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O70370

- CATS_MOUSE

UniProt

O70370 - CATS_MOUSE

Protein

Cathepsin S

Gene

Ctss

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 122 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
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    Functioni

    Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.

    Catalytic activityi

    Similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei147 – 1471By similarity
    Active sitei287 – 2871By similarity
    Active sitei307 – 3071By similarity

    GO - Molecular functioni

    1. cysteine-type peptidase activity Source: UniProtKB-KW
    2. peptidase activity Source: MGI

    GO - Biological processi

    1. basement membrane disassembly Source: BHF-UCL
    2. positive regulation of inflammatory response Source: MGI
    3. proteolysis Source: MGI
    4. proteolysis involved in cellular protein catabolic process Source: BHF-UCL

    Keywords - Molecular functioni

    Hydrolase, Protease, Thiol protease

    Enzyme and pathway databases

    BRENDAi3.4.22.27. 3474.
    ReactomeiREACT_196550. MHC class II antigen presentation.
    REACT_197101. Endosomal/Vacuolar pathway.
    REACT_198976. Trafficking and processing of endosomal TLR.
    REACT_199052. Degradation of the extracellular matrix.
    REACT_199112. Endosomal/Vacuolar pathway.

    Protein family/group databases

    MEROPSiC01.034.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cathepsin S (EC:3.4.22.27)
    Gene namesi
    Name:Ctss
    Synonyms:Cats
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 3

    Organism-specific databases

    MGIiMGI:107341. Ctss.

    Subcellular locationi

    GO - Cellular componenti

    1. early endosome lumen Source: Reactome
    2. lysosome Source: MGI
    3. membrane Source: MGI

    Keywords - Cellular componenti

    Lysosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1717Sequence AnalysisAdd
    BLAST
    Propeptidei18 – 122105Activation peptideSequence AnalysisPRO_0000026315Add
    BLAST
    Chaini123 – 340218Cathepsin SPRO_0000026316Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi120 – 1201N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi134 ↔ 233By similarity
    Disulfide bondi144 ↔ 189By similarity
    Disulfide bondi178 ↔ 222By similarity
    Disulfide bondi281 ↔ 329By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Zymogen

    Proteomic databases

    MaxQBiO70370.
    PaxDbiO70370.
    PRIDEiO70370.

    PTM databases

    PhosphoSiteiO70370.

    Expressioni

    Tissue specificityi

    Widely expressed with highest expression found in non-skeletal tissues. Relatively high levels found in skeletal tissues.1 Publication

    Gene expression databases

    ArrayExpressiO70370.
    BgeeiO70370.
    CleanExiMM_CTSS.
    GenevestigatoriO70370.

    Interactioni

    Structurei

    Secondary structure

    1
    340
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi129 – 1324
    Helixi147 – 16418
    Helixi172 – 1787
    Turni182 – 1843
    Helixi188 – 1903
    Helixi194 – 20411
    Beta strandi207 – 2093
    Turni210 – 2123
    Helixi226 – 2283
    Beta strandi229 – 2313
    Beta strandi235 – 2384
    Helixi244 – 25310
    Beta strandi257 – 2615
    Helixi267 – 2704
    Beta strandi287 – 29711
    Beta strandi300 – 3067
    Beta strandi318 – 3225
    Beta strandi324 – 3274
    Helixi328 – 3303
    Turni331 – 3333
    Beta strandi336 – 3394

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1M0Hmodel-A1-340[»]
    4BPVX-ray2.00A/B/C/D/E/K116-340[»]
    4BQVX-ray1.70A/B/C/D/E/F/G/H116-340[»]
    4BS5X-ray1.25A116-340[»]
    4BS6X-ray1.20A/B116-340[»]
    4BSQX-ray1.96A116-340[»]
    ProteinModelPortaliO70370.
    SMRiO70370. Positions 26-340.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase C1 family.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG4870.
    GeneTreeiENSGT00740000115447.
    HOVERGENiHBG011513.
    KOiK01368.

    Family and domain databases

    InterProiIPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view]
    PANTHERiPTHR12411. PTHR12411. 1 hit.
    PfamiPF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view]
    PRINTSiPR00705. PAPAIN.
    SMARTiSM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view]
    PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O70370-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRAPGHAAIR WLFWMPLVCS VAMEQLQRDP TLDYHWDLWK KTHEKEYKDK    50
    NEEEVRRLIW EKNLKFIMIH NLEYSMGMHT YQVGMNDMGD MTNEEILCRM 100
    GALRIPRQSP KTVTFRSYSN RTLPDTVDWR EKGCVTEVKY QGSCGACWAF 150
    SAVGALEGQL KLKTGKLISL SAQNLVDCSN EEKYGNKGCG GGYMTEAFQY 200
    IIDNGGIEAD ASYPYKATDE KCHYNSKNRA ATCSRYIQLP FGDEDALKEA 250
    VATKGPVSVG IDASHSSFFF YKSGVYDDPS CTGNVNHGVL VVGYGTLDGK 300
    DYWLVKNSWG LNFGDQGYIR MARNNKNHCG IASYCSYPEI 340
    Length:340
    Mass (Da):38,475
    Last modified:July 27, 2011 - v2
    Checksum:i10550C5106318C47
    GO

    Sequence cautioni

    The sequence CAA05360.1 differs from that shown. Reason: Frameshift at position 30.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti34 – 341Y → H in CAA05360. 1 PublicationCurated
    Sequence conflicti97 – 971L → S in AAB94925. 1 PublicationCurated
    Sequence conflicti97 – 971L → S in AAC05781. 1 PublicationCurated
    Sequence conflicti106 – 1061P → S in AAB94925. 1 PublicationCurated
    Sequence conflicti106 – 1061P → S in AAC05781. 1 PublicationCurated
    Sequence conflicti146 – 1461A → S in CAA77184. (PubMed:19468303)Curated
    Sequence conflicti218 – 2181T → M in AAB94925. 1 PublicationCurated
    Sequence conflicti218 – 2181T → M in CAA05360. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF051732
    , AF051727, AF051728, AF051729, AF051726, AF051730, AF051731 Genomic DNA. Translation: AAC05781.1.
    AF038546 mRNA. Translation: AAB94925.1.
    AJ002386 mRNA. Translation: CAA05360.1. Frameshift.
    AC092203 Genomic DNA. No translation available.
    Y18466 mRNA. Translation: CAA77184.1.
    AJ223208 mRNA. Translation: CAA11182.1.
    CCDSiCCDS50992.1.
    RefSeqiNP_067256.4. NM_021281.3.
    UniGeneiMm.3619.

    Genome annotation databases

    EnsembliENSMUST00000116304; ENSMUSP00000112006; ENSMUSG00000038642.
    GeneIDi13040.
    KEGGimmu:13040.
    UCSCiuc008qjz.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF051732
    , AF051727 , AF051728 , AF051729 , AF051726 , AF051730 , AF051731 Genomic DNA. Translation: AAC05781.1 .
    AF038546 mRNA. Translation: AAB94925.1 .
    AJ002386 mRNA. Translation: CAA05360.1 . Frameshift.
    AC092203 Genomic DNA. No translation available.
    Y18466 mRNA. Translation: CAA77184.1 .
    AJ223208 mRNA. Translation: CAA11182.1 .
    CCDSi CCDS50992.1.
    RefSeqi NP_067256.4. NM_021281.3.
    UniGenei Mm.3619.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1M0H model - A 1-340 [» ]
    4BPV X-ray 2.00 A/B/C/D/E/K 116-340 [» ]
    4BQV X-ray 1.70 A/B/C/D/E/F/G/H 116-340 [» ]
    4BS5 X-ray 1.25 A 116-340 [» ]
    4BS6 X-ray 1.20 A/B 116-340 [» ]
    4BSQ X-ray 1.96 A 116-340 [» ]
    ProteinModelPortali O70370.
    SMRi O70370. Positions 26-340.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi O70370.
    ChEMBLi CHEMBL4098.

    Protein family/group databases

    MEROPSi C01.034.

    PTM databases

    PhosphoSitei O70370.

    Proteomic databases

    MaxQBi O70370.
    PaxDbi O70370.
    PRIDEi O70370.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000116304 ; ENSMUSP00000112006 ; ENSMUSG00000038642 .
    GeneIDi 13040.
    KEGGi mmu:13040.
    UCSCi uc008qjz.2. mouse.

    Organism-specific databases

    CTDi 1520.
    MGIi MGI:107341. Ctss.

    Phylogenomic databases

    eggNOGi COG4870.
    GeneTreei ENSGT00740000115447.
    HOVERGENi HBG011513.
    KOi K01368.

    Enzyme and pathway databases

    BRENDAi 3.4.22.27. 3474.
    Reactomei REACT_196550. MHC class II antigen presentation.
    REACT_197101. Endosomal/Vacuolar pathway.
    REACT_198976. Trafficking and processing of endosomal TLR.
    REACT_199052. Degradation of the extracellular matrix.
    REACT_199112. Endosomal/Vacuolar pathway.

    Miscellaneous databases

    ChiTaRSi CTSS. mouse.
    NextBioi 282932.
    PROi O70370.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi O70370.
    Bgeei O70370.
    CleanExi MM_CTSS.
    Genevestigatori O70370.

    Family and domain databases

    InterProi IPR025661. Pept_asp_AS.
    IPR000169. Pept_cys_AS.
    IPR025660. Pept_his_AS.
    IPR013128. Peptidase_C1A.
    IPR000668. Peptidase_C1A_C.
    IPR013201. Prot_inhib_I29.
    [Graphical view ]
    PANTHERi PTHR12411. PTHR12411. 1 hit.
    Pfami PF08246. Inhibitor_I29. 1 hit.
    PF00112. Peptidase_C1. 1 hit.
    [Graphical view ]
    PRINTSi PR00705. PAPAIN.
    SMARTi SM00848. Inhibitor_I29. 1 hit.
    SM00645. Pept_C1. 1 hit.
    [Graphical view ]
    PROSITEi PS00640. THIOL_PROTEASE_ASN. 1 hit.
    PS00139. THIOL_PROTEASE_CYS. 1 hit.
    PS00639. THIOL_PROTEASE_HIS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Doh-ura K.
      Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
      Strain: 129/Sv and BALB/c.
      Tissue: Brain.
    2. Rommerskirch W.
      Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Spleen.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    4. "Cathepsin expression during skeletal development."
      Soederstroem M., Salminen H., Glumoff V., Kirschke H., Aro H., Vuorio E.
      Biochim. Biophys. Acta 1446:35-46(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 144-306, TISSUE SPECIFICITY.
      Strain: C57BL/6.
      Tissue: Cartilage.
    5. "Gene expression in scrapie. Cloning of a new scrapie-responsive gene and the identification of increased levels of seven other mRNA transcripts."
      Dandoy-Dron F., Guillo F., Benboudjema L., Deslys J.-P., Lasmesas C., Dormont D., Tovey M.G., Dron M.
      J. Biol. Chem. 273:7691-7697(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 296-340.
      Strain: C57BL/6.
      Tissue: Brain.

    Entry informationi

    Entry nameiCATS_MOUSE
    AccessioniPrimary (citable) accession number: O70370
    Secondary accession number(s): E9QK18, O54973
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 24, 2001
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 122 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. Peptidase families
      Classification of peptidase families and list of entries
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3