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Protein

General transcription factor IIH subunit 4

Gene

Gtf2h4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module CAK controls the initiation of transcription.By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair, Transcription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-112382 Formation of RNA Pol II elongation complex
R-MMU-113418 Formation of the Early Elongation Complex
R-MMU-5696395 Formation of Incision Complex in GG-NER
R-MMU-5696400 Dual Incision in GG-NER
R-MMU-674695 RNA Polymerase II Pre-transcription Events
R-MMU-6781823 Formation of TC-NER Pre-Incision Complex
R-MMU-6782135 Dual incision in TC-NER
R-MMU-6782210 Gap-filling DNA repair synthesis and ligation in TC-NER
R-MMU-6796648 TP53 Regulates Transcription of DNA Repair Genes
R-MMU-72086 mRNA Capping
R-MMU-73762 RNA Polymerase I Transcription Initiation
R-MMU-73772 RNA Polymerase I Promoter Escape
R-MMU-73776 RNA Polymerase II Promoter Escape
R-MMU-73777 RNA Polymerase I Chain Elongation
R-MMU-73779 RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
R-MMU-73863 RNA Polymerase I Transcription Termination
R-MMU-75953 RNA Polymerase II Transcription Initiation
R-MMU-75955 RNA Polymerase II Transcription Elongation
R-MMU-76042 RNA Polymerase II Transcription Initiation And Promoter Clearance
R-MMU-77075 RNA Pol II CTD phosphorylation and interaction with CE

Names & Taxonomyi

Protein namesi
Recommended name:
General transcription factor IIH subunit 4
Alternative name(s):
Basic transcription factor 2 52 kDa subunit
Short name:
BTF2 p52
General transcription factor IIH polypeptide 4
TFIIH basal transcription factor complex p52 subunit
Gene namesi
Name:Gtf2h4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:1338799 Gtf2h4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001192541 – 463General transcription factor IIH subunit 4Add BLAST463

Proteomic databases

EPDiO70422
PaxDbiO70422
PeptideAtlasiO70422
PRIDEiO70422

PTM databases

iPTMnetiO70422
PhosphoSitePlusiO70422

Expressioni

Gene expression databases

BgeeiENSMUSG00000001524
CleanExiMM_GTF2H4
ExpressionAtlasiO70422 baseline and differential
GenevisibleiO70422 MM

Interactioni

Subunit structurei

Component of the 7-subunit TFIIH core complex composed of XPB/ERCC3, XPD/ERCC2, GTF2H1, GTF2H2, GTF2H3, GTF2H4 and GTF2H5, which is active in NER. The core complex associates with the 3-subunit CDK-activating kinase (CAK) module composed of CCNH/cyclin H, CDK7 and MNAT1 to form the 10-subunit holoenzyme (holo-TFIIH) active in transcription.By similarity

Protein-protein interaction databases

BioGridi2001121 interactor.
CORUMiO70422
IntActiO70422 2 interactors.
MINTiO70422
STRINGi10090.ENSMUSP00000001565

Structurei

3D structure databases

ProteinModelPortaliO70422
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TFB2 family.Curated

Phylogenomic databases

eggNOGiKOG3471 Eukaryota
COG5144 LUCA
GeneTreeiENSGT00390000014159
HOGENOMiHOG000170529
HOVERGENiHBG054205
InParanoidiO70422
KOiK03144
OMAiYLEQYAH
OrthoDBiEOG091G06YE
PhylomeDBiO70422
TreeFamiTF300879

Family and domain databases

InterProiView protein in InterPro
IPR004598 TFIIH_p52/Tfb2
PANTHERiPTHR13152 PTHR13152, 1 hit
PfamiView protein in Pfam
PF03849 Tfb2, 1 hit
TIGRFAMsiTIGR00625 tfb2, 1 hit

Sequencei

Sequence statusi: Complete.

O70422-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEITPARGGL NRAHLQCRNL QEFLGGLSPG VLDRLYGHPA TCLAVFRELP
60 70 80 90 100
SLAKNWVMRM LFLEQPLPQA AVALWVKKEF SKAQEESTGL LSGLRIWHTQ
110 120 130 140 150
LLPGGLQGLI LNPVFRQNLR IALLGGGKAW SDDTSQLGPD KHARDVPSLD
160 170 180 190 200
KYAEERWEVV LHFMVGSPSA AVSQDLAQLL SQAGLMKSTE PGEPPCITSA
210 220 230 240 250
GFQFLLLDTP AQLWYFMLQY LQTAQSRGMD LVEILSFLFQ LSFSTLGKDY
260 270 280 290 300
SVEGMSDSLL NFLQHLREFG LVFQRKRKSR RYYPTRLAIN LSSGVSGAGG
310 320 330 340 350
TVHQPGFIVV ETNYRLYAYT ESELQIALIA LFSEMLYRFP NMVVAQVTRE
360 370 380 390 400
SVQQAIASGI TAQQIIHFLR TRAHPVMLKQ NPVLPPTITD QIRLWELERD
410 420 430 440 450
RLRFTEGVLY NQFLSQVDFE LLLAHARELG VLVFENSAKR LMVVTPAGHS
460
DVKRFWKRQK HSS
Length:463
Mass (Da):52,224
Last modified:August 1, 1998 - v1
Checksum:iDBAA15CB388C5AFC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF054823 mRNA Translation: AAC08594.1
BC012638 mRNA Translation: AAH12638.1
CCDSiCCDS28702.1
RefSeqiNP_034494.1, NM_010364.4
XP_006523731.1, XM_006523668.3
XP_006523732.1, XM_006523669.3
UniGeneiMm.10182

Genome annotation databases

EnsembliENSMUST00000001565; ENSMUSP00000001565; ENSMUSG00000001524
ENSMUST00000160734; ENSMUSP00000124335; ENSMUSG00000001524
GeneIDi14885
KEGGimmu:14885
UCSCiuc008cig.2 mouse

Similar proteinsi

Entry informationi

Entry nameiTF2H4_MOUSE
AccessioniPrimary (citable) accession number: O70422
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: August 1, 1998
Last modified: March 28, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome