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O70320

- PLB1_CAVPO

UniProt

O70320 - PLB1_CAVPO

Protein

Phospholipase B1, membrane-associated

Gene

PLB1

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 1 (01 Aug 1998)
      Previous versions | rss
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    Functioni

    Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids By similarity.By similarity

    Catalytic activityi

    2-lysophosphatidylcholine + H2O = glycerophosphocholine + a carboxylate.
    Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.

    Enzyme regulationi

    Inhibited by diisopropyl fluorophosphate.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei399 – 3991NucleophileBy similarity
    Binding sitei473 – 4731Substrate; via amide nitrogenBy similarity
    Binding sitei512 – 5121SubstrateBy similarity
    Active sitei1347 – 13471By similarity
    Active sitei1350 – 13501By similarity

    GO - Molecular functioni

    1. lysophospholipase activity Source: UniProtKB-EC
    2. phospholipase A2 activity Source: UniProtKB-EC

    GO - Biological processi

    1. lipid catabolic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Lipid degradation, Lipid metabolism

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase B1, membrane-associated
    Short name:
    Phospholipase B
    Alternative name(s):
    Phospholipase B/lipase
    Short name:
    PLB/LIP
    Including the following 2 domains:
    Phospholipase A2 (EC:3.1.1.4)
    Lysophospholipase (EC:3.1.1.5)
    Gene namesi
    Name:PLB1
    Synonyms:PLB
    OrganismiCavia porcellus (Guinea pig)
    Taxonomic identifieri10141 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricognathiCaviidaeCavia
    ProteomesiUP000005447: Unplaced

    Subcellular locationi

    Apical cell membrane By similarity; Single-pass type I membrane protein By similarity
    Note: Present in the intestinal brush border membranes.1 Publication

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB-SubCell
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Chaini21 – 14631443Phospholipase B1, membrane-associatedPRO_0000324382Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi528 – 5281N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi796 – 7961N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    Undergoes proteolytic cleavage in the ileum.1 Publication

    Keywords - PTMi

    Glycoprotein

    Expressioni

    Tissue specificityi

    Expressed in the epididymis, jejunum and ileon (at protein level).3 Publications

    Interactioni

    Protein-protein interaction databases

    STRINGi10141.ENSCPOP00000009712.

    Structurei

    3D structure databases

    ProteinModelPortaliO70320.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini21 – 14191399ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini1441 – 146323CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei1420 – 144021HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Repeati38 – 3463091Add
    BLAST
    Repeati361 – 7063462Add
    BLAST
    Repeati707 – 10533473Add
    BLAST
    Repeati1063 – 14033414Add
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni38 – 140313664 X 308-326 AA approximate repeatsAdd
    BLAST
    Regioni1404 – 145047Necessary for membrane localizationBy similarityAdd
    BLAST

    Domaini

    Repeat 2 contains the catalytic domain.By similarity

    Sequence similaritiesi

    Keywords - Domaini

    Repeat, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG311176.
    HOGENOMiHOG000115574.
    HOVERGENiHBG108263.
    InParanoidiO70320.

    Family and domain databases

    Gene3Di3.40.50.1110. 4 hits.
    InterProiIPR001087. Lipase_GDSL.
    IPR008265. Lipase_GDSL_AS.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view]
    PfamiPF00657. Lipase_GDSL. 3 hits.
    [Graphical view]
    PROSITEiPS01098. LIPASE_GDSL_SER. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    O70320-1 [UniParc]FASTAAdd to Basket

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    MGLQPGVLLV GLLLLGQGIT QIHTSSGKST LEGQLWPETK KNFPFSCSPK     50
    KLGLNMPSES VHTLTPADIK LIAAIGDMET PPDSGAVNLD TSERTEKEPW 100
    RGCMGMMTVL SDIISHFNPS VLLPTCPPWR SAAVRGGVEE LRTQAEELVS 150
    SLKKNPQLDF QQDWKLINVF FSNASLCYLC PSAHENGPLM SNMDKLAGIL 200
    HYLHQEVPRA FVNLVDLFEV VAMPRWHQGT MLSRPSPEAC GCSGETSKLD 250
    TVVMQWSYQE TWDSLLASSS FNDQESFAVV FQPFFYEVSS PVEEPPSQDP 300
    TTLALSLWNN MMKPVGQKDE PFSTIERRPM KCPSQESPYL FTYRNSNYQS 350
    RLLKRQRQHK EREGTEIRCP DKDPSDSTPT SVHRLKPADI KVIGALGDSL 400
    TAGNGAGSRP GNILDVLTEY RGLSWSIGAD HNISSVTTLP NILREFNPSL 450
    KGFSTGTGKA NSVGAFFNQA VAGARAGDLI PQARTLVDLM KNHTSINFEE 500
    DWKIITVFIG GNDLCDFCSD PVTNSPENFT DNIRQALDIL HAEVPRAFVN 550
    MVKVLQIVNL RELYKDSRVS CPRLILRNLC RCVLLPDDNS TELESLIDIN 600
    KKYQERTHQL IESGRYDTRE DFTVVLQPFF EKVDIPKTSE GLPDNTSFAP 650
    DCFHFSSKTH ARAASALWKN MLEPVGQKTT QNNFENSIDI ICPNQAFPYL 700
    STYKNGIEGH GTWLTCRERT PSASPPTSVH ALRPADVRVV AALGDSLTAG 750
    SGIGSKPGDL ADVITQYRGL SYSSGGDGSL MNVTTLPNIL REFNSNLTGY 800
    AVGTGDASNT NAFLNQAVPG AKAEELMSQV KTLVQKMKDD PRINFHEDWK 850
    VITVLIGTND LCNHCTDLDL YSSANFFNHL LNALDILHRE VPRALVNLVD 900
    FMNPSIMRQV FLGNPDKCPV QQASILCNCV LSLRENSYEL ARMDALTRAY 950
    QSSMRELVES GRYDTREDFS VVLQPFFLNI RLPILEDGRP DTSFFAPDCI 1000
    NPGQKFHSQL SRALWVNMLE PVGSKTDTLD LTADISLPCP TQEEPFLRTP 1050
    QNSDYTYPTK PAIENWGSDF LCTEWKPSNS VPTSVHKLQP ADIKVVAALG 1100
    DSLTTAVGAR ASNSSDLLMS WRGLSWSIGG DGALETHTTL PNILKKFNPS 1150
    IFGFSTGTLE ETAGFNVAVE EARARDMPAQ ARDLVERMKA STEINLEMDW 1200
    KLITLFIGSN DLCHYCDNPE NHSAEEYVQH IRQALDILYE ELPRAFINVV 1250
    DIIMELAGLH QGQGGHCTAL LPAQSTCSCL RHFPSSPVIQ ELKKVTWNLQ 1300
    SDMSRLSYQE KYTQREDFAV VVQPFFQNTL IPLDKLGSTD PTFFSEDCLH 1350
    FSERGHAEMA IALWNNMLEP VGHKTTFNNF TYNRTKLKCP STESPYLYTL 1400
    QNSLSLPVQT EKASGVAPGI VSAAAAGGLL VGLIVGILAV SLWSSFRRRQ 1450
    KKSPPESVPV ANF 1463
    Length:1,463
    Mass (Da):162,176
    Last modified:August 1, 1998 - v1
    Checksum:i101C4969815F24B1
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF045454 mRNA. Translation: AAC40129.1.
    RefSeqiNP_001166499.1. NM_001173028.1.

    Genome annotation databases

    GeneIDi100135633.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AF045454 mRNA. Translation: AAC40129.1 .
    RefSeqi NP_001166499.1. NM_001173028.1.

    3D structure databases

    ProteinModelPortali O70320.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10141.ENSCPOP00000009712.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 100135633.

    Organism-specific databases

    CTDi 100135633.

    Phylogenomic databases

    eggNOGi NOG311176.
    HOGENOMi HOG000115574.
    HOVERGENi HBG108263.
    InParanoidi O70320.

    Family and domain databases

    Gene3Di 3.40.50.1110. 4 hits.
    InterProi IPR001087. Lipase_GDSL.
    IPR008265. Lipase_GDSL_AS.
    IPR013831. SGNH_hydro-type_esterase_dom.
    [Graphical view ]
    Pfami PF00657. Lipase_GDSL. 3 hits.
    [Graphical view ]
    PROSITEi PS01098. LIPASE_GDSL_SER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Ectopic epididymal expression of guinea pig intestinal phospholipase B. Possible role in sperm maturation and activation by limited proteolytic digestion."
      Delagebeaudeuf C., Gassama-Diagne A., Nauze M., Ragab A., Li R.Y., Capdevielle J., Ferrara P., Fauvel J., Chap H.
      J. Biol. Chem. 273:13407-13414(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 396-402; 463-470 AND 895-904, PROTEOLYTIC PROCESSING, TISSUE SPECIFICITY, SUBCELLULAR LOCATION.
      Strain: Crl:(BFA)BR.
      Tissue: Intestine.
    2. "Purification of a new, calcium-independent, high molecular weight phospholipase A2/lysophospholipase (phospholipase B) from guinea pig intestinal brush-border membrane."
      Gassama-Diagne A., Fauvel J., Chap H.
      J. Biol. Chem. 264:9470-9475(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, TISSUE SPECIFICITY.
    3. "Further characterization of a novel phospholipase B (phospholipase A2-lysophospholipase) from intestinal brush-border membranes."
      Pind S., Kuksis A.
      Biochem. Cell Biol. 69:346-357(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiPLB1_CAVPO
    AccessioniPrimary (citable) accession number: O70320
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: March 18, 2008
    Last sequence update: August 1, 1998
    Last modified: October 1, 2014
    This is version 70 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3