O70453 (HMOX3_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Putative heme oxygenase 3
Short name=HO-3
EC=1.14.99.3

Gene names

Name:

Hmox3

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	290 AA.
Sequence status	Complete.
Protein existence	Uncertain

General annotation (Comments)

Function	Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Heme oxygenase 3 could be implicated in some heme-dependent regulatory role in the cell.
Catalytic activity	Heme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.
Tissue specificity	Found in the spleen, liver, thymus, prostate, heart, kidney, brain and testis.
Sequence similarities	Belongs to the heme oxygenase family. Contains 2 HRM (heme regulatory motif) repeats.
Caution	Could be the product of a pseudogene. Encoded by a single-exon gene, absent in other mammals, including mouse, and not supported by EST data. Expression could not be detected at the mRNA level in a genomic DNA-free liver library, nor at the protein level in kidney (Ref.2).

Ontologies

Keywords
Domain	Repeat
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	heme oxidation Inferred from electronic annotation. Source: InterPro
Molecular_function	heme oxygenase (decyclizing) activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 290	290	Putative heme oxygenase 3		PRO_0000209695
Regions
	Repeat	238 – 243	6	HRM 1
	Repeat	255 – 260	6	HRM 2

Sequences

	Sequence		Length	Mass (Da)
	O70453 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 6600235CE8485829	FASTA	290	32,592
	10 20 30 40 50 60 MSSEVETAEA VDESEKNSMA SEKENHSKIA DFSDLLKEGT KEADDRAENT QFVKDFLKGN 70 80 90 100 110 120 IKKELFKLAT TALSYSAPEE EMDSLTKDME YFFGENWEEK VKCSEAAQTY VDQIHYVGQN 130 140 150 160 170 180 EPEHLVAHTY STYMGGNLSG DQVLKKETQP VPFTREGTQF YLFEHVDNAK QFKLFYCARL 190 200 210 220 230 240 NALDLNLKTK ERIVEEATKA FEYNMQIFSE LDQAGSIPVR ETLKNGLSIL DGKGGVCKCP 250 260 270 280 290 FNAAQPDKGT LGGSNCPFQM SMALLRKPNL QLILVASMAL VAGLLAWYYM

References

[1]	"Isolation and characterization of a cDNA from the rat brain that encodes hemoprotein heme oxygenase-3." McCoubrey W.K. Jr., Huang T.J., Maines M.D. Eur. J. Biochem. 247:725-732(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Characterization of rat heme oxygenase-3 gene. Implication of processed pseudogenes derived from heme oxygenase-2 gene." Hayashi S., Omata Y., Sakamoto H., Higashimoto Y., Hara T., Sagara Y., Noguchi M. Gene 336:241-250(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION AS A PSEUDOGENE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF058787 mRNA. Translation: AAC14142.1.
IPI	IPI00198589.
UniGene	Rn.133155.
3D structure databases
ProteinModelPortal	O70453.
SMR	O70453. Positions 29-222.
ModBase	Search...
Proteomic databases
PRIDE	O70453.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
RGD	1565339. Hmox3.
Phylogenomic databases
HOVERGEN	HBG005982.
Gene expression databases
Genevestigator	O70453.
Family and domain databases
Gene3D	1.20.910.10. 2 hits.
InterPro	IPR002051. Haem_Oase. IPR016053. Haem_Oase-like. IPR016084. Haem_Oase-like_multi-hlx. IPR018207. Haem_oxygenase_CS. [Graphical view]
PANTHER	PTHR10720. PTHR10720. 1 hit.
Pfam	PF01126. Heme_oxygenase. 1 hit. [Graphical view]
PIRSF	PIRSF000343. Haem_Oase. 1 hit.
SUPFAM	SSF48613. Heme_oxygenase. 1 hit.
PROSITE	PS00593. HEME_OXYGENASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	13948106.

Entry information

Entry name

HMOX3_RAT

Accession

Primary (citable) accession number: O70453

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 1998
Last sequence update:	August 1, 1998
Last modified:	April 3, 2013
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program