ID   I27RA_MOUSE             Reviewed;         623 AA.
AC   O70394; E9QNY4;
DT   29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   27-MAR-2024, entry version 165.
DE   RecName: Full=Interleukin-27 receptor subunit alpha;
DE            Short=IL-27 receptor subunit alpha;
DE            Short=IL-27R subunit alpha;
DE            Short=IL-27R-alpha;
DE            Short=IL-27RA;
DE   AltName: Full=Type I T-cell cytokine receptor;
DE            Short=TCCR;
DE   AltName: Full=WSX-1;
DE   Flags: Precursor;
GN   Name=Il27ra; Synonyms=Tccr, Wsx1;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Spleen;
RX   PubMed=9600072; DOI=10.1006/bbrc.1998.8576;
RA   Sprecher C.A., Grant F.J., Baumgartner J.W., Presnell S.R., Schrader S.K.,
RA   Yamagiwa T., Whitmore T.E., O'Hara P.J., Foster D.F.;
RT   "Cloning and characterization of a novel class I cytokine receptor.";
RL   Biochem. Biophys. Res. Commun. 246:82-90(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC   TISSUE=Spleen;
RX   PubMed=11057672; DOI=10.1038/35038103;
RA   Chen Q., Ghilardi N., Wang H., Baker T., Xie M.-H., Gurney A., Grewal I.S.,
RA   de Sauvage F.J.;
RT   "Development of Th1-type immune responses requires the type I cytokine
RT   receptor TCCR.";
RL   Nature 407:916-920(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Mammary gland;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- FUNCTION: Receptor for IL27. Requires IL6ST/GP130 to mediate signal
CC       transduction in response to IL27. This signaling system acts through
CC       STAT3 and STAT1. Involved in the regulation of Th1-type immune
CC       responses. Also appears to be involved in innate defense mechanisms.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC   -!- TISSUE SPECIFICITY: Expressed in CD4+ and CD8+ T-cells, B-cells,
CC       natural killer cells and macrophages. Highest levels in CD4+ T-cells
CC       and natural killer cells. Expression highest in Th0 cells.
CC       {ECO:0000269|PubMed:11057672}.
CC   -!- INDUCTION: Down-regulated on differentiation of CD4+ T-cells in both
CC       Th1 and TH2 cells.
CC   -!- DOMAIN: The WSXWS motif appears to be necessary for proper protein
CC       folding and thereby efficient intracellular transport and cell-surface
CC       receptor binding.
CC   -!- DOMAIN: The box 1 motif is required for JAK interaction and/or
CC       activation.
CC   -!- SIMILARITY: Belongs to the type I cytokine receptor family. Type 2
CC       subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF053005; AAC40121.1; -; mRNA.
DR   EMBL; AF265243; AAG27298.1; -; mRNA.
DR   EMBL; AC156028; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC032878; AAH32878.1; -; mRNA.
DR   CCDS; CCDS22465.1; -.
DR   RefSeq; NP_057880.2; NM_016671.3.
DR   PDB; 7Z0L; EM; 4.00 A; C=28-224.
DR   PDB; 7ZG0; X-ray; 3.18 A; E/F=24-225.
DR   PDBsum; 7Z0L; -.
DR   PDBsum; 7ZG0; -.
DR   AlphaFoldDB; O70394; -.
DR   EMDB; EMD-14427; -.
DR   SMR; O70394; -.
DR   STRING; 10090.ENSMUSP00000005601; -.
DR   GlyCosmos; O70394; 6 sites, No reported glycans.
DR   GlyGen; O70394; 6 sites.
DR   PhosphoSitePlus; O70394; -.
DR   SwissPalm; O70394; -.
DR   EPD; O70394; -.
DR   MaxQB; O70394; -.
DR   PaxDb; 10090-ENSMUSP00000005601; -.
DR   ProteomicsDB; 267033; -.
DR   Antibodypedia; 13590; 513 antibodies from 35 providers.
DR   DNASU; 50931; -.
DR   Ensembl; ENSMUST00000005601.9; ENSMUSP00000005601.8; ENSMUSG00000005465.10.
DR   GeneID; 50931; -.
DR   KEGG; mmu:50931; -.
DR   UCSC; uc009mlr.2; mouse.
DR   AGR; MGI:1355318; -.
DR   CTD; 9466; -.
DR   MGI; MGI:1355318; Il27ra.
DR   VEuPathDB; HostDB:ENSMUSG00000005465; -.
DR   eggNOG; ENOG502RF72; Eukaryota.
DR   GeneTree; ENSGT00700000104610; -.
DR   HOGENOM; CLU_029896_0_0_1; -.
DR   InParanoid; O70394; -.
DR   OMA; TCCCSLI; -.
DR   OrthoDB; 5323122at2759; -.
DR   PhylomeDB; O70394; -.
DR   TreeFam; TF338122; -.
DR   Reactome; R-MMU-8984722; Interleukin-35 Signalling.
DR   Reactome; R-MMU-9020956; Interleukin-27 signaling.
DR   BioGRID-ORCS; 50931; 4 hits in 114 CRISPR screens.
DR   ChiTaRS; Il27ra; mouse.
DR   PRO; PR:O70394; -.
DR   Proteomes; UP000000589; Chromosome 8.
DR   RNAct; O70394; Protein.
DR   Bgee; ENSMUSG00000005465; Expressed in ileal epithelium and 66 other cell types or tissues.
DR   GO; GO:0005737; C:cytoplasm; TAS:Reactome.
DR   GO; GO:0009897; C:external side of plasma membrane; IBA:GO_Central.
DR   GO; GO:0005576; C:extracellular region; TAS:Reactome.
DR   GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR   GO; GO:0043235; C:receptor complex; IBA:GO_Central.
DR   GO; GO:0019955; F:cytokine binding; IBA:GO_Central.
DR   GO; GO:0045509; F:interleukin-27 receptor activity; IDA:MGI.
DR   GO; GO:0019221; P:cytokine-mediated signaling pathway; IBA:GO_Central.
DR   GO; GO:0050830; P:defense response to Gram-positive bacterium; IMP:MGI.
DR   GO; GO:0002692; P:negative regulation of cellular extravasation; IGI:BHF-UCL.
DR   GO; GO:0032700; P:negative regulation of interleukin-17 production; IGI:BHF-UCL.
DR   GO; GO:0032715; P:negative regulation of interleukin-6 production; IGI:BHF-UCL.
DR   GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISO:MGI.
DR   GO; GO:2000408; P:negative regulation of T cell extravasation; IGI:BHF-UCL.
DR   GO; GO:2000317; P:negative regulation of T-helper 17 type immune response; IGI:BHF-UCL.
DR   GO; GO:0032720; P:negative regulation of tumor necrosis factor production; IGI:BHF-UCL.
DR   GO; GO:0002829; P:negative regulation of type 2 immune response; IMP:MGI.
DR   GO; GO:0002827; P:positive regulation of T-helper 1 type immune response; IMP:MGI.
DR   GO; GO:0032729; P:positive regulation of type II interferon production; IMP:MGI.
DR   GO; GO:0048302; P:regulation of isotype switching to IgG isotypes; IMP:MGI.
DR   CDD; cd00063; FN3; 1.
DR   Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR   InterPro; IPR003961; FN3_dom.
DR   InterPro; IPR036116; FN3_sf.
DR   InterPro; IPR013783; Ig-like_fold.
DR   PANTHER; PTHR48335; INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA; 1.
DR   PANTHER; PTHR48335:SF1; INTERLEUKIN-27 RECEPTOR SUBUNIT ALPHA; 1.
DR   SMART; SM00060; FN3; 3.
DR   SUPFAM; SSF49265; Fibronectin type III; 2.
DR   PROSITE; PS50853; FN3; 4.
DR   Genevisible; O70394; MM.
PE   1: Evidence at protein level;
KW   3D-structure; Glycoprotein; Membrane; Receptor; Reference proteome; Repeat;
KW   Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..623
FT                   /note="Interleukin-27 receptor subunit alpha"
FT                   /id="PRO_0000010877"
FT   TOPO_DOM        25..510
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        511..531
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        532..623
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          30..124
FT                   /note="Fibronectin type-III 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   DOMAIN          125..225
FT                   /note="Fibronectin type-III 2"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   DOMAIN          316..412
FT                   /note="Fibronectin type-III 3"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   DOMAIN          413..505
FT                   /note="Fibronectin type-III 4"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00316"
FT   MOTIF           211..215
FT                   /note="WSXWS motif"
FT   MOTIF           552..560
FT                   /note="Box 1 motif"
FT   CARBOHYD        46
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        296
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        305
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        360
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        368
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        461
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        156..158
FT                   /note="VLI -> ALT (in Ref. 1; AAC40121, 2; AAG27298 and 4;
FT                   AAH32878)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        170
FT                   /note="T -> A (in Ref. 1; AAC40121, 2; AAG27298 and 4;
FT                   AAH32878)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        476
FT                   /note="L -> S (in Ref. 1; AAC40121, 2; AAG27298 and 4;
FT                   AAH32878)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        497
FT                   /note="N -> D (in Ref. 1; AAC40121, 2; AAG27298 and 4;
FT                   AAH32878)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   623 AA;  69093 MW;  D683D282480242F0 CRC64;
     MNRLRVARLT PLELLLSLMS LLLGTRPHGS PGPLQCYSVG PLGILNCSWE PLGDLETPPV
     LYHQSQKYHP NRVWEVKVPS KQSWVTIPRE QFTMADKLLI WGTQKGRPLW SSVSVNLETQ
     MKPDTPQIFS QVDISEEATL EATVQWAPPV WPPQKVLICQ FRYKECQAET WTRLEPQLKT
     DGLTPVEMQN LEPGTCYQVS GRCQVENGYP WGEWSSPLSF QTPFLDPEDV WVSGTVCETS
     GKRAALLVWK DPRPCVQVTY TVWFGAGDIT TTQEEVPCCK SPVPAWMEWA VVSPGNSTSW
     VPPTNLSLVC LAPESAPCDV GVSSADGSPG IKVTWKQGTR KPLEYVVDWA QDGDSLDKLN
     WTRLPPGNLS TLLPGEFKGG VPYRITVTAV YSGGLAAAPS VWGFREELVP LAGPAVWRLP
     DDPPGTPVVA WGEVPRHQLR GQATHYTFCI QSRGLSTVCR NVSSQTQTAT LPNLHLGSFK
     LWVTVSTVAG QGPPGPNLSL HLPDNRIRWK ALPWFLSLWG LLLMGCGLSL ASTRCLQARC
     LHWRHKLLPQ WIWERVPDPA NSNSGQPYIK EVSLPQPPKD GPILEVEEVE LQPVVESPKA
     SAPIYSGYEK HFLPTPEELG LLV
//