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O70423

- AOC3_MOUSE

UniProt

O70423 - AOC3_MOUSE

Protein

Membrane primary amine oxidase

Gene

Aoc3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 126 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis By similarity.By similarity

    Catalytic activityi

    RCH2NH2 + H2O + O2 = RCHO + NH3 + H2O2.

    Cofactori

    Binds 1 copper ion per subunit.By similarity
    Binds 2 calcium ions per subunit.By similarity
    Contains 1 topaquinone per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei386 – 3861Proton acceptorBy similarity
    Active sitei471 – 4711Schiff-base intermediate with substrate; via topaquinoneBy similarity
    Metal bindingi520 – 5201CopperBy similarity
    Metal bindingi522 – 5221CopperBy similarity
    Metal bindingi529 – 5291Calcium 1By similarity
    Metal bindingi530 – 5301Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi531 – 5311Calcium 1By similarity
    Metal bindingi572 – 5721Calcium 2By similarity
    Metal bindingi638 – 6381Calcium 2By similarity
    Metal bindingi663 – 6631Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi665 – 6651Calcium 2By similarity
    Metal bindingi667 – 6671Calcium 2By similarity
    Metal bindingi673 – 6731Calcium 1By similarity
    Metal bindingi674 – 6741Calcium 1; via carbonyl oxygenBy similarity
    Metal bindingi684 – 6841CopperBy similarity

    GO - Molecular functioni

    1. aliphatic-amine oxidase activity Source: UniProtKB-EC
    2. aminoacetone:oxygen oxidoreductase(deaminating) activity Source: UniProtKB-EC
    3. calcium ion binding Source: Ensembl
    4. cation channel activity Source: Ensembl
    5. copper ion binding Source: InterPro
    6. phenethylamine:oxygen oxidoreductase (deaminating) activity Source: UniProtKB-EC
    7. primary amine oxidase activity Source: UniProtKB
    8. quinone binding Source: UniProtKB
    9. tryptamine:oxygen oxidoreductase (deaminating) activity Source: UniProtKB-EC

    GO - Biological processi

    1. amine metabolic process Source: UniProtKB
    2. cell adhesion Source: UniProtKB
    3. response to antibiotic Source: Ensembl

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Cell adhesion

    Keywords - Ligandi

    Calcium, Copper, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Membrane primary amine oxidase (EC:1.4.3.21)
    Alternative name(s):
    Copper amine oxidase
    Semicarbazide-sensitive amine oxidase
    Short name:
    SSAO
    Vascular adhesion protein 1
    Short name:
    VAP-1
    Gene namesi
    Name:Aoc3
    Synonyms:Vap1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 11

    Organism-specific databases

    MGIiMGI:1306797. Aoc3.

    Subcellular locationi

    Membrane By similarity; Single-pass type II membrane protein By similarity

    GO - Cellular componenti

    1. cell surface Source: UniProtKB
    2. cytoplasm Source: Ensembl
    3. integral component of membrane Source: UniProtKB
    4. microvillus Source: Ensembl
    5. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 765764Membrane primary amine oxidasePRO_0000064103Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi137 – 1371N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi198 ↔ 199By similarity
    Glycosylationi212 – 2121O-linked (GalNAc...)By similarity
    Glycosylationi232 – 2321N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi294 – 2941N-linked (GlcNAc...)Sequence Analysis
    Modified residuei471 – 47112',4',5'-topaquinoneBy similarity
    Glycosylationi592 – 5921N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi659 – 6591N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi666 – 6661N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi734 ↔ 741By similarity
    Disulfide bondi748 – 748InterchainBy similarity

    Post-translational modificationi

    Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
    N- and O-glycosylated.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, TPQ

    Proteomic databases

    MaxQBiO70423.
    PaxDbiO70423.
    PRIDEiO70423.

    Expressioni

    Gene expression databases

    ArrayExpressiO70423.
    BgeeiO70423.
    CleanExiMM_AOC3.
    GenevestigatoriO70423.

    Interactioni

    Subunit structurei

    Homodimer; disulfide-linked. Forms a heterodimer with AOC2 By similarity.By similarity

    Protein-protein interaction databases

    BioGridi198116. 1 interaction.
    IntActiO70423. 4 interactions.
    MINTiMINT-4087892.
    STRINGi10090.ENSMUSP00000099394.

    Structurei

    3D structure databases

    ProteinModelPortaliO70423.
    SMRiO70423. Positions 58-761.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 65CytoplasmicSequence Analysis
    Topological domaini28 – 765738ExtracellularSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the copper/topaquinone oxidase family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3733.
    GeneTreeiENSGT00510000046461.
    HOGENOMiHOG000233919.
    HOVERGENiHBG004164.
    InParanoidiA2A4I7.
    KOiK00276.
    OMAiSWERYQL.
    OrthoDBiEOG7353W8.
    PhylomeDBiO70423.
    TreeFamiTF314750.

    Family and domain databases

    Gene3Di2.70.98.20. 1 hit.
    3.10.450.40. 2 hits.
    InterProiIPR000269. Cu_amine_oxidase.
    IPR015798. Cu_amine_oxidase_C.
    IPR016182. Cu_amine_oxidase_N-reg.
    IPR015800. Cu_amine_oxidase_N2.
    IPR015801. Cu_amine_oxidase_N2/3.
    IPR015802. Cu_amine_oxidase_N3.
    [Graphical view]
    PANTHERiPTHR10638. PTHR10638. 1 hit.
    PfamiPF01179. Cu_amine_oxid. 1 hit.
    PF02727. Cu_amine_oxidN2. 1 hit.
    PF02728. Cu_amine_oxidN3. 1 hit.
    [Graphical view]
    PRINTSiPR00766. CUDAOXIDASE.
    SUPFAMiSSF49998. SSF49998. 1 hit.
    SSF54416. SSF54416. 2 hits.
    PROSITEiPS01164. COPPER_AMINE_OXID_1. 1 hit.
    PS01165. COPPER_AMINE_OXID_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: O70423-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MTQKTTLVLL ALAVITIFAL VCVLLAGRSG DGGGLSQPLH CPSVLPSVQP    50
    RTHPSQSQPF ADLSPEELTA VMSFLTKHLG PGLVDAAQAR PSDNCVFSVE 100
    LQLPAKAAAL AHLDRGGPPP VREALAIIFF GGQPKPNVSE LVVGPLPHPS 150
    YMRDVTVERH GGPLPYYRRP VLDREYQDIE EMIFHRELPQ ASGLLHHCCF 200
    YKHQGQNLLT MTTAPRGLQS GDRATWFGLY YNLSGAGFYP HPIGLELLID 250
    HKALDPALWT IQKVFYQGRY YESLTQLEDQ FEAGLVNVVL VPNNGTGGSW 300
    SLKSSVPPGP APPLQFHPQG PRFSVQGSQV SSSLWAFSFG LGAFSGPRIF 350
    DIRFQGERVA YEISVQEAIA LYGGNSPASM STCYVDGSFG IGKYSTPLIR 400
    GVDCPYLATY VDWHFLLESQ APKTLRDAFC VFEQNQGLPL RRHHSDFYSH 450
    YFGGVVGTVL VVRSVSTLLN YDYIWDMVFH PNGAIEVKFH ATGYISSAFF 500
    FGAGEKFGNR VGAHTLGTVH THSAHFKVDL DVAGLKNWAW AEDMAFVPTI 550
    VPWQPEYQMQ RLQVTRKLLE TEEEAAFPLG GATPRYLYLA SNHSNKWGHR 600
    RGYRIQILSF AGKPLPQESP IEKAFTWGRY HLAVTQRKEE EPSSSSIFNQ 650
    NDPWTPTVNF TDFISNETIA GEDLVAWVTA GFLHIPHAED IPNTVTAGNS 700
    VGFFLRPYNF FDEDPSFHSA DSIYFREGQD ATACEVNPLA CLSQTATCAP 750
    EIPAFSHGGF AYRDN 765
    Length:765
    Mass (Da):84,534
    Last modified:January 23, 2007 - v3
    Checksum:i7489ED67D3DBB44D
    GO
    Isoform 2 (identifier: O70423-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         630-765: Missing.

    Note: No experimental confirmation available.

    Show »
    Length:629
    Mass (Da):69,527
    Checksum:i96121FDE3D7473F4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti659 – 6591N → D in AAD09199. (PubMed:10092636)Curated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei630 – 765136Missing in isoform 2. 1 PublicationVSP_016604Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF054831 mRNA. Translation: AAC23747.1.
    AF078705 Genomic DNA. Translation: AAC35839.1.
    AF115411 mRNA. Translation: AAD09199.1.
    AL590969 Genomic DNA. Translation: CAM19551.1.
    BC080857 mRNA. Translation: AAH80857.1.
    CCDSiCCDS25465.1. [O70423-1]
    RefSeqiNP_033805.1. NM_009675.2. [O70423-1]
    UniGeneiMm.67281.

    Genome annotation databases

    EnsembliENSMUST00000103105; ENSMUSP00000099394; ENSMUSG00000019326. [O70423-1]
    GeneIDi11754.
    KEGGimmu:11754.
    UCSCiuc007lop.2. mouse. [O70423-1]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF054831 mRNA. Translation: AAC23747.1 .
    AF078705 Genomic DNA. Translation: AAC35839.1 .
    AF115411 mRNA. Translation: AAD09199.1 .
    AL590969 Genomic DNA. Translation: CAM19551.1 .
    BC080857 mRNA. Translation: AAH80857.1 .
    CCDSi CCDS25465.1. [O70423-1 ]
    RefSeqi NP_033805.1. NM_009675.2. [O70423-1 ]
    UniGenei Mm.67281.

    3D structure databases

    ProteinModelPortali O70423.
    SMRi O70423. Positions 58-761.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 198116. 1 interaction.
    IntActi O70423. 4 interactions.
    MINTi MINT-4087892.
    STRINGi 10090.ENSMUSP00000099394.

    Chemistry

    ChEMBLi CHEMBL4727.

    Proteomic databases

    MaxQBi O70423.
    PaxDbi O70423.
    PRIDEi O70423.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000103105 ; ENSMUSP00000099394 ; ENSMUSG00000019326 . [O70423-1 ]
    GeneIDi 11754.
    KEGGi mmu:11754.
    UCSCi uc007lop.2. mouse. [O70423-1 ]

    Organism-specific databases

    CTDi 8639.
    MGIi MGI:1306797. Aoc3.

    Phylogenomic databases

    eggNOGi COG3733.
    GeneTreei ENSGT00510000046461.
    HOGENOMi HOG000233919.
    HOVERGENi HBG004164.
    InParanoidi A2A4I7.
    KOi K00276.
    OMAi SWERYQL.
    OrthoDBi EOG7353W8.
    PhylomeDBi O70423.
    TreeFami TF314750.

    Miscellaneous databases

    NextBioi 279503.
    PROi O70423.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O70423.
    Bgeei O70423.
    CleanExi MM_AOC3.
    Genevestigatori O70423.

    Family and domain databases

    Gene3Di 2.70.98.20. 1 hit.
    3.10.450.40. 2 hits.
    InterProi IPR000269. Cu_amine_oxidase.
    IPR015798. Cu_amine_oxidase_C.
    IPR016182. Cu_amine_oxidase_N-reg.
    IPR015800. Cu_amine_oxidase_N2.
    IPR015801. Cu_amine_oxidase_N2/3.
    IPR015802. Cu_amine_oxidase_N3.
    [Graphical view ]
    PANTHERi PTHR10638. PTHR10638. 1 hit.
    Pfami PF01179. Cu_amine_oxid. 1 hit.
    PF02727. Cu_amine_oxidN2. 1 hit.
    PF02728. Cu_amine_oxidN3. 1 hit.
    [Graphical view ]
    PRINTSi PR00766. CUDAOXIDASE.
    SUPFAMi SSF49998. SSF49998. 1 hit.
    SSF54416. SSF54416. 2 hits.
    PROSITEi PS01164. COPPER_AMINE_OXID_1. 1 hit.
    PS01165. COPPER_AMINE_OXID_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation, structural characterization, and chromosomal mapping of the mouse vascular adhesion protein-1 gene and promoter."
      Bono P., Salmi M., Smith D.J., Leppanen I., Horelli-Kuitunen N., Palotie A., Jalkanen S.
      J. Immunol. 161:2953-2960(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORM 1).
      Strain: 129/SvJ and BALB/c.
    2. "Molecular cloning of a major mRNA species in murine 3T3 adipocyte lineage. differentiation-dependent expression, regulation, and identification as semicarbazide-sensitive amine oxidase."
      Moldes M., Feve B., Pairault J.
      J. Biol. Chem. 274:9515-9523(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiAOC3_MOUSE
    AccessioniPrimary (citable) accession number: O70423
    Secondary accession number(s): A2A4I7, Q66JM4, Q9R055
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 15, 1999
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 126 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3