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Protein

Protein arginine N-methyltransferase 3

Gene

Prmt3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues in some proteins.1 Publication

Enzyme regulationi

Inhibited by N-ethylmaleimide and high concentrations of zinc chloride.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei227S-adenosyl-L-methionineBy similarity1
Binding sitei236S-adenosyl-L-methionine1
Binding sitei260S-adenosyl-L-methionine; via carbonyl oxygen1
Binding sitei282S-adenosyl-L-methionine1
Binding sitei311S-adenosyl-L-methionine1
Active sitei3261 Publication1
Active sitei3351 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri46 – 69C2H2-typeAdd BLAST24

GO - Molecular functioni

  • histone-arginine N-methyltransferase activity Source: GO_Central
  • metal ion binding Source: UniProtKB-KW
  • modified amino acid binding Source: RGD
  • protein-arginine N-methyltransferase activity Source: RGD
  • protein-arginine omega-N asymmetric methyltransferase activity Source: RGD
  • S-adenosylmethionine-dependent methyltransferase activity Source: RGD

GO - Biological processi

  • dendritic spine morphogenesis Source: RGD
  • peptidyl-arginine methylation Source: RGD
  • peptidyl-arginine methylation, to asymmetrical-dimethyl arginine Source: RGD
  • regulation of transcription, DNA-templated Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Ligandi

Metal-binding, S-adenosyl-L-methionine, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein arginine N-methyltransferase 3 (EC:2.1.1.-)
Alternative name(s):
Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3
Gene namesi
Name:Prmt3
Synonyms:Hrmt1l3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 1

Organism-specific databases

RGDi620413. Prmt3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: RGD
  • cytosol Source: GO_Central
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002123282 – 528Protein arginine N-methyltransferase 3Add BLAST527

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylcysteineBy similarity1
Modified residuei22PhosphoserineBy similarity1
Modified residuei24PhosphoserineBy similarity1
Modified residuei169PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO70467.
PRIDEiO70467.

PTM databases

iPTMnetiO70467.
PhosphoSitePlusiO70467.

Expressioni

Gene expression databases

BgeeiENSRNOG00000014829.
ExpressionAtlasiO70467. baseline and differential.
GenevisibleiO70467. RN.

Interactioni

Subunit structurei

Monomer or homodimer. Interacts with the 40S ribosomal protein RPS2 (By similarity). Interacts with EPB41L3; this inhibits methylation of target proteins.By similarity2 Publications

Protein-protein interaction databases

IntActiO70467. 1 interactor.
STRINGi10116.ENSRNOP00000020853.

Structurei

Secondary structure

1528
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi210 – 212Combined sources3
Helixi215 – 220Combined sources6
Beta strandi222 – 224Combined sources3
Helixi225 – 231Combined sources7
Helixi234 – 246Combined sources13
Helixi248 – 250Combined sources3
Turni251 – 253Combined sources3
Beta strandi255 – 260Combined sources6
Helixi265 – 273Combined sources9
Beta strandi276 – 284Combined sources9
Helixi286 – 296Combined sources11
Turni300 – 302Combined sources3
Beta strandi303 – 308Combined sources6
Turni310 – 312Combined sources3
Beta strandi316 – 318Combined sources3
Beta strandi320 – 324Combined sources5
Turni333 – 335Combined sources3
Helixi337 – 348Combined sources12
Beta strandi349 – 357Combined sources9
Beta strandi359 – 367Combined sources9
Helixi370 – 376Combined sources7
Helixi378 – 381Combined sources4
Helixi389 – 394Combined sources6
Turni395 – 397Combined sources3
Beta strandi400 – 402Combined sources3
Helixi406 – 408Combined sources3
Beta strandi414 – 420Combined sources7
Turni421 – 423Combined sources3
Helixi426 – 429Combined sources4
Beta strandi430 – 439Combined sources10
Beta strandi443 – 456Combined sources14
Beta strandi464 – 467Combined sources4
Beta strandi479 – 490Combined sources12
Beta strandi495 – 504Combined sources10
Beta strandi511 – 518Combined sources8
Beta strandi521 – 527Combined sources7

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F3LX-ray2.03A208-528[»]
ProteinModelPortaliO70467.
SMRiO70467.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO70467.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini214 – 528SAM-dependent MTase PRMT-typePROSITE-ProRule annotationAdd BLAST315

Domaini

The zinc-finger is responsible for substrate specificity.

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. Protein arginine N-methyltransferase family.PROSITE-ProRule annotation
Contains 1 C2H2-type zinc finger.Curated
Contains 1 SAM-dependent MTase PRMT-type domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri46 – 69C2H2-typeAdd BLAST24

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG1499. Eukaryota.
ENOG410XQYH. LUCA.
GeneTreeiENSGT00550000074406.
HOGENOMiHOG000198521.
HOVERGENiHBG001793.
InParanoidiO70467.
KOiK11436.
PhylomeDBiO70467.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025799. Arg_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11006. PTHR11006. 2 hits.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51678. SAM_MT_PRMT. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70467-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MCSLAAGNGQ GAELGPEPLE LSDSGDDAGW EDEDADAEPA QGRQHTPCLF
60 70 80 90 100
CDRLFRSAEE TFSHCKLEHQ FNIDGMVHKH GLEFYGYIKL INFIRLKNPT
110 120 130 140 150
VEYMNSIYNP VPWDKDEYLK PVLEDDLLLQ FDVEDLYEPV SAPFTYPNGL
160 170 180 190 200
SENTSAVEKL KLMEARALSA EAALARARED LQKMKQFAQD FVMNVDVRTC
210 220 230 240 250
SSTTTIADLQ EDEDGVYFSS YGHYGIHEEM LKDKVRTESY RDFIYQNPHI
260 270 280 290 300
FKDKVVLDVG CGTGILSMFA AKAGAKKVIA VDQSEILYQA MDIIRLNKLE
310 320 330 340 350
DTIVLIKGKI EEVSLPVEKV DVIISEWMGY FLLFESMLDS VLYAKSKYLA
360 370 380 390 400
KGGSVYPDIC TISLVAVSDV SKHADRIAFW DDVYGFNMSC MKKAVIPEAV
410 420 430 440 450
VEVVDHKTLI SDPCDIKHID CHTTSISDLE FSSDFTLRTT KTAMCTAVAG
460 470 480 490 500
YFDIYFEKNC HNRVVFSTGP QSTKTHWKQT IFLLEKPFPV KAGEALKGKI
510 520
TVHKNKKDPR SLIVTLTLNS STQTYSLQ
Length:528
Mass (Da):59,420
Last modified:August 1, 1998 - v1
Checksum:iB25D627902594B39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059530 mRNA. Translation: AAC40158.1.
RefSeqiNP_446009.1. NM_053557.1.
UniGeneiRn.33389.

Genome annotation databases

EnsembliENSRNOT00000020853; ENSRNOP00000020853; ENSRNOG00000014829.
GeneIDi89820.
KEGGirno:89820.
UCSCiRGD:620413. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF059530 mRNA. Translation: AAC40158.1.
RefSeqiNP_446009.1. NM_053557.1.
UniGeneiRn.33389.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F3LX-ray2.03A208-528[»]
ProteinModelPortaliO70467.
SMRiO70467.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70467. 1 interactor.
STRINGi10116.ENSRNOP00000020853.

PTM databases

iPTMnetiO70467.
PhosphoSitePlusiO70467.

Proteomic databases

PaxDbiO70467.
PRIDEiO70467.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000020853; ENSRNOP00000020853; ENSRNOG00000014829.
GeneIDi89820.
KEGGirno:89820.
UCSCiRGD:620413. rat.

Organism-specific databases

CTDi10196.
RGDi620413. Prmt3.

Phylogenomic databases

eggNOGiKOG1499. Eukaryota.
ENOG410XQYH. LUCA.
GeneTreeiENSGT00550000074406.
HOGENOMiHOG000198521.
HOVERGENiHBG001793.
InParanoidiO70467.
KOiK11436.
PhylomeDBiO70467.

Miscellaneous databases

EvolutionaryTraceiO70467.
PROiO70467.

Gene expression databases

BgeeiENSRNOG00000014829.
ExpressionAtlasiO70467. baseline and differential.
GenevisibleiO70467. RN.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR025799. Arg_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PANTHERiPTHR11006. PTHR11006. 2 hits.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS51678. SAM_MT_PRMT. 1 hit.
PS00028. ZINC_FINGER_C2H2_1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANM3_RAT
AccessioniPrimary (citable) accession number: O70467
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 11, 2001
Last sequence update: August 1, 1998
Last modified: November 2, 2016
This is version 135 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.