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Protein

Ribonuclease H1

Gene

Rnaseh1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Endonuclease that specifically degrades the RNA of RNA-DNA hybrids. Plays a role in RNA polymerase II (RNAp II) transcription termination by degrading R-loop RNA-DNA hybrid formation at G-rich pause sites located downstream of the poly(A) site and behind the elongating RNAp II.By similarity

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphomonoester.PROSITE-ProRule annotation

Cofactori

Mg2+By similarityNote: Binds 1 Mg2+ ion per subunit. May bind a second metal ion at a regulatory site, or after substrate binding.By similarity

Enzyme regulationi

In the presence of magnesium, manganese is inhibitory.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi144 – 1441Magnesium 1PROSITE-ProRule annotation
Metal bindingi144 – 1441Magnesium 2PROSITE-ProRule annotation
Metal bindingi185 – 1851Magnesium 1PROSITE-ProRule annotation
Metal bindingi209 – 2091Magnesium 1PROSITE-ProRule annotation
Metal bindingi273 – 2731Magnesium 2PROSITE-ProRule annotation

GO - Molecular functioni

  • magnesium ion binding Source: InterPro
  • nucleic acid binding Source: InterPro
  • protein homodimerization activity Source: MGI
  • RNA-DNA hybrid ribonuclease activity Source: MGI

GO - Biological processi

  • DNA replication, removal of RNA primer Source: GO_Central
  • mitochondrial DNA replication Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Ligandi

Magnesium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Ribonuclease H1 (EC:3.1.26.4)
Short name:
RNase H1
Gene namesi
Name:Rnaseh1
Synonyms:Rnh1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1335073. Rnaseh1.

Subcellular locationi

GO - Cellular componenti

  • mitochondrion Source: MGI
  • nucleus Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 285285Ribonuclease H1PRO_0000195434Add
BLAST

Proteomic databases

EPDiO70338.
MaxQBiO70338.
PaxDbiO70338.
PeptideAtlasiO70338.
PRIDEiO70338.

PTM databases

iPTMnetiO70338.
PhosphoSiteiO70338.

Expressioni

Gene expression databases

CleanExiMM_RNASEH1.
MM_RNH1.

Interactioni

Subunit structurei

Monomer.By similarity

GO - Molecular functioni

  • protein homodimerization activity Source: MGI

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020959.

Structurei

3D structure databases

ProteinModelPortaliO70338.
SMRiO70338. Positions 27-72, 139-281.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini135 – 281147RNase HPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the RNase H family.Curated
Contains 1 RNase H domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3752. Eukaryota.
COG0328. LUCA.
HOGENOMiHOG000040465.
HOVERGENiHBG002135.
InParanoidiO70338.
PhylomeDBiO70338.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.970.10. 1 hit.
InterProiIPR009027. Ribosomal_L9/RNase_H1_N.
IPR017067. RNase_H1_euk.
IPR011320. RNase_H1_N.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF01693. Cauli_VI. 1 hit.
PF00075. RNase_H. 1 hit.
[Graphical view]
PIRSFiPIRSF036852. Ribonuclease_H1_euk. 1 hit.
SUPFAMiSSF53098. SSF53098. 1 hit.
SSF55658. SSF55658. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

O70338-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRWLLPLSRT VTLAVVRLRR GICGLGMFYA VRRGRRTGVF LSWSECKAQV
60 70 80 90 100
DRFPAARFKK FATEDEAWAF VRSSSSPDGS KGQESAHEQK SQAKTSKRPR
110 120 130 140 150
EPLGEGEELP EPGPKHTRQD TEPAAVVSKD TFSYMGESVI VYTDGCCSSN
160 170 180 190 200
GRKRARAGIG VYWGPGHPLN VGIRLPGRQT NQRAEIHAAC KAIMQAKAQN
210 220 230 240 250
ISKLVLYTDS MFTINGITNW VQGWKKNGWR TSTGKDVINK EDFMELDELT
260 270 280
QGMDIQWMHI PGHSGFVGNE EADRLAREGA KQSED
Length:285
Mass (Da):31,805
Last modified:August 1, 1998 - v1
Checksum:i92B09F8EFAFA822F
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti93 – 931A → V in AAH19411 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048993 mRNA. Translation: AAC78562.1.
BC019411 mRNA. Translation: AAH19411.1.
CCDSiCCDS25852.1.
UniGeneiMm.10152.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048993 mRNA. Translation: AAC78562.1.
BC019411 mRNA. Translation: AAH19411.1.
CCDSiCCDS25852.1.
UniGeneiMm.10152.

3D structure databases

ProteinModelPortaliO70338.
SMRiO70338. Positions 27-72, 139-281.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000020959.

PTM databases

iPTMnetiO70338.
PhosphoSiteiO70338.

Proteomic databases

EPDiO70338.
MaxQBiO70338.
PaxDbiO70338.
PeptideAtlasiO70338.
PRIDEiO70338.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1335073. Rnaseh1.

Phylogenomic databases

eggNOGiKOG3752. Eukaryota.
COG0328. LUCA.
HOGENOMiHOG000040465.
HOVERGENiHBG002135.
InParanoidiO70338.
PhylomeDBiO70338.

Miscellaneous databases

ChiTaRSiRnaseh1. mouse.
PROiO70338.
SOURCEiSearch...

Gene expression databases

CleanExiMM_RNASEH1.
MM_RNH1.

Family and domain databases

Gene3Di3.30.420.10. 1 hit.
3.40.970.10. 1 hit.
InterProiIPR009027. Ribosomal_L9/RNase_H1_N.
IPR017067. RNase_H1_euk.
IPR011320. RNase_H1_N.
IPR012337. RNaseH-like_dom.
IPR002156. RNaseH_domain.
[Graphical view]
PfamiPF01693. Cauli_VI. 1 hit.
PF00075. RNase_H. 1 hit.
[Graphical view]
PIRSFiPIRSF036852. Ribonuclease_H1_euk. 1 hit.
SUPFAMiSSF53098. SSF53098. 1 hit.
SSF55658. SSF55658. 1 hit.
PROSITEiPS50879. RNASE_H. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRNH1_MOUSE
AccessioniPrimary (citable) accession number: O70338
Secondary accession number(s): Q8VCR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: August 1, 1998
Last modified: September 7, 2016
This is version 119 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.