni

Subunit structurei

Interacts with phosphorylated RASGAP and EGFR. Interacts with RET and NCK. Interacts (via PH domain) with TEK/TIE2 (tyrosine phosphorylated).3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi199268. 6 interactors.
IntActiO70469. 10 interactors.
MINTiMINT-100549.
STRINGi10090.ENSMUSP00000022698.

Structurei

3D structure databases

ProteinModelPortaliO70469.
SMRiO70469.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini7 – 117PHPROSITE-ProRule annotationAdd BLAST111
Domaini149 – 254IRS-type PTBPROSITE-ProRule annotationAdd BLAST106

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi252 – 307Pro-richAdd BLAST56
Compositional biasi326 – 369Pro-richAdd BLAST44

Domaini

PTB domain mediates receptor interaction.

Sequence similaritiesi

Belongs to the DOK family. Type A subfamily.Curated

Phylogenomic databases

eggNOGiKOG4047. Eukaryota.
ENOG410XS2S. LUCA.
GeneTreeiENSGT00730000110348.
HOGENOMiHOG000112245.
HOVERGENiHBG018962.
InParanoidiO70469.
KOiK20234.
OMAiEYAVPFD.
OrthoDBiEOG091G075T.
PhylomeDBiO70469.
TreeFamiTF324994.

Family and domain databases

Gene3Di2.30.29.30. 2 hits.
InterProiView protein in InterPro
IPR002404. IRS_PTB.
IPR011993. PH_dom-like.
IPR001849. PH_domain.
PfamiView protein in Pfam
PF02174. IRS. 1 hit.
PF00169. PH. 1 hit.
SMARTiView protein in SMART
SM00233. PH. 1 hit.
SM00310. PTBI. 1 hit.
SUPFAMiSSF50729. SSF50729. 2 hits.
PROSITEiView protein in PROSITE
PS51064. IRS_PTB. 1 hit.
PS50003. PH_DOMAIN. 1 hit.

Sequencei

Sequence statusi: Complete.

O70469-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVRMEEPAVK QGFLHLQQQQ TFGKKWRRFA AVLYGESGCA LARLELQDVP
60 70 80 90 100
EKTRRGEATR KVVRLSDCLR VAEVGSEASS PRDTSAFILE TKERLYLLAA
110 120 130 140 150
PSAERSDWIQ AICLLAFPGQ RKGSPGLEEK SGSPCMEENE LYSSSTTGLC
160 170 180 190 200
KEYMVTIRPT EASERCRLRG SYTLRTGVSA LELWGGPEPG TQLYDWPYRF
210 220 230 240 250
LRRFGRDKAT FSFEAGRRCL SGEGNFEFET RHGNEIFQAL EKVIAVQKNA
260 270 280 290 300
TPSGPPSLPA TGPMMPTVLP RPESPYSRPH DSLPSPSPGT LVPGMRPGAP
310 320 330 340 350
EGEYAVPFDT VAHSLRKSFR GLLTGPPPHL PDPLYDSIQE DPGAPLPDHI
360 370 380 390 400
YDEPEGVAAL SLYDRTQRPS GETWREQATA DGGPSSLQQD SSVPDWPQAT
410
EYDNVILKKG PK
Length:412
Mass (Da):45,522
Last modified:August 1, 1998 - v1
Checksum:i02AC02530DBED053
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245A → T in AAH04590 (PubMed:15489334).Curated1
Sequence conflicti330L → F in AAC13266 (PubMed:9478921).Curated1
Sequence conflicti347P → T in AAC13266 (PubMed:9478921).Curated1
Sequence conflicti412Missing in AAH04590 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF030627 mRNA. Translation: AAC31315.1.
AF035117 mRNA. Translation: AAC13266.1.
AF059583 mRNA. Translation: AAC78606.1.
BC004590 mRNA. Translation: AAH04590.1.
CCDSiCCDS27262.1.
RefSeqiNP_034201.1. NM_010071.2.
UniGeneiMm.243323.

Genome annotation databases

EnsembliENSMUST00000022698; ENSMUSP00000022698; ENSMUSG00000022102.
GeneIDi13449.
KEGGimmu:13449.
UCSCiuc007uoy.1. mouse.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiDOK2_MOUSE
AccessioniPrimary (citable) accession number: O70469
Secondary accession number(s): O70272, Q99KL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: August 1, 1998
Last modified: June 7, 2017
This is version 131 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families