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Protein

Interleukin-27 receptor subunit alpha

Gene

Il27ra

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.

GO - Molecular functioni

  • interleukin-27 receptor activity Source: MGI

GO - Biological processi

  • defense response to Gram-positive bacterium Source: MGI
  • interleukin-27-mediated signaling pathway Source: GOC
  • negative regulation of type 2 immune response Source: MGI
  • positive regulation of interferon-gamma production Source: MGI
  • positive regulation of T-helper 1 type immune response Source: MGI
  • regulation of isotype switching to IgG isotypes Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-27 receptor subunit alpha
Short name:
IL-27 receptor subunit alpha
Short name:
IL-27R subunit alpha
Short name:
IL-27R-alpha
Short name:
IL-27RA
Alternative name(s):
Type I T-cell cytokine receptor
Short name:
TCCR
WSX-1
Gene namesi
Name:Il27ra
Synonyms:Tccr, Wsx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1355318. Il27ra.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini25 – 510486ExtracellularSequence AnalysisAdd
BLAST
Transmembranei511 – 53121HelicalSequence AnalysisAdd
BLAST
Topological domaini532 – 62392CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Sequence AnalysisAdd
BLAST
Chaini25 – 623599Interleukin-27 receptor subunit alphaPRO_0000010877Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi46 – 461N-linked (GlcNAc...)Sequence Analysis
Glycosylationi296 – 2961N-linked (GlcNAc...)Sequence Analysis
Glycosylationi305 – 3051N-linked (GlcNAc...)Sequence Analysis
Glycosylationi360 – 3601N-linked (GlcNAc...)Sequence Analysis
Glycosylationi368 – 3681N-linked (GlcNAc...)Sequence Analysis
Glycosylationi461 – 4611N-linked (GlcNAc...)Sequence Analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PRIDEiO70394.

PTM databases

PhosphoSiteiO70394.

Expressioni

Tissue specificityi

Expressed in CD4+ and CD8+ T-cells, B-cells, natural killer cells and macrophages. Highest levels in CD4+ T-cells and natural killer cells. Expression highest in Th0 cells.1 Publication

Inductioni

Down-regulated on differentiation of CD4+ T-cells in both Th1 and TH2 cells.

Gene expression databases

BgeeiO70394.
CleanExiMM_IL27RA.
GenevisibleiO70394. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005601.

Structurei

3D structure databases

ProteinModelPortaliO70394.
SMRiO70394. Positions 120-503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini30 – 12495Fibronectin type-III 1PROSITE-ProRule annotationAdd
BLAST
Domaini125 – 225101Fibronectin type-III 2PROSITE-ProRule annotationAdd
BLAST
Domaini316 – 41297Fibronectin type-III 3PROSITE-ProRule annotationAdd
BLAST
Domaini413 – 50593Fibronectin type-III 4PROSITE-ProRule annotationAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi211 – 2155WSXWS motif
Motifi552 – 5609Box 1 motif

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG44355.
GeneTreeiENSGT00700000104610.
HOGENOMiHOG000036880.
HOVERGENiHBG052068.
InParanoidiO70394.
OMAiWVRLPPG.
OrthoDBiEOG7SBNND.
TreeFamiTF338122.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
[Graphical view]
SMARTiSM00060. FN3. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRLRVARLT PLELLLSLMS LLLGTRPHGS PGPLQCYSVG PLGILNCSWE
60 70 80 90 100
PLGDLETPPV LYHQSQKYHP NRVWEVKVPS KQSWVTIPRE QFTMADKLLI
110 120 130 140 150
WGTQKGRPLW SSVSVNLETQ MKPDTPQIFS QVDISEEATL EATVQWAPPV
160 170 180 190 200
WPPQKVLICQ FRYKECQAET WTRLEPQLKT DGLTPVEMQN LEPGTCYQVS
210 220 230 240 250
GRCQVENGYP WGEWSSPLSF QTPFLDPEDV WVSGTVCETS GKRAALLVWK
260 270 280 290 300
DPRPCVQVTY TVWFGAGDIT TTQEEVPCCK SPVPAWMEWA VVSPGNSTSW
310 320 330 340 350
VPPTNLSLVC LAPESAPCDV GVSSADGSPG IKVTWKQGTR KPLEYVVDWA
360 370 380 390 400
QDGDSLDKLN WTRLPPGNLS TLLPGEFKGG VPYRITVTAV YSGGLAAAPS
410 420 430 440 450
VWGFREELVP LAGPAVWRLP DDPPGTPVVA WGEVPRHQLR GQATHYTFCI
460 470 480 490 500
QSRGLSTVCR NVSSQTQTAT LPNLHLGSFK LWVTVSTVAG QGPPGPNLSL
510 520 530 540 550
HLPDNRIRWK ALPWFLSLWG LLLMGCGLSL ASTRCLQARC LHWRHKLLPQ
560 570 580 590 600
WIWERVPDPA NSNSGQPYIK EVSLPQPPKD GPILEVEEVE LQPVVESPKA
610 620
SAPIYSGYEK HFLPTPEELG LLV
Length:623
Mass (Da):69,093
Last modified:July 27, 2011 - v2
Checksum:iD683D282480242F0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti156 – 1583VLI → ALT in AAC40121 (PubMed:9600072).Curated
Sequence conflicti156 – 1583VLI → ALT in AAG27298 (PubMed:11057672).Curated
Sequence conflicti156 – 1583VLI → ALT in AAH32878 (PubMed:15489334).Curated
Sequence conflicti170 – 1701T → A in AAC40121 (PubMed:9600072).Curated
Sequence conflicti170 – 1701T → A in AAG27298 (PubMed:11057672).Curated
Sequence conflicti170 – 1701T → A in AAH32878 (PubMed:15489334).Curated
Sequence conflicti476 – 4761L → S in AAC40121 (PubMed:9600072).Curated
Sequence conflicti476 – 4761L → S in AAG27298 (PubMed:11057672).Curated
Sequence conflicti476 – 4761L → S in AAH32878 (PubMed:15489334).Curated
Sequence conflicti497 – 4971N → D in AAC40121 (PubMed:9600072).Curated
Sequence conflicti497 – 4971N → D in AAG27298 (PubMed:11057672).Curated
Sequence conflicti497 – 4971N → D in AAH32878 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053005 mRNA. Translation: AAC40121.1.
AF265243 mRNA. Translation: AAG27298.1.
AC156028 Genomic DNA. No translation available.
BC032878 mRNA. Translation: AAH32878.1.
CCDSiCCDS22465.1.
RefSeqiNP_057880.2. NM_016671.3.
UniGeneiMm.38386.

Genome annotation databases

EnsembliENSMUST00000005601; ENSMUSP00000005601; ENSMUSG00000005465.
GeneIDi50931.
KEGGimmu:50931.
UCSCiuc009mlr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053005 mRNA. Translation: AAC40121.1.
AF265243 mRNA. Translation: AAG27298.1.
AC156028 Genomic DNA. No translation available.
BC032878 mRNA. Translation: AAH32878.1.
CCDSiCCDS22465.1.
RefSeqiNP_057880.2. NM_016671.3.
UniGeneiMm.38386.

3D structure databases

ProteinModelPortaliO70394.
SMRiO70394. Positions 120-503.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005601.

PTM databases

PhosphoSiteiO70394.

Proteomic databases

PRIDEiO70394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005601; ENSMUSP00000005601; ENSMUSG00000005465.
GeneIDi50931.
KEGGimmu:50931.
UCSCiuc009mlr.2. mouse.

Organism-specific databases

CTDi9466.
MGIiMGI:1355318. Il27ra.

Phylogenomic databases

eggNOGiNOG44355.
GeneTreeiENSGT00700000104610.
HOGENOMiHOG000036880.
HOVERGENiHBG052068.
InParanoidiO70394.
OMAiWVRLPPG.
OrthoDBiEOG7SBNND.
TreeFamiTF338122.

Miscellaneous databases

NextBioi307955.
PROiO70394.
SOURCEiSearch...

Gene expression databases

BgeeiO70394.
CleanExiMM_IL27RA.
GenevisibleiO70394. MM.

Family and domain databases

Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
[Graphical view]
SMARTiSM00060. FN3. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Spleen.
  2. "Development of Th1-type immune responses requires the type I cytokine receptor TCCR."
    Chen Q., Ghilardi N., Wang H., Baker T., Xie M.-H., Gurney A., Grewal I.S., de Sauvage F.J.
    Nature 407:916-920(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
    Tissue: Spleen.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Mammary gland.

Entry informationi

Entry nameiI27RA_MOUSE
AccessioniPrimary (citable) accession number: O70394
Secondary accession number(s): E9QNY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 27, 2011
Last modified: June 24, 2015
This is version 110 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.