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Protein

Membrane primary amine oxidase

Gene

Aoc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis (By similarity).By similarity

Catalytic activityi

RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.By similarity

Cofactori

Protein has several cofactor binding sites:
  • Cu cationBy similarityNote: Binds 1 copper ion per subunit.By similarity
  • Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity
  • L-topaquinoneBy similarityNote: Contains 1 topaquinone per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei386Proton acceptorBy similarity1
Active sitei471Schiff-base intermediate with substrate; via topaquinoneBy similarity1
Metal bindingi520Copper; via tele nitrogenBy similarity1
Metal bindingi522Copper; via tele nitrogenBy similarity1
Metal bindingi529Calcium 1By similarity1
Metal bindingi530Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi531Calcium 1By similarity1
Metal bindingi572Calcium 2By similarity1
Metal bindingi663Calcium 2; via carbonyl oxygenBy similarity1
Metal bindingi667Calcium 2By similarity1
Metal bindingi673Calcium 1By similarity1
Metal bindingi674Calcium 1; via carbonyl oxygenBy similarity1
Metal bindingi684Copper; via pros nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Cell adhesion

Keywords - Ligandi

Calcium, Copper, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-211945. Phase 1 - Functionalization of compounds.

Names & Taxonomyi

Protein namesi
Recommended name:
Membrane primary amine oxidase (EC:1.4.3.21By similarity)
Alternative name(s):
Copper amine oxidase
Semicarbazide-sensitive amine oxidase
Short name:
SSAO
Vascular adhesion protein 1
Short name:
VAP-1
Gene namesi
Name:Aoc3
Synonyms:Vap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1306797. Aoc3.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 6CytoplasmicSequence analysis5
Transmembranei7 – 27Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini28 – 765ExtracellularSequence analysisAdd BLAST738

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4727.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000641032 – 765Membrane primary amine oxidaseAdd BLAST764

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi137N-linked (GlcNAc...)By similarity1
Disulfide bondi198 ↔ 199By similarity
Glycosylationi212O-linked (GalNAc...)By similarity1
Glycosylationi232N-linked (GlcNAc...)By similarity1
Glycosylationi294N-linked (GlcNAc...)By similarity1
Disulfide bondi404 ↔ 430By similarity
Modified residuei4712',4',5'-topaquinoneBy similarity1
Glycosylationi592N-linked (GlcNAc...) (complex)By similarity1
Glycosylationi659N-linked (GlcNAc...)Sequence analysis1
Glycosylationi666N-linked (GlcNAc...)By similarity1
Disulfide bondi734 ↔ 741By similarity
Disulfide bondi748InterchainBy similarity

Post-translational modificationi

N- and O-glycosylated.By similarity
Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, TPQ

Proteomic databases

MaxQBiO70423.
PaxDbiO70423.
PeptideAtlasiO70423.
PRIDEiO70423.

PTM databases

iPTMnetiO70423.
PhosphoSitePlusiO70423.

Expressioni

Gene expression databases

BgeeiENSMUSG00000019326.
CleanExiMM_AOC3.
ExpressionAtlasiO70423. baseline and differential.
GenevisibleiO70423. MM.

Interactioni

Subunit structurei

Homodimer; disulfide-linked (By similarity). Forms a heterodimer with AOC2 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198116. 1 interactor.
IntActiO70423. 4 interactors.
MINTiMINT-4087892.
STRINGi10090.ENSMUSP00000099394.

Chemistry databases

BindingDBiO70423.

Structurei

3D structure databases

ProteinModelPortaliO70423.
SMRiO70423.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni384 – 394Substrate bindingBy similarityAdd BLAST11
Regioni468 – 473Substrate bindingBy similarity6
Regioni578 – 585Heparin-bindingBy similarity8

Sequence similaritiesi

Belongs to the copper/topaquinone oxidase family.Curated

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1186. Eukaryota.
COG3733. LUCA.
GeneTreeiENSGT00510000046461.
HOGENOMiHOG000233919.
HOVERGENiHBG004164.
InParanoidiO70423.
KOiK00276.
OMAiDEGRFNW.
OrthoDBiEOG091G02WY.
PhylomeDBiO70423.
TreeFamiTF314750.

Family and domain databases

Gene3Di2.70.98.20. 1 hit.
InterProiIPR000269. Cu_amine_oxidase.
IPR015798. Cu_amine_oxidase_C.
IPR016182. Cu_amine_oxidase_N-reg.
IPR015800. Cu_amine_oxidase_N2.
IPR015802. Cu_amine_oxidase_N3.
[Graphical view]
PANTHERiPTHR10638. PTHR10638. 1 hit.
PfamiPF01179. Cu_amine_oxid. 1 hit.
PF02727. Cu_amine_oxidN2. 1 hit.
PF02728. Cu_amine_oxidN3. 1 hit.
[Graphical view]
PRINTSiPR00766. CUDAOXIDASE.
SUPFAMiSSF49998. SSF49998. 1 hit.
SSF54416. SSF54416. 2 hits.
PROSITEiPS01164. COPPER_AMINE_OXID_1. 1 hit.
PS01165. COPPER_AMINE_OXID_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O70423-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTQKTTLVLL ALAVITIFAL VCVLLAGRSG DGGGLSQPLH CPSVLPSVQP
60 70 80 90 100
RTHPSQSQPF ADLSPEELTA VMSFLTKHLG PGLVDAAQAR PSDNCVFSVE
110 120 130 140 150
LQLPAKAAAL AHLDRGGPPP VREALAIIFF GGQPKPNVSE LVVGPLPHPS
160 170 180 190 200
YMRDVTVERH GGPLPYYRRP VLDREYQDIE EMIFHRELPQ ASGLLHHCCF
210 220 230 240 250
YKHQGQNLLT MTTAPRGLQS GDRATWFGLY YNLSGAGFYP HPIGLELLID
260 270 280 290 300
HKALDPALWT IQKVFYQGRY YESLTQLEDQ FEAGLVNVVL VPNNGTGGSW
310 320 330 340 350
SLKSSVPPGP APPLQFHPQG PRFSVQGSQV SSSLWAFSFG LGAFSGPRIF
360 370 380 390 400
DIRFQGERVA YEISVQEAIA LYGGNSPASM STCYVDGSFG IGKYSTPLIR
410 420 430 440 450
GVDCPYLATY VDWHFLLESQ APKTLRDAFC VFEQNQGLPL RRHHSDFYSH
460 470 480 490 500
YFGGVVGTVL VVRSVSTLLN YDYIWDMVFH PNGAIEVKFH ATGYISSAFF
510 520 530 540 550
FGAGEKFGNR VGAHTLGTVH THSAHFKVDL DVAGLKNWAW AEDMAFVPTI
560 570 580 590 600
VPWQPEYQMQ RLQVTRKLLE TEEEAAFPLG GATPRYLYLA SNHSNKWGHR
610 620 630 640 650
RGYRIQILSF AGKPLPQESP IEKAFTWGRY HLAVTQRKEE EPSSSSIFNQ
660 670 680 690 700
NDPWTPTVNF TDFISNETIA GEDLVAWVTA GFLHIPHAED IPNTVTAGNS
710 720 730 740 750
VGFFLRPYNF FDEDPSFHSA DSIYFREGQD ATACEVNPLA CLSQTATCAP
760
EIPAFSHGGF AYRDN
Length:765
Mass (Da):84,534
Last modified:January 23, 2007 - v3
Checksum:i7489ED67D3DBB44D
GO
Isoform 2 (identifier: O70423-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     630-765: Missing.

Note: No experimental confirmation available.
Show »
Length:629
Mass (Da):69,527
Checksum:i96121FDE3D7473F4
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti659N → D in AAD09199 (PubMed:10092636).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_016604630 – 765Missing in isoform 2. 1 PublicationAdd BLAST136

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054831 mRNA. Translation: AAC23747.1.
AF078705 Genomic DNA. Translation: AAC35839.1.
AF115411 mRNA. Translation: AAD09199.1.
AL590969 Genomic DNA. Translation: CAM19551.1.
BC080857 mRNA. Translation: AAH80857.1.
CCDSiCCDS25465.1. [O70423-1]
RefSeqiNP_033805.1. NM_009675.2. [O70423-1]
UniGeneiMm.67281.

Genome annotation databases

EnsembliENSMUST00000103105; ENSMUSP00000099394; ENSMUSG00000019326. [O70423-1]
GeneIDi11754.
KEGGimmu:11754.
UCSCiuc007lop.2. mouse. [O70423-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054831 mRNA. Translation: AAC23747.1.
AF078705 Genomic DNA. Translation: AAC35839.1.
AF115411 mRNA. Translation: AAD09199.1.
AL590969 Genomic DNA. Translation: CAM19551.1.
BC080857 mRNA. Translation: AAH80857.1.
CCDSiCCDS25465.1. [O70423-1]
RefSeqiNP_033805.1. NM_009675.2. [O70423-1]
UniGeneiMm.67281.

3D structure databases

ProteinModelPortaliO70423.
SMRiO70423.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198116. 1 interactor.
IntActiO70423. 4 interactors.
MINTiMINT-4087892.
STRINGi10090.ENSMUSP00000099394.

Chemistry databases

BindingDBiO70423.
ChEMBLiCHEMBL4727.

PTM databases

iPTMnetiO70423.
PhosphoSitePlusiO70423.

Proteomic databases

MaxQBiO70423.
PaxDbiO70423.
PeptideAtlasiO70423.
PRIDEiO70423.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103105; ENSMUSP00000099394; ENSMUSG00000019326. [O70423-1]
GeneIDi11754.
KEGGimmu:11754.
UCSCiuc007lop.2. mouse. [O70423-1]

Organism-specific databases

CTDi8639.
MGIiMGI:1306797. Aoc3.

Phylogenomic databases

eggNOGiKOG1186. Eukaryota.
COG3733. LUCA.
GeneTreeiENSGT00510000046461.
HOGENOMiHOG000233919.
HOVERGENiHBG004164.
InParanoidiO70423.
KOiK00276.
OMAiDEGRFNW.
OrthoDBiEOG091G02WY.
PhylomeDBiO70423.
TreeFamiTF314750.

Enzyme and pathway databases

ReactomeiR-MMU-211945. Phase 1 - Functionalization of compounds.

Miscellaneous databases

PROiO70423.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000019326.
CleanExiMM_AOC3.
ExpressionAtlasiO70423. baseline and differential.
GenevisibleiO70423. MM.

Family and domain databases

Gene3Di2.70.98.20. 1 hit.
InterProiIPR000269. Cu_amine_oxidase.
IPR015798. Cu_amine_oxidase_C.
IPR016182. Cu_amine_oxidase_N-reg.
IPR015800. Cu_amine_oxidase_N2.
IPR015802. Cu_amine_oxidase_N3.
[Graphical view]
PANTHERiPTHR10638. PTHR10638. 1 hit.
PfamiPF01179. Cu_amine_oxid. 1 hit.
PF02727. Cu_amine_oxidN2. 1 hit.
PF02728. Cu_amine_oxidN3. 1 hit.
[Graphical view]
PRINTSiPR00766. CUDAOXIDASE.
SUPFAMiSSF49998. SSF49998. 1 hit.
SSF54416. SSF54416. 2 hits.
PROSITEiPS01164. COPPER_AMINE_OXID_1. 1 hit.
PS01165. COPPER_AMINE_OXID_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAOC3_MOUSE
AccessioniPrimary (citable) accession number: O70423
Secondary accession number(s): A2A4I7, Q66JM4, Q9R055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 145 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.