O70433 (FHL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Four and a half LIM domains protein 2
Short name=FHL-2

Alternative name(s):
Skeletal muscle LIM-protein 3
Short name=SLIM-3

Gene names

Name:

Fhl2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	279 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation By similarity.
Subunit structure	Interacts with ZNF638 and TTN/titin. Interacts with E4F1. Interacts with GRB7. Interacts with SIRT1 and FOXO1 By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Tissue specificity	Highly expressed in heart but also detectable in brain and skeletal muscle. Ref.3
Domain	The third LIM zinc-binding mediates interaction with E4F1 By similarity.
Sequence similarities	Contains 4 LIM zinc-binding domains.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Cytoplasm Nucleus
Domain	LIM domain Repeat Zinc-finger
Ligand	Metal-binding Zinc
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	atrial cardiac muscle cell development Inferred from expression pattern. Source: BHF-UCL heart trabecula formation Inferred from expression pattern. Source: BHF-UCL negative regulation of apoptotic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription from RNA polymerase II promoter Inferred from sequence or structural similarity. Source: UniProtKB osteoblast differentiation Inferred from expression pattern. Source: BHF-UCL transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW ventricular cardiac muscle cell development Inferred from expression pattern. Source: BHF-UCL
Cellular component	nucleus Inferred from direct assay. Source: BHF-UCL
Molecular function	transcription factor binding Inferred from sequence or structural similarity. Source: UniProtKB zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Plcg2	Q8CIH5	3	EBI-299379,EBI-617954

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 279	279	Four and a half LIM domains protein 2		PRO_0000075738
Regions
	Domain	40 – 92	53	LIM zinc-binding 1
	Domain	101 – 153	53	LIM zinc-binding 2
	Domain	162 – 212	51	LIM zinc-binding 3
	Domain	221 – 275	55	LIM zinc-binding 4
	Zinc finger	7 – 31	25	C4-type Potential
Amino acid modifications
	Modified residue	238	1	Phosphoserine By similarity

Sequences

	Sequence		Length	Mass (Da)
	O70433 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 6D8CBC4B4424BFF2	FASTA	279	32,073
	10 20 30 40 50 60 MTERFDCHHC NESLYGKKYI LKEENPHCVA CFEELYANTC EECGTPIGCD CKDLSYKDRH 70 80 90 100 110 120 WHEGCFHCSR CGSSLVDKPF AAKEEQLLCT DCYSNEYSSK CQECKKTIMP GTRKMEYKGS 130 140 150 160 170 180 SWHETCFTCQ RCQQPIGTKS FIPKENQNFC VPCYEKQYAL QCVQCKKPIT TGGVTYREQP 190 200 210 220 230 240 WHKECFVCTA CKKQLSGQRF TARDEFPYCL TCFCDLYAKK CAGCTNPISG LGGTKYISFE 250 260 270 ERQWHNDCFN CKKCSLSLVG RGFLTERDDI LCPDCGKDI

References

[1]	"The cloning, sequencing and characterization of a mouse FHL2, which contains four and a half LIM domains." Chan K.K., Tsui S.K.W., Lee C.Y., Fung K.P., Waye M.M.Y. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6J. Tissue: Brain.
[2]	"The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle." Morgan M.J., Madgwick A.J.A. Biochem. Biophys. Res. Commun. 255:245-250(1999) [PubMed: 10049693] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Skeletal muscle.
[3]	"Expression patterns of FHL/SLIM family members suggest important functional roles in skeletal muscle and cardiovascular system." Chu P.-H., Ruiz-Lozano P., Zhou Q., Cai C., Chen J. Mech. Dev. 95:259-265(2000) [PubMed: 10906474] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[4]	"Isolation of the mouse homolog mDRAL from skeletal muscle derived myoblasts." Starzinski-Powitz A., Martin B., Eckerdt F. Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AF055889 mRNA. Translation: AAC12770.1. U77040 mRNA. Translation: AAB19211.2. AF114381 mRNA. Translation: AAD53230.1. AF153340 mRNA. Translation: AAD34170.1.
IPI	IPI00118205.
RefSeq	NP_034342.1. NM_010212.3.
UniGene	Mm.6799.
3D structure databases
ProteinModelPortal	O70433.
SMR	O70433. Positions 3-279.
ModBase	Search...
Protein-protein interaction databases
IntAct	O70433. 5 interactions.
MINT	MINT-152324.
STRING	O70433.
PTM databases
PhosphoSite	O70433.
Proteomic databases
PRIDE	O70433.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000008280; ENSMUSP00000008280; ENSMUSG00000008136.
GeneID	14200.
KEGG	mmu:14200.
Organism-specific databases
CTD	2274.
MGI	MGI:1338762. Fhl2.
Phylogenomic databases
eggNOG	roNOG08346.
HOGENOM	HBG443571.
HOVERGEN	HBG074526.
InParanoid	O70433.
OMA	KKPIGCD.
OrthoDB	EOG4XKV7B.
PhylomeDB	O70433.
Gene expression databases
ArrayExpress	O70433.
Bgee	O70433.
CleanEx	MM_FHL2.
Genevestigator	O70433.
GermOnline	ENSMUSG00000008136. Mus musculus.
Family and domain databases
InterPro	IPR001781. Znf_LIM. [Graphical view]
Gene3D	G3DSA:2.10.110.10. Znf_LIM. 5 hits.
KO	K14380.
Pfam	PF00412. LIM. 4 hits. [Graphical view]
SMART	SM00132. LIM. 4 hits. [Graphical view]
PROSITE	PS00478. LIM_DOMAIN_1. 4 hits. PS50023. LIM_DOMAIN_2. 4 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	285434.
SOURCE	Search...

Entry information

Entry name

FHL2_MOUSE

Accession

Primary (citable) accession number: O70433
Secondary accession number(s): P97448

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	January 25, 2012
This is version 92 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program