O70370 (CATS_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cathepsin S
EC=3.4.22.27

Gene names

Name:	Ctss
Synonyms:	Cats

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.
Catalytic activity	Similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound.
Subcellular location	Lysosome.
Tissue specificity	Widely expressed with highest expression found in non-skeletal tissues. Relatively high levels found in skeletal tissues. Ref.4
Sequence similarities	Belongs to the peptidase C1 family.
Sequence caution	The sequence CAA05360.1 differs from that shown. Reason: Frameshift at position 30.

Ontologies

Keywords
Cellular component	Lysosome
Domain	Signal
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adaptive immune response Inferred from electronic annotation. Source: Compara cellular response to thyroid hormone stimulus Inferred from electronic annotation. Source: Compara proteolysis Inferred from direct assay PubMed 11854359. Source: MGI
Cellular_component	early endosome lumen Traceable author statement. Source: Reactome lysosome Inferred from direct assay PubMed 11854359. Source: MGI membrane Inferred from direct assay PubMed 11854359. Source: MGI
Molecular_function	cysteine-type peptidase activity Inferred from electronic annotation. Source: UniProtKB-KW peptidase activity Inferred from direct assay PubMed 11854359. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 17

17

Potential

Propeptide

18 – 122

105

Activation peptide Potential

PRO_0000026315

Chain

123 – 340

218

Cathepsin S

PRO_0000026316

Sites

Active site

147

1

By similarity

Active site

287

1

By similarity

Active site

307

1

By similarity

Amino acid modifications

Glycosylation

120

1

N-linked (GlcNAc...) Potential

Disulfide bond

134 ↔ 233

By similarity

Disulfide bond

144 ↔ 189

By similarity

Disulfide bond

178 ↔ 222

By similarity

Disulfide bond

281 ↔ 329

By similarity

Experimental info

Sequence conflict

34

1

Y → H in CAA05360. Ref.2

Sequence conflict

97

1

L → S in AAB94925. Ref.1

Sequence conflict

97

1

L → S in AAC05781. Ref.1

Sequence conflict

106

1

P → S in AAB94925. Ref.1

Sequence conflict

106

1

P → S in AAC05781. Ref.1

Sequence conflict

146

1

A → S in CAA77184. Ref.3

Sequence conflict

218

1

T → M in AAB94925. Ref.1

Sequence conflict

218

1

T → M in CAA05360. Ref.2

Secondary structure

1

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340

Helix Strand Turn

Details...

Helix

30 – 40

11

Helix

48 – 74

27

Beta strand

78 – 81

4

Beta strand

87 – 89

3

Helix

91 – 97

7

Beta strand

110 – 115

6

Helix

129 – 132

4

Helix

147 – 164

18

Helix

172 – 178

7

Turn

182 – 185

4

Helix

194 – 204

11

Beta strand

207 – 209

3

Turn

210 – 212

3

Helix

226 – 228

3

Beta strand

229 – 231

3

Helix

244 – 253

10

Beta strand

257 – 261

5

Helix

266 – 269

4

Beta strand

271 – 276

6

Beta strand

288 – 298

11

Beta strand

301 – 307

7

Turn

311 – 313

3

Beta strand

319 – 323

5

Beta strand

325 – 328

4

Helix

329 – 331

3

Sequences

	Sequence		Length	Mass (Da)
	O70370 [UniParc]. Last modified July 27, 2011. Version 2. Checksum: 10550C5106318C47	FASTA	340	38,475
	10 20 30 40 50 60 MRAPGHAAIR WLFWMPLVCS VAMEQLQRDP TLDYHWDLWK KTHEKEYKDK NEEEVRRLIW 70 80 90 100 110 120 EKNLKFIMIH NLEYSMGMHT YQVGMNDMGD MTNEEILCRM GALRIPRQSP KTVTFRSYSN 130 140 150 160 170 180 RTLPDTVDWR EKGCVTEVKY QGSCGACWAF SAVGALEGQL KLKTGKLISL SAQNLVDCSN 190 200 210 220 230 240 EEKYGNKGCG GGYMTEAFQY IIDNGGIEAD ASYPYKATDE KCHYNSKNRA ATCSRYIQLP 250 260 270 280 290 300 FGDEDALKEA VATKGPVSVG IDASHSSFFF YKSGVYDDPS CTGNVNHGVL VVGYGTLDGK 310 320 330 340 DYWLVKNSWG LNFGDQGYIR MARNNKNHCG IASYCSYPEI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Doh-ura K. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA]. Strain: 129/Sv and BALB/c. Tissue: Brain.
[2]	Rommerskirch W. Submitted (NOV-1997) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Spleen.
[3]	"Lineage-specific biology revealed by a finished genome assembly of the mouse." Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P. PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: C57BL/6J.
[4]	"Cathepsin expression during skeletal development." Soederstroem M., Salminen H., Glumoff V., Kirschke H., Aro H., Vuorio E. Biochim. Biophys. Acta 1446:35-46(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 144-306, TISSUE SPECIFICITY. Strain: C57BL/6. Tissue: Cartilage.
[5]	"Gene expression in scrapie. Cloning of a new scrapie-responsive gene and the identification of increased levels of seven other mRNA transcripts." Dandoy-Dron F., Guillo F., Benboudjema L., Deslys J.-P., Lasmesas C., Dormont D., Tovey M.G., Dron M. J. Biol. Chem. 273:7691-7697(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 296-340. Strain: C57BL/6. Tissue: Brain.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

AF051732 , AF051727, AF051728, AF051729, AF051726, AF051730, AF051731 Genomic DNA. Translation: AAC05781.1.
AF038546 mRNA. Translation: AAB94925.1.
AJ002386 mRNA. Translation: CAA05360.1. Frameshift.
AC092203 Genomic DNA. No translation available.
Y18466 mRNA. Translation: CAA77184.1.
AJ223208 mRNA. Translation: CAA11182.1.

IPI

IPI00309520.

UniGene

Mm.3619.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1M0H	model	-	A	1-340	[»]

ProteinModelPortal

O70370.

SMR

O70370. Positions 26-340.

ModBase

Search...

PTM databases

PhosphoSite

O70370.

Proteomic databases

PaxDb

O70370.

PRIDE

O70370.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000116304; ENSMUSP00000112006; ENSMUSG00000038642.

Organism-specific databases

MGI

MGI:107341. Ctss.

Phylogenomic databases

eggNOG

COG4870.

GeneTree

ENSGT00560000076577.

HOVERGEN

HBG011513.

Enzyme and pathway databases

BRENDA

3.4.22.27. 3474.

Reactome

REACT_102124. Immune System.

Gene expression databases

ArrayExpress

O70370.

Bgee

O70370.

CleanEx

MM_CTSS.

Genevestigator

O70370.

GermOnline

ENSMUSG00000038642. Mus musculus.

Family and domain databases

InterPro

IPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]

PANTHER

PTHR12411. PTHR12411. 1 hit.

Pfam

PF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]

PRINTS

PR00705. PAPAIN.

SMART

SM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]

PROSITE

PS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

BindingDB

O70370.

ChEMBL

CHEMBL4098.

ChiTaRS

CTSS. mouse.

NextBio

282932.

SOURCE

Search...

Entry information

Entry name

CATS_MOUSE

Accession

Primary (citable) accession number: O70370
Secondary accession number(s): E9QK18, O54973

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 24, 2001
Last sequence update:	July 27, 2011
Last modified:	May 1, 2013
This is version 110 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program