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Protein

Monocarboxylate transporter 8

Gene

Slc16a2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Very active and specific thyroid hormone transporter. Stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. Does not transport Leu, Phe, Trp or Tyr.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Symport, Transport

Enzyme and pathway databases

ReactomeiR-MMU-879518. Transport of organic anions.

Protein family/group databases

TCDBi2.A.1.13.3. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Monocarboxylate transporter 8
Short name:
MCT 8
Alternative name(s):
Solute carrier family 16 member 2
X-linked PEST-containing transporter
Gene namesi
Name:Slc16a2
Synonyms:Mct8, Xpct
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome X

Organism-specific databases

MGIiMGI:1203732. Slc16a2.

Subcellular locationi

  • Cell membrane By similarity; Multi-pass membrane protein By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini2 – 102CytoplasmicSequence analysisAdd BLAST101
Transmembranei103 – 123HelicalSequence analysisAdd BLAST21
Topological domaini124 – 149ExtracellularSequence analysisAdd BLAST26
Transmembranei150 – 170HelicalSequence analysisAdd BLAST21
Topological domaini171 – 177CytoplasmicSequence analysis7
Transmembranei178 – 198HelicalSequence analysisAdd BLAST21
Topological domaini199 – 206ExtracellularSequence analysis8
Transmembranei207 – 227HelicalSequence analysisAdd BLAST21
Topological domaini228 – 235CytoplasmicSequence analysis8
Transmembranei236 – 256HelicalSequence analysisAdd BLAST21
Topological domaini257 – 264ExtracellularSequence analysis8
Transmembranei265 – 285HelicalSequence analysisAdd BLAST21
Topological domaini286 – 328CytoplasmicSequence analysisAdd BLAST43
Transmembranei329 – 349HelicalSequence analysisAdd BLAST21
Topological domaini350 – 362ExtracellularSequence analysisAdd BLAST13
Transmembranei363 – 383HelicalSequence analysisAdd BLAST21
Topological domaini384 – 392CytoplasmicSequence analysis9
Transmembranei393 – 413HelicalSequence analysisAdd BLAST21
Topological domaini414 – 415ExtracellularSequence analysis2
Transmembranei416 – 436HelicalSequence analysisAdd BLAST21
Topological domaini437 – 453CytoplasmicSequence analysisAdd BLAST17
Transmembranei454 – 474HelicalSequence analysisAdd BLAST21
Topological domaini475 – 483ExtracellularSequence analysis9
Transmembranei484 – 504HelicalSequence analysisAdd BLAST21
Topological domaini505 – 545CytoplasmicSequence analysisAdd BLAST41

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002114022 – 545Monocarboxylate transporter 8Add BLAST544

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei540PhosphothreonineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiO70324.
PeptideAtlasiO70324.
PRIDEiO70324.

PTM databases

iPTMnetiO70324.
PhosphoSitePlusiO70324.

Expressioni

Tissue specificityi

Highly expressed in liver and kidney.

Gene expression databases

BgeeiENSMUSG00000033965.
ExpressionAtlasiO70324. baseline and differential.
GenevisibleiO70324. MM.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037629.

Structurei

3D structure databases

ProteinModelPortaliO70324.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati29 – 501Add BLAST22
Repeati51 – 722Add BLAST22

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni29 – 722 X 22 AA approximate tandem repeatsAdd BLAST44

Sequence similaritiesi

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IMVZ. Eukaryota.
ENOG410Z4JM. LUCA.
GeneTreeiENSGT00740000115479.
HOGENOMiHOG000046644.
HOVERGENiHBG006387.
InParanoidiO70324.
KOiK08231.
OMAiIKQTWVL.
OrthoDBiEOG091G0449.
PhylomeDBiO70324.
TreeFamiTF313792.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR030761. MCT8.
IPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR11360:SF123. PTHR11360:SF123. 1 hit.
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALPSPASEE AEGPCQEANQ EYQEPVCSPV PEPEPEPEPE PEPDPEPVPV
60 70 80 90 100
PPPEPQPEPE PQPLPDPAPL PELGFEAEPV QEPEPTPTVE TRGTARGFQP
110 120 130 140 150
PEGGFGWIVV FAATWCNGSI FGIHNSVGIL YSMLLEEEKE KNRQVEFQAA
160 170 180 190 200
WVGALAMGMI FFCSPIVSIF TDRLGCRITA TTGAAVAFIG LHTSSFTSSL
210 220 230 240 250
SLRYFTYGIL FGCGCSFAFQ PSLVILGHYF QRRLGLANGV VSAGSSIFSM
260 270 280 290 300
SFPFLIKMLG DKIKLAQTFQ VLSTFMFVLT LLSLTYRPLL PSSQDTPSKR
310 320 330 340 350
GAHTLRQRFL VQFRKYFNMR VFRQRTYRIW AFGIAAAALG YFVPYVHLMK
360 370 380 390 400
YVEDKFKEIK ETWVLLVCIG ATSGLGRLVS GHISDSIPGL KKIYLQVLSF
410 420 430 440 450
LLLGLMSMMI PLCRDFGGLI VVCLFLGLCD GFFITIMAPI AFELVGPMQA
460 470 480 490 500
SQAIGYLLGM MALPMIAGPP IAGLLRNCFG DYHVAFYFAG VPPIIGAVIL
510 520 530 540
FFVPLMHQRM FKKEQRDSSK DKMLSHDPDP NGELLPGSPT PEEPI
Length:545
Mass (Da):60,025
Last modified:September 5, 2006 - v2
Checksum:iACCC7EC6B902A6DE
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti62Q → QPLPDPAPLPELGFEAEPEP Q (PubMed:9545634).Curated1
Sequence conflicti227G → D in AAC40078 (PubMed:9545634).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045692 mRNA. Translation: AAC40078.1.
AJ421478 Genomic DNA. Translation: CAD33931.1.
BC080678 mRNA. Translation: AAH80678.1.
CCDSiCCDS30330.1.
RefSeqiNP_033223.2. NM_009197.2.
UniGeneiMm.388973.

Genome annotation databases

EnsembliENSMUST00000042664; ENSMUSP00000037629; ENSMUSG00000033965.
GeneIDi20502.
KEGGimmu:20502.
UCSCiuc009tzy.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF045692 mRNA. Translation: AAC40078.1.
AJ421478 Genomic DNA. Translation: CAD33931.1.
BC080678 mRNA. Translation: AAH80678.1.
CCDSiCCDS30330.1.
RefSeqiNP_033223.2. NM_009197.2.
UniGeneiMm.388973.

3D structure databases

ProteinModelPortaliO70324.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000037629.

Protein family/group databases

TCDBi2.A.1.13.3. the major facilitator superfamily (mfs).

PTM databases

iPTMnetiO70324.
PhosphoSitePlusiO70324.

Proteomic databases

PaxDbiO70324.
PeptideAtlasiO70324.
PRIDEiO70324.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000042664; ENSMUSP00000037629; ENSMUSG00000033965.
GeneIDi20502.
KEGGimmu:20502.
UCSCiuc009tzy.1. mouse.

Organism-specific databases

CTDi6567.
MGIiMGI:1203732. Slc16a2.

Phylogenomic databases

eggNOGiENOG410IMVZ. Eukaryota.
ENOG410Z4JM. LUCA.
GeneTreeiENSGT00740000115479.
HOGENOMiHOG000046644.
HOVERGENiHBG006387.
InParanoidiO70324.
KOiK08231.
OMAiIKQTWVL.
OrthoDBiEOG091G0449.
PhylomeDBiO70324.
TreeFamiTF313792.

Enzyme and pathway databases

ReactomeiR-MMU-879518. Transport of organic anions.

Miscellaneous databases

ChiTaRSiSlc16a2. mouse.
PROiO70324.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000033965.
ExpressionAtlasiO70324. baseline and differential.
GenevisibleiO70324. MM.

Family and domain databases

CDDicd06174. MFS. 1 hit.
InterProiIPR030761. MCT8.
IPR011701. MFS.
IPR020846. MFS_dom.
[Graphical view]
PANTHERiPTHR11360:SF123. PTHR11360:SF123. 1 hit.
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMOT8_MOUSE
AccessioniPrimary (citable) accession number: O70324
Secondary accession number(s): Q8K3S9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: September 5, 2006
Last modified: November 30, 2016
This is version 131 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.