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O70324 (MOT8_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Monocarboxylate transporter 8

Short name=MCT 8
Alternative name(s):
Solute carrier family 16 member 2
X-linked PEST-containing transporter
Gene names
Name:Slc16a2
Synonyms:Mct8, Xpct
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length545 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Very active and specific thyroid hormone transporter. Stimulates cellular uptake of thyroxine (T4), triiodothyronine (T3), reverse triiodothyronine (rT3) and diidothyronine. Does not transport Leu, Phe, Trp or Tyr By similarity.

Subunit structure

Homodimer By similarity.

Subcellular location

Cell membrane; Multi-pass membrane protein By similarity.

Tissue specificity

Highly expressed in liver and kidney.

Sequence similarities

Belongs to the major facilitator superfamily. Monocarboxylate porter (TC 2.A.1.13) family. [View classification]

Ontologies

Keywords
   Biological processSymport
Transport
   Cellular componentCell membrane
Membrane
   DomainRepeat
Transmembrane
Transmembrane helix
   PTMAcetylation
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral component of membrane

Inferred from electronic annotation. Source: UniProtKB-KW

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionsymporter activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 545544Monocarboxylate transporter 8
PRO_0000211402

Regions

Topological domain2 – 102101Cytoplasmic Potential
Transmembrane103 – 12321Helical; Potential
Topological domain124 – 14926Extracellular Potential
Transmembrane150 – 17021Helical; Potential
Topological domain171 – 1777Cytoplasmic Potential
Transmembrane178 – 19821Helical; Potential
Topological domain199 – 2068Extracellular Potential
Transmembrane207 – 22721Helical; Potential
Topological domain228 – 2358Cytoplasmic Potential
Transmembrane236 – 25621Helical; Potential
Topological domain257 – 2648Extracellular Potential
Transmembrane265 – 28521Helical; Potential
Topological domain286 – 32843Cytoplasmic Potential
Transmembrane329 – 34921Helical; Potential
Topological domain350 – 36213Extracellular Potential
Transmembrane363 – 38321Helical; Potential
Topological domain384 – 3929Cytoplasmic Potential
Transmembrane393 – 41321Helical; Potential
Topological domain414 – 4152Extracellular Potential
Transmembrane416 – 43621Helical; Potential
Topological domain437 – 45317Cytoplasmic Potential
Transmembrane454 – 47421Helical; Potential
Topological domain475 – 4839Extracellular Potential
Transmembrane484 – 50421Helical; Potential
Topological domain505 – 54541Cytoplasmic Potential
Repeat29 – 50221
Repeat51 – 72222
Region29 – 72442 X 22 AA approximate tandem repeats

Amino acid modifications

Modified residue21N-acetylalanine By similarity

Experimental info

Sequence conflict621Q → QPLPDPAPLPELGFEAEPEP Q Ref.1
Sequence conflict2271G → D in AAC40078. Ref.1

Sequences

Sequence LengthMass (Da)Tools
O70324 [UniParc].

Last modified September 5, 2006. Version 2.
Checksum: ACCC7EC6B902A6DE

FASTA54560,025
        10         20         30         40         50         60 
MALPSPASEE AEGPCQEANQ EYQEPVCSPV PEPEPEPEPE PEPDPEPVPV PPPEPQPEPE 

        70         80         90        100        110        120 
PQPLPDPAPL PELGFEAEPV QEPEPTPTVE TRGTARGFQP PEGGFGWIVV FAATWCNGSI 

       130        140        150        160        170        180 
FGIHNSVGIL YSMLLEEEKE KNRQVEFQAA WVGALAMGMI FFCSPIVSIF TDRLGCRITA 

       190        200        210        220        230        240 
TTGAAVAFIG LHTSSFTSSL SLRYFTYGIL FGCGCSFAFQ PSLVILGHYF QRRLGLANGV 

       250        260        270        280        290        300 
VSAGSSIFSM SFPFLIKMLG DKIKLAQTFQ VLSTFMFVLT LLSLTYRPLL PSSQDTPSKR 

       310        320        330        340        350        360 
GAHTLRQRFL VQFRKYFNMR VFRQRTYRIW AFGIAAAALG YFVPYVHLMK YVEDKFKEIK 

       370        380        390        400        410        420 
ETWVLLVCIG ATSGLGRLVS GHISDSIPGL KKIYLQVLSF LLLGLMSMMI PLCRDFGGLI 

       430        440        450        460        470        480 
VVCLFLGLCD GFFITIMAPI AFELVGPMQA SQAIGYLLGM MALPMIAGPP IAGLLRNCFG 

       490        500        510        520        530        540 
DYHVAFYFAG VPPIIGAVIL FFVPLMHQRM FKKEQRDSSK DKMLSHDPDP NGELLPGSPT 


PEEPI 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and localization of the murine Xpct gene: evidence for complex rearrangements during the evolution of the region around the Xist gene."
Debrand E., Heard E., Avner P.
Genomics 48:296-303(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Liver.
[2]"Comparative sequence analysis of the X-inactivation center region in mouse, human and bovine."
Chureau C., Prissette M., Bourdet A., Barbe V., Cattolico L., Jones L., Eggen A., Avner P., Duret L.
Genome Res. 12:894-908(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: 129/Sv.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6.
Tissue: Olfactory epithelium.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AF045692 mRNA. Translation: AAC40078.1.
AJ421478 Genomic DNA. Translation: CAD33931.1.
BC080678 mRNA. Translation: AAH80678.1.
RefSeqNP_033223.2. NM_009197.2.
UniGeneMm.388973.

3D structure databases

ProteinModelPortalO70324.
SMRO70324. Positions 228-262, 356-388.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

TCDB2.A.1.13.3. the major facilitator superfamily (mfs).

PTM databases

PhosphoSiteO70324.

Proteomic databases

PaxDbO70324.
PRIDEO70324.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000042664; ENSMUSP00000037629; ENSMUSG00000033965.
GeneID20502.
KEGGmmu:20502.
UCSCuc009tzy.1. mouse.

Organism-specific databases

CTD6567.
MGIMGI:1203732. Slc16a2.

Phylogenomic databases

eggNOGNOG321880.
HOGENOMHOG000046644.
HOVERGENHBG006387.
InParanoidO70324.
KOK08231.
OMAIKQTWVL.
OrthoDBEOG7KWSH8.
PhylomeDBO70324.
TreeFamTF313792.

Gene expression databases

ArrayExpressO70324.
BgeeO70324.
GenevestigatorO70324.

Family and domain databases

InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSSLC16A2. mouse.
NextBio298675.
PROO70324.
SOURCESearch...

Entry information

Entry nameMOT8_MOUSE
AccessionPrimary (citable) accession number: O70324
Secondary accession number(s): Q8K3S9
Entry history
Integrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: September 5, 2006
Last modified: April 16, 2014
This is version 105 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot