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Protein

3-hydroxyacyl-CoA dehydrogenase type-2

Gene

Hsd17b10

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. Catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids (By similarity).By similarity

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+ = 2-methylacetoacetyl-CoA + NADH.
Testosterone + NAD(P)+ = androst-4-ene-3,17-dione + NAD(P)H.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei155Substrate1 Publication1
Active sitei168Proton acceptor1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 37NADBy similarityAdd BLAST26

GO - Molecular functioni

  • 3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity Source: UniProtKB-EC
  • 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-EC
  • acetoacetyl-CoA reductase activity Source: RGD
  • beta-amyloid binding Source: RGD
  • estradiol 17-beta-dehydrogenase activity Source: RGD
  • estrogen receptor binding Source: RGD
  • identical protein binding Source: RGD
  • NAD binding Source: RGD
  • steroid binding Source: RGD
  • testosterone dehydrogenase [NAD(P)] activity Source: UniProtKB-EC

GO - Biological processi

  • cell aging Source: RGD
  • Leydig cell differentiation Source: RGD
  • protein homotetramerization Source: RGD
  • tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKO70351.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyacyl-CoA dehydrogenase type-2 (EC:1.1.1.35)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 10 (EC:1.1.1.51)
Short name:
17-beta-HSD 10
3-hydroxy-2-methylbutyryl-CoA dehydrogenase (EC:1.1.1.178)
3-hydroxyacyl-CoA dehydrogenase type II
Endoplasmic reticulum-associated amyloid beta-peptide-binding protein
Mitochondrial ribonuclease P protein 2
Short name:
Mitochondrial RNase P protein 2
Type II HADH
Gene namesi
Name:Hsd17b10
Synonyms:Erab, Hadh2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi69231. Hsd17b10.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000548122 – 2613-hydroxyacyl-CoA dehydrogenase type-2Add BLAST260

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei53N6-acetyllysine; alternateBy similarity1
Modified residuei53N6-succinyllysine; alternateBy similarity1
Modified residuei69N6-acetyllysineBy similarity1
Modified residuei99N6-acetyllysineBy similarity1
Modified residuei105N6-acetyllysineBy similarity1
Modified residuei212N6-acetyllysine; alternateBy similarity1
Modified residuei212N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO70351.
PRIDEiO70351.

PTM databases

iPTMnetiO70351.
PhosphoSitePlusiO70351.

Interactioni

Subunit structurei

Homotetramer. Interacts with MRPP1/TRMT10C and MRPP3/KIAA0391 (By similarity).By similarity

GO - Molecular functioni

  • estrogen receptor binding Source: RGD
  • identical protein binding Source: RGD

Protein-protein interaction databases

IntActiO70351. 1 interactor.
MINTiMINT-4568352.
STRINGi10116.ENSRNOP00000043608.

Structurei

Secondary structure

1261
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 16Combined sources5
Turni17 – 19Combined sources3
Helixi21 – 32Combined sources12
Beta strandi36 – 41Combined sources6
Helixi47 – 54Combined sources8
Beta strandi58 – 62Combined sources5
Helixi68 – 82Combined sources15
Beta strandi87 – 90Combined sources4
Beta strandi100 – 102Combined sources3
Turni103 – 106Combined sources4
Helixi111 – 121Combined sources11
Helixi123 – 137Combined sources15
Beta strandi148 – 153Combined sources6
Helixi157 – 160Combined sources4
Helixi166 – 186Combined sources21
Helixi187 – 189Combined sources3
Beta strandi191 – 198Combined sources8
Beta strandi201 – 203Combined sources3
Turni204 – 206Combined sources3
Helixi214 – 217Combined sources4
Helixi218 – 220Combined sources3
Beta strandi222 – 224Combined sources3
Helixi230 – 242Combined sources13
Beta strandi250 – 254Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3SX-ray2.00A/B/C/D2-261[»]
1E3WX-ray2.00A/B/C/D2-261[»]
1E6WX-ray1.70A/B/C/D2-261[»]
ProteinModelPortaliO70351.
SMRiO70351.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiO70351.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1199. Eukaryota.
ENOG410XNNW. LUCA.
HOVERGENiHBG002145.
InParanoidiO70351.
KOiK08683.
PhylomeDBiO70351.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70351-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAVRSVKG LVAVITGGAS GLGLSTAKRL VGQGATAVLL DVPNSEGETE
60 70 80 90 100
AKKLGGNCIF APANVTSEKE VQAALTLAKE KFGRIDVAVN CAGIAVAIKT
110 120 130 140 150
YHEKKNQVHT LEDFQRVINV NLIGTFNVIR LVAGVMGQNE PDQGGQRGVI
160 170 180 190 200
INTASVAAFE GQVGQAAYSA SKGGIVGMTL PIARDLAPIG IRVVTIAPGL
210 220 230 240 250
FATPLLTTLP DKVRNFLASQ VPFPSRLGDP AEYAHLVQMV IENPFLNGEV
260
IRLDGAIRMQ P
Length:261
Mass (Da):27,246
Last modified:January 23, 2007 - v3
Checksum:i117FD723B11EA227
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5V → C in AAF14853 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049878 mRNA. Translation: AAC05747.1.
AF069770 mRNA. Translation: AAF14853.1.
RefSeqiNP_113870.1. NM_031682.1.
UniGeneiRn.2700.

Genome annotation databases

GeneIDi63864.
KEGGirno:63864.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF049878 mRNA. Translation: AAC05747.1.
AF069770 mRNA. Translation: AAF14853.1.
RefSeqiNP_113870.1. NM_031682.1.
UniGeneiRn.2700.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E3SX-ray2.00A/B/C/D2-261[»]
1E3WX-ray2.00A/B/C/D2-261[»]
1E6WX-ray1.70A/B/C/D2-261[»]
ProteinModelPortaliO70351.
SMRiO70351.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiO70351. 1 interactor.
MINTiMINT-4568352.
STRINGi10116.ENSRNOP00000043608.

PTM databases

iPTMnetiO70351.
PhosphoSitePlusiO70351.

Proteomic databases

PaxDbiO70351.
PRIDEiO70351.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi63864.
KEGGirno:63864.

Organism-specific databases

CTDi3028.
RGDi69231. Hsd17b10.

Phylogenomic databases

eggNOGiKOG1199. Eukaryota.
ENOG410XNNW. LUCA.
HOVERGENiHBG002145.
InParanoidiO70351.
KOiK08683.
PhylomeDBiO70351.

Enzyme and pathway databases

SABIO-RKO70351.

Miscellaneous databases

EvolutionaryTraceiO70351.
PROiO70351.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
IPR002347. SDR_fam.
[Graphical view]
PANTHERiPTHR24322. PTHR24322. 2 hits.
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
SUPFAMiSSF51735. SSF51735. 1 hit.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHCD2_RAT
AccessioniPrimary (citable) accession number: O70351
Secondary accession number(s): Q9QYD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.