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O70394 (I27RA_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Interleukin-27 receptor subunit alpha

Short name=IL-27 receptor subunit alpha
Short name=IL-27R subunit alpha
Short name=IL-27R-alpha
Short name=IL-27RA
Alternative name(s):
Type I T-cell cytokine receptor
Short name=TCCR
WSX-1
Gene names
Name:Il27ra
Synonyms:Tccr, Wsx1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length623 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.

Subcellular location

Membrane; Single-pass type I membrane protein.

Tissue specificity

Expressed in CD4+ and CD8+ T-cells, B-cells, natural killer cells and macrophages. Highest levels in CD4+ T-cells and natural killer cells. Expression highest in Th0 cells. Ref.2

Induction

Down-regulated on differentiation of CD4+ T-cells in both Th1 and TH2 cells.

Domain

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.

The box 1 motif is required for JAK interaction and/or activation.

Sequence similarities

Belongs to the type I cytokine receptor family. Type 2 subfamily.

Contains 4 fibronectin type-III domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424 Potential
Chain25 – 623599Interleukin-27 receptor subunit alpha
PRO_0000010877

Regions

Topological domain25 – 510486Extracellular Potential
Transmembrane511 – 53121Helical; Potential
Topological domain532 – 62392Cytoplasmic Potential
Domain30 – 12495Fibronectin type-III 1
Domain125 – 225101Fibronectin type-III 2
Domain316 – 41297Fibronectin type-III 3
Domain413 – 50593Fibronectin type-III 4
Motif211 – 2155WSXWS motif
Motif552 – 5609Box 1 motif

Amino acid modifications

Glycosylation461N-linked (GlcNAc...) Potential
Glycosylation2961N-linked (GlcNAc...) Potential
Glycosylation3051N-linked (GlcNAc...) Potential
Glycosylation3601N-linked (GlcNAc...) Potential
Glycosylation3681N-linked (GlcNAc...) Potential
Glycosylation4611N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict156 – 1583VLI → ALT in AAC40121. Ref.1
Sequence conflict156 – 1583VLI → ALT in AAG27298. Ref.2
Sequence conflict156 – 1583VLI → ALT in AAH32878. Ref.4
Sequence conflict1701T → A in AAC40121. Ref.1
Sequence conflict1701T → A in AAG27298. Ref.2
Sequence conflict1701T → A in AAH32878. Ref.4
Sequence conflict4761L → S in AAC40121. Ref.1
Sequence conflict4761L → S in AAG27298. Ref.2
Sequence conflict4761L → S in AAH32878. Ref.4
Sequence conflict4971N → D in AAC40121. Ref.1
Sequence conflict4971N → D in AAG27298. Ref.2
Sequence conflict4971N → D in AAH32878. Ref.4

Sequences

Sequence LengthMass (Da)Tools
O70394 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: D683D282480242F0

FASTA62369,093
        10         20         30         40         50         60 
MNRLRVARLT PLELLLSLMS LLLGTRPHGS PGPLQCYSVG PLGILNCSWE PLGDLETPPV 

        70         80         90        100        110        120 
LYHQSQKYHP NRVWEVKVPS KQSWVTIPRE QFTMADKLLI WGTQKGRPLW SSVSVNLETQ 

       130        140        150        160        170        180 
MKPDTPQIFS QVDISEEATL EATVQWAPPV WPPQKVLICQ FRYKECQAET WTRLEPQLKT 

       190        200        210        220        230        240 
DGLTPVEMQN LEPGTCYQVS GRCQVENGYP WGEWSSPLSF QTPFLDPEDV WVSGTVCETS 

       250        260        270        280        290        300 
GKRAALLVWK DPRPCVQVTY TVWFGAGDIT TTQEEVPCCK SPVPAWMEWA VVSPGNSTSW 

       310        320        330        340        350        360 
VPPTNLSLVC LAPESAPCDV GVSSADGSPG IKVTWKQGTR KPLEYVVDWA QDGDSLDKLN 

       370        380        390        400        410        420 
WTRLPPGNLS TLLPGEFKGG VPYRITVTAV YSGGLAAAPS VWGFREELVP LAGPAVWRLP 

       430        440        450        460        470        480 
DDPPGTPVVA WGEVPRHQLR GQATHYTFCI QSRGLSTVCR NVSSQTQTAT LPNLHLGSFK 

       490        500        510        520        530        540 
LWVTVSTVAG QGPPGPNLSL HLPDNRIRWK ALPWFLSLWG LLLMGCGLSL ASTRCLQARC 

       550        560        570        580        590        600 
LHWRHKLLPQ WIWERVPDPA NSNSGQPYIK EVSLPQPPKD GPILEVEEVE LQPVVESPKA 

       610        620 
SAPIYSGYEK HFLPTPEELG LLV 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and characterization of a novel class I cytokine receptor."
Sprecher C.A., Grant F.J., Baumgartner J.W., Presnell S.R., Schrader S.K., Yamagiwa T., Whitmore T.E., O'Hara P.J., Foster D.F.
Biochem. Biophys. Res. Commun. 246:82-90(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Spleen.
[2]"Development of Th1-type immune responses requires the type I cytokine receptor TCCR."
Chen Q., Ghilardi N., Wang H., Baker T., Xie M.-H., Gurney A., Grewal I.S., de Sauvage F.J.
Nature 407:916-920(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Spleen.
[3]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Mammary gland.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF053005 mRNA. Translation: AAC40121.1.
AF265243 mRNA. Translation: AAG27298.1.
AC156028 Genomic DNA. No translation available.
BC032878 mRNA. Translation: AAH32878.1.
RefSeqNP_057880.2. NM_016671.3.
UniGeneMm.38386.

3D structure databases

ProteinModelPortalO70394.
SMRO70394. Positions 33-503.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10090.ENSMUSP00000005601.

PTM databases

PhosphoSiteO70394.

Proteomic databases

PRIDEO70394.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000005601; ENSMUSP00000005601; ENSMUSG00000005465.
GeneID50931.
KEGGmmu:50931.
UCSCuc009mlr.2. mouse.

Organism-specific databases

CTD9466.
MGIMGI:1355318. Il27ra.

Phylogenomic databases

eggNOGNOG44355.
GeneTreeENSGT00700000104610.
HOGENOMHOG000036880.
HOVERGENHBG052068.
InParanoidO70394.
OMAPCELWVT.
OrthoDBEOG7SBNND.
TreeFamTF338122.

Gene expression databases

BgeeO70394.
CleanExMM_IL27RA.
GenevestigatorO70394.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR003961. Fibronectin_type3.
IPR013783. Ig-like_fold.
[Graphical view]
SMARTSM00060. FN3. 3 hits.
[Graphical view]
SUPFAMSSF49265. SSF49265. 2 hits.
PROSITEPS50853. FN3. 4 hits.
[Graphical view]
ProtoNetSearch...

Other

NextBio307955.
PROO70394.
SOURCESearch...

Entry information

Entry nameI27RA_MOUSE
AccessionPrimary (citable) accession number: O70394
Secondary accession number(s): E9QNY4
Entry history
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 103 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot