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O70423 (AOC3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Membrane primary amine oxidase

EC=1.4.3.21
Alternative name(s):
Copper amine oxidase
Semicarbazide-sensitive amine oxidase
Short name=SSAO
Vascular adhesion protein 1
Short name=VAP-1
Gene names
Name:Aoc3
Synonyms:Vap1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length765 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis By similarity.

Catalytic activity

RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.

Cofactor

Binds 1 copper ion per subunit By similarity.

Binds 2 calcium ions per subunit By similarity.

Contains 1 topaquinone per subunit By similarity.

Subunit structure

Homodimer; disulfide-linked. Forms a heterodimer with AOC2 By similarity.

Subcellular location

Membrane; Single-pass type II membrane protein By similarity.

Post-translational modification

Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.

N- and O-glycosylated By similarity.

Sequence similarities

Belongs to the copper/topaquinone oxidase family.

Ontologies

Keywords
   Biological processCell adhesion
   Cellular componentMembrane
   Coding sequence diversityAlternative splicing
   DomainSignal-anchor
Transmembrane
Transmembrane helix
   LigandCalcium
Copper
Metal-binding
   Molecular functionOxidoreductase
   PTMDisulfide bond
Glycoprotein
TPQ
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processamine metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

cell adhesion

Inferred from sequence or structural similarity. Source: UniProtKB

response to antibiotic

Inferred from electronic annotation. Source: Ensembl

   Cellular_componentcell surface

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from electronic annotation. Source: Ensembl

integral component of membrane

Inferred from sequence or structural similarity. Source: UniProtKB

microvillus

Inferred from electronic annotation. Source: Ensembl

plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionaliphatic-amine oxidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

aminoacetone:oxygen oxidoreductase(deaminating) activity

Inferred from electronic annotation. Source: UniProtKB-EC

calcium ion binding

Inferred from electronic annotation. Source: Ensembl

cation channel activity

Inferred from electronic annotation. Source: Ensembl

copper ion binding

Inferred from electronic annotation. Source: InterPro

phenethylamine:oxygen oxidoreductase (deaminating) activity

Inferred from electronic annotation. Source: UniProtKB-EC

primary amine oxidase activity

Inferred from sequence or structural similarity. Source: UniProtKB

quinone binding

Inferred from sequence or structural similarity. Source: UniProtKB

tryptamine:oxygen oxidoreductase (deaminating) activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 (identifier: O70423-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 (identifier: O70423-2)

The sequence of this isoform differs from the canonical sequence as follows:
     630-765: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 765764Membrane primary amine oxidase
PRO_0000064103

Regions

Topological domain2 – 65Cytoplasmic Potential
Transmembrane7 – 2721Helical; Signal-anchor for type II membrane protein; Potential
Topological domain28 – 765738Extracellular Potential

Sites

Active site3861Proton acceptor By similarity
Active site4711Schiff-base intermediate with substrate; via topaquinone By similarity
Metal binding5201Copper By similarity
Metal binding5221Copper By similarity
Metal binding5291Calcium 1 By similarity
Metal binding5301Calcium 1; via carbonyl oxygen By similarity
Metal binding5311Calcium 1 By similarity
Metal binding5721Calcium 2 By similarity
Metal binding6381Calcium 2 By similarity
Metal binding6631Calcium 2; via carbonyl oxygen By similarity
Metal binding6651Calcium 2 By similarity
Metal binding6671Calcium 2 By similarity
Metal binding6731Calcium 1 By similarity
Metal binding6741Calcium 1; via carbonyl oxygen By similarity
Metal binding6841Copper By similarity

Amino acid modifications

Modified residue47112',4',5'-topaquinone By similarity
Glycosylation1371N-linked (GlcNAc...) Potential
Glycosylation2121O-linked (GalNAc...) By similarity
Glycosylation2321N-linked (GlcNAc...) Potential
Glycosylation2941N-linked (GlcNAc...) Potential
Glycosylation5921N-linked (GlcNAc...) Potential
Glycosylation6591N-linked (GlcNAc...) Potential
Glycosylation6661N-linked (GlcNAc...) Potential
Disulfide bond198 ↔ 199 By similarity
Disulfide bond734 ↔ 741 By similarity
Disulfide bond748Interchain By similarity

Natural variations

Alternative sequence630 – 765136Missing in isoform 2.
VSP_016604

Experimental info

Sequence conflict6591N → D in AAD09199. Ref.2

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 7489ED67D3DBB44D

FASTA76584,534
        10         20         30         40         50         60 
MTQKTTLVLL ALAVITIFAL VCVLLAGRSG DGGGLSQPLH CPSVLPSVQP RTHPSQSQPF 

        70         80         90        100        110        120 
ADLSPEELTA VMSFLTKHLG PGLVDAAQAR PSDNCVFSVE LQLPAKAAAL AHLDRGGPPP 

       130        140        150        160        170        180 
VREALAIIFF GGQPKPNVSE LVVGPLPHPS YMRDVTVERH GGPLPYYRRP VLDREYQDIE 

       190        200        210        220        230        240 
EMIFHRELPQ ASGLLHHCCF YKHQGQNLLT MTTAPRGLQS GDRATWFGLY YNLSGAGFYP 

       250        260        270        280        290        300 
HPIGLELLID HKALDPALWT IQKVFYQGRY YESLTQLEDQ FEAGLVNVVL VPNNGTGGSW 

       310        320        330        340        350        360 
SLKSSVPPGP APPLQFHPQG PRFSVQGSQV SSSLWAFSFG LGAFSGPRIF DIRFQGERVA 

       370        380        390        400        410        420 
YEISVQEAIA LYGGNSPASM STCYVDGSFG IGKYSTPLIR GVDCPYLATY VDWHFLLESQ 

       430        440        450        460        470        480 
APKTLRDAFC VFEQNQGLPL RRHHSDFYSH YFGGVVGTVL VVRSVSTLLN YDYIWDMVFH 

       490        500        510        520        530        540 
PNGAIEVKFH ATGYISSAFF FGAGEKFGNR VGAHTLGTVH THSAHFKVDL DVAGLKNWAW 

       550        560        570        580        590        600 
AEDMAFVPTI VPWQPEYQMQ RLQVTRKLLE TEEEAAFPLG GATPRYLYLA SNHSNKWGHR 

       610        620        630        640        650        660 
RGYRIQILSF AGKPLPQESP IEKAFTWGRY HLAVTQRKEE EPSSSSIFNQ NDPWTPTVNF 

       670        680        690        700        710        720 
TDFISNETIA GEDLVAWVTA GFLHIPHAED IPNTVTAGNS VGFFLRPYNF FDEDPSFHSA 

       730        740        750        760 
DSIYFREGQD ATACEVNPLA CLSQTATCAP EIPAFSHGGF AYRDN 

« Hide

Isoform 2 [UniParc].

Checksum: 96121FDE3D7473F4
Show »

FASTA62969,527

References

« Hide 'large scale' references
[1]"Isolation, structural characterization, and chromosomal mapping of the mouse vascular adhesion protein-1 gene and promoter."
Bono P., Salmi M., Smith D.J., Leppanen I., Horelli-Kuitunen N., Palotie A., Jalkanen S.
J. Immunol. 161:2953-2960(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
Strain: 129/SvJ and BALB/c.
[2]"Molecular cloning of a major mRNA species in murine 3T3 adipocyte lineage. differentiation-dependent expression, regulation, and identification as semicarbazide-sensitive amine oxidase."
Moldes M., Feve B., Pairault J.
J. Biol. Chem. 274:9515-9523(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
Strain: C57BL/6.
Tissue: Brain.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF054831 mRNA. Translation: AAC23747.1.
AF078705 Genomic DNA. Translation: AAC35839.1.
AF115411 mRNA. Translation: AAD09199.1.
AL590969 Genomic DNA. Translation: CAM19551.1.
BC080857 mRNA. Translation: AAH80857.1.
CCDSCCDS25465.1. [O70423-1]
RefSeqNP_033805.1. NM_009675.2. [O70423-1]
UniGeneMm.67281.

3D structure databases

ProteinModelPortalO70423.
SMRO70423. Positions 58-761.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid198116. 1 interaction.
IntActO70423. 4 interactions.
MINTMINT-4087892.
STRING10090.ENSMUSP00000099394.

Chemistry

ChEMBLCHEMBL4727.

Proteomic databases

MaxQBO70423.
PaxDbO70423.
PRIDEO70423.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103105; ENSMUSP00000099394; ENSMUSG00000019326. [O70423-1]
GeneID11754.
KEGGmmu:11754.
UCSCuc007lop.2. mouse. [O70423-1]

Organism-specific databases

CTD8639.
MGIMGI:1306797. Aoc3.

Phylogenomic databases

eggNOGCOG3733.
GeneTreeENSGT00510000046461.
HOGENOMHOG000233919.
HOVERGENHBG004164.
InParanoidA2A4I7.
KOK00276.
OMASWERYQL.
OrthoDBEOG7353W8.
PhylomeDBO70423.
TreeFamTF314750.

Gene expression databases

ArrayExpressO70423.
BgeeO70423.
CleanExMM_AOC3.
GenevestigatorO70423.

Family and domain databases

Gene3D2.70.98.20. 1 hit.
3.10.450.40. 2 hits.
InterProIPR000269. Cu_amine_oxidase.
IPR015798. Cu_amine_oxidase_C.
IPR016182. Cu_amine_oxidase_N-reg.
IPR015800. Cu_amine_oxidase_N2.
IPR015801. Cu_amine_oxidase_N2/3.
IPR015802. Cu_amine_oxidase_N3.
[Graphical view]
PANTHERPTHR10638. PTHR10638. 1 hit.
PfamPF01179. Cu_amine_oxid. 1 hit.
PF02727. Cu_amine_oxidN2. 1 hit.
PF02728. Cu_amine_oxidN3. 1 hit.
[Graphical view]
PRINTSPR00766. CUDAOXIDASE.
SUPFAMSSF49998. SSF49998. 1 hit.
SSF54416. SSF54416. 2 hits.
PROSITEPS01164. COPPER_AMINE_OXID_1. 1 hit.
PS01165. COPPER_AMINE_OXID_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio279503.
PROO70423.
SOURCESearch...

Entry information

Entry nameAOC3_MOUSE
AccessionPrimary (citable) accession number: O70423
Secondary accession number(s): A2A4I7, Q66JM4, Q9R055
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 125 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot