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O70351

- HCD2_RAT

UniProt

O70351 - HCD2_RAT

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Protein

3-hydroxyacyl-CoA dehydrogenase type-2

Gene
Hsd17b10, Erab, Hadh2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Functions in mitochondrial tRNA maturation. Part of mitochondrial ribonuclease P, an enzyme composed of MRPP1/TRMT10C, MRPP2/HSD17B10 and MRPP3/KIAA0391, which cleaves tRNA molecules in their 5'-ends. Catalyzes the beta-oxidation at position 17 of androgens and estrogens and has 3-alpha-hydroxysteroid dehydrogenase activity with androsterone. Catalyzes the third step in the beta-oxidation of fatty acids. Carries out oxidative conversions of 7-alpha-OH and 7-beta-OH bile acids. Also exhibits 20-beta-OH and 21-OH dehydrogenase activities with C21 steroids By similarity.

Catalytic activityi

(S)-3-hydroxyacyl-CoA + NAD+ = 3-oxoacyl-CoA + NADH.
(2S,3S)-3-hydroxy-2-methylbutanoyl-CoA + NAD+ = 2-methylacetoacetyl-CoA + NADH.
Testosterone + NAD(P)+ = androst-4-ene-3,17-dione + NAD(P)H.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei155 – 1551Substrate
Active sitei168 – 1681Proton acceptor

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 3726NAD By similarityAdd
BLAST

GO - Molecular functioni

  1. 3-hydroxy-2-methylbutyryl-CoA dehydrogenase activity Source: UniProtKB-EC
  2. 3-hydroxyacyl-CoA dehydrogenase activity Source: UniProtKB-EC
  3. acetoacetyl-CoA reductase activity Source: RGD
  4. beta-amyloid binding Source: RGD
  5. estradiol 17-beta-dehydrogenase activity Source: RGD
  6. estrogen receptor binding Source: RGD
  7. identical protein binding Source: RGD
  8. NAD binding Source: RGD
  9. steroid binding Source: RGD
  10. testosterone dehydrogenase [NAD(P)] activity Source: UniProtKB-EC

GO - Biological processi

  1. cell aging Source: RGD
  2. Leydig cell differentiation Source: RGD
  3. protein homotetramerization Source: RGD
  4. tRNA processing Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

NAD

Enzyme and pathway databases

SABIO-RKO70351.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxyacyl-CoA dehydrogenase type-2 (EC:1.1.1.35)
Alternative name(s):
17-beta-hydroxysteroid dehydrogenase 10 (EC:1.1.1.51)
Short name:
17-beta-HSD 10
3-hydroxy-2-methylbutyryl-CoA dehydrogenase (EC:1.1.1.178)
3-hydroxyacyl-CoA dehydrogenase type II
Endoplasmic reticulum-associated amyloid beta-peptide-binding protein
Mitochondrial ribonuclease P protein 2
Short name:
Mitochondrial RNase P protein 2
Type II HADH
Gene namesi
Name:Hsd17b10
Synonyms:Erab, Hadh2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi69231. Hsd17b10.

Subcellular locationi

Mitochondrion By similarity

GO - Cellular componenti

  1. mitochondrion Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 2612603-hydroxyacyl-CoA dehydrogenase type-2PRO_0000054812Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanine By similarity
Modified residuei53 – 531N6-acetyllysine; alternate By similarity
Modified residuei53 – 531N6-succinyllysine; alternate By similarity
Modified residuei69 – 691N6-acetyllysine By similarity
Modified residuei99 – 991N6-acetyllysine By similarity
Modified residuei105 – 1051N6-acetyllysine By similarity
Modified residuei212 – 2121N6-acetyllysine; alternate By similarity
Modified residuei212 – 2121N6-succinyllysine; alternate By similarity

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiO70351.
PRIDEiO70351.

Expressioni

Gene expression databases

GenevestigatoriO70351.

Interactioni

Subunit structurei

Homotetramer. Interacts with MRPP1/TRMT10C and MRPP3/KIAA0391 By similarity.

Protein-protein interaction databases

IntActiO70351. 1 interaction.
MINTiMINT-4568352.

Structurei

Secondary structure

1
261
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi12 – 165
Turni17 – 193
Helixi21 – 3212
Beta strandi36 – 416
Helixi47 – 548
Beta strandi58 – 625
Helixi68 – 8215
Beta strandi87 – 904
Beta strandi100 – 1023
Turni103 – 1064
Helixi111 – 12111
Helixi123 – 13715
Beta strandi148 – 1536
Helixi157 – 1604
Helixi166 – 18621
Helixi187 – 1893
Beta strandi191 – 1988
Beta strandi201 – 2033
Turni204 – 2063
Helixi214 – 2174
Helixi218 – 2203
Beta strandi222 – 2243
Helixi230 – 24213
Beta strandi250 – 2545

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E3SX-ray2.00A/B/C/D2-261[»]
1E3WX-ray2.00A/B/C/D2-261[»]
1E6WX-ray1.70A/B/C/D2-261[»]
ProteinModelPortaliO70351.
SMRiO70351. Positions 7-261.

Miscellaneous databases

EvolutionaryTraceiO70351.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1028.
HOVERGENiHBG002145.
InParanoidiO70351.
KOiK08683.
PhylomeDBiO70351.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view]
PfamiPF00106. adh_short. 1 hit.
[Graphical view]
PRINTSiPR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEiPS00061. ADH_SHORT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70351-1 [UniParc]FASTAAdd to Basket

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MAAAVRSVKG LVAVITGGAS GLGLSTAKRL VGQGATAVLL DVPNSEGETE    50
AKKLGGNCIF APANVTSEKE VQAALTLAKE KFGRIDVAVN CAGIAVAIKT 100
YHEKKNQVHT LEDFQRVINV NLIGTFNVIR LVAGVMGQNE PDQGGQRGVI 150
INTASVAAFE GQVGQAAYSA SKGGIVGMTL PIARDLAPIG IRVVTIAPGL 200
FATPLLTTLP DKVRNFLASQ VPFPSRLGDP AEYAHLVQMV IENPFLNGEV 250
IRLDGAIRMQ P 261
Length:261
Mass (Da):27,246
Last modified:January 23, 2007 - v3
Checksum:i117FD723B11EA227
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51V → C in AAF14853. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF049878 mRNA. Translation: AAC05747.1.
AF069770 mRNA. Translation: AAF14853.1.
RefSeqiNP_113870.1. NM_031682.1.
UniGeneiRn.2700.

Genome annotation databases

GeneIDi63864.
KEGGirno:63864.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF049878 mRNA. Translation: AAC05747.1 .
AF069770 mRNA. Translation: AAF14853.1 .
RefSeqi NP_113870.1. NM_031682.1.
UniGenei Rn.2700.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1E3S X-ray 2.00 A/B/C/D 2-261 [» ]
1E3W X-ray 2.00 A/B/C/D 2-261 [» ]
1E6W X-ray 1.70 A/B/C/D 2-261 [» ]
ProteinModelPortali O70351.
SMRi O70351. Positions 7-261.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi O70351. 1 interaction.
MINTi MINT-4568352.

Proteomic databases

PaxDbi O70351.
PRIDEi O70351.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 63864.
KEGGi rno:63864.

Organism-specific databases

CTDi 3028.
RGDi 69231. Hsd17b10.

Phylogenomic databases

eggNOGi COG1028.
HOVERGENi HBG002145.
InParanoidi O70351.
KOi K08683.
PhylomeDBi O70351.

Enzyme and pathway databases

SABIO-RK O70351.

Miscellaneous databases

EvolutionaryTracei O70351.
NextBioi 612472.
PROi O70351.

Gene expression databases

Genevestigatori O70351.

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR002198. DH_sc/Rdtase_SDR.
IPR002347. Glc/ribitol_DH.
IPR016040. NAD(P)-bd_dom.
IPR020904. Sc_DH/Rdtase_CS.
[Graphical view ]
Pfami PF00106. adh_short. 1 hit.
[Graphical view ]
PRINTSi PR00081. GDHRDH.
PR00080. SDRFAMILY.
PROSITEi PS00061. ADH_SHORT. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Rattus norvegicus amyloid beta-peptide binding protein (ERAB) mRNA."
    Gunn-Moore F.J., Tavare J.M.
    Submitted (FEB-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Molecular cloning and characterization of the cDNA of rat brain short chain L-3-hydroxyacyl-CoA dehydrogenase."
    Yang S.-Y., He X.-Y.
    Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Brain.
  3. Lubec G., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 117-130 AND 193-212, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.
  4. "Recognition of structurally diverse substrates by type II 3-hydroxyacyl-CoA dehydrogenase (HADH II)/amyloid-beta binding alcohol dehydrogenase (ABAD)."
    Powell A.J., Read J.A., Banfield M.J., Gunn-Moore F., Yan S.D., Lustbader J., Stern A.R., Stern D.M., Brady R.L.
    J. Mol. Biol. 303:311-327(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) IN COMPLEX WITH NAD AND SUBSTRATE.
    Tissue: Brain.

Entry informationi

Entry nameiHCD2_RAT
AccessioniPrimary (citable) accession number: O70351
Secondary accession number(s): Q9QYD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 30, 2000
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 113 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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