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Protein

Cathepsin S

Gene

Ctss

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Thiol protease. Key protease responsible for the removal of the invariant chain from MHC class II molecules. The bond-specificity of this proteinase is in part similar to the specificities of cathepsin L and cathepsin N.

Catalytic activityi

Similar to cathepsin L, but with much less activity on Z-Phe-Arg-|-NHMec, and more activity on the Z-Val-Val-Arg-|-Xaa compound.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei147By similarity1
Active sitei287By similarity1
Active sitei307By similarity1

GO - Molecular functioni

GO - Biological processi

  • antigen processing and presentation Source: GO_Central
  • antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent Source: Reactome
  • antigen processing and presentation of peptide antigen Source: MGI
  • basement membrane disassembly Source: BHF-UCL
  • collagen catabolic process Source: MGI
  • immune response Source: GO_Central
  • positive regulation of cation channel activity Source: CACAO
  • positive regulation of inflammatory response Source: MGI
  • protein processing Source: MGI
  • proteolysis Source: MGI
  • proteolysis involved in cellular protein catabolic process Source: BHF-UCL
  • response to acidic pH Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Enzyme and pathway databases

BRENDAi3.4.22.27. 3474.
ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1679131. Trafficking and processing of endosomal TLR.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-6798695. Neutrophil degranulation.

Protein family/group databases

MEROPSiC01.034.

Names & Taxonomyi

Protein namesi
Recommended name:
Cathepsin S (EC:3.4.22.27)
Gene namesi
Name:Ctss
Synonyms:Cats
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:107341. Ctss.

Subcellular locationi

GO - Cellular componenti

  • early endosome lumen Source: Reactome
  • extracellular space Source: MGI
  • intracellular membrane-bounded organelle Source: MGI
  • lysosome Source: MGI
  • membrane Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Lysosome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL4098.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 17Sequence analysisAdd BLAST17
PropeptideiPRO_000002631518 – 122Activation peptideSequence analysisAdd BLAST105
ChainiPRO_0000026316123 – 340Cathepsin SAdd BLAST218

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi120N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi134 ↔ 233By similarity
Disulfide bondi144 ↔ 189By similarity
Disulfide bondi178 ↔ 222By similarity
Disulfide bondi281 ↔ 329By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

EPDiO70370.
MaxQBiO70370.
PaxDbiO70370.
PRIDEiO70370.

PTM databases

PhosphoSitePlusiO70370.

Expressioni

Tissue specificityi

Widely expressed with highest expression found in non-skeletal tissues. Relatively high levels found in skeletal tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000038642.
CleanExiMM_CTSS.
ExpressionAtlasiO70370. baseline and differential.
GenevisibleiO70370. MM.

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112006.

Chemistry databases

BindingDBiO70370.

Structurei

Secondary structure

1340
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi129 – 132Combined sources4
Helixi147 – 164Combined sources18
Helixi172 – 178Combined sources7
Turni182 – 184Combined sources3
Helixi188 – 190Combined sources3
Helixi194 – 204Combined sources11
Beta strandi207 – 209Combined sources3
Turni210 – 212Combined sources3
Helixi226 – 228Combined sources3
Beta strandi229 – 231Combined sources3
Beta strandi234 – 238Combined sources5
Helixi244 – 253Combined sources10
Beta strandi257 – 261Combined sources5
Helixi266 – 270Combined sources5
Beta strandi273 – 276Combined sources4
Beta strandi287 – 297Combined sources11
Beta strandi300 – 306Combined sources7
Beta strandi318 – 322Combined sources5
Beta strandi324 – 327Combined sources4
Helixi328 – 330Combined sources3
Turni331 – 333Combined sources3
Beta strandi336 – 339Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M0Hmodel-A1-340[»]
4BPVX-ray2.00A/B/C/D/E/K116-340[»]
4BQVX-ray1.70A/B/C/D/E/F/G/H116-340[»]
4BS5X-ray1.25A116-340[»]
4BS6X-ray1.20A/B116-340[»]
4BSQX-ray1.96A116-340[»]
4MZOX-ray1.47A/B/C/D/E/F/G/H116-340[»]
4MZSX-ray1.85A/B116-340[»]
ProteinModelPortaliO70370.
SMRiO70370.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase C1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOVERGENiHBG011513.
InParanoidiO70370.
KOiK01368.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70370-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRAPGHAAIR WLFWMPLVCS VAMEQLQRDP TLDYHWDLWK KTHEKEYKDK
60 70 80 90 100
NEEEVRRLIW EKNLKFIMIH NLEYSMGMHT YQVGMNDMGD MTNEEILCRM
110 120 130 140 150
GALRIPRQSP KTVTFRSYSN RTLPDTVDWR EKGCVTEVKY QGSCGACWAF
160 170 180 190 200
SAVGALEGQL KLKTGKLISL SAQNLVDCSN EEKYGNKGCG GGYMTEAFQY
210 220 230 240 250
IIDNGGIEAD ASYPYKATDE KCHYNSKNRA ATCSRYIQLP FGDEDALKEA
260 270 280 290 300
VATKGPVSVG IDASHSSFFF YKSGVYDDPS CTGNVNHGVL VVGYGTLDGK
310 320 330 340
DYWLVKNSWG LNFGDQGYIR MARNNKNHCG IASYCSYPEI
Length:340
Mass (Da):38,475
Last modified:July 27, 2011 - v2
Checksum:i10550C5106318C47
GO

Sequence cautioni

The sequence CAA05360 differs from that shown. Reason: Frameshift at position 30.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti34Y → H in CAA05360 (Ref. 2) Curated1
Sequence conflicti97L → S in AAB94925 (Ref. 1) Curated1
Sequence conflicti97L → S in AAC05781 (Ref. 1) Curated1
Sequence conflicti106P → S in AAB94925 (Ref. 1) Curated1
Sequence conflicti106P → S in AAC05781 (Ref. 1) Curated1
Sequence conflicti146A → S in CAA77184 (PubMed:19468303).Curated1
Sequence conflicti218T → M in AAB94925 (Ref. 1) Curated1
Sequence conflicti218T → M in CAA05360 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051732
, AF051727, AF051728, AF051729, AF051726, AF051730, AF051731 Genomic DNA. Translation: AAC05781.1.
AF038546 mRNA. Translation: AAB94925.1.
AJ002386 mRNA. Translation: CAA05360.1. Frameshift.
AC092203 Genomic DNA. No translation available.
Y18466 mRNA. Translation: CAA77184.1.
AJ223208 mRNA. Translation: CAA11182.1.
CCDSiCCDS50992.1.
RefSeqiNP_067256.4. NM_021281.3.
UniGeneiMm.3619.

Genome annotation databases

EnsembliENSMUST00000116304; ENSMUSP00000112006; ENSMUSG00000038642.
GeneIDi13040.
KEGGimmu:13040.
UCSCiuc008qjz.3. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF051732
, AF051727, AF051728, AF051729, AF051726, AF051730, AF051731 Genomic DNA. Translation: AAC05781.1.
AF038546 mRNA. Translation: AAB94925.1.
AJ002386 mRNA. Translation: CAA05360.1. Frameshift.
AC092203 Genomic DNA. No translation available.
Y18466 mRNA. Translation: CAA77184.1.
AJ223208 mRNA. Translation: CAA11182.1.
CCDSiCCDS50992.1.
RefSeqiNP_067256.4. NM_021281.3.
UniGeneiMm.3619.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1M0Hmodel-A1-340[»]
4BPVX-ray2.00A/B/C/D/E/K116-340[»]
4BQVX-ray1.70A/B/C/D/E/F/G/H116-340[»]
4BS5X-ray1.25A116-340[»]
4BS6X-ray1.20A/B116-340[»]
4BSQX-ray1.96A116-340[»]
4MZOX-ray1.47A/B/C/D/E/F/G/H116-340[»]
4MZSX-ray1.85A/B116-340[»]
ProteinModelPortaliO70370.
SMRiO70370.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000112006.

Chemistry databases

BindingDBiO70370.
ChEMBLiCHEMBL4098.

Protein family/group databases

MEROPSiC01.034.

PTM databases

PhosphoSitePlusiO70370.

Proteomic databases

EPDiO70370.
MaxQBiO70370.
PaxDbiO70370.
PRIDEiO70370.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000116304; ENSMUSP00000112006; ENSMUSG00000038642.
GeneIDi13040.
KEGGimmu:13040.
UCSCiuc008qjz.3. mouse.

Organism-specific databases

CTDi1520.
MGIiMGI:107341. Ctss.

Phylogenomic databases

eggNOGiKOG1543. Eukaryota.
COG4870. LUCA.
GeneTreeiENSGT00760000118871.
HOVERGENiHBG011513.
InParanoidiO70370.
KOiK01368.

Enzyme and pathway databases

BRENDAi3.4.22.27. 3474.
ReactomeiR-MMU-1474228. Degradation of the extracellular matrix.
R-MMU-1679131. Trafficking and processing of endosomal TLR.
R-MMU-2132295. MHC class II antigen presentation.
R-MMU-6798695. Neutrophil degranulation.

Miscellaneous databases

ChiTaRSiCtss. mouse.
PROiO70370.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038642.
CleanExiMM_CTSS.
ExpressionAtlasiO70370. baseline and differential.
GenevisibleiO70370. MM.

Family and domain databases

InterProiIPR025661. Pept_asp_AS.
IPR000169. Pept_cys_AS.
IPR025660. Pept_his_AS.
IPR013128. Peptidase_C1A.
IPR000668. Peptidase_C1A_C.
IPR013201. Prot_inhib_I29.
[Graphical view]
PANTHERiPTHR12411. PTHR12411. 1 hit.
PfamiPF08246. Inhibitor_I29. 1 hit.
PF00112. Peptidase_C1. 1 hit.
[Graphical view]
PRINTSiPR00705. PAPAIN.
SMARTiSM00848. Inhibitor_I29. 1 hit.
SM00645. Pept_C1. 1 hit.
[Graphical view]
PROSITEiPS00640. THIOL_PROTEASE_ASN. 1 hit.
PS00139. THIOL_PROTEASE_CYS. 1 hit.
PS00639. THIOL_PROTEASE_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCATS_MOUSE
AccessioniPrimary (citable) accession number: O70370
Secondary accession number(s): E9QK18, O54973
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 24, 2001
Last sequence update: July 27, 2011
Last modified: November 30, 2016
This is version 143 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.