Reviewed, UniProtKB/Swiss-Prot O70311 (NMT2_MOUSE)

Names and origin

Protein names

Recommended name:
    Glycylpeptide N-tetradecanoyltransferase 2
    EC=2.3.1.97
Alternative name(s):
    Peptide N-myristoyltransferase 2
    Myristoyl-CoA:protein N-myristoyltransferase 2
      Short name=NMT 2
    Type II N-myristoyltransferase

Gene names

Name:

Nmt2

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	529 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Adds a myristoyl group to the N-terminal glycine residue of certain cellular and viral proteins.
Catalytic activity	Tetradecanoyl-CoA + glycylpeptide = CoA + N-tetradecanoylglycylpeptide.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the NMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	N-terminal protein myristoylation Traceable author statement. Source: MGI
Cellular component	cytoplasm Ref.1 Traceable author statement. Source: MGI
Molecular function	glycylpeptide N-tetradecanoyltransferase activity Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 529	529	Glycylpeptide N-tetradecanoyltransferase 2		PRO_0000064227
Regions
	Compositional bias	46 – 56	11	Poly-Lys
Amino acid modifications
	Modified residue	66	1	Phosphoserine Ref.2
	Modified residue	68	1	Phosphoserine Ref.2
	Modified residue	70	1	Phosphoserine Ref.2

Sequences

	Sequence		Length	Mass (Da)
	O70311-1 [UniParc]. Last modified August 1, 1998. Version 1. Checksum: 7D5C93D9159F315C	FASTA	529	60,484
	10 20 30 40 50 60 MAEDSESAAS QQSLELDDQD TCGIDGDNEE ETEHAKGSPG GDLGAKKKKK KQKRKKEKPN 70 80 90 100 110 120 SGGTKSDSAS DSQEIKIQQS SKHNAIWQQI SAGAAMGGDT MEGEWIDLRM YHKNPTIPIQ 130 140 150 160 170 180 KLQDIQRAME LLSACQGPAR NIDEATKRRY QFWDTQPVPK LNEVITSHGA IEPDKDNIRQ 190 200 210 220 230 240 EPYSLPQGFM WDTLDLSNAE VLKELYTLLN ENYVEDDDNM FRFDYSPEFL LWALRPPGWL 250 260 270 280 290 300 LQWHCGVRVS SNKKLVGFIS AIPANIRIYD SVKRMVEINF LCVHKKLRSK RVAPVLIREI 310 320 330 340 350 360 TRRVNLEGIF QAVYTAGVVL PKPVATCRYW HRSLNPRKLV EVKFSHLSRN MTLQRTMKLY 370 380 390 400 410 420 RLPDVTKTSG LRPMEPKDIK AVRELINIYL KQFHLAPVMD DAEVAHWFLP REHIIDTFVV 430 440 450 460 470 480 ENPSGKLTDF LSFYTLPSTV MHHPAHKSLK AAYSFYNIHT ETPLLDLMND ALIIAKLKGF 490 500 510 520 DVFNALDLME NKTFLEKLKF GIGDGNLQYY LYNWRCPGTD SEKVGLVLQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A second mammalian N-myristoyltransferase." Giang D.K., Cravatt B.F. J. Biol. Chem. 273:6595-6598(1998) [PubMed: 9506952] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Solid tumor proteome and phosphoproteome analysis by high resolution mass spectrometry." Zanivan S., Gnad F., Wickstroem S.A., Geiger T., Macek B., Cox J., Faessler R., Mann M. J. Proteome Res. 7:5314-5326(2008) [PubMed: 18973353] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-66; SER-68 AND SER-70, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AF043327 mRNA. Translation: AAC09297.1.
IPI	IPI00117252.
RefSeq	NP_032734.1.
UniGene	Mm.392918 Mm.65021
3D structure databases
HSSP	HSSP built from PDB template 1NMT based on UniProtKB P30418.
ModBase	Search...
Protein-protein interaction databases
STRING	O70311.
PTM databases
PhosphoSite	O70311.
Proteomic databases
PRIDE	O70311.
Genome annotation databases
Ensembl	ENSMUST00000081932; ENSMUSP00000080600; ENSMUSG00000026643; Mus musculus. [Genome view]
GeneID	18108.
KEGG	mmu:18108.
UCSC	uc008idq.1. mouse.
Organism-specific databases
CTD	18108.
MGI	MGI:1202298. Nmt2.
Phylogenomic databases
HOGENOM	O70311.
HOVERGEN	O70311.
OMA	VEDDDSM.
Enzyme and pathway databases
BRENDA	2.3.1.97. 244.
Gene expression databases
ArrayExpress	O70311.
Bgee	O70311.
CleanEx	MM_NMT2.
Genevestigator	O70311.
GermOnline	ENSMUSG00000026643. Mus musculus.
Family and domain databases
InterPro	IPR016181. Acyl_CoA_acyltransferase. IPR000903. Myristoyl_trans. [Graphical view]
Gene3D	G3DSA:3.40.630.30. Acyl_CoA_acyltransferase. 2 hits.
PANTHER	PTHR11377. Myristoyl_trans. 1 hit.
Pfam	PF01233. NMT. 1 hit. PF02799. NMT_C. 1 hit. [Graphical view]
PIRSF	PIRSF015892. N-myristl_transf. 1 hit.
PROSITE	PS00975. NMT_1. 1 hit. PS00976. NMT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	293297.
SOURCE	Search...

Entry information

Entry name

NMT2_MOUSE

Accession

Primary (citable) accession number: O70311

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2000
Last sequence update:	August 1, 1998
Last modified:	November 3, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)