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Protein

Four and a half LIM domains protein 2

Gene

Fhl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 3125C4-typeSequence AnalysisAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • atrial cardiac muscle cell development Source: BHF-UCL
  • heart trabecula formation Source: BHF-UCL
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: BHF-UCL
  • negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
  • osteoblast differentiation Source: BHF-UCL
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • response to hormone Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • ventricular cardiac muscle cell development Source: BHF-UCL
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

Metal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Four and a half LIM domains protein 2
Short name:
FHL-2
Alternative name(s):
Skeletal muscle LIM-protein 3
Short name:
SLIM-3
Gene namesi
Name:Fhl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 1

Organism-specific databases

MGIiMGI:1338762. Fhl2.

Subcellular locationi

GO - Cellular componenti

  • actin cytoskeleton Source: MGI
  • focal adhesion Source: MGI
  • M band Source: Ensembl
  • nucleoplasm Source: MGI
  • nucleus Source: BHF-UCL
  • Z disc Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Four and a half LIM domains protein 2PRO_0000075738Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei238 – 2381PhosphoserineBy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiO70433.
PRIDEiO70433.

PTM databases

PhosphoSiteiO70433.

Expressioni

Tissue specificityi

Highly expressed in heart but also detectable in brain and skeletal muscle.1 Publication

Gene expression databases

BgeeiO70433.
CleanExiMM_FHL2.
ExpressionAtlasiO70433. baseline and differential.
GenevisibleiO70433. MM.

Interactioni

Subunit structurei

Interacts with ZNF638 and TTN/titin. Interacts with E4F1. Interacts with GRB7. Interacts with SIRT1 and FOXO1 (By similarity).By similarity

Binary interactionsi

WithEntry#Exp.IntActNotes
Plcg2Q8CIH53EBI-299379,EBI-617954

Protein-protein interaction databases

BioGridi199669. 7 interactions.
IntActiO70433. 6 interactions.
MINTiMINT-152324.
STRINGi10090.ENSMUSP00000008280.

Structurei

3D structure databases

ProteinModelPortaliO70433.
SMRiO70433. Positions 1-159, 162-218, 225-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini40 – 9253LIM zinc-binding 1PROSITE-ProRule annotationAdd
BLAST
Domaini101 – 15353LIM zinc-binding 2PROSITE-ProRule annotationAdd
BLAST
Domaini162 – 21251LIM zinc-binding 3PROSITE-ProRule annotationAdd
BLAST
Domaini221 – 27555LIM zinc-binding 4PROSITE-ProRule annotationAdd
BLAST

Domaini

The third LIM zinc-binding mediates interaction with E4F1.By similarity

Sequence similaritiesi

Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri7 – 3125C4-typeSequence AnalysisAdd
BLAST

Keywords - Domaini

LIM domain, Repeat, Zinc-finger

Phylogenomic databases

eggNOGiNOG314122.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000231075.
HOVERGENiHBG074526.
InParanoidiO70433.
KOiK14380.
OMAiVACFEAL.
OrthoDBiEOG7P8P7M.
PhylomeDBiO70433.
TreeFamiTF321684.

Family and domain databases

Gene3Di2.10.110.10. 5 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70433-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTERFDCHHC NESLYGKKYI LKEENPHCVA CFEELYANTC EECGTPIGCD
60 70 80 90 100
CKDLSYKDRH WHEGCFHCSR CGSSLVDKPF AAKEEQLLCT DCYSNEYSSK
110 120 130 140 150
CQECKKTIMP GTRKMEYKGS SWHETCFTCQ RCQQPIGTKS FIPKENQNFC
160 170 180 190 200
VPCYEKQYAL QCVQCKKPIT TGGVTYREQP WHKECFVCTA CKKQLSGQRF
210 220 230 240 250
TARDEFPYCL TCFCDLYAKK CAGCTNPISG LGGTKYISFE ERQWHNDCFN
260 270
CKKCSLSLVG RGFLTERDDI LCPDCGKDI
Length:279
Mass (Da):32,073
Last modified:August 1, 1998 - v1
Checksum:i6D8CBC4B4424BFF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055889 mRNA. Translation: AAC12770.1.
U77040 mRNA. Translation: AAB19211.2.
AF114381 mRNA. Translation: AAD53230.1.
AF153340 mRNA. Translation: AAD34170.1.
CCDSiCCDS14922.1.
RefSeqiNP_001276462.1. NM_001289533.1.
NP_034342.1. NM_010212.4.
XP_011236736.1. XM_011238434.1.
UniGeneiMm.6799.

Genome annotation databases

EnsembliENSMUST00000008280; ENSMUSP00000008280; ENSMUSG00000008136.
ENSMUST00000185893; ENSMUSP00000141170; ENSMUSG00000008136.
GeneIDi14200.
KEGGimmu:14200.
UCSCiuc007avk.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF055889 mRNA. Translation: AAC12770.1.
U77040 mRNA. Translation: AAB19211.2.
AF114381 mRNA. Translation: AAD53230.1.
AF153340 mRNA. Translation: AAD34170.1.
CCDSiCCDS14922.1.
RefSeqiNP_001276462.1. NM_001289533.1.
NP_034342.1. NM_010212.4.
XP_011236736.1. XM_011238434.1.
UniGeneiMm.6799.

3D structure databases

ProteinModelPortaliO70433.
SMRiO70433. Positions 1-159, 162-218, 225-279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi199669. 7 interactions.
IntActiO70433. 6 interactions.
MINTiMINT-152324.
STRINGi10090.ENSMUSP00000008280.

PTM databases

PhosphoSiteiO70433.

Proteomic databases

PaxDbiO70433.
PRIDEiO70433.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000008280; ENSMUSP00000008280; ENSMUSG00000008136.
ENSMUST00000185893; ENSMUSP00000141170; ENSMUSG00000008136.
GeneIDi14200.
KEGGimmu:14200.
UCSCiuc007avk.2. mouse.

Organism-specific databases

CTDi2274.
MGIiMGI:1338762. Fhl2.

Phylogenomic databases

eggNOGiNOG314122.
GeneTreeiENSGT00760000118910.
HOGENOMiHOG000231075.
HOVERGENiHBG074526.
InParanoidiO70433.
KOiK14380.
OMAiVACFEAL.
OrthoDBiEOG7P8P7M.
PhylomeDBiO70433.
TreeFamiTF321684.

Miscellaneous databases

ChiTaRSiFhl2. mouse.
NextBioi285434.
PROiO70433.
SOURCEiSearch...

Gene expression databases

BgeeiO70433.
CleanExiMM_FHL2.
ExpressionAtlasiO70433. baseline and differential.
GenevisibleiO70433. MM.

Family and domain databases

Gene3Di2.10.110.10. 5 hits.
InterProiIPR001781. Znf_LIM.
[Graphical view]
PfamiPF00412. LIM. 4 hits.
[Graphical view]
SMARTiSM00132. LIM. 4 hits.
[Graphical view]
PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The cloning, sequencing and characterization of a mouse FHL2, which contains four and a half LIM domains."
    Chan K.K., Tsui S.K.W., Lee C.Y., Fung K.P., Waye M.M.Y.
    Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6J.
    Tissue: Brain.
  2. "The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle."
    Morgan M.J., Madgwick A.J.A.
    Biochem. Biophys. Res. Commun. 255:245-250(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Skeletal muscle.
  3. "Expression patterns of FHL/SLIM family members suggest important functional roles in skeletal muscle and cardiovascular system."
    Chu P.-H., Ruiz-Lozano P., Zhou Q., Cai C., Chen J.
    Mech. Dev. 95:259-265(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
  4. "Isolation of the mouse homolog mDRAL from skeletal muscle derived myoblasts."
    Starzinski-Powitz A., Martin B., Eckerdt F.
    Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.

Entry informationi

Entry nameiFHL2_MOUSE
AccessioniPrimary (citable) accession number: O70433
Secondary accession number(s): P97448
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: July 22, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.