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O70362 (PHLD_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 88. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Phosphatidylinositol-glycan-specific phospholipase D
Short name=PI-G PLD
EC=3.1.4.50
Alternative name(s):
Glycoprotein phospholipase D
Glycosyl-phosphatidylinositol-specific phospholipase D
Short name=GPI-PLD
Short name=GPI-specific phospholipase D
Gene names
Name:Gpld1
OrganismMus musculus (Mouse)
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length837 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. Ref.1

Catalytic activity

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + phosphatidate.

Subunit structure

Monomer Potential. Ref.1

Subcellular location

Secreted. Note: Associated with the High-Density Lipoproteins (HDL) By similarity.

Tissue specificity

Widely expressed. Ref.1

Sequence similarities

Belongs to the GPLD1 family.

Contains 7 FG-GAP repeats.

Ontologies

Keywords
   Cellular componentHDL
Secreted
   DomainRepeat
Signal
   Molecular functionHydrolase
   PTMGlycoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular componenthigh-density lipoprotein particle

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionglycosylphosphatidylinositol phospholipase D activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323 By similarity
Chain24 – 837814Phosphatidylinositol-glycan-specific phospholipase D
PRO_0000022054

Regions

Repeat364 – 42562FG-GAP 1
Repeat431 – 49262FG-GAP 2
Repeat494 – 55461FG-GAP 3
Repeat561 – 61959FG-GAP 4
Repeat629 – 68961FG-GAP 5
Repeat701 – 76767FG-GAP 6
Repeat785 – 83753FG-GAP 7

Amino acid modifications

Glycosylation941N-linked (GlcNAc...) Potential
Glycosylation2671N-linked (GlcNAc...) Potential
Glycosylation2871N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation3171N-linked (GlcNAc...) Ref.2
Glycosylation4771N-linked (GlcNAc...) Potential
Glycosylation4961N-linked (GlcNAc...) Ref.3
Glycosylation5861N-linked (GlcNAc...) Potential
Glycosylation5991N-linked (GlcNAc...) Potential
Glycosylation6551N-linked (GlcNAc...) Ref.2

Sequences

Sequence LengthMass (Da)Tools
O70362 [UniParc].

Last modified August 1, 1998. Version 1.
Checksum: 9D007F479556CCC1

FASTA83793,255
        10         20         30         40         50         60 
MSAGRLWSSL LLLLPLFCSK SSSCGLSTHV EIGHRALEFL RLQDGRINYK ELILEHQDAY 

        70         80         90        100        110        120 
QAGTVFPDAF YPSICKRGKY HDVSERTHWT PFLNASIHYI RENYPLPWEK DTEKLVAFLF 

       130        140        150        160        170        180 
GITSHMVADL SWHNLGFLRT MGAIDFYNSY SDAHSAGDFG GDVLSQFEFN FNYLSRRWYV 

       190        200        210        220        230        240 
PVRDLLRIYD NLYGRKVITK DVLVDCTYLQ FLEMHGEMFA VSKLYSTYST KSPFLVEQFQ 

       250        260        270        280        290        300 
DYFLGGLDDM AFWSTNIYRL TSFMLENGTS DCNLPENPLF ISCDGRNHTL SGSKVQKNDF 

       310        320        330        340        350        360 
HRNLTMFISR DIRKNLNYTE RGVFYSTGSW ARPESVTFMY QTLERNLRLM LAGSSQKNLN 

       370        380        390        400        410        420 
HVSSPSASYT LSVPYARLGW VMTSADLNQD GHGDLVVGAP GYSHPGRFQI GRVYIIYGND 

       430        440        450        460        470        480 
LGLPPIDLDL NKEGILEGFQ PSGRFGSALA VLDFNQDGLP DLAVGAPSVG SGQLTYNGSV 

       490        500        510        520        530        540 
YVYYGSQQGR LSSSPNVTIS CKDTYCNLGW TLLATDADGD GRHDLVISSP FAPGGRKQKG 

       550        560        570        580        590        600 
IVATFYSHPR RNDKELLTLE EADWKVNGEE DFSWFGYSLH GVTVANRSLL LIGSPTWKNV 

       610        620        630        640        650        660 
SRMARSSHKK NQEEKSLGKV YGYFLPNRQS TITISGDKAM GKLGTSLSSG YVRVNGTLTQ 

       670        680        690        700        710        720 
VLLVGAPTHD DVSKMAFLTM TLHQGGATRM YELAPEKTQP ALLSTFSGDR RFSRFGSVLH 

       730        740        750        760        770        780 
LTDLDDDGLD EIIMAAPLRI TDVTSGLLGG EDGRVYIYNG MYTTLGDMTG KCKSWMTPCP 

       790        800        810        820        830 
EEKAQYVLTS PEASSRFGSS LVSVRSKGRN QVVVAAGRSS WGARLSGALH VYSFSSD

« Hide

References

« Hide 'large scale' references
[1]"Mouse glycosylphosphatidylinositol-specific phospholipase D (Gpld1) characterization."
LeBoeuf R.C., Caldwell M., Guo Y., Metz C., Davitz M.A., Olson L.K., Deeg M.A.
Mamm. Genome 9:710-714(1998) [PubMed: 9716655] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, FUNCTION, TISSUE SPECIFICITY.
Tissue: Glucagonoma.
[2]"Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
J. Proteome Res. 5:2438-2447(2006) [PubMed: 16944957] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-317 AND ASN-655, MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Plasma.
[3]"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
Bernhard O.K., Kapp E.A., Simpson R.J.
J. Proteome Res. 6:987-995(2007) [PubMed: 17330941] [Abstract]
Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-496, MASS SPECTROMETRY.
Strain: C57BL/6.
Tissue: Plasma.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AF050666 mRNA. Translation: AAC77799.1.
IPIIPI00225715.
UniGeneMm.291831.

3D structure databases

ProteinModelPortalO70362.
SMRO70362. Positions 298-599, 639-836.
ModBaseSearch...

Protein-protein interaction databases

STRINGO70362.

PTM databases

PhosphoSiteO70362.

Proteomic databases

PRIDEO70362.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:106604. Gpld1.

Phylogenomic databases

eggNOGroNOG11917.
HOVERGENHBG008185.
InParanoidO70362.
OrthoDBEOG48SGSC.

Enzyme and pathway databases

BRENDA3.1.4.50. 3474.

Gene expression databases

ArrayExpressO70362.
BgeeO70362.
GenevestigatorO70362.
GermOnlineENSMUSG00000021340. Mus musculus.

Family and domain databases

InterProIPR013517. FG-GAP.
IPR001028. Gprt_PLipase_D.
IPR013519. Int_alpha_beta-p.
[Graphical view]
PfamPF01839. FG-GAP. 2 hits.
[Graphical view]
PRINTSPR00718. PHPHLIPASED.
SMARTSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEPS51470. FG_GAP. 7 hits.
[Graphical view]
ProtoNetSearch...

Other

SOURCESearch...

Entry information

Entry namePHLD_MOUSE
AccessionPrimary (citable) accession number: O70362
Entry history
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: October 19, 2011
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

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