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Protein

Bcl-2-interacting killer

Gene

Bik

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Accelerates programmed cell death. Binding to the apoptosis repressors Bcl-X(L), BHRF1 or Bcl-2 suppresses this death-promoting activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei143 – 1442Cleavage; by RHBDL4/RHBDD1By similarity

GO - Biological processi

  1. apoptotic mitochondrial changes Source: MGI
  2. male gonad development Source: MGI
  3. positive regulation of protein homooligomerization Source: MGI
  4. positive regulation of release of cytochrome c from mitochondria Source: MGI
  5. spermatogenesis Source: MGI
Complete GO annotation...

Keywords - Biological processi

Apoptosis

Names & Taxonomyi

Protein namesi
Recommended name:
Bcl-2-interacting killer
Alternative name(s):
Apoptosis inducer NBK
Bik-like killer protein
Gene namesi
Name:Bik
Synonyms:Biklk, Blk, Nbk
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1206591. Bik.

Subcellular locationi

Endomembrane system By similarity; Single-pass membrane protein By similarity. Mitochondrion membrane 1 Publication; Single-pass membrane protein 1 Publication
Note: Around the nuclear envelope, and in cytoplasmic membranes.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei127 – 14721HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. endomembrane system Source: UniProtKB-SubCell
  2. integral component of membrane Source: UniProtKB-KW
  3. mitochondrial envelope Source: MGI
  4. mitochondrial membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 150150Bcl-2-interacting killerPRO_0000419202Add
BLAST

Post-translational modificationi

Proteolytically cleaved by RHBDL4/RHBDD1. RHBDL4/RHBDD1-induced cleavage is a necessary step prior its degradation by the proteosome-dependent mechanism (By similarity).By similarity

Expressioni

Tissue specificityi

Expressed in testis, kidney, liver, lung and heart.1 Publication

Gene expression databases

BgeeiO70337.
ExpressionAtlasiO70337. baseline and differential.
GenevestigatoriO70337.

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000016902.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni127 – 14822Leucine-zipperSequence AnalysisAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi51 – 6515BH3By similarityAdd
BLAST

Domaini

Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.By similarity

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG39366.
GeneTreeiENSGT00530000064453.
HOVERGENiHBG001515.
InParanoidiO70337.
KOiK18452.
OMAiLECMEGS.
OrthoDBiEOG7NKKN5.
TreeFamiTF338339.

Family and domain databases

InterProiIPR024579. Bcl2-int_killer.
[Graphical view]
PANTHERiPTHR15018. PTHR15018. 1 hit.
PfamiPF12201. bcl-2I13. 1 hit.
[Graphical view]
ProDomiPD032623. PD032623. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

O70337-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEARLMARD VIKTVPHDQV PQPPVASETP SMKEPVRDVD LMECVEGRNQ
60 70 80 90 100
VALRLACIGD EMDLCLRSPR LVQLPGIAIH RLAVTYSRTG VRGIFRSLIR
110 120 130 140 150
SLTNLRENIW SWRVLTPGAW VSPDQDPGQL FPMVLLVFLL LGGAWYLQLQ
Length:150
Mass (Da):16,901
Last modified:August 1, 1998 - v1
Checksum:i120CB6BAA4612788
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048838 mRNA. Translation: AAC40079.1.
AL583889 Genomic DNA. No translation available.
CH467427 Genomic DNA. Translation: EDL01409.1.
CCDSiCCDS27703.1.
RefSeqiNP_031572.1. NM_007546.2.
XP_006520418.1. XM_006520355.2.
XP_006520419.1. XM_006520356.2.
XP_006520420.1. XM_006520357.2.
UniGeneiMm.267006.

Genome annotation databases

EnsembliENSMUST00000016902; ENSMUSP00000016902; ENSMUSG00000016758.
GeneIDi12124.
KEGGimmu:12124.
UCSCiuc007xbd.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF048838 mRNA. Translation: AAC40079.1.
AL583889 Genomic DNA. No translation available.
CH467427 Genomic DNA. Translation: EDL01409.1.
CCDSiCCDS27703.1.
RefSeqiNP_031572.1. NM_007546.2.
XP_006520418.1. XM_006520355.2.
XP_006520419.1. XM_006520356.2.
XP_006520420.1. XM_006520357.2.
UniGeneiMm.267006.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000016902.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000016902; ENSMUSP00000016902; ENSMUSG00000016758.
GeneIDi12124.
KEGGimmu:12124.
UCSCiuc007xbd.1. mouse.

Organism-specific databases

CTDi638.
MGIiMGI:1206591. Bik.

Phylogenomic databases

eggNOGiNOG39366.
GeneTreeiENSGT00530000064453.
HOVERGENiHBG001515.
InParanoidiO70337.
KOiK18452.
OMAiLECMEGS.
OrthoDBiEOG7NKKN5.
TreeFamiTF338339.

Miscellaneous databases

ChiTaRSiBik. mouse.
NextBioi280447.
PROiO70337.
SOURCEiSearch...

Gene expression databases

BgeeiO70337.
ExpressionAtlasiO70337. baseline and differential.
GenevestigatoriO70337.

Family and domain databases

InterProiIPR024579. Bcl2-int_killer.
[Graphical view]
PANTHERiPTHR15018. PTHR15018. 1 hit.
PfamiPF12201. bcl-2I13. 1 hit.
[Graphical view]
ProDomiPD032623. PD032623. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Blk, a BH3-containing mouse protein that interacts with Bcl-2 and Bcl-xL, is a potent death agonist."
    Hegde R., Srinivasula S.M., Ahmad M., Fernandes-Alnemri T., Alnemri E.S.
    J. Biol. Chem. 273:7783-7786(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN APOPTOSIS, SUBCELLULAR LOCATION, TISSUE SPECIFICITY.
    Tissue: Embryo.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiBIK_MOUSE
AccessioniPrimary (citable) accession number: O70337
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 3, 2012
Last sequence update: August 1, 1998
Last modified: April 1, 2015
This is version 76 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.