Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

O70320

- PLB1_CAVPO

UniProt

O70320 - PLB1_CAVPO

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Phospholipase B1, membrane-associated

Gene

PLB1

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity).By similarity

Catalytic activityi

2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

Enzyme regulationi

Inhibited by diisopropyl fluorophosphate.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei399 – 3991NucleophileBy similarity
Binding sitei473 – 4731Substrate; via amide nitrogenBy similarity
Binding sitei512 – 5121SubstrateBy similarity
Active sitei1347 – 13471By similarity
Active sitei1350 – 13501By similarity

GO - Molecular functioni

  1. lysophospholipase activity Source: UniProtKB-EC
  2. phospholipase A2 activity Source: UniProtKB-EC

GO - Biological processi

  1. lipid catabolic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase B1, membrane-associated
Short name:
Phospholipase B
Alternative name(s):
Phospholipase B/lipase
Short name:
PLB/LIP
Including the following 2 domains:
Phospholipase A2 (EC:3.1.1.4)
Lysophospholipase (EC:3.1.1.5)
Gene namesi
Name:PLB1
Synonyms:PLB
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
ProteomesiUP000005447: Unplaced

Subcellular locationi

Apical cell membrane By similarity; Single-pass type I membrane protein By similarity
Note: Present in the intestinal brush border membranes.1 Publication

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Chaini21 – 14631443Phospholipase B1, membrane-associatedPRO_0000324382Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis
Glycosylationi796 – 7961N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

Undergoes proteolytic cleavage in the ileum.1 Publication

Keywords - PTMi

Glycoprotein

Expressioni

Tissue specificityi

Expressed in the epididymis, jejunum and ileon (at protein level).3 Publications

Interactioni

Protein-protein interaction databases

STRINGi10141.ENSCPOP00000009712.

Structurei

3D structure databases

ProteinModelPortaliO70320.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 14191399ExtracellularSequence AnalysisAdd
BLAST
Topological domaini1441 – 146323CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei1420 – 144021HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati38 – 3463091Add
BLAST
Repeati361 – 7063462Add
BLAST
Repeati707 – 10533473Add
BLAST
Repeati1063 – 14033414Add
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni38 – 140313664 X 308-326 AA approximate repeatsAdd
BLAST
Regioni1404 – 145047Necessary for membrane localizationBy similarityAdd
BLAST

Domaini

Repeat 2 contains the catalytic domain.By similarity

Sequence similaritiesi

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG311176.
HOGENOMiHOG000115574.
HOVERGENiHBG108263.
InParanoidiO70320.

Family and domain databases

Gene3Di3.40.50.1110. 4 hits.
InterProiIPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view]
PfamiPF00657. Lipase_GDSL. 3 hits.
[Graphical view]
PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

O70320-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGLQPGVLLV GLLLLGQGIT QIHTSSGKST LEGQLWPETK KNFPFSCSPK
60 70 80 90 100
KLGLNMPSES VHTLTPADIK LIAAIGDMET PPDSGAVNLD TSERTEKEPW
110 120 130 140 150
RGCMGMMTVL SDIISHFNPS VLLPTCPPWR SAAVRGGVEE LRTQAEELVS
160 170 180 190 200
SLKKNPQLDF QQDWKLINVF FSNASLCYLC PSAHENGPLM SNMDKLAGIL
210 220 230 240 250
HYLHQEVPRA FVNLVDLFEV VAMPRWHQGT MLSRPSPEAC GCSGETSKLD
260 270 280 290 300
TVVMQWSYQE TWDSLLASSS FNDQESFAVV FQPFFYEVSS PVEEPPSQDP
310 320 330 340 350
TTLALSLWNN MMKPVGQKDE PFSTIERRPM KCPSQESPYL FTYRNSNYQS
360 370 380 390 400
RLLKRQRQHK EREGTEIRCP DKDPSDSTPT SVHRLKPADI KVIGALGDSL
410 420 430 440 450
TAGNGAGSRP GNILDVLTEY RGLSWSIGAD HNISSVTTLP NILREFNPSL
460 470 480 490 500
KGFSTGTGKA NSVGAFFNQA VAGARAGDLI PQARTLVDLM KNHTSINFEE
510 520 530 540 550
DWKIITVFIG GNDLCDFCSD PVTNSPENFT DNIRQALDIL HAEVPRAFVN
560 570 580 590 600
MVKVLQIVNL RELYKDSRVS CPRLILRNLC RCVLLPDDNS TELESLIDIN
610 620 630 640 650
KKYQERTHQL IESGRYDTRE DFTVVLQPFF EKVDIPKTSE GLPDNTSFAP
660 670 680 690 700
DCFHFSSKTH ARAASALWKN MLEPVGQKTT QNNFENSIDI ICPNQAFPYL
710 720 730 740 750
STYKNGIEGH GTWLTCRERT PSASPPTSVH ALRPADVRVV AALGDSLTAG
760 770 780 790 800
SGIGSKPGDL ADVITQYRGL SYSSGGDGSL MNVTTLPNIL REFNSNLTGY
810 820 830 840 850
AVGTGDASNT NAFLNQAVPG AKAEELMSQV KTLVQKMKDD PRINFHEDWK
860 870 880 890 900
VITVLIGTND LCNHCTDLDL YSSANFFNHL LNALDILHRE VPRALVNLVD
910 920 930 940 950
FMNPSIMRQV FLGNPDKCPV QQASILCNCV LSLRENSYEL ARMDALTRAY
960 970 980 990 1000
QSSMRELVES GRYDTREDFS VVLQPFFLNI RLPILEDGRP DTSFFAPDCI
1010 1020 1030 1040 1050
NPGQKFHSQL SRALWVNMLE PVGSKTDTLD LTADISLPCP TQEEPFLRTP
1060 1070 1080 1090 1100
QNSDYTYPTK PAIENWGSDF LCTEWKPSNS VPTSVHKLQP ADIKVVAALG
1110 1120 1130 1140 1150
DSLTTAVGAR ASNSSDLLMS WRGLSWSIGG DGALETHTTL PNILKKFNPS
1160 1170 1180 1190 1200
IFGFSTGTLE ETAGFNVAVE EARARDMPAQ ARDLVERMKA STEINLEMDW
1210 1220 1230 1240 1250
KLITLFIGSN DLCHYCDNPE NHSAEEYVQH IRQALDILYE ELPRAFINVV
1260 1270 1280 1290 1300
DIIMELAGLH QGQGGHCTAL LPAQSTCSCL RHFPSSPVIQ ELKKVTWNLQ
1310 1320 1330 1340 1350
SDMSRLSYQE KYTQREDFAV VVQPFFQNTL IPLDKLGSTD PTFFSEDCLH
1360 1370 1380 1390 1400
FSERGHAEMA IALWNNMLEP VGHKTTFNNF TYNRTKLKCP STESPYLYTL
1410 1420 1430 1440 1450
QNSLSLPVQT EKASGVAPGI VSAAAAGGLL VGLIVGILAV SLWSSFRRRQ
1460
KKSPPESVPV ANF
Length:1,463
Mass (Da):162,176
Last modified:August 1, 1998 - v1
Checksum:i101C4969815F24B1
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF045454 mRNA. Translation: AAC40129.1.
RefSeqiNP_001166499.1. NM_001173028.1.

Genome annotation databases

GeneIDi100135633.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
AF045454 mRNA. Translation: AAC40129.1 .
RefSeqi NP_001166499.1. NM_001173028.1.

3D structure databases

ProteinModelPortali O70320.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 10141.ENSCPOP00000009712.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 100135633.

Organism-specific databases

CTDi 100135633.

Phylogenomic databases

eggNOGi NOG311176.
HOGENOMi HOG000115574.
HOVERGENi HBG108263.
InParanoidi O70320.

Family and domain databases

Gene3Di 3.40.50.1110. 4 hits.
InterProi IPR001087. Lipase_GDSL.
IPR008265. Lipase_GDSL_AS.
IPR013831. SGNH_hydro-type_esterase_dom.
[Graphical view ]
Pfami PF00657. Lipase_GDSL. 3 hits.
[Graphical view ]
PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Ectopic epididymal expression of guinea pig intestinal phospholipase B. Possible role in sperm maturation and activation by limited proteolytic digestion."
    Delagebeaudeuf C., Gassama-Diagne A., Nauze M., Ragab A., Li R.Y., Capdevielle J., Ferrara P., Fauvel J., Chap H.
    J. Biol. Chem. 273:13407-13414(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 396-402; 463-470 AND 895-904, PROTEOLYTIC PROCESSING, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
    Strain: Crl:(BFA)BR.
    Tissue: Intestine.
  2. "Purification of a new, calcium-independent, high molecular weight phospholipase A2/lysophospholipase (phospholipase B) from guinea pig intestinal brush-border membrane."
    Gassama-Diagne A., Fauvel J., Chap H.
    J. Biol. Chem. 264:9470-9475(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, TISSUE SPECIFICITY.
  3. "Further characterization of a novel phospholipase B (phospholipase A2-lysophospholipase) from intestinal brush-border membranes."
    Pind S., Kuksis A.
    Biochem. Cell Biol. 69:346-357(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.

Entry informationi

Entry nameiPLB1_CAVPO
AccessioniPrimary (citable) accession number: O70320
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 18, 2008
Last sequence update: August 1, 1998
Last modified: October 29, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3