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Protein

Phosphatidylinositol-glycan-specific phospholipase D

Gene

Gpld1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane.1 Publication

Catalytic activityi

6-(alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol + H2O = 6-(alpha-D-glucosaminyl)-1D-myo-inositol + phosphatidate.

GO - Molecular functioni

  • glycosylphosphatidylinositol phospholipase D activity Source: UniProtKB
  • phospholipase D activity Source: MGI
  • sodium channel regulator activity Source: UniProtKB

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Enzyme and pathway databases

BRENDAi3.1.4.50. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylinositol-glycan-specific phospholipase D (EC:3.1.4.50)
Short name:
PI-G PLD
Alternative name(s):
Glycoprotein phospholipase D
Glycosyl-phosphatidylinositol-specific phospholipase D
Short name:
GPI-PLD
Short name:
GPI-specific phospholipase D
Gene namesi
Name:Gpld1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:106604. Gpld1.

Subcellular locationi

  • Secreted

  • Note: Associated with the High-Density Lipoproteins (HDL).By similarity

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: MGI
  • extracellular region Source: UniProtKB
  • extracellular space Source: UniProtKB
  • high-density lipoprotein particle Source: UniProtKB-KW
  • intracellular Source: MGI
  • intracellular membrane-bounded organelle Source: UniProtKB
  • lysosomal membrane Source: MGI
  • proteinaceous extracellular matrix Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323By similarityAdd
BLAST
Chaini24 – 837814Phosphatidylinositol-glycan-specific phospholipase DPRO_0000022054Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi94 – 941N-linked (GlcNAc...)Sequence analysis
Glycosylationi267 – 2671N-linked (GlcNAc...)Sequence analysis
Glycosylationi287 – 2871N-linked (GlcNAc...)Sequence analysis
Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence analysis
Glycosylationi317 – 3171N-linked (GlcNAc...)1 Publication
Glycosylationi477 – 4771N-linked (GlcNAc...)Sequence analysis
Glycosylationi496 – 4961N-linked (GlcNAc...)1 Publication
Glycosylationi586 – 5861N-linked (GlcNAc...)Sequence analysis
Glycosylationi599 – 5991N-linked (GlcNAc...)Sequence analysis
Glycosylationi655 – 6551N-linked (GlcNAc...)1 Publication

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiO70362.
MaxQBiO70362.
PaxDbiO70362.
PRIDEiO70362.

PTM databases

PhosphoSiteiO70362.

Expressioni

Tissue specificityi

Widely expressed.1 Publication

Interactioni

Subunit structurei

Monomer.Curated

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021773.

Structurei

3D structure databases

ProteinModelPortaliO70362.
SMRiO70362. Positions 376-581, 615-765.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati364 – 42562FG-GAP 1PROSITE-ProRule annotationAdd
BLAST
Repeati431 – 49262FG-GAP 2PROSITE-ProRule annotationAdd
BLAST
Repeati494 – 55461FG-GAP 3PROSITE-ProRule annotationAdd
BLAST
Repeati561 – 61959FG-GAP 4PROSITE-ProRule annotationAdd
BLAST
Repeati629 – 68961FG-GAP 5PROSITE-ProRule annotationAdd
BLAST
Repeati701 – 76767FG-GAP 6PROSITE-ProRule annotationAdd
BLAST
Repeati785 – 83753FG-GAP 7PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the GPLD1 family.Curated
Contains 7 FG-GAP repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiENOG410IPB3. Eukaryota.
ENOG4111ZDA. LUCA.
HOVERGENiHBG008185.
InParanoidiO70362.

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR001028. Gprt_PLipase_D.
IPR013519. Int_alpha_beta-p.
IPR029002. PLPC/GPLD1.
[Graphical view]
PfamiPF01839. FG-GAP. 3 hits.
PF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00718. PHPHLIPASED.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70362-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSAGRLWSSL LLLLPLFCSK SSSCGLSTHV EIGHRALEFL RLQDGRINYK
60 70 80 90 100
ELILEHQDAY QAGTVFPDAF YPSICKRGKY HDVSERTHWT PFLNASIHYI
110 120 130 140 150
RENYPLPWEK DTEKLVAFLF GITSHMVADL SWHNLGFLRT MGAIDFYNSY
160 170 180 190 200
SDAHSAGDFG GDVLSQFEFN FNYLSRRWYV PVRDLLRIYD NLYGRKVITK
210 220 230 240 250
DVLVDCTYLQ FLEMHGEMFA VSKLYSTYST KSPFLVEQFQ DYFLGGLDDM
260 270 280 290 300
AFWSTNIYRL TSFMLENGTS DCNLPENPLF ISCDGRNHTL SGSKVQKNDF
310 320 330 340 350
HRNLTMFISR DIRKNLNYTE RGVFYSTGSW ARPESVTFMY QTLERNLRLM
360 370 380 390 400
LAGSSQKNLN HVSSPSASYT LSVPYARLGW VMTSADLNQD GHGDLVVGAP
410 420 430 440 450
GYSHPGRFQI GRVYIIYGND LGLPPIDLDL NKEGILEGFQ PSGRFGSALA
460 470 480 490 500
VLDFNQDGLP DLAVGAPSVG SGQLTYNGSV YVYYGSQQGR LSSSPNVTIS
510 520 530 540 550
CKDTYCNLGW TLLATDADGD GRHDLVISSP FAPGGRKQKG IVATFYSHPR
560 570 580 590 600
RNDKELLTLE EADWKVNGEE DFSWFGYSLH GVTVANRSLL LIGSPTWKNV
610 620 630 640 650
SRMARSSHKK NQEEKSLGKV YGYFLPNRQS TITISGDKAM GKLGTSLSSG
660 670 680 690 700
YVRVNGTLTQ VLLVGAPTHD DVSKMAFLTM TLHQGGATRM YELAPEKTQP
710 720 730 740 750
ALLSTFSGDR RFSRFGSVLH LTDLDDDGLD EIIMAAPLRI TDVTSGLLGG
760 770 780 790 800
EDGRVYIYNG MYTTLGDMTG KCKSWMTPCP EEKAQYVLTS PEASSRFGSS
810 820 830
LVSVRSKGRN QVVVAAGRSS WGARLSGALH VYSFSSD
Length:837
Mass (Da):93,255
Last modified:August 1, 1998 - v1
Checksum:i9D007F479556CCC1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF050666 mRNA. Translation: AAC77799.1.
UniGeneiMm.291831.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF050666 mRNA. Translation: AAC77799.1.
UniGeneiMm.291831.

3D structure databases

ProteinModelPortaliO70362.
SMRiO70362. Positions 376-581, 615-765.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021773.

PTM databases

PhosphoSiteiO70362.

Proteomic databases

EPDiO70362.
MaxQBiO70362.
PaxDbiO70362.
PRIDEiO70362.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:106604. Gpld1.

Phylogenomic databases

eggNOGiENOG410IPB3. Eukaryota.
ENOG4111ZDA. LUCA.
HOVERGENiHBG008185.
InParanoidiO70362.

Enzyme and pathway databases

BRENDAi3.1.4.50. 3474.

Miscellaneous databases

PROiO70362.
SOURCEiSearch...

Family and domain databases

InterProiIPR013517. FG-GAP.
IPR001028. Gprt_PLipase_D.
IPR013519. Int_alpha_beta-p.
IPR029002. PLPC/GPLD1.
[Graphical view]
PfamiPF01839. FG-GAP. 3 hits.
PF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00718. PHPHLIPASED.
SMARTiSM00191. Int_alpha. 5 hits.
[Graphical view]
PROSITEiPS51470. FG_GAP. 7 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mouse glycosylphosphatidylinositol-specific phospholipase D (Gpld1) characterization."
    LeBoeuf R.C., Caldwell M., Guo Y., Metz C., Davitz M.A., Olson L.K., Deeg M.A.
    Mamm. Genome 9:710-714(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], SUBUNIT, FUNCTION, TISSUE SPECIFICITY.
    Tissue: Glucagonoma.
  2. "Proteome-wide characterization of N-glycosylation events by diagonal chromatography."
    Ghesquiere B., Van Damme J., Martens L., Vandekerckhove J., Gevaert K.
    J. Proteome Res. 5:2438-2447(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-317 AND ASN-655.
    Strain: C57BL/6J.
    Tissue: Plasma.
  3. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-496.
    Strain: C57BL/6J.
    Tissue: Plasma.
  4. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Spleen and Testis.

Entry informationi

Entry nameiPHLD_MOUSE
AccessioniPrimary (citable) accession number: O70362
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: August 1, 1998
Last modified: June 8, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.