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Protein

Protein CBFA2T2

Gene

Cbfa2t2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Via association with PRDM14 is involved in regulation of embryonic stem cell (ESC) pluripotency. Involved in primordial germ cell (PCG) formation (PubMed:27281218). Stabilizes PRDM14 and OCT4 on chromatin in a homooligomerization-dependent mannerCan repress the expression of MMP7 in a ZBTB33-dependent manner (By similarity). Through heteromerization with CBFA2T3/MTG16 may be involved in regulation of the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:19799863). Required for the maintenance of the secretory cell lineage in the small intestine (PubMed:16227606). Can inhibit Notch signaling probably by association with RBPJ and may be involved in GFI1-mediated Paneth cell differentiation (PubMed:25398765).By similarity3 Publications

Miscellaneous

Loss of Mtgr1 impairs the maturation of secretory cells in the small intestine.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri498 – 534MYND-typePROSITE-ProRule annotationAdd BLAST37

GO - Molecular functioni

GO - Biological processi

  • epithelial cell differentiation Source: MGI
  • intestinal epithelial cell differentiation Source: UniProtKB
  • negative regulation of neuron projection development Source: UniProtKB
  • negative regulation of Notch signaling pathway Source: UniProtKB
  • negative regulation of transcription, DNA-templated Source: MGI
  • negative regulation of transcription from RNA polymerase II promoter Source: MGI
  • positive regulation of neuron projection development Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW

Keywordsi

Molecular functionRepressor
Biological processTranscription, Transcription regulation
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Protein CBFA2T2
Alternative name(s):
MTG8-like protein
MTG8-related protein 1
Gene namesi
Name:Cbfa2t2
Synonyms:Cbfa2t2h, Mtgr1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1333833. Cbfa2t2.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Involvement in diseasei

Required for tumorigenesis in a AOM/DSS colitis-associated carcinoma model. May be involved in intestinal tumorigenesis.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi105R → D: Disrupts interaction with PRDM14. 1 Publication1
Mutagenesisi109K → E: Disrupts interaction with PRDM14. 1 Publication1
Mutagenesisi330L → E: Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-342; R-345; E-346; E-360; R-363 and R-374. 1 Publication1
Mutagenesisi342L → R: Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-345; E-346; E-360; R-363 and R-374. 1 Publication1
Mutagenesisi345A → R: Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-342; E-346; E-360; R-363 and R-374. 1 Publication1
Mutagenesisi346L → E: Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-342; R-345; R-363 and R-374. 1 Publication1
Mutagenesisi360M → E: Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-342; R-345; E-346; E-360; R-363 and R-374. 1 Publication1
Mutagenesisi363L → R: Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-342; R-345; E-346; E-360 and R-374. 1 Publication1
Mutagenesisi374L → R: Reduces PRDM14 and OCT14 occupancy on target sites, no effect on interaction with PRDM14, predicted to impair homooligomerization; when associated with R-330; R-342; R-345; E-346; E-360 and R-363. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002183021 – 594Protein CBFA2T2Add BLAST594

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei24PhosphoserineBy similarity1
Cross-linki29Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei255PhosphoserineBy similarity1
Modified residuei400PhosphoserineBy similarity1
Cross-linki440Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei567PhosphoserineBy similarity1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiO70374.
PaxDbiO70374.
PeptideAtlasiO70374.
PRIDEiO70374.

PTM databases

iPTMnetiO70374.
PhosphoSitePlusiO70374.

Expressioni

Tissue specificityi

Expressed in embryonic stem cells.1 Publication

Developmental stagei

First expression detected on embryonic day 11.5.1 Publication

Gene expression databases

BgeeiENSMUSG00000038533.
ExpressionAtlasiO70374. baseline and differential.
GenevisibleiO70374. MM.

Interactioni

Subunit structurei

Homooligomer. Homotetramerization is mediated by the NHR2 domain. Interacts with CBFA2T3/MTG16 (PubMed:19799863). Can interact with RUNX1T1/CBFA2T1. Heterotetramerization between members of the CBFA2T family is proposed (By similarity). Interacts with RBP, GFI1, TCF4, PRDM14 (PubMed:25398765, PubMed:18039847, PubMed:27281218). Interacts with TAL1 and CBFA2T3/MTG16; the heteromer with CBFA2T3/MTG16 may function in repression of TAL1 (PubMed:19799863).By similarity4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
Tal1P220918EBI-8006755,EBI-8006437

Protein-protein interaction databases

DIPiDIP-62032N.
IntActiO70374. 2 interactors.
MINTiMINT-2568187.
STRINGi10090.ENSMUSP00000043087.

Structurei

Secondary structure

1594
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi106 – 124Combined sources19
Helixi126 – 140Combined sources15
Helixi146 – 157Combined sources12
Helixi165 – 181Combined sources17

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5ECJX-ray3.05A/B98-206[»]
ProteinModelPortaliO70374.
SMRiO70374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini104 – 199TAFHPROSITE-ProRule annotationAdd BLAST96

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni98 – 206Interaction with PRDM142 PublicationsAdd BLAST109
Regioni322 – 368Nervy homology region 2 (NHR2)By similarityAdd BLAST47
Regioni426 – 475Nervy homology region 3 (NHR3)By similarityAdd BLAST50

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili442 – 482Sequence analysisAdd BLAST41

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi36 – 88Pro-richAdd BLAST53

Domaini

Nervy homology region 2 (NHR2) mediates homo- and possibly heterotypic oligomerization by forming a four-helix bundle tetrameric structure.By similarity

Sequence similaritiesi

Belongs to the CBFA2T family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri498 – 534MYND-typePROSITE-ProRule annotationAdd BLAST37

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiENOG410IG1Z. Eukaryota.
ENOG410XR30. LUCA.
GeneTreeiENSGT00390000013479.
HOGENOMiHOG000049245.
HOVERGENiHBG000169.
InParanoidiO70374.
OMAiHRLCGQN.
OrthoDBiEOG091G0EIC.
PhylomeDBiO70374.
TreeFamiTF106303.

Family and domain databases

InterProiView protein in InterPro
IPR013289. CBFA2T1/2/3.
IPR013291. MTGR1.
IPR014896. NHR2.
IPR003894. TAFH_NHR1.
IPR002893. Znf_MYND.
PANTHERiPTHR10379. PTHR10379. 1 hit.
PfamiView protein in Pfam
PF08788. NHR2. 1 hit.
PF07531. TAFH. 1 hit.
PF01753. zf-MYND. 1 hit.
PRINTSiPR01875. ETOFAMILY.
PR01877. MTGR1PROTEIN.
SMARTiView protein in SMART
SM00549. TAFH. 1 hit.
PROSITEiView protein in PROSITE
PS51119. TAFH. 1 hit.
PS01360. ZF_MYND_1. 1 hit.
PS50865. ZF_MYND_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: O70374-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVGVPGAAAF QLGCEKRVPA MPGSPVEVKI QSRSSPPIMP PLPPINPGGP
60 70 80 90 100
RPVSFTPTAL SNGINHSPPT LNGAPSPPQR FSNGPASSTS SALTNQQLPA
110 120 130 140 150
TCGARQLSKL KRFLTTLQQF GNDISPEIGE KVRTLVLALV NSTVTIEEFH
160 170 180 190 200
CKLQEATNFP LRPFVIPFLK ANLPLLQREL LHCARAAKQT PSQYLAQHEH
210 220 230 240 250
LLLNTSIASP ADSSELLMEV HGNGKRPSPE RRDENNFERD TVPPEPPAKR
260 270 280 290 300
VCTISPAPRH SPALTVPLMN PGGQFHPTPP PLQHYTLEDI ATSHLYREPN
310 320 330 340 350
KMLEHREVRE RHHNLSLNGG YQDELVDHRL TEREWADEWK HLDHALNCIM
360 370 380 390 400
EMVEKTRRSM AVLRRCQESD REELNYWKRR FNENTELRKT GTELVSRQHS
410 420 430 440 450
PGSTDSLSND SQREFTSRPA TGYVPVEFWK KTEEAVNKVK IQAMSEVQKA
460 470 480 490 500
VAEAEQKAFE VIATERARME QTIADVKRQA AEDAFLVINE QEESTENCWN
510 520 530 540 550
CGRKASETCS GCNIARYCGS FCQHKDWERH HRLCGQSLHG HSPHSQSRPL
560 570 580 590
LPGGRGSARS ADCSVPSPAL DKTSATTSRS STPASVTAID ANGL
Length:594
Mass (Da):65,904
Last modified:January 15, 2008 - v3
Checksum:iFCE4A6A0AFD66377
GO
Isoform 2 (identifier: O70374-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Show »
Length:574
Mass (Da):63,921
Checksum:i5385BD7F7D8A224E
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti413Missing in BAC30586 (PubMed:16141072).Curated1
Sequence conflicti413Missing in ABB02690 (PubMed:16227606).Curated1
Sequence conflicti413Missing in CAM13556 (PubMed:19468303).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0305171 – 20Missing in isoform 2. 1 PublicationAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK049206 mRNA. Translation: BAC33609.1.
AK040403 mRNA. Translation: BAC30586.1.
AK153602 mRNA. Translation: BAE32114.1.
DQ213025 mRNA. Translation: ABB02690.1.
AL772292 Genomic DNA. Translation: CAM13555.1.
AL772292 Genomic DNA. Translation: CAM13556.1.
BC064679 mRNA. Translation: AAH64679.1.
BC138410 mRNA. Translation: AAI38411.1.
BC145679 mRNA. Translation: AAI45680.1.
AF052219 Genomic DNA. Translation: AAC64697.1.
CCDSiCCDS16932.1. [O70374-1]
RefSeqiNP_033953.1. NM_009823.1.
NP_766448.1. NM_172860.2. [O70374-1]
XP_006498703.1. XM_006498640.2. [O70374-2]
UniGeneiMm.29914.

Genome annotation databases

EnsembliENSMUST00000045270; ENSMUSP00000043087; ENSMUSG00000038533. [O70374-1]
GeneIDi12396.
KEGGimmu:12396.
UCSCiuc008njf.2. mouse. [O70374-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiMTG8R_MOUSE
AccessioniPrimary (citable) accession number: O70374
Secondary accession number(s): B2RRH8, Q30BK8, Q6P288
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 29, 2003
Last sequence update: January 15, 2008
Last modified: October 25, 2017
This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families