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O70443 (GNAZ_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanine nucleotide-binding protein G(z) subunit alpha
Alternative name(s):
G(x) alpha chain
Gz-alpha
Gene names
Name:Gnaz
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length355 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems.

Subunit structure

G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.

Subcellular location

Membrane; Lipid-anchor.

Sequence similarities

Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Ontologies

Keywords
   Cellular componentMembrane
   LigandGTP-binding
Magnesium
Metal-binding
Nucleotide-binding
   Molecular functionTransducer
   PTMLipoprotein
Myristate
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processG-protein coupled receptor signaling pathway

Traceable author statement PubMed 2508088. Source: MGI

adenylate cyclase-inhibiting G-protein coupled receptor signaling pathway

Inferred from electronic annotation. Source: Ensembl

adenylate cyclase-modulating G-protein coupled receptor signaling pathway

Inferred from Biological aspect of Ancestor. Source: RefGenome

regulation of GTPase activity

Inferred from Biological aspect of Ancestor. Source: GOC

   Cellular_componentcytosol

Inferred from electronic annotation. Source: Ensembl

extrinsic component of cytoplasmic side of plasma membrane

Inferred from Biological aspect of Ancestor. Source: RefGenome

heterotrimeric G-protein complex

Inferred from Biological aspect of Ancestor. Source: RefGenome

membrane

Traceable author statement PubMed 3923359. Source: MGI

   Molecular_functionG-protein beta/gamma-subunit complex binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

G-protein coupled serotonin receptor binding

Inferred from Biological aspect of Ancestor. Source: RefGenome

GTP binding

Inferred from electronic annotation. Source: UniProtKB-KW

GTPase activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

guanyl-nucleotide exchange factor activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

signal transducer activity

Inferred from Biological aspect of Ancestor. Source: RefGenome

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Probable
Chain2 – 355354Guanine nucleotide-binding protein G(z) subunit alpha
PRO_0000203697

Regions

Nucleotide binding40 – 478GTP By similarity
Nucleotide binding176 – 1827GTP By similarity
Nucleotide binding201 – 2055GTP By similarity
Nucleotide binding270 – 2734GTP By similarity

Sites

Metal binding471Magnesium By similarity
Metal binding1821Magnesium By similarity
Binding site3271GTP; via amide nitrogen By similarity

Amino acid modifications

Lipidation21N-myristoyl glycine By similarity
Lipidation31S-palmitoyl cysteine By similarity

Experimental info

Sequence conflict2101K → R in AAC37651. Ref.3

Sequences

Sequence LengthMass (Da)Tools
O70443 [UniParc].

Last modified January 23, 2007. Version 4.
Checksum: 24590AE5B1FE5D50

FASTA35540,850
        10         20         30         40         50         60 
MGCRQSSEEK EAARRSRRID RHLRSESQRQ RREIKLLLLG TSNSGKSTIV KQMKIIHSGG 

        70         80         90        100        110        120 
FNLDACKEYK PLIIYNAIDS LTRIIRALAA LKIDFHNPDR AYDAVQLFAL TGPAESKGEI 

       130        140        150        160        170        180 
TPELLGVMRR LWADPGAQAC FGRSSEYHLE DNAAYYLNDL ERIAAPDYIP TVEDILRSRD 

       190        200        210        220        230        240 
MTTGIVENKF TFKELTFKMV DVGGQRSERK KWIHCFEGVT AIIFCVELSG YDLKLYEDNQ 

       250        260        270        280        290        300 
TSRMAESLRL FDSICNNNWF INTSLILFLN KKDLLAEKIR RIPLSVCFPE YKGQNTYEEA 

       310        320        330        340        350 
AVYIQRQFED LNRNKETKEI YSHFTCATDT SNIQFVFDAV TDVIIQNNLK YIGLC 

« Hide

References

« Hide 'large scale' references
[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Eye.
[2]"Partial genomic sequence of the mouse GTP binding protein Gz alpha."
Matthaei K.I., Mellick A.M., Hendry I.A.
Submitted (APR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE OF 1-353.
Strain: C57BL/6.
[3]"Expression of cone transducin, Gz alpha, and other G-protein alpha-subunit messenger ribonucleic acids in pancreatic islets."
Zigman J.M., Westermark G.T., LaMendola J., Steiner D.F.
Endocrinology 135:31-37(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 207-267.
Strain: BALB/c.
Tissue: Pancreatic islet.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC014702 mRNA. Translation: AAH14702.1.
AF056973 Genomic DNA. Translation: AAC13569.1.
AF056972 mRNA. Translation: AAC13568.1.
L17074 mRNA. Translation: AAC37651.1.
CCDSCCDS23922.1.
RefSeqNP_034441.1. NM_010311.3.
XP_006513298.1. XM_006513235.1.
XP_006513299.1. XM_006513236.1.
XP_006513300.1. XM_006513237.1.
UniGeneMm.32595.
Mm.393508.

3D structure databases

ProteinModelPortalO70443.
SMRO70443. Positions 6-349.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActO70443. 1 interaction.

PTM databases

PhosphoSiteO70443.

Proteomic databases

MaxQBO70443.
PaxDbO70443.
PRIDEO70443.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000037813; ENSMUSP00000036087; ENSMUSG00000040009.
ENSMUST00000159991; ENSMUSP00000124639; ENSMUSG00000040009.
GeneID14687.
KEGGmmu:14687.
UCSCuc007fpt.1. mouse.

Organism-specific databases

CTD2781.
MGIMGI:95780. Gnaz.

Phylogenomic databases

eggNOGNOG282619.
HOGENOMHOG000038730.
HOVERGENHBG063184.
InParanoidO70443.
KOK04535.
OMADYKGQNT.
OrthoDBEOG72C50B.
PhylomeDBO70443.
TreeFamTF300673.

Gene expression databases

ArrayExpressO70443.
BgeeO70443.
GenevestigatorO70443.

Family and domain databases

Gene3D1.10.400.10. 1 hit.
3.40.50.300. 2 hits.
InterProIPR001408. Gprotein_alpha_I.
IPR001019. Gprotein_alpha_su.
IPR011025. GproteinA_insert.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERPTHR10218. PTHR10218. 1 hit.
PfamPF00503. G-alpha. 1 hit.
[Graphical view]
PRINTSPR00318. GPROTEINA.
PR00441. GPROTEINAI.
SMARTSM00275. G_alpha. 1 hit.
[Graphical view]
SUPFAMSSF47895. SSF47895. 1 hit.
SSF52540. SSF52540. 2 hits.
ProtoNetSearch...

Other

NextBio286627.
PROO70443.
SOURCESearch...

Entry information

Entry nameGNAZ_MOUSE
AccessionPrimary (citable) accession number: O70443
Secondary accession number(s): O70442, Q61637, Q91WM4
Entry history
Integrated into UniProtKB/Swiss-Prot: December 1, 2000
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 128 of the entry and version 4 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot