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O70361 (PER3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Period circadian protein homolog 3

Short name=mPER3
Alternative name(s):
Circadian clock protein PERIOD 3
Gene names
Name:Per3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1113 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the circadian clock mechanism which is essential for generating circadian rhythms. Can bind heme. Ref.8

Subunit structure

Homodimer. Component of the circadian core oscillator, which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Interacts directly with PER1, PER2, CRY1, CRY2, and TIMELESS. Interaction with CSNK1D or CSKN1E promotes nuclear location of PER proteins. Ref.3 Ref.5 Ref.6 Ref.7 Ref.8

Subcellular location

Cytoplasm. Nucleus. Note: Mainly cytoplasmic. Translocates to the nucleus through binding PER1, PER2, CRY1 or CRY2, but not TIMELESS. Ref.3 Ref.4 Ref.5 Ref.7

Tissue specificity

Widely expressed. Expressed in heart, brain, lung, liver, skeletal muscle, testis, and at low level in the spleen and kidney. In brain, mainly found in the SCN, hippocampus, piriform cortex, and cerebellum. Lower level of expression in the neocortex. Expression exhibits synchronous oscillations in liver, skeletal muscle and testis. Ref.1

Induction

Exhibits circadian oscillation expression in SCN, liver, skeletal muscle, testis and eyes. In the SCN, highest levles during subjective day at CT6 and CT9, lowest levels at night, CT15, CT18 and CT 21. In the liver, skeletal muscle, testis and eyes highest levels at CT15, CT15-CT18, CT9 and CT15, and CT9-CT15, respectively. During subjective night, unresponsive to light exposure. Ref.1 Ref.6 Ref.7

Post-translational modification

Phosphorylation appears to require association with PER1 and translocation to the nucleus.

Ubiquitinated. Ref.5

Sequence similarities

Contains 1 PAC (PAS-associated C-terminal) domain.

Contains 2 PAS (PER-ARNT-SIM) domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11131113Period circadian protein homolog 3
PRO_0000162634

Regions

Domain120 – 18768PAS 1
Domain258 – 32467PAS 2
Domain333 – 37644PAC
Region498 – 721224CSNK1E binding domain By similarity
Region1035 – 111379CRY binding domain By similarity
Motif396 – 40813Nuclear export signal By similarity
Motif719 – 73517Nuclear localization signal By similarity
Compositional bias562 – 5654Poly-Ser

Experimental info

Mutagenesis3591W → E: Abolishes homodimerization. Ref.8
Mutagenesis3671I → E: Abolishes homodimerization. Ref.8
Sequence conflict9671Q → H in AAC40147. Ref.1
Sequence conflict11071H → R in AAC40147. Ref.1

Secondary structure

............................................................. 1113
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
O70361 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 99B06113BAB743C7

FASTA1,113120,911
        10         20         30         40         50         60 
MDPCGDPAVP GGDCPQTRGP GLQGASGQEG PLQGTCVDSS HSEHEDRNRM SEELIMVVQE 

        70         80         90        100        110        120 
MKKYFPAERH TKPSTLDALN YALRCVHSVQ ANSDFFQSLG PRGAHQADVT VYSLEDLTAL 

       130        140        150        160        170        180 
ASEHTSKNTD TFAAVFSFLS GRLVHISEQA ALILNSKRGF LKSVHFVDLL APQDVRAFYA 

       190        200        210        220        230        240 
HTAPTQLPFW NNWTQRASQY ECAPAKPFFC RICGGGDREK RHYSPFRILP YLVHVHSSAQ 

       250        260        270        280        290        300 
PEPEPCCLTL VEKIHSGYEA PRIPVDKRIF TTTHTPGCVF LEVDERAVPL LGYLPQDLIG 

       310        320        330        340        350        360 
TSILTYLHPE DRPLMVAIHQ KVLKYAGHPP FEHSPVRFCT QNGEYVILDS SWSSFVNPWS 

       370        380        390        400        410        420 
RKVSFIIGRH KVRTSPLNED VFATRIKKAA SNDKDIAELQ EQIHKLLLQP VHASASSGYG 

       430        440        450        460        470        480 
SLGSSGSQEQ HVSITSSSES SGHCPEEGQH EQMTLQQVYA SVNKIKNVGQ QLYIESMARS 

       490        500        510        520        530        540 
SVKPVAETCV EPQGGDEQKD FSSSQTLKNK STTDTGSGGN LQQEQPSSSY QQMNCIDSVI 

       550        560        570        580        590        600 
RYLTSYSLPA LKRKCISCTN TSSSSEEAKP IPEVDSSQRD TEQLLDIRKQ ETTGPSTDIE 

       610        620        630        640        650        660 
GGAARTLSTA ALSVASGISQ CSCSSTSGHA PPLQSESVAV ACKPWALRTK ASHLAAGGFK 

       670        680        690        700        710        720 
HVGLTAAVLS AHTQKEEQNY VDRFREKILT SPYGCYLQQE SRNRAQYSCV QAGSTAKHSR 

       730        740        750        760        770        780 
CAGSERQKHK RKKLPAPVDT SSPGAHLCPH VTGLLPDEQH WGPSASPSPL GAGLAFPSAL 

       790        800        810        820        830        840 
VVPSQTPYLL PSFPLQDMAS QGVGVSAAWG AAAGCPPLSA GPQAVAAFPS AYVDTLMTIF 

       850        860        870        880        890        900 
LHNAPLFPLW PPSFSPYPSL GAAGSSELAP LVPAMAPNPE PTTSGHSQRR VEENWEAHSE 

       910        920        930        940        950        960 
ELPFISSRSS SPLQLNLLQE EMPAPSESAD AVRRGAGPDA KHHCVTGPSG SRSRHCTSGE 

       970        980        990       1000       1010       1020 
LATATAQQES AAASGSSASS IYFSSTDYAS EVSENRQRPQ DRQRDEALPG AAEESIWRMI 

      1030       1040       1050       1060       1070       1080 
ERTPECVLMT YQVPERGREE VLKQDLEKLQ SMEQQQPLFS PAQREELAKV RSWIHSHTAP 

      1090       1100       1110 
QEGHLQSCVA CEDRGSVGDT AEVLEQHPAE DTS 

« Hide

References

« Hide 'large scale' references
[1]"Three period homologs in mammals: differential light responses in the suprachiasmatic circadian clock and oscillating transcripts outside of brain."
Zylka M.J., Shearman L.P., Weaver D.R., Reppert S.M.
Neuron 20:1103-1110(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION.
Strain: C57BL/6.
Tissue: Brain.
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"mCRY1 and mCRY2 are essential components of the negative limb of the circadian clock feedback loop."
Kume K., Zylka M.J., Sriram S., Shearman L.P., Weaver D.R., Jin X., Maywood E.S., Hastings M.H., Reppert S.M.
Cell 98:193-205(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: HOMODIMERIZATION, INTERACTION WITH PER1; PER2; CRY1 AND CRY2, SUBCELLULAR LOCATION.
[4]"Nuclear export of mammalian PERIOD proteins."
Vielhaber E.L., Duricka D., Ullman K.S., Virshup D.M.
J. Biol. Chem. 276:45921-45927(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, NUCLEAR EXPORT SIGNAL.
[5]"Control of intracellular dynamics of mammalian period proteins by casein kinase I epsilon (CKIepsilon) and CKIdelta in cultured cells."
Akashi M., Tsuchiya Y., Yoshino T., Nishida E.
Mol. Cell. Biol. 22:1693-1703(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CSNK1D AND CSNK1E, PHOSPHORYLATION, UBIQUITINATION, SUBCELLULAR LOCATION.
[6]"Requirement of mammalian Timeless for circadian rhythmicity."
Barnes J.W., Tischkau S.A., Barnes J.A., Mitchell J.W., Burgoon P.W., Hickok J.R., Gillette M.U.
Science 302:439-442(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH TIMELESS, INDUCTION.
[7]"Direct association between mouse PERIOD and CKIepsilon is critical for a functioning circadian clock."
Lee C., Weaver D.R., Reppert S.M.
Mol. Cell. Biol. 24:584-594(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH PER1; CRY1 AND CRY2, PHOSPHORYLATION, SUBCELLULAR LOCATION, INDUCTION.
[8]"Unwinding the differences of the mammalian PERIOD clock proteins from crystal structure to cellular function."
Kucera N., Schmalen I., Hennig S., Ollinger R., Strauss H.M., Grudziecki A., Wieczorek C., Kramer A., Wolf E.
Proc. Natl. Acad. Sci. U.S.A. 109:3311-3316(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS) OF 108-411, FUNCTION IN HEME BINDING, SUBUNIT, MUTAGENESIS OF TRP-359 AND ILE-367.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF050182 mRNA. Translation: AAC40147.1.
AL607143 Genomic DNA. Translation: CAM24786.1.
PIRT14260.
RefSeqNP_035197.2. NM_011067.3.
UniGeneMm.121361.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
4DJ3X-ray2.50A/B108-411[»]
ProteinModelPortalO70361.
SMRO70361. Positions 40-484.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202113. 7 interactions.
STRING10090.ENSMUSP00000099493.

PTM databases

PhosphoSiteO70361.

Proteomic databases

PRIDEO70361.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000103204; ENSMUSP00000099493; ENSMUSG00000028957.
GeneID18628.
KEGGmmu:18628.
UCSCuc008vye.1. mouse.

Organism-specific databases

CTD8863.
MGIMGI:1277134. Per3.

Phylogenomic databases

eggNOGNOG269786.
GeneTreeENSGT00510000046467.
HOVERGENHBG008167.
InParanoidA2A894.
KOK02633.
OMAFKHVGLT.
OrthoDBEOG7S7SD0.
TreeFamTF318445.

Gene expression databases

ArrayExpressO70361.
BgeeO70361.
CleanExMM_PER3.
GenevestigatorO70361.

Family and domain databases

InterProIPR001610. PAC.
IPR000014. PAS.
IPR022728. Period_circadian-like_C.
[Graphical view]
PfamPF12114. Period_C. 1 hit.
[Graphical view]
SMARTSM00086. PAC. 1 hit.
SM00091. PAS. 2 hits.
[Graphical view]
SUPFAMSSF55785. SSF55785. 1 hit.
PROSITEPS50112. PAS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294584.
PROO70361.
SOURCESearch...

Entry information

Entry namePER3_MOUSE
AccessionPrimary (citable) accession number: O70361
Secondary accession number(s): A2A894
Entry history
Integrated into UniProtKB/Swiss-Prot: July 15, 1999
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot