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O70433

- FHL2_MOUSE

UniProt

O70433 - FHL2_MOUSE

Protein

Four and a half LIM domains protein 2

Gene

Fhl2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 114 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation By similarity.By similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri7 – 3125C4-typeSequence AnalysisAdd
    BLAST

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. transcription factor binding Source: UniProtKB
    3. zinc ion binding Source: InterPro

    GO - Biological processi

    1. atrial cardiac muscle cell development Source: BHF-UCL
    2. heart trabecula formation Source: BHF-UCL
    3. negative regulation of apoptotic process Source: UniProtKB
    4. negative regulation of transcription, DNA-templated Source: BHF-UCL
    5. negative regulation of transcription from RNA polymerase II promoter Source: UniProtKB
    6. osteoblast differentiation Source: BHF-UCL
    7. regulation of transcription from RNA polymerase II promoter Source: MGI
    8. response to hormone Source: Ensembl
    9. transcription, DNA-templated Source: UniProtKB-KW
    10. ventricular cardiac muscle cell development Source: BHF-UCL

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    ReactomeiREACT_198602. PPARA activates gene expression.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Four and a half LIM domains protein 2
    Short name:
    FHL-2
    Alternative name(s):
    Skeletal muscle LIM-protein 3
    Short name:
    SLIM-3
    Gene namesi
    Name:Fhl2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:1338762. Fhl2.

    Subcellular locationi

    Cytoplasm By similarity. Nucleus By similarity

    GO - Cellular componenti

    1. actin cytoskeleton Source: Ensembl
    2. focal adhesion Source: Ensembl
    3. M band Source: Ensembl
    4. nucleus Source: BHF-UCL
    5. Z disc Source: Ensembl

    Keywords - Cellular componenti

    Cytoplasm, Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 279279Four and a half LIM domains protein 2PRO_0000075738Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei238 – 2381PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiO70433.
    PaxDbiO70433.
    PRIDEiO70433.

    PTM databases

    PhosphoSiteiO70433.

    Expressioni

    Tissue specificityi

    Highly expressed in heart but also detectable in brain and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiO70433.
    BgeeiO70433.
    CleanExiMM_FHL2.
    GenevestigatoriO70433.

    Interactioni

    Subunit structurei

    Interacts with ZNF638 and TTN/titin. Interacts with E4F1. Interacts with GRB7. Interacts with SIRT1 and FOXO1 By similarity.By similarity

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    Plcg2Q8CIH53EBI-299379,EBI-617954

    Protein-protein interaction databases

    BioGridi199669. 7 interactions.
    IntActiO70433. 6 interactions.
    MINTiMINT-152324.

    Structurei

    3D structure databases

    ProteinModelPortaliO70433.
    SMRiO70433. Positions 1-159, 162-218, 225-279.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini40 – 9253LIM zinc-binding 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini101 – 15353LIM zinc-binding 2PROSITE-ProRule annotationAdd
    BLAST
    Domaini162 – 21251LIM zinc-binding 3PROSITE-ProRule annotationAdd
    BLAST
    Domaini221 – 27555LIM zinc-binding 4PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The third LIM zinc-binding mediates interaction with E4F1.By similarity

    Sequence similaritiesi

    Contains 4 LIM zinc-binding domains.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri7 – 3125C4-typeSequence AnalysisAdd
    BLAST

    Keywords - Domaini

    LIM domain, Repeat, Zinc-finger

    Phylogenomic databases

    eggNOGiNOG314122.
    HOGENOMiHOG000231075.
    HOVERGENiHBG074526.
    InParanoidiO70433.
    KOiK14380.
    OMAiYCVACFE.
    OrthoDBiEOG7P8P7M.
    PhylomeDBiO70433.
    TreeFamiTF321684.

    Family and domain databases

    Gene3Di2.10.110.10. 5 hits.
    InterProiIPR001781. Znf_LIM.
    [Graphical view]
    PfamiPF00412. LIM. 4 hits.
    [Graphical view]
    SMARTiSM00132. LIM. 4 hits.
    [Graphical view]
    PROSITEiPS00478. LIM_DOMAIN_1. 4 hits.
    PS50023. LIM_DOMAIN_2. 4 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    O70433-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTERFDCHHC NESLYGKKYI LKEENPHCVA CFEELYANTC EECGTPIGCD    50
    CKDLSYKDRH WHEGCFHCSR CGSSLVDKPF AAKEEQLLCT DCYSNEYSSK 100
    CQECKKTIMP GTRKMEYKGS SWHETCFTCQ RCQQPIGTKS FIPKENQNFC 150
    VPCYEKQYAL QCVQCKKPIT TGGVTYREQP WHKECFVCTA CKKQLSGQRF 200
    TARDEFPYCL TCFCDLYAKK CAGCTNPISG LGGTKYISFE ERQWHNDCFN 250
    CKKCSLSLVG RGFLTERDDI LCPDCGKDI 279
    Length:279
    Mass (Da):32,073
    Last modified:August 1, 1998 - v1
    Checksum:i6D8CBC4B4424BFF2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF055889 mRNA. Translation: AAC12770.1.
    U77040 mRNA. Translation: AAB19211.2.
    AF114381 mRNA. Translation: AAD53230.1.
    AF153340 mRNA. Translation: AAD34170.1.
    CCDSiCCDS14922.1.
    RefSeqiNP_001276462.1. NM_001289533.1.
    NP_034342.1. NM_010212.4.
    UniGeneiMm.6799.

    Genome annotation databases

    EnsembliENSMUST00000008280; ENSMUSP00000008280; ENSMUSG00000008136.
    GeneIDi14200.
    KEGGimmu:14200.
    UCSCiuc007avk.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF055889 mRNA. Translation: AAC12770.1 .
    U77040 mRNA. Translation: AAB19211.2 .
    AF114381 mRNA. Translation: AAD53230.1 .
    AF153340 mRNA. Translation: AAD34170.1 .
    CCDSi CCDS14922.1.
    RefSeqi NP_001276462.1. NM_001289533.1.
    NP_034342.1. NM_010212.4.
    UniGenei Mm.6799.

    3D structure databases

    ProteinModelPortali O70433.
    SMRi O70433. Positions 1-159, 162-218, 225-279.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 199669. 7 interactions.
    IntActi O70433. 6 interactions.
    MINTi MINT-152324.

    PTM databases

    PhosphoSitei O70433.

    Proteomic databases

    MaxQBi O70433.
    PaxDbi O70433.
    PRIDEi O70433.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000008280 ; ENSMUSP00000008280 ; ENSMUSG00000008136 .
    GeneIDi 14200.
    KEGGi mmu:14200.
    UCSCi uc007avk.1. mouse.

    Organism-specific databases

    CTDi 2274.
    MGIi MGI:1338762. Fhl2.

    Phylogenomic databases

    eggNOGi NOG314122.
    HOGENOMi HOG000231075.
    HOVERGENi HBG074526.
    InParanoidi O70433.
    KOi K14380.
    OMAi YCVACFE.
    OrthoDBi EOG7P8P7M.
    PhylomeDBi O70433.
    TreeFami TF321684.

    Enzyme and pathway databases

    Reactomei REACT_198602. PPARA activates gene expression.

    Miscellaneous databases

    ChiTaRSi FHL2. mouse.
    NextBioi 285434.
    PROi O70433.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O70433.
    Bgeei O70433.
    CleanExi MM_FHL2.
    Genevestigatori O70433.

    Family and domain databases

    Gene3Di 2.10.110.10. 5 hits.
    InterProi IPR001781. Znf_LIM.
    [Graphical view ]
    Pfami PF00412. LIM. 4 hits.
    [Graphical view ]
    SMARTi SM00132. LIM. 4 hits.
    [Graphical view ]
    PROSITEi PS00478. LIM_DOMAIN_1. 4 hits.
    PS50023. LIM_DOMAIN_2. 4 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The cloning, sequencing and characterization of a mouse FHL2, which contains four and a half LIM domains."
      Chan K.K., Tsui S.K.W., Lee C.Y., Fung K.P., Waye M.M.Y.
      Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6J.
      Tissue: Brain.
    2. "The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle."
      Morgan M.J., Madgwick A.J.A.
      Biochem. Biophys. Res. Commun. 255:245-250(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Skeletal muscle.
    3. "Expression patterns of FHL/SLIM family members suggest important functional roles in skeletal muscle and cardiovascular system."
      Chu P.-H., Ruiz-Lozano P., Zhou Q., Cai C., Chen J.
      Mech. Dev. 95:259-265(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    4. "Isolation of the mouse homolog mDRAL from skeletal muscle derived myoblasts."
      Starzinski-Powitz A., Martin B., Eckerdt F.
      Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.

    Entry informationi

    Entry nameiFHL2_MOUSE
    AccessioniPrimary (citable) accession number: O70433
    Secondary accession number(s): P97448
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: May 30, 2000
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 114 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3