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O70433 (FHL2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Four and a half LIM domains protein 2

Short name=FHL-2
Alternative name(s):
Skeletal muscle LIM-protein 3
Short name=SLIM-3
Gene names
Name:Fhl2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May function as a molecular transmitter linking various signaling pathways to transcriptional regulation. Negatively regulates the transcriptional repressor E4F1 and may function in cell growth. Inhibits the transcriptional activity of FOXO1 and its apoptotic function by enhancing the interaction of FOXO1 with SIRT1 and FOXO1 deacetylation By similarity.

Subunit structure

Interacts with ZNF638 and TTN/titin. Interacts with E4F1. Interacts with GRB7. Interacts with SIRT1 and FOXO1 By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Tissue specificity

Highly expressed in heart but also detectable in brain and skeletal muscle. Ref.3

Domain

The third LIM zinc-binding mediates interaction with E4F1 By similarity.

Sequence similarities

Contains 4 LIM zinc-binding domains.

Ontologies

Keywords
   Biological processTranscription
Transcription regulation
   Cellular componentCytoplasm
Nucleus
   DomainLIM domain
Repeat
Zinc-finger
   LigandMetal-binding
Zinc
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processatrial cardiac muscle cell development

Inferred from expression pattern PubMed 15509787. Source: BHF-UCL

heart trabecula formation

Inferred from expression pattern PubMed 15509787. Source: BHF-UCL

negative regulation of apoptotic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription from RNA polymerase II promoter

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 15509787. Source: BHF-UCL

osteoblast differentiation

Inferred from expression pattern PubMed 16311053. Source: BHF-UCL

regulation of transcription from RNA polymerase II promoter

Inferred from direct assay PubMed 12151099. Source: MGI

response to hormone

Inferred from electronic annotation. Source: Ensembl

transcription, DNA-templated

Inferred from electronic annotation. Source: UniProtKB-KW

ventricular cardiac muscle cell development

Inferred from expression pattern PubMed 15509787. Source: BHF-UCL

   Cellular_componentM band

Inferred from electronic annotation. Source: Ensembl

Z disc

Inferred from electronic annotation. Source: Ensembl

actin cytoskeleton

Inferred from electronic annotation. Source: Ensembl

focal adhesion

Inferred from electronic annotation. Source: Ensembl

nucleus

Inferred from direct assay PubMed 15509787. Source: BHF-UCL

   Molecular_functionprotein binding

Inferred from physical interaction PubMed 15102471. Source: IntAct

transcription factor binding

Inferred from sequence or structural similarity. Source: UniProtKB

zinc ion binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Plcg2Q8CIH53EBI-299379,EBI-617954

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Four and a half LIM domains protein 2
PRO_0000075738

Regions

Domain40 – 9253LIM zinc-binding 1
Domain101 – 15353LIM zinc-binding 2
Domain162 – 21251LIM zinc-binding 3
Domain221 – 27555LIM zinc-binding 4
Zinc finger7 – 3125C4-type Potential

Amino acid modifications

Modified residue2381Phosphoserine By similarity

Sequences

Sequence LengthMass (Da)Tools
O70433 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 6D8CBC4B4424BFF2

FASTA27932,073
        10         20         30         40         50         60 
MTERFDCHHC NESLYGKKYI LKEENPHCVA CFEELYANTC EECGTPIGCD CKDLSYKDRH 

        70         80         90        100        110        120 
WHEGCFHCSR CGSSLVDKPF AAKEEQLLCT DCYSNEYSSK CQECKKTIMP GTRKMEYKGS 

       130        140        150        160        170        180 
SWHETCFTCQ RCQQPIGTKS FIPKENQNFC VPCYEKQYAL QCVQCKKPIT TGGVTYREQP 

       190        200        210        220        230        240 
WHKECFVCTA CKKQLSGQRF TARDEFPYCL TCFCDLYAKK CAGCTNPISG LGGTKYISFE 

       250        260        270 
ERQWHNDCFN CKKCSLSLVG RGFLTERDDI LCPDCGKDI 

« Hide

References

[1]"The cloning, sequencing and characterization of a mouse FHL2, which contains four and a half LIM domains."
Chan K.K., Tsui S.K.W., Lee C.Y., Fung K.P., Waye M.M.Y.
Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: C57BL/6J.
Tissue: Brain.
[2]"The LIM proteins FHL1 and FHL3 are expressed differently in skeletal muscle."
Morgan M.J., Madgwick A.J.A.
Biochem. Biophys. Res. Commun. 255:245-250(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Skeletal muscle.
[3]"Expression patterns of FHL/SLIM family members suggest important functional roles in skeletal muscle and cardiovascular system."
Chu P.-H., Ruiz-Lozano P., Zhou Q., Cai C., Chen J.
Mech. Dev. 95:259-265(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
[4]"Isolation of the mouse homolog mDRAL from skeletal muscle derived myoblasts."
Starzinski-Powitz A., Martin B., Eckerdt F.
Submitted (JUN-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF055889 mRNA. Translation: AAC12770.1.
U77040 mRNA. Translation: AAB19211.2.
AF114381 mRNA. Translation: AAD53230.1.
AF153340 mRNA. Translation: AAD34170.1.
CCDSCCDS14922.1.
RefSeqNP_001276462.1. NM_001289533.1.
NP_034342.1. NM_010212.4.
UniGeneMm.6799.

3D structure databases

ProteinModelPortalO70433.
SMRO70433. Positions 1-159, 162-218, 225-279.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid199669. 7 interactions.
IntActO70433. 6 interactions.
MINTMINT-152324.

PTM databases

PhosphoSiteO70433.

Proteomic databases

MaxQBO70433.
PaxDbO70433.
PRIDEO70433.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000008280; ENSMUSP00000008280; ENSMUSG00000008136.
GeneID14200.
KEGGmmu:14200.
UCSCuc007avk.1. mouse.

Organism-specific databases

CTD2274.
MGIMGI:1338762. Fhl2.

Phylogenomic databases

eggNOGNOG314122.
HOGENOMHOG000231075.
HOVERGENHBG074526.
InParanoidO70433.
KOK14380.
OMAYCVACFE.
OrthoDBEOG7P8P7M.
PhylomeDBO70433.
TreeFamTF321684.

Gene expression databases

ArrayExpressO70433.
BgeeO70433.
CleanExMM_FHL2.
GenevestigatorO70433.

Family and domain databases

Gene3D2.10.110.10. 5 hits.
InterProIPR001781. Znf_LIM.
[Graphical view]
PfamPF00412. LIM. 4 hits.
[Graphical view]
SMARTSM00132. LIM. 4 hits.
[Graphical view]
PROSITEPS00478. LIM_DOMAIN_1. 4 hits.
PS50023. LIM_DOMAIN_2. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSFHL2. mouse.
NextBio285434.
PROO70433.
SOURCESearch...

Entry information

Entry nameFHL2_MOUSE
AccessionPrimary (citable) accession number: O70433
Secondary accession number(s): P97448
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: July 9, 2014
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot