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Protein

14-3-3 protein sigma

Gene

Sfn

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. When bound to KRT17, regulates protein synthesis and epithelial cell growth by stimulating Akt/mTOR pathway. May also regulate MDM2 autoubiquitination and degradation and thereby activate p53/TP53.1 Publication

GO - Molecular functioni

  • phosphoprotein binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

GO - Biological processi

  • establishment of skin barrier Source: UniProtKB
  • intrinsic apoptotic signaling pathway in response to DNA damage Source: MGI
  • keratinization Source: MGI
  • keratinocyte development Source: MGI
  • keratinocyte differentiation Source: MGI
  • negative regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
  • negative regulation of keratinocyte proliferation Source: MGI
  • positive regulation of cell growth Source: UniProtKB
  • positive regulation of epidermal cell differentiation Source: UniProtKB
  • positive regulation of protein export from nucleus Source: UniProtKB
  • regulation of cell cycle Source: MGI
  • regulation of cyclin-dependent protein serine/threonine kinase activity Source: MGI
  • regulation of epidermal cell division Source: UniProtKB
  • release of cytochrome c from mitochondria Source: MGI
  • skin development Source: MGI
Complete GO annotation...

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-MMU-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein sigma
Alternative name(s):
Stratifin
Gene namesi
Name:Sfn
Synonyms:Mkrn3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1891831. Sfn.

Subcellular locationi

  • Cytoplasm 1 Publication
  • Nucleus 1 Publication
  • Secreted By similarity

  • Note: May be secreted by a non-classical secretory pathway.By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • cytoplasmic vesicle membrane Source: Reactome
  • cytosol Source: Reactome
  • extracellular exosome Source: MGI
  • nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus, Secreted

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL1909483.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 24824814-3-3 protein sigmaPRO_0000058644Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei5 – 51PhosphoserineBy similarity
Modified residuei74 – 741PhosphoserineBy similarity
Modified residuei248 – 2481PhosphoserineBy similarity

Post-translational modificationi

Ubiquitinated. Ubiquitination by RFFL induces proteasomal degradation and indirectly regulates p53/TP53 activation (By similarity).By similarity

Keywords - PTMi

Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiO70456.
MaxQBiO70456.
PaxDbiO70456.
PeptideAtlasiO70456.
PRIDEiO70456.

PTM databases

iPTMnetiO70456.
PhosphoSiteiO70456.

Expressioni

Tissue specificityi

Expressed in the basal layer of skin epithelium and in outer root sheath of hair follicle.1 Publication

Inductioni

Induced in damaged or stressed epidermis.1 Publication

Gene expression databases

BgeeiENSMUSG00000047281.
CleanExiMM_MKRN3.
MM_SFN.
GenevisibleiO70456. MM.

Interactioni

Subunit structurei

Homodimer. Found in a complex with XPO7, EIF4A1, ARHGAP1, VPS26A, VPS29 and VPS35. Interacts with GAB2 (By similarity). Interacts with KRT17. Interacts with SAMSN1. Interacts with SRPK2 (By similarity). Interacts with COPS6 (By similarity). Interacts with RFWD2; this interaction leads to proteasomal degradation (By similarity).Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei56 – 561Interaction with phosphoserine on interacting proteinBy similarity
Sitei129 – 1291Interaction with phosphoserine on interacting proteinBy similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Krt17Q9QWL73EBI-1544118,EBI-309015

GO - Molecular functioni

  • phosphoprotein binding Source: UniProtKB
  • protein kinase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi207743. 5 interactions.
IntActiO70456. 4 interactions.
MINTiMINT-240814.
STRINGi10090.ENSMUSP00000050374.

Structurei

3D structure databases

ProteinModelPortaliO70456.
SMRiO70456. Positions 1-231.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiO70456.
KOiK06644.
OMAiGPEVQEY.
OrthoDBiEOG091G0VKY.
TreeFamiTF102003.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70456-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERASLIQKA KLAEQAERYE DMAAFMKSAV EKGEELSCEE RNLLSVAYKN
60 70 80 90 100
VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VKEYREKVET ELRGVCDTVL
110 120 130 140 150
GLLDSHLIKG AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA
160 170 180 190 200
YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE
210 220 230 240
AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADSAGEEGG EAPEEPQS
Length:248
Mass (Da):27,706
Last modified:June 28, 2011 - v2
Checksum:iC1C40905824B3F48
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti244 – 2485EEPQS → DDPHI in AAC14344 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058798 mRNA. Translation: AAC14344.1.
AK146490 mRNA. Translation: BAE27209.1.
AK169358 mRNA. Translation: BAE41107.1.
AL627228 Genomic DNA. Translation: CAM14836.1.
CH466552 Genomic DNA. Translation: EDL30056.1.
CCDSiCCDS18754.1.
RefSeqiNP_061224.2. NM_018754.2.
UniGeneiMm.44482.

Genome annotation databases

EnsembliENSMUST00000057311; ENSMUSP00000050374; ENSMUSG00000047281.
GeneIDi55948.
KEGGimmu:55948.
UCSCiuc008vdc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058798 mRNA. Translation: AAC14344.1.
AK146490 mRNA. Translation: BAE27209.1.
AK169358 mRNA. Translation: BAE41107.1.
AL627228 Genomic DNA. Translation: CAM14836.1.
CH466552 Genomic DNA. Translation: EDL30056.1.
CCDSiCCDS18754.1.
RefSeqiNP_061224.2. NM_018754.2.
UniGeneiMm.44482.

3D structure databases

ProteinModelPortaliO70456.
SMRiO70456. Positions 1-231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi207743. 5 interactions.
IntActiO70456. 4 interactions.
MINTiMINT-240814.
STRINGi10090.ENSMUSP00000050374.

Chemistry

ChEMBLiCHEMBL1909483.

PTM databases

iPTMnetiO70456.
PhosphoSiteiO70456.

Proteomic databases

EPDiO70456.
MaxQBiO70456.
PaxDbiO70456.
PeptideAtlasiO70456.
PRIDEiO70456.

Protocols and materials databases

DNASUi55948.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000057311; ENSMUSP00000050374; ENSMUSG00000047281.
GeneIDi55948.
KEGGimmu:55948.
UCSCiuc008vdc.1. mouse.

Organism-specific databases

CTDi2810.
MGIiMGI:1891831. Sfn.

Phylogenomic databases

eggNOGiKOG0841. Eukaryota.
COG5040. LUCA.
GeneTreeiENSGT00760000119116.
HOGENOMiHOG000240379.
HOVERGENiHBG050423.
InParanoidiO70456.
KOiK06644.
OMAiGPEVQEY.
OrthoDBiEOG091G0VKY.
TreeFamiTF102003.

Enzyme and pathway databases

ReactomeiR-MMU-111447. Activation of BAD and translocation to mitochondria.
R-MMU-1445148. Translocation of GLUT4 to the plasma membrane.
R-MMU-5625740. RHO GTPases activate PKNs.
R-MMU-5628897. TP53 Regulates Metabolic Genes.
R-MMU-6804114. TP53 Regulates Transcription of Genes Involved in G2 Cell Cycle Arrest.
R-MMU-75035. Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex.

Miscellaneous databases

PROiO70456.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000047281.
CleanExiMM_MKRN3.
MM_SFN.
GenevisibleiO70456. MM.

Family and domain databases

InterProiIPR000308. 14-3-3.
IPR023409. 14-3-3_CS.
IPR023410. 14-3-3_domain.
[Graphical view]
PANTHERiPTHR18860. PTHR18860. 1 hit.
PfamiPF00244. 14-3-3. 1 hit.
[Graphical view]
PIRSFiPIRSF000868. 14-3-3. 1 hit.
PRINTSiPR00305. 1433ZETA.
SMARTiSM00101. 14_3_3. 1 hit.
[Graphical view]
SUPFAMiSSF48445. SSF48445. 1 hit.
PROSITEiPS00796. 1433_1. 1 hit.
PS00797. 1433_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry namei1433S_MOUSE
AccessioniPrimary (citable) accession number: O70456
Secondary accession number(s): Q3TEZ1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: June 28, 2011
Last modified: September 7, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

14-3-3 proteins have been shown to be PKC activators, but this effect could be non-specific and only due to the acidic nature of the protein.

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.