Reviewed, UniProtKB/Swiss-Prot O70441 (SYN3_RAT)

Names and origin

Protein names

Recommended name:
    Synapsin-3
Alternative name(s):
    Synapsin III

Gene names

Name:

Syn3

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in the regulation of neurotransmitter release and synaptogenesis. Binds ATP with high affinity and ADP with a lower affinity. This is consistent with a catalytic role of the C-domain in which ADP would be dissociated by cellular ATP after bound ATP was hydrolyzed.
Subunit structure	Interacts with CAPON. Ref.2
Subcellular location	Cytoplasmic vesicle › secretory vesicle › synaptic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Peripheral membrane protein localized to the cytoplasmic surface of synaptic vesicles.
Tissue specificity	Expressed primarily in brain.
Miscellaneous	Regulated by calcium.
Sequence similarities	Belongs to the synapsin family.

Ontologies

Keywords
Cellular component	Cell junction Cytoplasmic vesicle Membrane Synapse
Ligand	ATP-binding Calcium Nucleotide-binding
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	neurotransmitter secretion Ref.1 Traceable author statement. Source: RGD
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Ref.1 Inferred from direct assay. Source: RGD calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW catalytic activity Inferred from electronic annotation. Source: InterPro protein binding Ref.2 Inferred from physical interaction. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 579	579	Synapsin-3		PRO_0000183026
Regions
	Region	1 – 28	28	A
	Region	28 – 90	63	B; linker
	Region	91 – 398	308	C; actin-binding and synaptic-vesicle binding
	Region	399 – 530	132	J; Pro-rich linker
	Region	531 – 579	49	E
Amino acid modifications
	Modified residue	461	1	Phosphoserine By similarity
	Modified residue	469	1	Phosphoserine By similarity
	Modified residue	483	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O70441-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: EA69F25ED14A4722	FASTA	579	63,349
	10 20 30 40 50 60 MNFLRRRLSD SSFVANLPNG YMPDLQRPES SSSSPASPAT ERRHPQPLAA SFSSPGSSLF 70 80 90 100 110 120 SSFSSAMKQT PQAPTGLMEP PTPVTPVVQR PRILLVIDDA HTDWSKYFHG KKVNGDIEIR 130 140 150 160 170 180 VEQAEFSELN LAAYVTGGCM VDMQVVRNGT KIVRSFKPDF ILVRQHAYSM ALAEDYRSLV 190 200 210 220 230 240 IGLQYGGLPA VNSLYSVYNF CSKPWVFSQL IKIFHSLGPE KFPLVEQTFF PNHKPMLTAP 250 260 270 280 290 300 NFPVVIKLGH AHAGMGKIKV ENQHDYQDIT SVVAMAKTYA TTEAFIDSKY DIRIQKIGSN 310 320 330 340 350 360 YKAYMRTSIS GNWKANTGSA MLEQVAMTER YRLWVDSCSE MFGGLDICAV KAVHSKNGRD 370 380 390 400 410 420 YIIEVMDSSM PLIGEHVEED KQLMADLVVS KMSQLLVPGA SVPSPLRPWG PQTKSAKSPG 430 440 450 460 470 480 QGQLGPLLGQ PQPRPPPQGG PRQAQSPQPP RSRSPSQQRL SPQGQQPVSP QSGSPQQQRS 490 500 510 520 530 540 PGSPQLSRAS GGSSPNQASK PTASLSSHTR PPVQGRSTSQ QGEEPQKTAS PHPHLNKSQS 550 560 570 LTNSLSTSDT SHRGTPSEDE AKAETIRNLR KSFASLFSD

References

[1]	"Synapsin III, a novel synapsin with an unusual regulation by Ca2+." Hosaka M., Suedhof T.C. J. Biol. Chem. 273:13371-13374(1998) [PubMed: 9593663] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Brain.
[2]	"Neuronal nitric-oxide synthase localization mediated by a ternary complex with synapsin and CAPON." Jaffrey S.R., Benfenati F., Snowman A.M., Czernik A.J., Snyder S.H. Proc. Natl. Acad. Sci. U.S.A. 99:3199-3204(2002) [PubMed: 11867766] [Abstract] Cited for: INTERACTION WITH CAPON.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF056704 mRNA. Translation: AAC24521.1.
IPI	IPI00390215.
RefSeq	NP_058805.1.
UniGene	Rn.162920
3D structure databases
SMR	O70441. Positions 90-395.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-358N.
STRING	O70441.
PTM databases
PhosphoSite	O70441.
Genome annotation databases
Ensembl	ENSRNOT00000037918; ENSRNOP00000036740; ENSRNOG00000026866; Rattus norvegicus. [Genome view]
GeneID	29130.
KEGG	rno:29130.
UCSC	NM_017109. rat.
Organism-specific databases
CTD	29130.
RGD	3799. Syn3.
Phylogenomic databases
eggNOG	roNOG13960.
HOVERGEN	O70441.
InParanoid	O70441.
OMA	MQVVRNG.
OrthoDB	EOG97DD1S.
PhylomeDB	O70441.
Gene expression databases
ArrayExpress	O70441.
Genevestigator	O70441.
Family and domain databases
InterPro	IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp-like. IPR001359. Synapsin. IPR019735. Synapsin_CS. IPR019569. Synapsin_N. IPR019736. Synapsin_P_site. [Graphical view]
Gene3D	G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit.
Pfam	PF10581. Synapsin_N. 1 hit. [Graphical view]
PRINTS	PR01368. SYNAPSIN.
PROSITE	PS00415. SYNAPSIN_1. 1 hit. PS00416. SYNAPSIN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	608075.

Entry information

Entry name

SYN3_RAT

Accession

Primary (citable) accession number: O70441

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	February 9, 2010
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)