O70439 (STX7_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-7

Gene names

Name:	Stx7
Synonyms:	Syn7

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	261 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May be involved in protein trafficking from the plasma membrane to the early endosome (EE) as well as in homotypic fusion of endocytic organelles. Mediates the endocytic trafficking from early endosomes to late endosomes and lysosomes By similarity.
Subunit structure	Part of the SNARE core complex containing VAMP8, STX8 and VTI1B. Found in a complex with VAMP8 and VTI1B in the liver. Forms a SNARE complex with VTI1B, STX8 and VAMP8 which functions in the homotypic fusion of late endosomes. Interacts with VPS11, VPS16, VPS18 and VPS33A By similarity.
Subcellular location	Early endosome membrane; Single-pass type IV membrane protein By similarity.
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Endosome Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
Ligand	Iron
PTM	Acetylation Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	intracellular protein transport Inferred from electronic annotation. Source: InterPro vesicle-mediated transport Inferred from electronic annotation. Source: InterPro
Cellular component	early endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	SNAP receptor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 261

260

Syntaxin-7

PRO_0000210214

Regions

Topological domain

2 – 238

237

Cytoplasmic Potential

Transmembrane

239 – 259

21

Helical; Anchor for type IV membrane protein; Potential

Topological domain

260 – 261

2

Vesicular Potential

Domain

165 – 227

63

t-SNARE coiled-coil homology

Coiled coil

47 – 68

22

Potential

Amino acid modifications

Modified residue

2

1

N-acetylserine By similarity

Modified residue

3

1

Phosphotyrosine By similarity

Modified residue

45

1

Phosphoserine Ref.6

Modified residue

79

1

Phosphothreonine Ref.3 Ref.4

Modified residue

125

1

Phosphoserine Ref.5

Modified residue

126

1

Phosphoserine Ref.5

Modified residue

129

1

Phosphoserine Ref.5 Ref.6

Modified residue

205

1

Phosphoserine Ref.6

Secondary structure

1

.

.

.

261

Helix Strand Turn

Details...

Helix

170 – 226

57

Sequences

	Sequence		Length	Mass (Da)
	O70439 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: 62F4E4B33F247ADB	FASTA	261	29,821
	10 20 30 40 50 60 MSYTPGIGGD SAQLAQRISS NIQKITQCSV EIQRTLNQLG TPQDSPELRQ LLQQKQQYTN 70 80 90 100 110 120 QLAKETDKYI KEFGSLPTTP SEQRQRKIQK DRLVAEFTTS LTNFQKAQRQ AAEREKEFVA 130 140 150 160 170 180 RVRASSRVSG GFPEDSSKEK NLVSWESQTQ PQVQVQDEEI TEDDLRLIHE RESSIRQLEA 190 200 210 220 230 240 DIMDINEIFK DLGMMIHEQG DMIDSIEANV ESAEVHVQQA NQQLSRAADY QRKSRKTLCI 250 260 IIFILVVRIV IICLIVWGLK G

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Tellam J., Piper R.C., Smith C., James D.E. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Adipocyte.
[2]	"A role of VAMP8/endobrevin in regulated exocytosis of pancreatic acinar cells." Wang C.-C., Ng C.P., Lu L., Atlashkin V., Zhang W., Seet L.-F., Hong W. Dev. Cell 7:359-371(2004) [PubMed: 15363411] [Abstract] Cited for: IDENTIFICATION IN A COMPLEX WITH VAMP8 AND VTI1B.
[3]	"Phosphoproteomic analysis of the developing mouse brain." Ballif B.A., Villen J., Beausoleil S.A., Schwartz D., Gygi S.P. Mol. Cell. Proteomics 3:1093-1101(2004) [PubMed: 15345747] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-79, MASS SPECTROMETRY. Tissue: Embryonic brain.
[4]	"Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations." Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M. Mol. Cell. Proteomics 6:283-293(2007) [PubMed: 17114649] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-79, MASS SPECTROMETRY. Tissue: Brain cortex.
[5]	"Large-scale phosphorylation analysis of mouse liver." Villen J., Beausoleil S.A., Gerber S.A., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 104:1488-1493(2007) [PubMed: 17242355] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-125; SER-126 AND SER-129, MASS SPECTROMETRY. Tissue: Liver.
[6]	"The phagosomal proteome in interferon-gamma-activated macrophages." Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P. Immunity 30:143-154(2009) [PubMed: 19144319] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-45; SER-129 AND SER-205, MASS SPECTROMETRY. Tissue: Macrophage.
[7]	"Crystal structure of the endosomal SNARE complex reveals common structural principles of all SNAREs." Antonin W., Fasshauer D., Becker S., Jahn R., Schneider T.R. Nat. Struct. Biol. 9:107-111(2002) [PubMed: 11786915] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 169-229 IN COMPLEX WITH VAMP8; STX8 AND VTI1B.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF056323 mRNA. Translation: AAC15971.1.

IPI

IPI00118217.

UniGene

Mm.248042.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1GL2	X-ray	1.90	B	169-228	[»]

ProteinModelPortal

O70439.

SMR

O70439. Positions 2-259.

ModBase

Search...

Protein-protein interaction databases

IntAct

O70439. 3 interactions.

STRING

O70439.

PTM databases

PhosphoSite

O70439.

Proteomic databases

PRIDE

O70439.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Organism-specific databases

MGI

MGI:1858210. Stx7.

Phylogenomic databases

eggNOG

roNOG10770.

HOGENOM

HBG444570.

HOVERGEN

HBG053083.

InParanoid

O70439.

OrthoDB

EOG41NTN3.

PhylomeDB

O70439.

Gene expression databases

ArrayExpress

O70439.

Bgee

O70439.

CleanEx

MM_STX7.

Genevestigator

O70439.

GermOnline

ENSMUSG00000019998. Mus musculus.

Family and domain databases

InterPro

IPR006012. Syntaxin/epimorphin_CS.
IPR006011. Syntaxin_N.
IPR010989. t-SNARE.
IPR000727. T_SNARE_dom.
[Graphical view]

Pfam

PF05739. SNARE. 1 hit.
PF00804. Syntaxin. 1 hit.
[Graphical view]

SMART

SM00503. SynN. 1 hit.
SM00397. t_SNARE. 1 hit.
[Graphical view]

SUPFAM

SSF47661. t-snare. 1 hit.

PROSITE

PS00914. SYNTAXIN. 1 hit.
PS50192. T_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

SOURCE

Search...

Entry information

Entry name

STX7_MOUSE

Accession

Primary (citable) accession number: O70439

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 11, 2002
Last sequence update:	January 23, 2007
Last modified:	September 21, 2011
This is version 93 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program