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Protein

Guanine nucleotide-binding protein G(z) subunit alpha

Gene

Gnaz

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi47 – 471MagnesiumBy similarity
Metal bindingi182 – 1821MagnesiumBy similarity
Binding sitei327 – 3271GTP; via amide nitrogenBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi40 – 478GTPBy similarity
Nucleotide bindingi176 – 1827GTPBy similarity
Nucleotide bindingi201 – 2055GTPBy similarity
Nucleotide bindingi270 – 2734GTPBy similarity

GO - Molecular functioni

  1. G-protein beta/gamma-subunit complex binding Source: GO_Central
  2. G-protein coupled serotonin receptor binding Source: GO_Central
  3. GTPase activity Source: GO_Central
  4. GTP binding Source: UniProtKB-KW
  5. metal ion binding Source: UniProtKB-KW
  6. signal transducer activity Source: GO_Central

GO - Biological processi

  1. adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway Source: Ensembl
  2. adenylate cyclase-modulating G-protein coupled receptor signaling pathway Source: GO_Central
  3. G-protein coupled receptor signaling pathway Source: MGI
  4. GTP catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Keywords - Ligandi

GTP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_205726. G-protein activation.
REACT_242871. G alpha (s) signalling events.
REACT_250376. G alpha (i) signalling events.
REACT_253342. PLC beta mediated events.
REACT_258013. G alpha (z) signalling events.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(z) subunit alpha
Alternative name(s):
G(x) alpha chain
Gz-alpha
Gene namesi
Name:Gnaz
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 10

Organism-specific databases

MGIiMGI:95780. Gnaz.

Subcellular locationi

GO - Cellular componenti

  1. cytosol Source: Ensembl
  2. extracellular vesicular exosome Source: MGI
  3. heterotrimeric G-protein complex Source: GO_Central
  4. membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedCurated
Chaini2 – 355354Guanine nucleotide-binding protein G(z) subunit alphaPRO_0000203697Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi2 – 21N-myristoyl glycineBy similarity
Lipidationi3 – 31S-palmitoyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Myristate, Palmitate

Proteomic databases

MaxQBiO70443.
PaxDbiO70443.
PRIDEiO70443.

PTM databases

PhosphoSiteiO70443.

Expressioni

Gene expression databases

BgeeiO70443.
ExpressionAtlasiO70443. baseline and differential.
GenevestigatoriO70443.

Interactioni

Subunit structurei

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Protein-protein interaction databases

IntActiO70443. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliO70443.
SMRiO70443. Positions 6-349.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the G-alpha family. G(i/o/t/z) subfamily.Curated

Phylogenomic databases

eggNOGiNOG282619.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiO70443.
KOiK04535.
OMAiYDLRLYE.
OrthoDBiEOG72C50B.
PhylomeDBiO70443.
TreeFamiTF300673.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70443-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGCRQSSEEK EAARRSRRID RHLRSESQRQ RREIKLLLLG TSNSGKSTIV
60 70 80 90 100
KQMKIIHSGG FNLDACKEYK PLIIYNAIDS LTRIIRALAA LKIDFHNPDR
110 120 130 140 150
AYDAVQLFAL TGPAESKGEI TPELLGVMRR LWADPGAQAC FGRSSEYHLE
160 170 180 190 200
DNAAYYLNDL ERIAAPDYIP TVEDILRSRD MTTGIVENKF TFKELTFKMV
210 220 230 240 250
DVGGQRSERK KWIHCFEGVT AIIFCVELSG YDLKLYEDNQ TSRMAESLRL
260 270 280 290 300
FDSICNNNWF INTSLILFLN KKDLLAEKIR RIPLSVCFPE YKGQNTYEEA
310 320 330 340 350
AVYIQRQFED LNRNKETKEI YSHFTCATDT SNIQFVFDAV TDVIIQNNLK

YIGLC
Length:355
Mass (Da):40,850
Last modified:January 23, 2007 - v4
Checksum:i24590AE5B1FE5D50
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti210 – 2101K → R in AAC37651 (PubMed:8013366).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC014702 mRNA. Translation: AAH14702.1.
AF056973 Genomic DNA. Translation: AAC13569.1.
AF056972 mRNA. Translation: AAC13568.1.
L17074 mRNA. Translation: AAC37651.1.
CCDSiCCDS23922.1.
RefSeqiNP_034441.1. NM_010311.3.
XP_006513298.1. XM_006513235.1.
XP_006513299.1. XM_006513236.1.
XP_006513300.1. XM_006513237.1.
UniGeneiMm.32595.
Mm.393508.

Genome annotation databases

EnsembliENSMUST00000037813; ENSMUSP00000036087; ENSMUSG00000040009.
ENSMUST00000159991; ENSMUSP00000124639; ENSMUSG00000040009.
GeneIDi14687.
KEGGimmu:14687.
UCSCiuc007fpt.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC014702 mRNA. Translation: AAH14702.1.
AF056973 Genomic DNA. Translation: AAC13569.1.
AF056972 mRNA. Translation: AAC13568.1.
L17074 mRNA. Translation: AAC37651.1.
CCDSiCCDS23922.1.
RefSeqiNP_034441.1. NM_010311.3.
XP_006513298.1. XM_006513235.1.
XP_006513299.1. XM_006513236.1.
XP_006513300.1. XM_006513237.1.
UniGeneiMm.32595.
Mm.393508.

3D structure databases

ProteinModelPortaliO70443.
SMRiO70443. Positions 6-349.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70443. 1 interaction.

PTM databases

PhosphoSiteiO70443.

Proteomic databases

MaxQBiO70443.
PaxDbiO70443.
PRIDEiO70443.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037813; ENSMUSP00000036087; ENSMUSG00000040009.
ENSMUST00000159991; ENSMUSP00000124639; ENSMUSG00000040009.
GeneIDi14687.
KEGGimmu:14687.
UCSCiuc007fpt.1. mouse.

Organism-specific databases

CTDi2781.
MGIiMGI:95780. Gnaz.

Phylogenomic databases

eggNOGiNOG282619.
HOGENOMiHOG000038730.
HOVERGENiHBG063184.
InParanoidiO70443.
KOiK04535.
OMAiYDLRLYE.
OrthoDBiEOG72C50B.
PhylomeDBiO70443.
TreeFamiTF300673.

Enzyme and pathway databases

ReactomeiREACT_205726. G-protein activation.
REACT_242871. G alpha (s) signalling events.
REACT_250376. G alpha (i) signalling events.
REACT_253342. PLC beta mediated events.
REACT_258013. G alpha (z) signalling events.

Miscellaneous databases

NextBioi286627.
PROiO70443.
SOURCEiSearch...

Gene expression databases

BgeeiO70443.
ExpressionAtlasiO70443. baseline and differential.
GenevestigatoriO70443.

Family and domain databases

Gene3Di1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProiIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR10218. PTHR10218. 1 hit.
PfamiPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSiPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTiSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMiSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Eye.
  2. "Partial genomic sequence of the mouse GTP binding protein Gz alpha."
    Matthaei K.I., Mellick A.M., Hendry I.A.
    Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE OF 1-353.
    Strain: C57BL/6.
  3. "Expression of cone transducin, Gz alpha, and other G-protein alpha-subunit messenger ribonucleic acids in pancreatic islets."
    Zigman J.M., Westermark G.T., LaMendola J., Steiner D.F.
    Endocrinology 135:31-37(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 207-267.
    Strain: BALB/c.
    Tissue: Pancreatic islet.

Entry informationi

Entry nameiGNAZ_MOUSE
AccessioniPrimary (citable) accession number: O70443
Secondary accession number(s): O70442, Q61637, Q91WM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: March 4, 2015
This is version 135 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.