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Protein

Interleukin-27 receptor subunit alpha

Gene

Il27ra

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Receptor for IL27. Requires IL6ST/gp130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.

GO - Molecular functioni

  • interleukin-27 receptor activity Source: MGI

GO - Biological processi

  • defense response to Gram-positive bacterium Source: MGI
  • negative regulation of type 2 immune response Source: MGI
  • positive regulation of interferon-gamma production Source: MGI
  • positive regulation of T-helper 1 type immune response Source: MGI
  • regulation of isotype switching to IgG isotypes Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Receptor

Enzyme and pathway databases

ReactomeiR-MMU-6788467. IL-6-type cytokine receptor ligand interactions.

Names & Taxonomyi

Protein namesi
Recommended name:
Interleukin-27 receptor subunit alpha
Short name:
IL-27 receptor subunit alpha
Short name:
IL-27R subunit alpha
Short name:
IL-27R-alpha
Short name:
IL-27RA
Alternative name(s):
Type I T-cell cytokine receptor
Short name:
TCCR
WSX-1
Gene namesi
Name:Il27ra
Synonyms:Tccr, Wsx1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1355318. Il27ra.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini25 – 510ExtracellularSequence analysisAdd BLAST486
Transmembranei511 – 531HelicalSequence analysisAdd BLAST21
Topological domaini532 – 623CytoplasmicSequence analysisAdd BLAST92

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000001087725 – 623Interleukin-27 receptor subunit alphaAdd BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi46N-linked (GlcNAc...)Sequence analysis1
Glycosylationi296N-linked (GlcNAc...)Sequence analysis1
Glycosylationi305N-linked (GlcNAc...)Sequence analysis1
Glycosylationi360N-linked (GlcNAc...)Sequence analysis1
Glycosylationi368N-linked (GlcNAc...)Sequence analysis1
Glycosylationi461N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiO70394.
PaxDbiO70394.
PRIDEiO70394.

PTM databases

PhosphoSitePlusiO70394.
SwissPalmiO70394.

Expressioni

Tissue specificityi

Expressed in CD4+ and CD8+ T-cells, B-cells, natural killer cells and macrophages. Highest levels in CD4+ T-cells and natural killer cells. Expression highest in Th0 cells.1 Publication

Inductioni

Down-regulated on differentiation of CD4+ T-cells in both Th1 and TH2 cells.

Gene expression databases

BgeeiENSMUSG00000005465.
CleanExiMM_IL27RA.
GenevisibleiO70394. MM.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005601.

Structurei

3D structure databases

ProteinModelPortaliO70394.
SMRiO70394.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini30 – 124Fibronectin type-III 1PROSITE-ProRule annotationAdd BLAST95
Domaini125 – 225Fibronectin type-III 2PROSITE-ProRule annotationAdd BLAST101
Domaini316 – 412Fibronectin type-III 3PROSITE-ProRule annotationAdd BLAST97
Domaini413 – 505Fibronectin type-III 4PROSITE-ProRule annotationAdd BLAST93

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi211 – 215WSXWS motif5
Motifi552 – 560Box 1 motif9

Domaini

The WSXWS motif appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding.
The box 1 motif is required for JAK interaction and/or activation.

Sequence similaritiesi

Contains 4 fibronectin type-III domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IK7Q. Eukaryota.
ENOG411182T. LUCA.
GeneTreeiENSGT00700000104610.
HOGENOMiHOG000036880.
HOVERGENiHBG052068.
InParanoidiO70394.
KOiK19598.
OMAiKVLICQF.
OrthoDBiEOG091G0BPO.
TreeFamiTF338122.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
[Graphical view]
SMARTiSM00060. FN3. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70394-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRLRVARLT PLELLLSLMS LLLGTRPHGS PGPLQCYSVG PLGILNCSWE
60 70 80 90 100
PLGDLETPPV LYHQSQKYHP NRVWEVKVPS KQSWVTIPRE QFTMADKLLI
110 120 130 140 150
WGTQKGRPLW SSVSVNLETQ MKPDTPQIFS QVDISEEATL EATVQWAPPV
160 170 180 190 200
WPPQKVLICQ FRYKECQAET WTRLEPQLKT DGLTPVEMQN LEPGTCYQVS
210 220 230 240 250
GRCQVENGYP WGEWSSPLSF QTPFLDPEDV WVSGTVCETS GKRAALLVWK
260 270 280 290 300
DPRPCVQVTY TVWFGAGDIT TTQEEVPCCK SPVPAWMEWA VVSPGNSTSW
310 320 330 340 350
VPPTNLSLVC LAPESAPCDV GVSSADGSPG IKVTWKQGTR KPLEYVVDWA
360 370 380 390 400
QDGDSLDKLN WTRLPPGNLS TLLPGEFKGG VPYRITVTAV YSGGLAAAPS
410 420 430 440 450
VWGFREELVP LAGPAVWRLP DDPPGTPVVA WGEVPRHQLR GQATHYTFCI
460 470 480 490 500
QSRGLSTVCR NVSSQTQTAT LPNLHLGSFK LWVTVSTVAG QGPPGPNLSL
510 520 530 540 550
HLPDNRIRWK ALPWFLSLWG LLLMGCGLSL ASTRCLQARC LHWRHKLLPQ
560 570 580 590 600
WIWERVPDPA NSNSGQPYIK EVSLPQPPKD GPILEVEEVE LQPVVESPKA
610 620
SAPIYSGYEK HFLPTPEELG LLV
Length:623
Mass (Da):69,093
Last modified:July 27, 2011 - v2
Checksum:iD683D282480242F0
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti156 – 158VLI → ALT in AAC40121 (PubMed:9600072).Curated3
Sequence conflicti156 – 158VLI → ALT in AAG27298 (PubMed:11057672).Curated3
Sequence conflicti156 – 158VLI → ALT in AAH32878 (PubMed:15489334).Curated3
Sequence conflicti170T → A in AAC40121 (PubMed:9600072).Curated1
Sequence conflicti170T → A in AAG27298 (PubMed:11057672).Curated1
Sequence conflicti170T → A in AAH32878 (PubMed:15489334).Curated1
Sequence conflicti476L → S in AAC40121 (PubMed:9600072).Curated1
Sequence conflicti476L → S in AAG27298 (PubMed:11057672).Curated1
Sequence conflicti476L → S in AAH32878 (PubMed:15489334).Curated1
Sequence conflicti497N → D in AAC40121 (PubMed:9600072).Curated1
Sequence conflicti497N → D in AAG27298 (PubMed:11057672).Curated1
Sequence conflicti497N → D in AAH32878 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053005 mRNA. Translation: AAC40121.1.
AF265243 mRNA. Translation: AAG27298.1.
AC156028 Genomic DNA. No translation available.
BC032878 mRNA. Translation: AAH32878.1.
CCDSiCCDS22465.1.
RefSeqiNP_057880.2. NM_016671.3.
UniGeneiMm.38386.

Genome annotation databases

EnsembliENSMUST00000005601; ENSMUSP00000005601; ENSMUSG00000005465.
GeneIDi50931.
KEGGimmu:50931.
UCSCiuc009mlr.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF053005 mRNA. Translation: AAC40121.1.
AF265243 mRNA. Translation: AAG27298.1.
AC156028 Genomic DNA. No translation available.
BC032878 mRNA. Translation: AAH32878.1.
CCDSiCCDS22465.1.
RefSeqiNP_057880.2. NM_016671.3.
UniGeneiMm.38386.

3D structure databases

ProteinModelPortaliO70394.
SMRiO70394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000005601.

PTM databases

PhosphoSitePlusiO70394.
SwissPalmiO70394.

Proteomic databases

EPDiO70394.
PaxDbiO70394.
PRIDEiO70394.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005601; ENSMUSP00000005601; ENSMUSG00000005465.
GeneIDi50931.
KEGGimmu:50931.
UCSCiuc009mlr.2. mouse.

Organism-specific databases

CTDi9466.
MGIiMGI:1355318. Il27ra.

Phylogenomic databases

eggNOGiENOG410IK7Q. Eukaryota.
ENOG411182T. LUCA.
GeneTreeiENSGT00700000104610.
HOGENOMiHOG000036880.
HOVERGENiHBG052068.
InParanoidiO70394.
KOiK19598.
OMAiKVLICQF.
OrthoDBiEOG091G0BPO.
TreeFamiTF338122.

Enzyme and pathway databases

ReactomeiR-MMU-6788467. IL-6-type cytokine receptor ligand interactions.

Miscellaneous databases

PROiO70394.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005465.
CleanExiMM_IL27RA.
GenevisibleiO70394. MM.

Family and domain databases

CDDicd00063. FN3. 1 hit.
Gene3Di2.60.40.10. 1 hit.
InterProiIPR003961. FN3_dom.
IPR013783. Ig-like_fold.
[Graphical view]
SMARTiSM00060. FN3. 3 hits.
[Graphical view]
SUPFAMiSSF49265. SSF49265. 2 hits.
PROSITEiPS50853. FN3. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiI27RA_MOUSE
AccessioniPrimary (citable) accession number: O70394
Secondary accession number(s): E9QNY4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 29, 2005
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 124 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.